Production of mouse granulocyte-macrophage colony-stimulating factor by gateway technology and transgenic rice cell culture
To establish a production platform for recombinant proteins in rice suspension cells, we first constructed a Gateway‐compatible binary T‐DNA destination vector. It provided a reliable and effective method for the rapid directional cloning of target genes into plant cells through Agrobacterium‐mediat...
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| Veröffentlicht in: | Biotechnology and bioengineering 2012-05, Vol.109 (5), p.1239-1247 |
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| creator | Liu, Yu-Kuo Huang, Li-Fen Ho, Shin-Lon Liao, Chun-Yu Liu, Hsin-Yi Lai, Ying-Hui Yu, Su-May Lu, Chung-An |
| description | To establish a production platform for recombinant proteins in rice suspension cells, we first constructed a Gateway‐compatible binary T‐DNA destination vector. It provided a reliable and effective method for the rapid directional cloning of target genes into plant cells through Agrobacterium‐mediated transformation. We used the approach to produce mouse granulocyte‐macrophage colony‐stimulating factor (mGM‐CSF) in a rice suspension cell system. The promoter for the αAmy3 amylase gene, which is induced strongly by sugar depletion, drove the expression of mGM‐CSF. The resulting recombinant protein was fused with the αAmy3 signal peptide and was secreted into the culture medium. The production of rice‐derived mGM‐CSF (rmGM‐CSF) was scaled up successfully in a 2‐L bioreactor, in which the highest yield of rmGM‐CSF was 24.6 mg/L. Due to post‐translational glycosylation, the molecular weight of rmGM‐CSF was larger than that of recombinant mGM‐CSF produced in Escherichia coli. The rmGM‐CSF was bioactive and could stimulate the proliferation of a murine myeloblastic leukemia cell line, NSF‐60. Biotechnol. Bioeng. 2012; 109:1239–1247. © 2011 Wiley Periodicals, Inc.
The authors constructed a Gateway‐compatible binary T‐DNA destination vector for the easy establishment of a production platform for recombinant proteins in rice suspension cells. Here, the 2‐L bioreactor was used for the production of recombinant mouse granulocyte‐macrophage colony‐stimulating factor (mGM‐CSF), a cytokine that functions in the growth of white blood cells, in cultured rice suspension cells. The recombinant mGM‐CSF was bioactive and could stimulate the proliferation of a murine myeloblastic leukemia cell. |
| doi_str_mv | 10.1002/bit.24394 |
| format | Article |
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The authors constructed a Gateway‐compatible binary T‐DNA destination vector for the easy establishment of a production platform for recombinant proteins in rice suspension cells. Here, the 2‐L bioreactor was used for the production of recombinant mouse granulocyte‐macrophage colony‐stimulating factor (mGM‐CSF), a cytokine that functions in the growth of white blood cells, in cultured rice suspension cells. The recombinant mGM‐CSF was bioactive and could stimulate the proliferation of a murine myeloblastic leukemia cell.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.24394</identifier><identifier>PMID: 22125231</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Agrobacterium - genetics ; Animals ; bioreactor ; Bioreactors ; Biotechnology - methods ; Cell culture ; Cell Culture Techniques ; Culture Media - chemistry ; DNA, Bacterial ; Escherichia coli ; Escherichia coli - genetics ; Gene expression ; Genetic Vectors ; Granulocyte-Macrophage Colony-Stimulating Factor - chemistry ; Granulocyte-Macrophage Colony-Stimulating Factor - genetics ; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism ; mGM-CSF ; Mice ; Molecular Weight ; Oryza - genetics ; Oryza - metabolism ; Oryza sativa ; Plants, Genetically Modified ; Promoter Regions, Genetic ; Proteins ; recombinant protein production ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; rice suspension cell ; Rodents ; Signal transduction ; sugar-depletion induced promoter ; Transgenic plants</subject><ispartof>Biotechnology and bioengineering, 2012-05, Vol.109 (5), p.1239-1247</ispartof><rights>Copyright © 2011 Wiley Periodicals, Inc.