Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells
► Retroviral overexpression of the active form of LUMAN in D2SC/1 cell line. ► Analysis of differentially expressed genes using microarray. ► Identification and validation of ApoA4 gene as a target of LUMAN in dendritic cells. Dendritic cells (DCs) are professional antigen presenting cells of the im...
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| Veröffentlicht in: | Molecular immunology 2012-02, Vol.50 (1-2), p.66-73 |
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| creator | Sanecka, Anna Ansems, Marleen van Hout-Kuijer, Maaike A. Looman, Maaike W.G. Prosser, Amy C. Welten, Suzanne Gilissen, Christian Sama, Iziah E. Huynen, Martijn A. Veltman, Joris A. Jansen, Bastiaan J.H. Eleveld-Trancikova, Dagmar Adema, Gosse J. |
| description | ► Retroviral overexpression of the active form of LUMAN in D2SC/1 cell line. ► Analysis of differentially expressed genes using microarray. ► Identification and validation of ApoA4 gene as a target of LUMAN in dendritic cells.
Dendritic cells (DCs) are professional antigen presenting cells of the immune system that play a crucial role in initiating immune responses and maintaining self tolerance. Better understanding of the molecular basis of DC immunobiology is required to improve DC-based immunotherapies. We previously described the interaction of transcription factor LUMAN (also known as CREB3 or LZIP) with the DC-specific transmembrane protein DC-STAMP in DCs. Target genes of LUMAN and its role in DCs are currently unknown. In this study we set out to identify genes regulated by LUMAN in DCs using microarray analysis. Expression of a constitutively active form of LUMAN in mouse DC cell line D2SC/1 identified Apolipoprotein A4 (ApoA4) as its target gene. Subsequent validation experiments, bioinformatics-based promoter analysis, and silencing studies confirmed that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs). |
| doi_str_mv | 10.1016/j.molimm.2011.12.003 |
| format | Article |
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Dendritic cells (DCs) are professional antigen presenting cells of the immune system that play a crucial role in initiating immune responses and maintaining self tolerance. Better understanding of the molecular basis of DC immunobiology is required to improve DC-based immunotherapies. We previously described the interaction of transcription factor LUMAN (also known as CREB3 or LZIP) with the DC-specific transmembrane protein DC-STAMP in DCs. Target genes of LUMAN and its role in DCs are currently unknown. In this study we set out to identify genes regulated by LUMAN in DCs using microarray analysis. Expression of a constitutively active form of LUMAN in mouse DC cell line D2SC/1 identified Apolipoprotein A4 (ApoA4) as its target gene. Subsequent validation experiments, bioinformatics-based promoter analysis, and silencing studies confirmed that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs).</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/j.molimm.2011.12.003</identifier><identifier>PMID: 22209087</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antigen-presenting cells ; Apolipoproteins A - genetics ; Bone marrow ; Bone Marrow Cells - drug effects ; Bone Marrow Cells - metabolism ; Brefeldin A - pharmacology ; Cell Line ; Cells, Cultured ; Cyclic AMP Response Element-Binding Protein - genetics ; DC-STAMP protein ; Dendritic cells ; Dendritic Cells - drug effects ; Dendritic Cells - metabolism ; DNA microarrays ; Endoplasmic reticulum ; Female ; Gene expression microarray ; Gene Expression Profiling ; Gene Expression Regulation ; HEK293 Cells ; Humans ; Immune system ; Immunological tolerance ; Immunotherapy ; Membrane proteins ; Mice ; Mice, Inbred C57BL ; NIH 3T3 Cells ; Oligonucleotide Array Sequence Analysis ; Promoters ; Protein Synthesis Inhibitors - pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA Interference ; Self ; Transcription factors ; Transcription regulation</subject><ispartof>Molecular immunology, 2012-02, Vol.50 (1-2), p.66-73</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-ee4ae23efbafffd615d71ee2de5db6ce75dc024220ea4f8fd49a92d4282f44953</citedby><cites>FETCH-LOGICAL-c393t-ee4ae23efbafffd615d71ee2de5db6ce75dc024220ea4f8fd49a92d4282f44953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0161589011008261$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27902,27903,65308</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22209087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sanecka, Anna</creatorcontrib><creatorcontrib>Ansems, Marleen</creatorcontrib><creatorcontrib>van Hout-Kuijer, Maaike A.</creatorcontrib><creatorcontrib>Looman, Maaike W.G.</creatorcontrib><creatorcontrib>Prosser, Amy C.</creatorcontrib><creatorcontrib>Welten, Suzanne</creatorcontrib><creatorcontrib>Gilissen, Christian</creatorcontrib><creatorcontrib>Sama, Iziah E.</creatorcontrib><creatorcontrib>Huynen, Martijn A.</creatorcontrib><creatorcontrib>Veltman, Joris A.</creatorcontrib><creatorcontrib>Jansen, Bastiaan J.H.</creatorcontrib><creatorcontrib>Eleveld-Trancikova, Dagmar</creatorcontrib><creatorcontrib>Adema, Gosse J.</creatorcontrib><title>Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells</title><title>Molecular immunology</title><addtitle>Mol Immunol</addtitle><description>► Retroviral overexpression of the active form of LUMAN in D2SC/1 cell line. ► Analysis of differentially expressed genes using microarray. ► Identification and validation of ApoA4 gene as a target of LUMAN in dendritic cells.
