PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen‐fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains resu...
Gespeichert in:
| Veröffentlicht in: | Biochemistry and molecular biology education 2012-03, Vol.40 (2), p.108-111 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 111 |
|---|---|
| container_issue | 2 |
| container_start_page | 108 |
| container_title | Biochemistry and molecular biology education |
| container_volume | 40 |
| creator | Sorroche, Fernando G. Giordano, Walter |
| description | Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen‐fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low‐molecular‐weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non‐functional expR loci in S. meliloti. |
| doi_str_mv | 10.1002/bmb.20560 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_928907382</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><ericid>EJ990930</ericid><sourcerecordid>928907382</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3800-97d6b331805292616a01e7cbe6c848e41b8bc7760a72505f74424c820368e40f3</originalsourceid><addsrcrecordid>eNp1kM1P20AUxFeoqATaA_eq2lvFwfD2w97dYxKFLwVRpXC21ptnspXtTb2xIPz1mDrkxuk9aX4zIw0hpwzOGQC_KOrinEOawQEZsVSYREhuvvS_VJBoptIjchzjX-jZTKqv5IhzyUxq2IjY39MFHTe22kYfaSgpvqwX9AobpAt86iq78c0TnfgQt81mhTto9hLWobdY51a29UuM1Df0j29Cu_KvofBdTWusfBU2_hs5LG0V8fvunpDHy9nD9DqZ31_dTMfzxAkNkBi1zAohmIaUG56xzAJD5QrMnJYaJSt04ZTKwCqeQloqKbl0moPIehVKcUJ-DbnrNvzrMG7y2keHVWUbDF3MDdcGlNC8J88G0rUhxhbLfN362rbbnEH-PmjeD5r_H7Rnf-5Su6LG5Z78WLAHfgwAtt7t5dmtMWDEu_9ikJ99hdvPi_LJ3WRofAOQG4fF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>928907382</pqid></control><display><type>article</type><title>PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti</title><source>Wiley Free Content</source><source>MEDLINE</source><source>Education Source</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Sorroche, Fernando G. ; Giordano, Walter</creator><creatorcontrib>Sorroche, Fernando G. ; Giordano, Walter</creatorcontrib><description>Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen‐fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low‐molecular‐weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non‐functional expR loci in S. meliloti.</description><identifier>ISSN: 1470-8175</identifier><identifier>EISSN: 1539-3429</identifier><identifier>DOI: 10.1002/bmb.20560</identifier><identifier>PMID: 22419591</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Argentina ; Bacterial Proteins - genetics ; Biochemistry ; Biochemistry - education ; College Science ; Curriculum ; Electrophoresis, Agar Gel ; exopolysaccharides ; expR gen ; Foreign Countries ; Gene Expression Regulation, Bacterial ; Genetics ; Molecular Structure ; PCR ; Plants (Botany) ; Polymerase Chain Reaction ; Polysaccharides, Bacterial - biosynthesis ; Quorum Sensing ; rhizobacteria ; Science Experiments ; Science Instruction ; Science Laboratories ; Sinorhizobium meliloti - genetics ; Sinorhizobium meliloti - metabolism ; Teaching Methods ; Trans-Activators - genetics ; Undergraduate Study</subject><ispartof>Biochemistry and molecular biology education, 2012-03, Vol.40 (2), p.108-111</ispartof><rights>Copyright © 2011 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3800-97d6b331805292616a01e7cbe6c848e41b8bc7760a72505f74424c820368e40f3</citedby><cites>FETCH-LOGICAL-c3800-97d6b331805292616a01e7cbe6c848e41b8bc7760a72505f74424c820368e40f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmb.20560$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmb.20560$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://eric.ed.gov/ERICWebPortal/detail?accno=EJ990930$$DView record in ERIC$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22419591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sorroche, Fernando G.</creatorcontrib><creatorcontrib>Giordano, Walter</creatorcontrib><title>PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti</title><title>Biochemistry and molecular biology education</title><addtitle>Biochem Mol Biol Educ</addtitle><description>Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen‐fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low‐molecular‐weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non‐functional expR loci in S. meliloti.</description><subject>Argentina</subject><subject>Bacterial Proteins - genetics</subject><subject>Biochemistry</subject><subject>Biochemistry - education</subject><subject>College Science</subject><subject>Curriculum</subject><subject>Electrophoresis, Agar Gel</subject><subject>exopolysaccharides</subject><subject>expR gen</subject><subject>Foreign Countries</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetics</subject><subject>Molecular Structure</subject><subject>PCR</subject><subject>Plants (Botany)</subject><subject>Polymerase Chain Reaction</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Quorum Sensing</subject><subject>rhizobacteria</subject><subject>Science Experiments</subject><subject>Science Instruction</subject><subject>Science Laboratories</subject><subject>Sinorhizobium meliloti - genetics</subject><subject>Sinorhizobium meliloti - metabolism</subject><subject>Teaching Methods</subject><subject>Trans-Activators - genetics</subject><subject>Undergraduate Study</subject><issn>1470-8175</issn><issn>1539-3429</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1P20AUxFeoqATaA_eq2lvFwfD2w97dYxKFLwVRpXC21ptnspXtTb2xIPz1mDrkxuk9aX4zIw0hpwzOGQC_KOrinEOawQEZsVSYREhuvvS_VJBoptIjchzjX-jZTKqv5IhzyUxq2IjY39MFHTe22kYfaSgpvqwX9AobpAt86iq78c0TnfgQt81mhTto9hLWobdY51a29UuM1Df0j29Cu_KvofBdTWusfBU2_hs5LG0V8fvunpDHy9nD9DqZ31_dTMfzxAkNkBi1zAohmIaUG56xzAJD5QrMnJYaJSt04ZTKwCqeQloqKbl0moPIehVKcUJ-DbnrNvzrMG7y2keHVWUbDF3MDdcGlNC8J88G0rUhxhbLfN362rbbnEH-PmjeD5r_H7Rnf-5Su6LG5Z78WLAHfgwAtt7t5dmtMWDEu_9ikJ99hdvPi_LJ3WRofAOQG4fF</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>Sorroche, Fernando G.