Characterization of the ABA signal transduction pathway in Vitis vinifera
[Display omitted] ► The grape ABA signaling cascade consists of at least six SnRK2s. ► Our results indicate organ specificity in the grape ABF genes. ► VvSnRK2.1 and VvSnRK2.6, can serve as the major VvSnRK2 candidates involved in the stomata response to abiotic stress. The plant hormone abscisic ac...
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| Veröffentlicht in: | Plant science (Limerick) 2012-05, Vol.187, p.89-96 |
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| creator | Boneh, Uri Biton, Iris Schwartz, Amnon Ben-Ari, Giora |
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► The grape ABA signaling cascade consists of at least six SnRK2s. ► Our results indicate organ specificity in the grape ABF genes. ► VvSnRK2.1 and VvSnRK2.6, can serve as the major VvSnRK2 candidates involved in the stomata response to abiotic stress.
The plant hormone abscisic acid (ABA) regulates many key processes in plants including the response to abiotic stress. ABA signal transduction consists of a double-negative regulatory mechanism, whereby ABA-bound PYR/RCARs inhibit PP2C activity, and PP2Cs inactivate SnRK2s. We studied and analyzed the various genes participating in the ABA signaling cascade of the grape (Vitis vinifera). The grape ABA signal transduction consists of at least six SnRK2s. Yeast two-hybrid system was used to test direct interactions between core components of grape ABA signal transduction. We found that a total of forty eight interactions can occur between the various components. Exogenous abscisic acid (ABA) and abiotic stresses such as drought, high salt concentration and cold, were applied to vines growing in a hydroponic system. These stresses regulated the expression of various grape SnRK2s as well as ABFs in leaves and roots. Based on the interactions between SnRK2s and its targets and the expression pattern, we suggest that VvSnRK2.1 and VvSnRK2.6, can be considered the major VvSnRK2 candidates involved in the stomata response to abiotic stress. Furthermore, we found that the expression pattern of the two grape ABF genes indicates organ specificity of these genes. The key role of ABA signaling in response to abiotic stresses makes the genes involve in this signaling potential candidates for manipulation in programs designed to improve fruit tree performance in extreme environments. |
| doi_str_mv | 10.1016/j.plantsci.2012.01.015 |
| format | Article |
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► The grape ABA signaling cascade consists of at least six SnRK2s. ► Our results indicate organ specificity in the grape ABF genes. ► VvSnRK2.1 and VvSnRK2.6, can serve as the major VvSnRK2 candidates involved in the stomata response to abiotic stress.
The plant hormone abscisic acid (ABA) regulates many key processes in plants including the response to abiotic stress. ABA signal transduction consists of a double-negative regulatory mechanism, whereby ABA-bound PYR/RCARs inhibit PP2C activity, and PP2Cs inactivate SnRK2s. We studied and analyzed the various genes participating in the ABA signaling cascade of the grape (Vitis vinifera). The grape ABA signal transduction consists of at least six SnRK2s. Yeast two-hybrid system was used to test direct interactions between core components of grape ABA signal transduction. We found that a total of forty eight interactions can occur between the various components. Exogenous abscisic acid (ABA) and abiotic stresses such as drought, high salt concentration and cold, were applied to vines growing in a hydroponic system. These stresses regulated the expression of various grape SnRK2s as well as ABFs in leaves and roots. Based on the interactions between SnRK2s and its targets and the expression pattern, we suggest that VvSnRK2.1 and VvSnRK2.6, can be considered the major VvSnRK2 candidates involved in the stomata response to abiotic stress. Furthermore, we found that the expression pattern of the two grape ABF genes indicates organ specificity of these genes. The key role of ABA signaling in response to abiotic stresses makes the genes involve in this signaling potential candidates for manipulation in programs designed to improve fruit tree performance in extreme environments.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2012.01.015</identifier><identifier>PMID: 22404836</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>abiotic stress ; Abscisic acid ; Abscisic Acid - genetics ; Abscisic Acid - metabolism ; Abscisic Acid - pharmacology ; Adaptation, Physiological - genetics ; Basic-Leucine Zipper Transcription Factors - genetics ; Basic-Leucine Zipper Transcription Factors - metabolism ; Biological and medical sciences ; cold ; drought ; fruit trees ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; genes ; Genes, Plant ; grapes ; hydroponics ; leaves ; Plant Growth Regulators - genetics ; Plant Growth Regulators - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Stomata - physiology ; roots ; salt concentration ; Signal transduction ; Signal Transduction - genetics ; stomata ; Stress, Physiological - genetics ; Two-Hybrid System Techniques ; vines ; Vitis - genetics ; Vitis - metabolism ; Vitis vinifera</subject><ispartof>Plant science (Limerick), 2012-05, Vol.187, p.89-96</ispartof><rights>2012 Elsevier Ireland Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2012 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-eb54e1578fb97ed567ada8fd245360505ce38ac14ba6a4f1a245d5f9e71ca0e93</citedby><cites>FETCH-LOGICAL-c487t-eb54e1578fb97ed567ada8fd245360505ce38ac14ba6a4f1a245d5f9e71ca0e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168945212000258$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25666405$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22404836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boneh, Uri</creatorcontrib><creatorcontrib>Biton, Iris</creatorcontrib><creatorcontrib>Schwartz, Amnon</creatorcontrib><creatorcontrib>Ben-Ari, Giora</creatorcontrib><title>Characterization of the ABA signal transduction pathway in Vitis vinifera</title><title>Plant science (Limerick)</title><addtitle>Plant Sci</addtitle><description>[Display omitted]
► The grape ABA signaling cascade consists of at least six SnRK2s. ► Our results indicate organ specificity in the grape ABF genes. ► VvSnRK2.1 and VvSnRK2.6, can serve as the major VvSnRK2 candidates involved in the stomata response to abiotic stress.