Dendritic cells (DCs) are professional antigen presenting cells of the immune system that play a crucial role in initiating immune responses and maintaining self tolerance. Better understanding of the molecular basis of DC immunobiology is required to improve DC-based immunotherapies. We previously described the interaction of transcription factor LUMAN (also known as CREB3 or LZIP) with the DC-specific transmembrane protein DC-STAMP in DCs. Target genes of LUMAN and its role in DCs are currently unknown. In this study we set out to identify genes regulated by LUMAN in DCs using microarray analysis. Expression of a constitutively active form of LUMAN in mouse DC cell line D2SC/1 identified Apolipoprotein A4 (ApoA4) as its target gene. Subsequent validation experiments, bioinformatics-based promoter analysis, and silencing studies confirmed that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs).</description><subject>Animals</subject><subject>Antigen-presenting cells</subject><subject>Apolipoproteins A - genetics</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Brefeldin A - pharmacology</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Cyclic AMP Response Element-Binding Protein - genetics</subject><subject>DC-STAMP protein</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - metabolism</subject><subject>DNA microarrays</subject><subject>Endoplasmic reticulum</subject><subject>Female</subject><subject>Gene expression microarray</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Immune system</subject><subject>Immunological tolerance</subject><subject>Immunotherapy</subject><subject>Membrane proteins</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>NIH 3T3 Cells</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Promoters</subject><subject>Protein Synthesis Inhibitors - pharmacology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Interference</subject><subject>Self</subject><subject>Transcription factors</subject><subject>Transcription regulation</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtPGzEURq2qqAToP6iQd13NYN_xPLypNEK0IAXYwNpy7OvU0cw4tR2k_HsmDXRZsbqb893XIeQbZyVnvLnalGMY_DiWwDgvOZSMVZ_IgnctFJIL-EwWM8aLupPslJyltGGMNaypv5BTAGCSde2CxH7Swz75RIOja5ww0Yjr3aAzWrra0_wbaY56Sib6bfZhok6bHCJdPt_3D9RbnLJ3fo7129ALqhPVNOu4xvy3HfUTnRkbffaGGhyGdEFOnB4Sfn2r5-T5583T9W2xfPx1d90vC1PJKheIQiNU6FbaOWcbXtuWI4LF2q4ag21tDQMxX4JauM5ZIbUEK6ADJ4Ssq3Py_dh3G8OfHaasRp8OG-gJwy4pCY1kFYfmAyQAb2t5IMWRNDGkFNGpbfSjjnvFmTpoURt11KIOWhQHNWuZY5dvA3arEe2_0LuHGfhxBHB-yIvHqJLxOBm0PqLJygb__wmv8d-hbQ</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Sanecka, Anna</creator><creator>Ansems, Marleen</creator><creator>van Hout-Kuijer, Maaike A.</creator><creator>Looman, Maaike W.G.</creator><creator>Prosser, Amy C.</creator><creator>Welten, Suzanne</creator><creator>Gilissen, Christian</creator><creator>Sama, Iziah E.</creator><creator>Huynen, Martijn A.</creator><creator>Veltman, Joris A.</creator><creator>Jansen, Bastiaan J.H.</creator><creator>Eleveld-Trancikova, Dagmar</creator><creator>Adema, Gosse J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201202</creationdate><title>Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells</title><author>Sanecka, Anna ; Ansems, Marleen ; van Hout-Kuijer, Maaike A. ; Looman, Maaike W.G. ; Prosser, Amy C. ; Welten, Suzanne ; Gilissen, Christian ; Sama, Iziah E. ; Huynen, Martijn A. ; Veltman, Joris A. ; Jansen, Bastiaan J.H. ; Eleveld-Trancikova, Dagmar ; Adema, Gosse J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-ee4ae23efbafffd615d71ee2de5db6ce75dc024220ea4f8fd49a92d4282f44953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antigen-presenting cells</topic><topic>Apolipoproteins A - genetics</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Brefeldin A - pharmacology</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Cyclic AMP Response Element-Binding Protein - genetics</topic><topic>DC-STAMP protein</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - metabolism</topic><topic>DNA microarrays</topic><topic>Endoplasmic