Human cytomegalovirus latency-associated protein LUNA is expressed during HCMV infections in vivo
Human cytomegalovirus (HCMV) latency is poorly understood. We previously described a novel HCMV latency-associated transcript, UL81-82ast, coding for a protein designated LUNA (latency unique natural antigen). The aim of this study was to confirm the presence of LUNA in HCMV-seropositive donors. Sta...
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| Veröffentlicht in: | Archives of virology 2011-10, Vol.156 (10), p.1847-1851 |
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| creator | Bego, Mariana G. Keyes, Lisa R. Maciejewski, Jarek St Jeor, Stephen C. |
| description | Human cytomegalovirus (HCMV) latency is poorly understood. We previously described a novel HCMV latency-associated transcript, UL81-82ast, coding for a protein designated LUNA (latency unique natural antigen). The aim of this study was to confirm the presence of LUNA in HCMV-seropositive donors. Standard co-immunoprecipitation and ELISA assays were used to detect antibodies against the LUNA protein in the sera of HCMV-seropositive donors. Specific antibodies against LUNA were detected in all HCMV-seropositive donors but in none of the seronegative donors. These data confirm that LUNA is expressed during in vivo infections and is capable of eliciting an immune response. |
| doi_str_mv | 10.1007/s00705-011-1027-7 |
| format | Article |
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We previously described a novel HCMV latency-associated transcript, UL81-82ast, coding for a protein designated LUNA (latency unique natural antigen). The aim of this study was to confirm the presence of LUNA in HCMV-seropositive donors. Standard co-immunoprecipitation and ELISA assays were used to detect antibodies against the LUNA protein in the sera of HCMV-seropositive donors. Specific antibodies against LUNA were detected in all HCMV-seropositive donors but in none of the seronegative donors. These data confirm that LUNA is expressed during in vivo infections and is capable of eliciting an immune response.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-011-1027-7</identifier><identifier>PMID: 21625978</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Antibodies ; Antibodies, Viral - immunology ; Antigens ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Brief Report ; Cytomegalovirus ; Cytomegalovirus - genetics ; Cytomegalovirus - immunology ; Cytomegalovirus - physiology ; Cytomegalovirus Infections - immunology ; Cytomegalovirus Infections - virology ; Dendritic cells ; Fundamental and applied biological sciences. 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We previously described a novel HCMV latency-associated transcript, UL81-82ast, coding for a protein designated LUNA (latency unique natural antigen). The aim of this study was to confirm the presence of LUNA in HCMV-seropositive donors. Standard co-immunoprecipitation and ELISA assays were used to detect antibodies against the LUNA protein in the sera of HCMV-seropositive donors. Specific antibodies against LUNA were detected in all HCMV-seropositive donors but in none of the seronegative donors. These data confirm that LUNA is expressed during in vivo infections and is capable of eliciting an immune response.</description><subject>Antibodies</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brief Report</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - genetics</subject><subject>Cytomegalovirus - immunology</subject><subject>Cytomegalovirus - physiology</subject><subject>Cytomegalovirus Infections - immunology</subject><subject>Cytomegalovirus Infections - virology</subject><subject>Dendritic cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Viral</subject><subject>Human cytomegalovirus</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious Diseases</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Proteins</subject><subject>Serology</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><subject>Virology</subject><subject>Virus Latency</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkUtv1DAURi1ERaeFH8AGRUiIVcCP-LWsRsAgDe2Gso0c56ZylcSDbzJi_j1OZ2glJMTGz3OvP-sQ8prRD4xS_RHzQGVJGSsZ5brUz8iKVYKXRlvznKyooFVpFDXn5ALxntJ8IOQLcs6Z4tJqsyJuMw9uLPxhigPcuT7uQ5qx6N0Eoz-UDjH6kDdtsUtxgjAW29vrqyJgAb92CRDzTTunMN4Vm_W3H0UYO_BTiCPmZbEP-_iSnHWuR3h1mi_J7edP39ebcnvz5ev6alv6nHgqNQMhuauUU431tjEVCNeCAiNEyyRo1eiuq4xyQphKSs91p612CrrONC0Xl-T9sW8O-nMGnOohoIe-dyPEGWvLlaVaCv1f0tjKMKulzOTbv8j7OKcxf2OBBKWMqwyxI-RTREzQ1bsUBpcONaP14qk-eqqzp3rxVC8R3pwaz80A7WPFHzEZeHcCHHrXd8mNPuATV0lt2ENCfuRwt0iA9JTw36__BpVLqjc</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Bego, Mariana G.</creator><creator>Keyes, Lisa R.</creator><creator>Maciejewski, Jarek</creator><creator>St Jeor, Stephen C.</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20111001</creationdate><title>Human cytomegalovirus latency-associated protein LUNA is expressed during HCMV infections in vivo</title><author>Bego, Mariana G. ; Keyes, Lisa R. ; Maciejewski, Jarek ; St Jeor, Stephen C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-71e352a46a6b9c9b84e3ade6e833d15e76b7ff486a338455c27f797a6eff8bd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antibodies</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brief Report</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus - genetics</topic><topic>Cytomegalovirus - immunology</topic><topic>Cytomegalovirus - physiology</topic><topic>Cytomegalovirus Infections - immunology</topic><topic>Cytomegalovirus Infections - virology</topic><topic>Dendritic cells</topic><topic>Fundamental and applied biological sciences. 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We previously described a novel HCMV latency-associated transcript, UL81-82ast, coding for a protein designated LUNA (latency unique natural antigen). The aim of this study was to confirm the presence of LUNA in HCMV-seropositive donors. Standard co-immunoprecipitation and ELISA assays were used to detect antibodies against the LUNA protein in the sera of HCMV-seropositive donors. Specific antibodies against LUNA were detected in all HCMV-seropositive donors but in none of the seronegative donors. These data confirm that LUNA is expressed during in vivo infections and is capable of eliciting an immune response.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>21625978</pmid><doi>10.1007/s00705-011-1027-7</doi><tpages>5</tpages></addata></record> |
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| subjects | Antibodies Antibodies, Viral - immunology Antigens Biological and medical sciences Biomedical and Life Sciences Biomedicine Brief Report Cytomegalovirus Cytomegalovirus - genetics Cytomegalovirus - immunology Cytomegalovirus - physiology Cytomegalovirus Infections - immunology Cytomegalovirus Infections - virology Dendritic cells Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Human cytomegalovirus Humans Infections Infectious Diseases Medical Microbiology Microbiology Miscellaneous Proteins Serology Viral Proteins - genetics Viral Proteins - immunology Virology Virus Latency |
| title | Human cytomegalovirus latency-associated protein LUNA is expressed during HCMV infections in vivo |
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