Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method
ABSTRACT A VeraCode‐allele‐specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)‐DNA. Oligonucleotide primers containing HPV‐type‐specific L1 sequences were annealed to HPV‐DNA amplified by PGMY‐PCR, followed by ASPE to label the DNA with b...
Gespeichert in:
| Veröffentlicht in: | Microbiology and immunology 2012-02, Vol.56 (2), p.128-133 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 133 |
|---|---|
| container_issue | 2 |
| container_start_page | 128 |
| container_title | Microbiology and immunology |
| container_volume | 56 |
| creator | Kitamura-Muramatsu, Yuri Kusumoto-Matsuo, Rika Kondo, Kazunari Mori, Seiichiro Saito, Susumu Tsukahara, Yusuke Kukimoto, Iwao |
| description | ABSTRACT
A VeraCode‐allele‐specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)‐DNA. Oligonucleotide primers containing HPV‐type‐specific L1 sequences were annealed to HPV‐DNA amplified by PGMY‐PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The labeled DNA was captured by VeraCode beads through hybridization, stained with a streptavidin‐conjugated fluorophore, and detected by an Illumina BeadXpress® reader. By using this system, 16 clinically important HPV types (HPV6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68) were correctly genotyped in a multiplex format. The VeraCode‐ASPE genotyping of clinical DNA samples yielded identical results with those obtained by validated PGMY‐reverse blot hybridization assay, providing a new platform for high‐throughput genotyping required for HPV epidemiological surveys. |
| doi_str_mv | 10.1111/j.1348-0421.2011.00406.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_928372744</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>928372744</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5176-5d89de1f7981535d6851561cd52c409922f168dc814835e528149d135603f2423</originalsourceid><addsrcrecordid>eNqNkEtv1DAURi0EokPhLyDvWCX4GTsSGzSiD6kt4i2xsUx803pw4mAnJfPvSZgya7zxle537rUPQpiSki7n9a6kXOiCCEZLRigtCRGkKudHaHNsPEYbwrUsZEXICXqW844QppgWT9EJY1RURJEN6m7iPQTcTWH0Q4AZHL6FPo77wfe3OLb4bupsjwc7-BBiZ-99mjKe8tq1-Csku40OChsCBCjyAI1vfYOH5DtIGOYR-uxjjzsY76J7jp60NmR48XCfoi9n7z5vL4qr9-eX27dXRSOpqgrpdO2AtqrWVHLpKi2prGjjJGsEqWvGWlpp12gqNJcg2VLUjvLlq7xlgvFT9Oowd0jx1wR5NJ3PDYRge4hTNjXTXDElxJLUh2STYs4JWrM-3aa9ocSsrs3OrErNqtSsrs1f12Ze0JcPS6YfHbgj-E_uEnhzCPz2Afb_PdhcX14vxYIXB9znEeYjbtNPUymupPl2c27OlCKfvn_8YC74H3SSnCk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>928372744</pqid></control><display><type>article</type><title>Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method</title><source>MEDLINE</source><source>Wiley Free Content</source><source>Open Access Titles of Japan</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Kitamura-Muramatsu, Yuri ; Kusumoto-Matsuo, Rika ; Kondo, Kazunari ; Mori, Seiichiro ; Saito, Susumu ; Tsukahara, Yusuke ; Kukimoto, Iwao</creator><creatorcontrib>Kitamura-Muramatsu, Yuri ; Kusumoto-Matsuo, Rika ; Kondo, Kazunari ; Mori, Seiichiro ; Saito, Susumu ; Tsukahara, Yusuke ; Kukimoto, Iwao</creatorcontrib><description>ABSTRACT
A VeraCode‐allele‐specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)‐DNA. Oligonucleotide primers containing HPV‐type‐specific L1 sequences were annealed to HPV‐DNA amplified by PGMY‐PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The labeled DNA was captured by VeraCode beads through hybridization, stained with a streptavidin‐conjugated fluorophore, and detected by an Illumina BeadXpress® reader. By using this system, 16 clinically important HPV types (HPV6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68) were correctly genotyped in a multiplex format. The VeraCode‐ASPE genotyping of clinical DNA samples yielded identical results with those obtained by validated PGMY‐reverse blot hybridization assay, providing a new platform for high‐throughput genotyping required for HPV epidemiological surveys.