</rights><rights>Copyright John Wiley and Sons, Limited May 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4224-4ee9a33f1478ceeb93174f6148bba6a0540ad1f706e3a92bc98f6d143823f10f3</citedby><cites>FETCH-LOGICAL-c4224-4ee9a33f1478ceeb93174f6148bba6a0540ad1f706e3a92bc98f6d143823f10f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.24394$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.24394$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22125231$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yu-Kuo</creatorcontrib><creatorcontrib>Huang, Li-Fen</creatorcontrib><creatorcontrib>Ho, Shin-Lon</creatorcontrib><creatorcontrib>Liao, Chun-Yu</creatorcontrib><creatorcontrib>Liu, Hsin-Yi</creatorcontrib><creatorcontrib>Lai, Ying-Hui</creatorcontrib><creatorcontrib>Yu, Su-May</creatorcontrib><creatorcontrib>Lu, Chung-An</creatorcontrib><title>Production of mouse granulocyte-macrophage colony-stimulating factor by gateway technology and transgenic rice cell culture</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>To establish a production platform for recombinant proteins in rice suspension cells, we first constructed a Gateway‐compatible binary T‐DNA destination vector. It provided a reliable and effective method for the rapid directional cloning of target genes into plant cells through Agrobacterium‐mediated transformation. We used the approach to produce mouse granulocyte‐macrophage colony‐stimulating factor (mGM‐CSF) in a rice suspension cell system. The promoter for the αAmy3 amylase gene, which is induced strongly by sugar depletion, drove the expression of mGM‐CSF. The resulting recombinant protein was fused with the αAmy3 signal peptide and was secreted into the culture medium. The production of rice‐derived mGM‐CSF (rmGM‐CSF) was scaled up successfully in a 2‐L bioreactor, in which the highest yield of rmGM‐CSF was 24.6 mg/L. Due to post‐translational glycosylation, the molecular weight of rmGM‐CSF was larger than that of recombinant mGM‐CSF produced in Escherichia coli. The rmGM‐CSF was bioactive and could stimulate the proliferation of a murine myeloblastic leukemia cell line, NSF‐60. Biotechnol. Bioeng. 2012; 109:1239–1247. © 2011 Wiley Periodicals, Inc.
The authors constructed a Gateway‐compatible binary T‐DNA destination vector for the easy establishment of a production platform for recombinant proteins in rice suspension cells. Here, the 2‐L bioreactor was used for the production of recombinant mouse granulocyte‐macrophage colony‐stimulating factor (mGM‐CSF), a cytokine that functions in the growth of white blood cells, in cultured rice suspension cells. The recombinant mGM‐CSF was bioactive and could stimulate the proliferation of a murine myeloblastic leukemia cell.</description><subject>Agrobacterium - genetics</subject><subject>Animals</subject><subject>bioreactor</subject><subject>Bioreactors</subject><subject>Biotechnology - methods</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Culture Media - chemistry</subject><subject>DNA, Bacterial</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Gene expression</subject><subject>Genetic Vectors</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - chemistry</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - genetics</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</subject><subject>mGM-CSF</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>Oryza sativa</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>recombinant protein production</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>rice suspension cell</subject><subject>Rodents</subject><subject>Signal transduction</subject><subject>sugar-depletion induced promoter</subject><subject>Transgenic