Mutagenicity of ipriflavone in vivo and in vitro
► Mutagenicity of ipriflavone was evaluated through the micronucleus test. ► Ipriflavone was shown to be mutagenic and cytotoxic in vitro but not in vivo. ► Further studies are needed in order to validate the safety of ipriflavone as medicine. Ipriflavone (7-isopropoxy-isoflavone) is a semisynthetic...
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| Veröffentlicht in: | Food and chemical toxicology 2012-03, Vol.50 (3-4), p.996-1000 |
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| creator | Belcavello, Luciano Vencioneck Dutra, Jean Carlos de Freitas, Josivany Valério Aranha, Ivar Pinheiro Batitucci, Maria do Carmo Pimentel |
| description | ► Mutagenicity of ipriflavone was evaluated through the micronucleus test. ► Ipriflavone was shown to be mutagenic and cytotoxic in vitro but not in vivo. ► Further studies are needed in order to validate the safety of ipriflavone as medicine.
Ipriflavone (7-isopropoxy-isoflavone) is a semisynthetic isoflavone derivative from daidzein and prescribed to prevent and treat osteoporosis in postmenopausal women. In the present study, ipriflavone was investigated with regard to their cytotoxic and mutagenic effects using the micronucleus assay (MN) in vivo on cells of bone marrow and peripheral blood of Swiss albino mice and the micronucleus test with the cytokinesis-blocked micronucleus assay (CBMN assay) on human peripheral blood lymphocytes. The studies were performed in mice with three dosages of the drug, 1.71, 8.57 and 42.85mg/kgbw in single oral exposure, and for two dosages, 5 and 10μg/mL in the CBMN assay. Ipriflavone, in the dosages tested, did not differ from controls neither in the induction of MN nor induced cytotoxicity to cells in the in vivo test. However, in the CBMN assay, the concentration of 10μg/mL induced a statistically significant increase in MN formation and decreased cell proliferation, demonstrating to be mutagenic and cytotoxic at this concentration. |
| doi_str_mv | 10.1016/j.fct.2011.12.013 |
| format | Article |
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Ipriflavone (7-isopropoxy-isoflavone) is a semisynthetic isoflavone derivative from daidzein and prescribed to prevent and treat osteoporosis in postmenopausal women. In the present study, ipriflavone was investigated with regard to their cytotoxic and mutagenic effects using the micronucleus assay (MN) in vivo on cells of bone marrow and peripheral blood of Swiss albino mice and the micronucleus test with the cytokinesis-blocked micronucleus assay (CBMN assay) on human peripheral blood lymphocytes. The studies were performed in mice with three dosages of the drug, 1.71, 8.57 and 42.85mg/kgbw in single oral exposure, and for two dosages, 5 and 10μg/mL in the CBMN assay. Ipriflavone, in the dosages tested, did not differ from controls neither in the induction of MN nor induced cytotoxicity to cells in the in vivo test. However, in the CBMN assay, the concentration of 10μg/mL induced a statistically significant increase in MN formation and decreased cell proliferation, demonstrating to be mutagenic and cytotoxic at this concentration.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2011.12.013</identifier><identifier>PMID: 22200590</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; bone marrow cells ; cell proliferation ; Chemical mutagenesis ; cytotoxicity ; daidzein ; Dose-Response Relationship, Drug ; drugs ; Female ; Human lymphocytes ; Humans ; In Vitro Techniques ; Ipriflavone ; Isoflavones - toxicity ; lymphocytes ; Male ; Medical sciences ; Mice ; Micronucleus assay ; Micronucleus Tests ; Mutagenicity ; Mutagens - toxicity ; oral exposure ; osteoporosis ; postmenopause ; Toxicology ; women</subject><ispartof>Food and chemical toxicology, 2012-03, Vol.50 (3-4), p.996-1000</ispartof><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-935e315f61e91f77a87d8bca5d5c5e5a869ad387cc21a4d48d74bcb379fcd72f3</citedby><cites>FETCH-LOGICAL-c472t-935e315f61e91f77a87d8bca5d5c5e5a869ad387cc21a4d48d74bcb379fcd72f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S027869151100679X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25661290$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22200590$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Belcavello, Luciano</creatorcontrib><creatorcontrib>Vencioneck Dutra, Jean Carlos</creatorcontrib><creatorcontrib>de Freitas, Josivany Valério</creatorcontrib><creatorcontrib>Aranha, Ivar Pinheiro</creatorcontrib><creatorcontrib>Batitucci, Maria do Carmo Pimentel</creatorcontrib><title>Mutagenicity of ipriflavone in vivo and in vitro</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>► Mutagenicity of ipriflavone was evaluated through the micronucleus test. ► Ipriflavone was shown to be mutagenic and cytotoxic in vitro but not in vivo. ► Further studies are needed in order to validate the safety of ipriflavone as medicine.