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.2011.00406.x</identifier><identifier>PMID: 22146070</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>allele-specific primer extension ; Alleles ; Alphapapillomavirus - classification ; Alphapapillomavirus - genetics ; Alphapapillomavirus - isolation & purification ; DNA Primers - genetics ; Female ; Genotype ; genotyping ; human papillomavirus ; Humans ; multiplex assay ; Multiplex Polymerase Chain Reaction - instrumentation ; Multiplex Polymerase Chain Reaction - methods ; Papillomavirus Infections - diagnosis ; Papillomavirus Infections - virology</subject><ispartof>Microbiology and immunology, 2012-02, Vol.56 (2), p.128-133</ispartof><rights>2012 The Societies and Blackwell Publishing Asia Pty Ltd</rights><rights>2012 The Societies and Blackwell Publishing Asia Pty Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c5176-5d89de1f7981535d6851561cd52c409922f168dc814835e528149d135603f2423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1348-0421.2011.00406.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1348-0421.2011.00406.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22146070$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitamura-Muramatsu, Yuri</creatorcontrib><creatorcontrib>Kusumoto-Matsuo, Rika</creatorcontrib><creatorcontrib>Kondo, Kazunari</creatorcontrib><creatorcontrib>Mori, Seiichiro</creatorcontrib><creatorcontrib>Saito, Susumu</creatorcontrib><creatorcontrib>Tsukahara, Yusuke</creatorcontrib><creatorcontrib>Kukimoto, Iwao</creatorcontrib><title>Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method</title><title>Microbiology and immunology</title><addtitle>Microbiol Immunol</addtitle><description>ABSTRACT
A VeraCode‐allele‐specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)‐DNA. Oligonucleotide primers containing HPV‐type‐specific L1 sequences were annealed to HPV‐DNA amplified by PGMY‐PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The labeled DNA was captured by VeraCode beads through hybridization, stained with a streptavidin‐conjugated fluorophore, and detected by an Illumina BeadXpress® reader. By using this system, 16 clinically important HPV types (HPV6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68) were correctly genotyped in a multiplex format. The VeraCode‐ASPE genotyping of clinical DNA samples yielded identical results with those obtained by validated PGMY‐reverse blot hybridization assay, providing a new platform for high‐throughput genotyping required for HPV epidemiological surveys.</description><subject>allele-specific primer extension</subject><subject>Alleles</subject><subject>Alphapapillomavirus - classification</subject><subject>Alphapapillomavirus - genetics</subject><subject>Alphapapillomavirus - isolation & purification</subject><subject>DNA Primers - genetics</subject><subject>Female</subject><subject>Genotype</subject><subject>genotyping</subject><subject>human papillomavirus</subject><subject>Humans</subject><subject>multiplex assay</subject><subject>Multiplex Polymerase Chain Reaction - instrumentation</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Papillomavirus Infections - diagnosis</subject><subject>Papillomavirus Infections - virology</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtv1DAURi0EokPhLyDvWCX4GTsSGzSiD6kt4i2xsUx803pw4mAnJfPvSZgya7zxle537rUPQpiSki7n9a6kXOiCCEZLRigtCRGkKudHaHNsPEYbwrUsZEXICXqW844QppgWT9EJY1RURJEN6m7iPQTcTWH0Q4AZHL6FPo77wfe3OLb4bupsjwc7-BBiZ-99mjKe8tq1-Csku40OChsCBCjyAI1vfYOH5DtIGOYR-uxjjzsY76J7jp60NmR48XCfoi9n7z5vL4qr9-eX27dXRSOpqgrpdO2AtqrWVHLpKi2prGjjJGsEqWvGWlpp12gqNJcg2VLUjvLlq7xlgvFT9Oowd0jx1wR5NJ3PDYRge4hTNjXTXDElxJLUh2STYs4JWrM-3aa9ocSsrs3OrErNqtSsrs1f12Ze0JcPS6YfHbgj-E_uEnhzCPz2Afb_PdhcX14vxYIXB9znEeYjbtNPUymupPl2c27OlCKfvn_8YC74H3SSnCk</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Kitamura-Muramatsu, Yuri</creator><creator>Kusumoto-Matsuo, Rika</creator><creator>Kondo, Kazunari</creator><creator>Mori, Seiichiro</creator><creator>Saito, Susumu</creator><creator>Tsukahara, Yusuke</creator><creator>Kukimoto, Iwao</creator><general>Blackwell Publishing Asia</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201202</creationdate><title>Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method</title><author>Kitamura-Muramatsu, Yuri ; Kusumoto-Matsuo, Rika ; Kondo, Kazunari ; Mori, Seiichiro ; Saito, Susumu ; Tsukahara, Yusuke ; Kukimoto, Iwao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5176-5d89de1f7981535d6851561cd52c409922f168dc814835e528149d135603f2423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>allele-specific primer extension</topic><topic>Alleles</topic><topic>Alphapapillomavirus - classification</topic><topic>Alphapapillomavirus - genetics</topic><topic>Alphapapillomavirus - isolation & purification</topic><topic>DNA Primers - genetics</topic><topic>Female</topic><topic>Genotype</topic><topic>genotyping</topic><topic>human papillomavirus</topic><topic>Humans</topic><topic>multiplex assay</topic><topic>Multiplex Polymerase Chain Reaction - instrumentation</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Papillomavirus Infections - diagnosis</topic><topic>Papillomavirus Infections - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitamura-Muramatsu, Yuri</creatorcontrib><creatorcontrib>Kusumoto-Matsuo, Rika</creatorcontrib><creatorcontrib>Kondo, Kazunari</creatorcontrib><creatorcontrib>Mori, Seiichiro</creatorcontrib><creatorcontrib>Saito, Susumu</creatorcontrib><creatorcontrib>Tsukahara, Yusuke</creatorcontrib><creatorcontrib>Kukimoto, Iwao</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitamura-Muramatsu, Yuri</au><au>Kusumoto-Matsuo, Rika</au><au>Kondo, Kazunari</au><au>Mori, Seiichiro</au><au>Saito, Susumu</au><au>Tsukahara, Yusuke</au><au>Kukimoto, Iwao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiol Immunol</addtitle><date>2012-02</date><risdate>2012</risdate><volume>56</volume><issue>2</issue><spage>128</spage><epage>133</epage><pages>128-133</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><abstract>ABSTRACT
A VeraCode‐allele‐specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)‐DNA. Oligonucleotide primers containing HPV‐type‐specific L1 sequences were annealed to HPV‐DNA amplified by PGMY‐PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The labeled DNA was captured by VeraCode beads through hybridization, stained with a streptavidin‐conjugated fluorophore, and detected by an Illumina BeadXpress® reader. By using this system, 16 clinically important HPV types (HPV6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68) were correctly genotyped in a multiplex format. The VeraCode‐ASPE genotyping of clinical DNA samples yielded identical results with those obtained by validated PGMY‐reverse blot hybridization assay, providing a new platform for high‐throughput genotyping required for HPV epidemiological surveys.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>22146070</pmid><doi>10.1111/j.1348-0421.2011.00406.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0385-5600 |
| ispartof | Microbiology and immunology, 2012-02, Vol.56 (2), p.128-133 |
| issn | 0385-5600 1348-0421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_928372744 |
| source | MEDLINE; Wiley Free Content; Open Access Titles of Japan; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | allele-specific primer extension Alleles Alphapapillomavirus - classification Alphapapillomavirus - genetics Alphapapillomavirus - isolation & purification DNA Primers - genetics Female Genotype genotyping human papillomavirus Humans multiplex assay Multiplex Polymerase Chain Reaction - instrumentation Multiplex Polymerase Chain Reaction - methods Papillomavirus Infections - diagnosis Papillomavirus Infections - virology |
| title | Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T00%3A37%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20multiplexed%20genotyping%20of%20human%20papillomavirus%20using%20a%20VeraCode-allele-specific%20primer%20extension%20method&rft.jtitle=Microbiology%20and%20immunology&rft.au=Kitamura-Muramatsu,%20Yuri&rft.date=2012-02&rft.volume=56&rft.issue=2&rft.spage=128&rft.epage=133&rft.pages=128-133&rft.issn=0385-5600&rft.eissn=1348-0421&rft_id=info:doi/10.1111/j.1348-0421.2011.00406.x&rft_dat=%3Cproquest_cross%3E928372744%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=928372744&rft_id=info:pmid/22146070&rfr_iscdi=true |