</creator><creator>Giordano, Walter</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>7SW</scope><scope>BJH</scope><scope>BNH</scope><scope>BNI</scope><scope>BNJ</scope><scope>BNO</scope><scope>ERI</scope><scope>PET</scope><scope>REK</scope><scope>WWN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201203</creationdate><title>PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti</title><author>Sorroche, Fernando G. ; Giordano, Walter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3800-97d6b331805292616a01e7cbe6c848e41b8bc7760a72505f74424c820368e40f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Argentina</topic><topic>Bacterial Proteins - genetics</topic><topic>Biochemistry</topic><topic>Biochemistry - education</topic><topic>College Science</topic><topic>Curriculum</topic><topic>Electrophoresis, Agar Gel</topic><topic>exopolysaccharides</topic><topic>expR gen</topic><topic>Foreign Countries</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetics</topic><topic>Molecular Structure</topic><topic>PCR</topic><topic>Plants (Botany)</topic><topic>Polymerase Chain Reaction</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Quorum Sensing</topic><topic>rhizobacteria</topic><topic>Science Experiments</topic><topic>Science Instruction</topic><topic>Science Laboratories</topic><topic>Sinorhizobium meliloti - genetics</topic><topic>Sinorhizobium meliloti - metabolism</topic><topic>Teaching Methods</topic><topic>Trans-Activators - genetics</topic><topic>Undergraduate Study</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sorroche, Fernando G.</creatorcontrib><creatorcontrib>Giordano, Walter</creatorcontrib><collection>ERIC</collection><collection>ERIC (Ovid)</collection><collection>ERIC</collection><collection>ERIC</collection><collection>ERIC (Legacy Platform)</collection><collection>ERIC( SilverPlatter )</collection><collection>ERIC</collection><collection>ERIC PlusText (Legacy Platform)</collection><collection>Education Resources Information Center (ERIC)</collection><collection>ERIC</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry and molecular biology education</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sorroche, Fernando G.</au><au>Giordano, Walter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><ericid>EJ990930</ericid><atitle>PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti</atitle><jtitle>Biochemistry and molecular biology education</jtitle><addtitle>Biochem Mol Biol Educ</addtitle><date>2012-03</date><risdate>2012</risdate><volume>40</volume><issue>2</issue><spage>108</spage><epage>111</epage><pages>108-111</pages><issn>1470-8175</issn><eissn>1539-3429</eissn><abstract>Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen‐fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low‐molecular‐weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non‐functional expR loci in S. meliloti.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22419591</pmid><doi>10.1002/bmb.20560</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1470-8175 |
| ispartof | Biochemistry and molecular biology education, 2012-03, Vol.40 (2), p.108-111 |
| issn | 1470-8175 1539-3429 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_928907382 |
| source | Wiley Free Content; MEDLINE; Education Source; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Argentina Bacterial Proteins - genetics Biochemistry Biochemistry - education College Science Curriculum Electrophoresis, Agar Gel exopolysaccharides expR gen Foreign Countries Gene Expression Regulation, Bacterial Genetics Molecular Structure PCR Plants (Botany) Polymerase Chain Reaction Polysaccharides, Bacterial - biosynthesis Quorum Sensing rhizobacteria Science Experiments Science Instruction Science Laboratories Sinorhizobium meliloti - genetics Sinorhizobium meliloti - metabolism Teaching Methods Trans-Activators - genetics Undergraduate Study |
| title | PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T20%3A49%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PCR%20Analysis%20of%20expR%20Gene%20Regulating%20Biosynthesis%20of%20Exopolysaccharides%20in%20Sinorhizobium%20meliloti&rft.jtitle=Biochemistry%20and%20molecular%20biology%20education&rft.au=Sorroche,%20Fernando%20G.&rft.date=2012-03&rft.volume=40&rft.issue=2&rft.spage=108&rft.epage=111&rft.pages=108-111&rft.issn=1470-8175&rft.eissn=1539-3429&rft_id=info:doi/10.1002/bmb.20560&rft_dat=%3Cproquest_cross%3E928907382%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=928907382&rft_id=info:pmid/22419591&rft_ericid=EJ990930&rfr_iscdi=true |