The plant hormone abscisic acid (ABA) regulates many key processes in plants including the response to abiotic stress. ABA signal transduction consists of a double-negative regulatory mechanism, whereby ABA-bound PYR/RCARs inhibit PP2C activity, and PP2Cs inactivate SnRK2s. We studied and analyzed the various genes participating in the ABA signaling cascade of the grape (Vitis vinifera). The grape ABA signal transduction consists of at least six SnRK2s. Yeast two-hybrid system was used to test direct interactions between core components of grape ABA signal transduction. We found that a total of forty eight interactions can occur between the various components. Exogenous abscisic acid (ABA) and abiotic stresses such as drought, high salt concentration and cold, were applied to vines growing in a hydroponic system. These stresses regulated the expression of various grape SnRK2s as well as ABFs in leaves and roots. Based on the interactions between SnRK2s and its targets and the expression pattern, we suggest that VvSnRK2.1 and VvSnRK2.6, can be considered the major VvSnRK2 candidates involved in the stomata response to abiotic stress. Furthermore, we found that the expression pattern of the two grape ABF genes indicates organ specificity of these genes. The key role of ABA signaling in response to abiotic stresses makes the genes involve in this signaling potential candidates for manipulation in programs designed to improve fruit tree performance in extreme environments.</description><subject>abiotic stress</subject><subject>Abscisic acid</subject><subject>Abscisic Acid - genetics</subject><subject>Abscisic Acid - metabolism</subject><subject>Abscisic Acid - pharmacology</subject><subject>Adaptation, Physiological - genetics</subject><subject>Basic-Leucine Zipper Transcription Factors - genetics</subject><subject>Basic-Leucine Zipper Transcription Factors - metabolism</subject><subject>Biological and medical sciences</subject><subject>cold</subject><subject>drought</subject><subject>fruit trees</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>grapes</subject><subject>hydroponics</subject><subject>leaves</subject><subject>Plant Growth Regulators - genetics</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Stomata - physiology</subject><subject>roots</subject><subject>salt concentration</subject><subject>Signal transduction</subject><subject>Signal Transduction - genetics</subject><subject>stomata</subject><subject>Stress, Physiological - genetics</subject><subject>Two-Hybrid System Techniques</subject><subject>vines</subject><subject>Vitis - genetics</subject><subject>Vitis - metabolism</subject><subject>Vitis vinifera</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPGzEQgC1EVVLavwB7QT1tanv92L0RotIiIXEAerUm3jFxtNkNtpOK_nq8JLTHSiP5MN-8PhNyxuiUUaa-raabDvoUrZ9yyviUshzyiExYrauSc9kck0kG67IRkp-QTzGuKKVcSv2RnHAuqKgrNSE38yUEsAmD_wPJD30xuCItsZhdzYron3roihSgj-3WvqU3kJa_4aXwffHLJx-Lne-9wwCfyQcHXcQvh_eUPF5_f5j_LG_vftzMZ7elFbVOJS6kQCZ17RaNxlYqDS3UruVCVopKKi1WNVgmFqBAOAY50UrXoGYWKDbVKfm677sJw_MWYzJrHy12WQcO22garmveNJJlUu1JG4YYAzqzCX4N4cUwakaLZmXeLZrRoqEsh8yFZ4cR28Ua279l79oycHEAIFroXBZkffzHSaWUoGOj8z3nYDDwFDLzeJ8nifEruKbjNZd7ArOyncdg8jLYW2x9QJtMO_j_bfsKj1qdQQ</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Boneh, Uri</creator><creator>Biton, Iris</creator><creator>Schwartz, Amnon</creator><creator>Ben-Ari, Giora</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>Characterization of the ABA signal transduction pathway in Vitis vinifera</title><author>Boneh, Uri ; Biton, Iris ; Schwartz, Amnon ; Ben-Ari, Giora</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-eb54e1578fb97ed567ada8fd245360505ce38ac14ba6a4f1a245d5f9e71ca0e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>abiotic stress</topic><topic>Abscisic acid</topic><topic>Abscisic Acid - genetics</topic><topic>Abscisic Acid - metabolism</topic><topic>Abscisic Acid - pharmacology</topic><topic>Adaptation, Physiological - genetics</topic><topic>Basic-Leucine Zipper Transcription Factors - genetics</topic><topic>Basic-Leucine Zipper Transcription Factors - metabolism</topic><topic>Biological and medical sciences</topic><topic>cold</topic><topic>drought</topic><topic>fruit