Ipriflavone (7-isopropoxy-isoflavone) is a semisynthetic isoflavone derivative from daidzein and prescribed to prevent and treat osteoporosis in postmenopausal women. In the present study, ipriflavone was investigated with regard to their cytotoxic and mutagenic effects using the micronucleus assay (MN) in vivo on cells of bone marrow and peripheral blood of Swiss albino mice and the micronucleus test with the cytokinesis-blocked micronucleus assay (CBMN assay) on human peripheral blood lymphocytes. The studies were performed in mice with three dosages of the drug, 1.71, 8.57 and 42.85mg/kgbw in single oral exposure, and for two dosages, 5 and 10μg/mL in the CBMN assay. Ipriflavone, in the dosages tested, did not differ from controls neither in the induction of MN nor induced cytotoxicity to cells in the in vivo test. However, in the CBMN assay, the concentration of 10μg/mL induced a statistically significant increase in MN formation and decreased cell proliferation, demonstrating to be mutagenic and cytotoxic at this concentration.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>bone marrow cells</subject><subject>cell proliferation</subject><subject>Chemical mutagenesis</subject><subject>cytotoxicity</subject><subject>daidzein</subject><subject>Dose-Response Relationship, Drug</subject><subject>drugs</subject><subject>Female</subject><subject>Human lymphocytes</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Ipriflavone</subject><subject>Isoflavones - toxicity</subject><subject>lymphocytes</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Micronucleus assay</subject><subject>Micronucleus Tests</subject><subject>Mutagenicity</subject><subject>Mutagens - toxicity</subject><subject>oral exposure</subject><subject>osteoporosis</subject><subject>postmenopause</subject><subject>Toxicology</subject><subject>women</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PGzEQhi1URELgB_TS7qXitNsZO16v1ROKoEUCcSicLccfkaPNmtqbSPz7GjZtbz3NHJ53Ph5CPiI0CNh-3TbejA0FxAZpA8hOyBw7weqWcfxA5kBFV7cS-Yyc57wFAIGiPSMzSikAlzAn8LAf9cYNwYTxtYq-Ci8p-F4f4uCqMFSHcIiVHuzUjylekFOv--wuj3VBnm9vnlY_6vvH73er6_vaLAUda8m4Y8h9i06iF0J3wnZro7nlhjuuu1ZqyzphDEW9tMvOiuXarJmQ3lhBPVuQq2nuS4q_9i6PaheycX2vBxf3WcnyG2MSWCFxIk2KOSfnVXlhp9OrQlBvntRWFU_qzZNCqoqnkvl0nL5f75z9m_gjpgBfjoDORvc-6cGE_I_jbYv0nfs8cV5HpTepMM8_yyYOgAIlyEJ8mwhXbB2CSyqb4AbjbEiunGVj-M-hvwGlj419</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Belcavello, Luciano</creator><creator>Vencioneck Dutra, Jean Carlos</creator><creator>de Freitas, Josivany Valério</creator><creator>Aranha, Ivar Pinheiro</creator><creator>Batitucci, Maria do Carmo Pimentel</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Mutagenicity of ipriflavone in vivo and in vitro</title><author>Belcavello, Luciano ; Vencioneck Dutra, Jean Carlos ; de Freitas, Josivany Valério ; Aranha, Ivar Pinheiro ; Batitucci, Maria do Carmo Pimentel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-935e315f61e91f77a87d8bca5d5c5e5a869ad387cc21a4d48d74bcb379fcd72f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>bone marrow cells</topic><topic>cell proliferation</topic><topic>Chemical mutagenesis</topic><topic>cytotoxicity</topic><topic>daidzein</topic><topic>Dose-Response Relationship, Drug</topic><topic>drugs</topic><topic>Female</topic><topic>Human