Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo
Exposure to fluoride can induce low sperm quality; however, little is known about the molecular mechanisms by which fluoride exerts its toxic effects. This study was conducted to evaluate ultrastructure, oxidative stress, and apoptosis in sperm of mice treated with 150 mg/l NaF for 49 days. Furtherm...
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| Veröffentlicht in: | Archives of toxicology 2011-11, Vol.85 (11), p.1441-1452 |
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| creator | Sun, Zilong Niu, Ruiyan Wang, Bin Jiao, Zhibin Wang, Jinming Zhang, Jianhai Wang, Shaolin Wang, Jundong |
| description | Exposure to fluoride can induce low sperm quality; however, little is known about the molecular mechanisms by which fluoride exerts its toxic effects. This study was conducted to evaluate ultrastructure, oxidative stress, and apoptosis in sperm of mice treated with 150 mg/l NaF for 49 days. Furthermore, microarray analysis was also utilized to characterize the effects of fluoride in gene expression profiling on mice sperm. An increased ROS and a decreased TAC accompanied with distinct morphological changes and significant apoptosis were observed in mice sperm from the fluoride group. Fluoride exposure also significantly elevated the protein expressions of cytochrome c and active caspase-3. In global gene expression profiling, 34 up-regulated and 63 down-regulated genes, which are involved in several sperm biological processes including signal transduction, oxidative stress, apoptosis, electron transport, glycolysis, chemotaxis, spermatogenesis, and sperm capacitation, were significantly differentially expressed. Based on these findings, it was proposed that oxidative stress induced by excessive ROS may trigger sperm apoptosis through mitochondrial impairment, resulting in decreased fertility in mice exposed to fluoride. Microarray analysis also provided several important biological clues for further investigating fluoride-induced damage in sperm morphology and functions. |
| doi_str_mv | 10.1007/s00204-011-0672-7 |
| format | Article |
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This study was conducted to evaluate ultrastructure, oxidative stress, and apoptosis in sperm of mice treated with 150 mg/l NaF for 49 days. Furthermore, microarray analysis was also utilized to characterize the effects of fluoride in gene expression profiling on mice sperm. An increased ROS and a decreased TAC accompanied with distinct morphological changes and significant apoptosis were observed in mice sperm from the fluoride group. Fluoride exposure also significantly elevated the protein expressions of cytochrome c and active caspase-3. In global gene expression profiling, 34 up-regulated and 63 down-regulated genes, which are involved in several sperm biological processes including signal transduction, oxidative stress, apoptosis, electron transport, glycolysis, chemotaxis, spermatogenesis, and sperm capacitation, were significantly differentially expressed. Based on these findings, it was proposed that oxidative stress induced by excessive ROS may trigger sperm apoptosis through mitochondrial impairment, resulting in decreased fertility in mice exposed to fluoride. Microarray analysis also provided several important biological clues for further investigating fluoride-induced damage in sperm morphology and functions.</description><identifier>ISSN: 0340-5761</identifier><identifier>EISSN: 1432-0738</identifier><identifier>DOI: 10.1007/s00204-011-0672-7</identifier><identifier>PMID: 21340527</identifier><identifier>CODEN: ARTODN</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Antioxidants - analysis ; Apoptosis ; Apoptosis - drug effects ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Caspase 3 - genetics ; Caspase 3 - metabolism ; Cytochromes c - genetics ; Cytochromes c - metabolism ; Dose-Response Relationship, Drug ; Down-Regulation ; Environmental Health ; Fluorides ; Gene expression ; Gene Expression Profiling ; Male ; Medical sciences ; Mice ; Microarray Analysis ; Microscopy, Electron, Transmission ; Mitochondria - genetics ; Mitochondria - metabolism ; Occupational Medicine/Industrial Medicine ; Oxidative Stress - drug effects ; Pharmacology/Toxicology ; Reactive Oxygen Species - analysis ; Reproductive Toxicology ; Rodents ; Signal Transduction ; Sodium Fluoride - toxicity ; Sperm ; Spermatogenesis ; Spermatozoa - drug effects ; Toxicity ; Toxicology ; Up-Regulation</subject><ispartof>Archives of toxicology, 2011-11, Vol.85 (11), p.1441-1452</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-83cb11c083c30eb33f0bbb7181d8c5a0c5b344ffee8995f50445331296cae4da3</citedby><cites>FETCH-LOGICAL-c498t-83cb11c083c30eb33f0bbb7181d8c5a0c5b344ffee8995f50445331296cae4da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00204-011-0672-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00204-011-0672-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24703484$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21340527$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Zilong</creatorcontrib><creatorcontrib>Niu, Ruiyan</creatorcontrib><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Jiao, Zhibin</creatorcontrib><creatorcontrib>Wang, Jinming</creatorcontrib><creatorcontrib>Zhang, Jianhai</creatorcontrib><creatorcontrib>Wang, Shaolin</creatorcontrib><creatorcontrib>Wang, Jundong</creatorcontrib><title>Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo</title><title>Archives of toxicology</title><addtitle>Arch Toxicol</addtitle><addtitle>Arch Toxicol</addtitle><description>Exposure to fluoride can induce low sperm quality; however, little is known about the molecular mechanisms by which fluoride exerts its toxic effects. This study was conducted to evaluate ultrastructure, oxidative stress, and apoptosis in sperm of mice treated with 150 mg/l NaF for 49 days. Furthermore, microarray analysis was also