Lipase Immobilization on Differently Functionalized Vinyl-Based Amphiphilic Polymers: Influence of Phase Segregation on the Enzyme Hydrolytic Activity

Microbial lipase from Candida rugosa was immobilized by physical adsorption onto an ethylene–vinyl alcohol polymer (EVAL) functionalized with acyl chlorides. To evaluate the influence of the reagent chain-length on the amount and activity of immobilized lipase, three differently long aliphatic fatty...

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Veröffentlicht in:	Biomacromolecules 2012-03, Vol.13 (3), p.805-813
Hauptverfasser:	Bellusci, Mariangela, Francolini, Iolanda, Martinelli, Andrea, D’Ilario, Lucio, Piozzi, Antonella
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences Biological and medical sciences Biotechnology Candida - enzymology Candida rugosa Enzymes, Immobilized - chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydrolysis Hydrophobic and Hydrophilic Interactions Immobilization of enzymes and other molecules Immobilization techniques Kinetics Lipase - chemistry Lipase - metabolism Magnetic Resonance Spectroscopy Methods. Procedures. Technologies Organic polymers Physicochemistry of polymers Polymers - chemistry Polymers - metabolism Properties and characterization Special properties (catalyst, reagent or carrier) Temperature Vinyl Compounds - chemistry
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