lymphocytes</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Ipriflavone</topic><topic>Isoflavones - toxicity</topic><topic>lymphocytes</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Micronucleus assay</topic><topic>Micronucleus Tests</topic><topic>Mutagenicity</topic><topic>Mutagens - toxicity</topic><topic>oral exposure</topic><topic>osteoporosis</topic><topic>postmenopause</topic><topic>Toxicology</topic><topic>women</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belcavello, Luciano</creatorcontrib><creatorcontrib>Vencioneck Dutra, Jean Carlos</creatorcontrib><creatorcontrib>de Freitas, Josivany Valério</creatorcontrib><creatorcontrib>Aranha, Ivar Pinheiro</creatorcontrib><creatorcontrib>Batitucci, Maria do Carmo Pimentel</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belcavello, Luciano</au><au>Vencioneck Dutra, Jean Carlos</au><au>de Freitas, Josivany Valério</au><au>Aranha, Ivar Pinheiro</au><au>Batitucci, Maria do Carmo Pimentel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutagenicity of ipriflavone in vivo and in vitro</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>50</volume><issue>3-4</issue><spage>996</spage><epage>1000</epage><pages>996-1000</pages><issn>0278-6915</issn><eissn>1873-6351</eissn><coden>FCTOD7</coden><abstract>► Mutagenicity of ipriflavone was evaluated through the micronucleus test. ► Ipriflavone was shown to be mutagenic and cytotoxic in vitro but not in vivo. ► Further studies are needed in order to validate the safety of ipriflavone as medicine.
Ipriflavone (7-isopropoxy-isoflavone) is a semisynthetic isoflavone derivative from daidzein and prescribed to prevent and treat osteoporosis in postmenopausal women. In the present study, ipriflavone was investigated with regard to their cytotoxic and mutagenic effects using the micronucleus assay (MN) in vivo on cells of bone marrow and peripheral blood of Swiss albino mice and the micronucleus test with the cytokinesis-blocked micronucleus assay (CBMN assay) on human peripheral blood lymphocytes. The studies were performed in mice with three dosages of the drug, 1.71, 8.57 and 42.85mg/kgbw in single oral exposure, and for two dosages, 5 and 10μg/mL in the CBMN assay. Ipriflavone, in the dosages tested, did not differ from controls neither in the induction of MN nor induced cytotoxicity to cells in the in vivo test. However, in the CBMN assay, the concentration of 10μg/mL induced a statistically significant increase in MN formation and decreased cell proliferation, demonstrating to be mutagenic and cytotoxic at this concentration.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>22200590</pmid><doi>10.1016/j.fct.2011.12.013</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences bone marrow cells cell proliferation Chemical mutagenesis cytotoxicity daidzein Dose-Response Relationship, Drug drugs Female Human lymphocytes Humans In Vitro Techniques Ipriflavone Isoflavones - toxicity lymphocytes Male Medical sciences Mice Micronucleus assay Micronucleus Tests Mutagenicity Mutagens - toxicity oral exposure osteoporosis postmenopause Toxicology women |
| title | Mutagenicity of ipriflavone in vivo and in vitro |
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