utilized to characterize the effects of fluoride in gene expression profiling on mice sperm. An increased ROS and a decreased TAC accompanied with distinct morphological changes and significant apoptosis were observed in mice sperm from the fluoride group. Fluoride exposure also significantly elevated the protein expressions of cytochrome c and active caspase-3. In global gene expression profiling, 34 up-regulated and 63 down-regulated genes, which are involved in several sperm biological processes including signal transduction, oxidative stress, apoptosis, electron transport, glycolysis, chemotaxis, spermatogenesis, and sperm capacitation, were significantly differentially expressed. Based on these findings, it was proposed that oxidative stress induced by excessive ROS may trigger sperm apoptosis through mitochondrial impairment, resulting in decreased fertility in mice exposed to fluoride. Microarray analysis also provided several important biological clues for further investigating fluoride-induced damage in sperm morphology and functions.</description><subject>Animals</subject><subject>Antioxidants - analysis</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Caspase 3 - genetics</subject><subject>Caspase 3 - metabolism</subject><subject>Cytochromes c - genetics</subject><subject>Cytochromes c - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Down-Regulation</subject><subject>Environmental Health</subject><subject>Fluorides</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microarray Analysis</subject><subject>Microscopy, Electron, Transmission</subject><subject>Mitochondria - genetics</subject><subject>Mitochondria - metabolism</subject><subject>Occupational Medicine/Industrial Medicine</subject><subject>Oxidative Stress - drug effects</subject><subject>Pharmacology/Toxicology</subject><subject>Reactive Oxygen Species - analysis</subject><subject>Reproductive Toxicology</subject><subject>Rodents</subject><subject>Signal Transduction</subject><subject>Sodium Fluoride - toxicity</subject><subject>Sperm</subject><subject>Spermatogenesis</subject><subject>Spermatozoa - drug effects</subject><subject>Toxicity</subject><subject>Toxicology</subject><subject>Up-Regulation</subject><issn>0340-5761</issn><issn>1432-0738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kd9rFDEQx4NY7Fn9A3yRRRCfUie_LsmjFKtCsS_6HLLZ2SNlN1mT22L_--a404Lg0xDmM5MvnyHkDYNLBqA_VgAOkgJjFLaaU_2MbJgUnIIW5jnZgJBAld6yc_Ky1jsAxo0VL8g5Z62juN6Q79fTmksckMY0rAGHzi952ecaa-fT0O0wYYe_l4K1xpy6peQxTjHtupi6OQbs6oJlPrzu431-Rc5GP1V8faoX5Of15x9XX-nN7ZdvV59uaJDW7KkRoWcsQKsCsBdihL7vNTNsMEF5CKoXUo4jorFWjQqkVEIwbrfBoxy8uCAfjntbnl8r1r2bYw04TT5hXquzfGtBKisa-e4f8i6vJbVwzgIYa5SBBrEjFEquteDolhJnXx4cA3dQ7Y6qXVPtDqqdbjNvT4vXfsbh78Qftw14fwJ8DX4ai08h1idO6nYfIxvHj1xtrbTD8pTw_78_Ak_ilV8</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Sun, Zilong</creator><creator>Niu, Ruiyan</creator><creator>Wang, Bin</creator><creator>Jiao, Zhibin</creator><creator>Wang, Jinming</creator><creator>Zhang, Jianhai</creator><creator>Wang, Shaolin</creator><creator>Wang, Jundong</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T2</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>MBDVC</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20111101</creationdate><title>Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo</title><author>Sun, Zilong ; Niu, Ruiyan ; Wang, Bin ; Jiao, Zhibin ; Wang, Jinming ; Zhang, Jianhai ; Wang, Shaolin ; Wang, Jundong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-83cb11c083c30eb33f0bbb7181d8c5a0c5b344ffee8995f50445331296cae4da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antioxidants - 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This study was conducted to evaluate ultrastructure, oxidative stress, and apoptosis in sperm of mice treated with 150 mg/l NaF for 49 days. Furthermore, microarray analysis was also utilized to characterize the effects of fluoride in gene expression profiling on mice sperm. An increased ROS and a decreased TAC accompanied with distinct morphological changes and significant apoptosis were observed in mice sperm from the fluoride group. Fluoride exposure also significantly elevated the protein expressions of cytochrome c and active caspase-3. In global gene expression profiling, 34 up-regulated and 63 down-regulated genes, which are involved in several sperm biological processes including signal transduction, oxidative stress, apoptosis, electron transport, glycolysis, chemotaxis, spermatogenesis, and sperm capacitation, were significantly differentially expressed. Based on these findings, it was proposed that oxidative stress induced by excessive ROS may trigger sperm apoptosis through mitochondrial impairment, resulting in decreased fertility in mice exposed to fluoride. Microarray analysis also provided several important biological clues for further investigating fluoride-induced damage in sperm morphology and functions.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21340527</pmid><doi>10.1007/s00204-011-0672-7</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Antioxidants - analysis Apoptosis Apoptosis - drug effects Biological and medical sciences Biomedical and Life Sciences Biomedicine Caspase 3 - genetics Caspase 3 - metabolism Cytochromes c - genetics Cytochromes c - metabolism Dose-Response Relationship, Drug Down-Regulation Environmental Health Fluorides Gene expression Gene Expression Profiling Male Medical sciences Mice Microarray Analysis Microscopy, Electron, Transmission Mitochondria - genetics Mitochondria - metabolism Occupational Medicine/Industrial Medicine Oxidative Stress - drug effects Pharmacology/Toxicology Reactive Oxygen Species - analysis Reproductive Toxicology Rodents Signal Transduction Sodium Fluoride - toxicity Sperm Spermatogenesis Spermatozoa - drug effects Toxicity Toxicology Up-Regulation |
| title | Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo |
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