plants</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1PFTEUhhujkSu68A-Yxg2yGOjXdKZLIQokRF1c0bhpOp0zQ3Fmem07wYl_nsIFFia6OmnyvE_O6YvQa0oOKCHssHHpgAmuxBO0okRVBWGKPEUrQogseKnYDnoR41V-VrWUz9EOY5SVjNMV-vMl-Ha2yfkJ-w6Pfo6A-2CmefB2SVCMxga_uTQ9YOsHPy1FTG6cB5Pc1OPO2OQDbhbcmwTXZsEJ7OWUwX7BZmpxyqrYw-QsDs5mBwwDtvOQ5gAv0bPODBFe3c9d9PXjh_XxaXH--eTs-P15YQVjohAAynDeUVHVFqBRnFaik1TUTWOkIaUgpqVdRSRwo1hjVd3JlgpesxwiHd9Fe1vvJvhfM8SkRxdvFzET5Hu1YrUiktMyk-_-S2apIKwUnGT07V_olZ_DlO_IPsXrktYyQ_tbKP9hjAE6vQluNGHRlOjb6nSuTt9Vl9k398K5GaF9JB-6ysDhFrh2Ayz_Numjs_WDstgmXEzw-zFhwk8tK16V-tunE31Rld_XP6TSF_wG3MezfA</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Liu, Yu-Kuo</creator><creator>Huang, Li-Fen</creator><creator>Ho, Shin-Lon</creator><creator>Liao, Chun-Yu</creator><creator>Liu, Hsin-Yi</creator><creator>Lai, Ying-Hui</creator><creator>Yu, Su-May</creator><creator>Lu, Chung-An</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201205</creationdate><title>Production of mouse granulocyte-macrophage colony-stimulating factor by gateway technology and transgenic rice cell culture</title><author>Liu, Yu-Kuo ; 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Bioeng</addtitle><date>2012-05</date><risdate>2012</risdate><volume>109</volume><issue>5</issue><spage>1239</spage><epage>1247</epage><pages>1239-1247</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>To establish a production platform for recombinant proteins in rice suspension cells, we first constructed a Gateway‐compatible binary T‐DNA destination vector. It provided a reliable and effective method for the rapid directional cloning of target genes into plant cells through Agrobacterium‐mediated transformation. We used the approach to produce mouse granulocyte‐macrophage colony‐stimulating factor (mGM‐CSF) in a rice suspension cell system. The promoter for the αAmy3 amylase gene, which is induced strongly by sugar depletion, drove the expression of mGM‐CSF. The resulting recombinant protein was fused with the αAmy3 signal peptide and was secreted into the culture medium. The production of rice‐derived mGM‐CSF (rmGM‐CSF) was scaled up successfully in a 2‐L bioreactor, in which the highest yield of rmGM‐CSF was 24.6 mg/L. Due to post‐translational glycosylation, the molecular weight of rmGM‐CSF was larger than that of recombinant mGM‐CSF produced in Escherichia coli. The rmGM‐CSF was bioactive and could stimulate the proliferation of a murine myeloblastic leukemia cell line, NSF‐60. Biotechnol. Bioeng. 2012; 109:1239–1247. © 2011 Wiley Periodicals, Inc.
The authors constructed a Gateway‐compatible binary T‐DNA destination vector for the easy establishment of a production platform for recombinant proteins in rice suspension cells. Here, the 2‐L bioreactor was used for the production of recombinant mouse granulocyte‐macrophage colony‐stimulating factor (mGM‐CSF), a cytokine that functions in the growth of white blood cells, in cultured rice suspension cells. The recombinant mGM‐CSF was bioactive and could stimulate the proliferation of a murine myeloblastic leukemia cell.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22125231</pmid><doi>10.1002/bit.24394</doi><tpages>9</tpages></addata></record> |
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| subjects | Agrobacterium - genetics Animals bioreactor Bioreactors Biotechnology - methods Cell culture Cell Culture Techniques Culture Media - chemistry DNA, Bacterial Escherichia coli Escherichia coli - genetics Gene expression Genetic Vectors Granulocyte-Macrophage Colony-Stimulating Factor - chemistry Granulocyte-Macrophage Colony-Stimulating Factor - genetics Granulocyte-Macrophage Colony-Stimulating Factor - metabolism mGM-CSF Mice Molecular Weight Oryza - genetics Oryza - metabolism Oryza sativa Plants, Genetically Modified Promoter Regions, Genetic Proteins recombinant protein production Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism rice suspension cell Rodents Signal transduction sugar-depletion induced promoter Transgenic plants |
| title | Production of mouse granulocyte-macrophage colony-stimulating factor by gateway technology and transgenic rice cell culture |
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