reticulum</topic><topic>Female</topic><topic>Gene expression microarray</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Immune system</topic><topic>Immunological tolerance</topic><topic>Immunotherapy</topic><topic>Membrane proteins</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>NIH 3T3 Cells</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Promoters</topic><topic>Protein Synthesis Inhibitors - pharmacology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA Interference</topic><topic>Self</topic><topic>Transcription factors</topic><topic>Transcription regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanecka, Anna</creatorcontrib><creatorcontrib>Ansems, Marleen</creatorcontrib><creatorcontrib>van Hout-Kuijer, Maaike A.</creatorcontrib><creatorcontrib>Looman, Maaike W.G.</creatorcontrib><creatorcontrib>Prosser, Amy C.</creatorcontrib><creatorcontrib>Welten, Suzanne</creatorcontrib><creatorcontrib>Gilissen, Christian</creatorcontrib><creatorcontrib>Sama, Iziah E.</creatorcontrib><creatorcontrib>Huynen, Martijn A.</creatorcontrib><creatorcontrib>Veltman, Joris A.</creatorcontrib><creatorcontrib>Jansen, Bastiaan J.H.</creatorcontrib><creatorcontrib>Eleveld-Trancikova, Dagmar</creatorcontrib><creatorcontrib>Adema, Gosse J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanecka, Anna</au><au>Ansems, Marleen</au><au>van Hout-Kuijer, Maaike A.</au><au>Looman, Maaike W.G.</au><au>Prosser, Amy C.</au><au>Welten, Suzanne</au><au>Gilissen, Christian</au><au>Sama, Iziah E.</au><au>Huynen, Martijn A.</au><au>Veltman, Joris A.</au><au>Jansen, Bastiaan J.H.</au><au>Eleveld-Trancikova, Dagmar</au><au>Adema, Gosse J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>2012-02</date><risdate>2012</risdate><volume>50</volume><issue>1-2</issue><spage>66</spage><epage>73</epage><pages>66-73</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><abstract>► Retroviral overexpression of the active form of LUMAN in D2SC/1 cell line. ► Analysis of differentially expressed genes using microarray. ► Identification and validation of ApoA4 gene as a target of LUMAN in dendritic cells.
Dendritic cells (DCs) are professional antigen presenting cells of the immune system that play a crucial role in initiating immune responses and maintaining self tolerance. Better understanding of the molecular basis of DC immunobiology is required to improve DC-based immunotherapies. We previously described the interaction of transcription factor LUMAN (also known as CREB3 or LZIP) with the DC-specific transmembrane protein DC-STAMP in DCs. Target genes of LUMAN and its role in DCs are currently unknown. In this study we set out to identify genes regulated by LUMAN in DCs using microarray analysis. Expression of a constitutively active form of LUMAN in mouse DC cell line D2SC/1 identified Apolipoprotein A4 (ApoA4) as its target gene. Subsequent validation experiments, bioinformatics-based promoter analysis, and silencing studies confirmed that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs).</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22209087</pmid><doi>10.1016/j.molimm.2011.12.003</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antigen-presenting cells Apolipoproteins A - genetics Bone marrow Bone Marrow Cells - drug effects Bone Marrow Cells - metabolism Brefeldin A - pharmacology Cell Line Cells, Cultured Cyclic AMP Response Element-Binding Protein - genetics DC-STAMP protein Dendritic cells Dendritic Cells - drug effects Dendritic Cells - metabolism DNA microarrays Endoplasmic reticulum Female Gene expression microarray Gene Expression Profiling Gene Expression Regulation HEK293 Cells Humans Immune system Immunological tolerance Immunotherapy Membrane proteins Mice Mice, Inbred C57BL NIH 3T3 Cells Oligonucleotide Array Sequence Analysis Promoters Protein Synthesis Inhibitors - pharmacology Reverse Transcriptase Polymerase Chain Reaction RNA Interference Self Transcription factors Transcription regulation |
| title | Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells |
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