trees</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>grapes</topic><topic>hydroponics</topic><topic>leaves</topic><topic>Plant Growth Regulators - genetics</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Stomata - physiology</topic><topic>roots</topic><topic>salt concentration</topic><topic>Signal transduction</topic><topic>Signal Transduction - genetics</topic><topic>stomata</topic><topic>Stress, Physiological - genetics</topic><topic>Two-Hybrid System Techniques</topic><topic>vines</topic><topic>Vitis - genetics</topic><topic>Vitis - metabolism</topic><topic>Vitis vinifera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boneh, Uri</creatorcontrib><creatorcontrib>Biton, Iris</creatorcontrib><creatorcontrib>Schwartz, Amnon</creatorcontrib><creatorcontrib>Ben-Ari, Giora</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boneh, Uri</au><au>Biton, Iris</au><au>Schwartz, Amnon</au><au>Ben-Ari, Giora</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the ABA signal transduction pathway in Vitis vinifera</atitle><jtitle>Plant science (Limerick)</jtitle><addtitle>Plant Sci</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>187</volume><spage>89</spage><epage>96</epage><pages>89-96</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>[Display omitted]
► The grape ABA signaling cascade consists of at least six SnRK2s. ► Our results indicate organ specificity in the grape ABF genes. ► VvSnRK2.1 and VvSnRK2.6, can serve as the major VvSnRK2 candidates involved in the stomata response to abiotic stress.
The plant hormone abscisic acid (ABA) regulates many key processes in plants including the response to abiotic stress. ABA signal transduction consists of a double-negative regulatory mechanism, whereby ABA-bound PYR/RCARs inhibit PP2C activity, and PP2Cs inactivate SnRK2s. We studied and analyzed the various genes participating in the ABA signaling cascade of the grape (Vitis vinifera). The grape ABA signal transduction consists of at least six SnRK2s. Yeast two-hybrid system was used to test direct interactions between core components of grape ABA signal transduction. We found that a total of forty eight interactions can occur between the various components. Exogenous abscisic acid (ABA) and abiotic stresses such as drought, high salt concentration and cold, were applied to vines growing in a hydroponic system. These stresses regulated the expression of various grape SnRK2s as well as ABFs in leaves and roots. Based on the interactions between SnRK2s and its targets and the expression pattern, we suggest that VvSnRK2.1 and VvSnRK2.6, can be considered the major VvSnRK2 candidates involved in the stomata response to abiotic stress. Furthermore, we found that the expression pattern of the two grape ABF genes indicates organ specificity of these genes. The key role of ABA signaling in response to abiotic stresses makes the genes involve in this signaling potential candidates for manipulation in programs designed to improve fruit tree performance in extreme environments.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>22404836</pmid><doi>10.1016/j.plantsci.2012.01.015</doi><tpages>8</tpages></addata></record> |
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| subjects | abiotic stress Abscisic acid Abscisic Acid - genetics Abscisic Acid - metabolism Abscisic Acid - pharmacology Adaptation, Physiological - genetics Basic-Leucine Zipper Transcription Factors - genetics Basic-Leucine Zipper Transcription Factors - metabolism Biological and medical sciences cold drought fruit trees Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Profiling Gene Expression Regulation, Plant genes Genes, Plant grapes hydroponics leaves Plant Growth Regulators - genetics Plant Growth Regulators - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plant Stomata - physiology roots salt concentration Signal transduction Signal Transduction - genetics stomata Stress, Physiological - genetics Two-Hybrid System Techniques vines Vitis - genetics Vitis - metabolism Vitis vinifera |
| title | Characterization of the ABA signal transduction pathway in Vitis vinifera |
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