Sharp decrease of genetic variation in two Spanish localities of razor clam Ensis siliqua: natural fluctuation or Prestige oil spill effects?
Pollution is one of the main concerns in marine ecosystems nowadays. Environmental anthropogenic-mediated toxicants may affect genetic diversity both at the individual and ecosystem levels and may also alter the genetic structure of populations. This study examined the temporal pattern of genetic di...
Gespeichert in:
| Veröffentlicht in: | Ecotoxicology (London) 2012, Vol.21 (1), p.225-233 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 233 |
|---|---|
| container_issue | 1 |
| container_start_page | 225 |
| container_title | Ecotoxicology (London) |
| container_volume | 21 |
| creator | Fernandez-Tajes, J Arias-Perez, A Fernandez-Moreno, M Mendez, J |
| description | Pollution is one of the main concerns in marine ecosystems nowadays. Environmental anthropogenic-mediated toxicants may affect genetic diversity both at the individual and ecosystem levels and may also alter the genetic structure of populations. This study examined the temporal pattern of genetic diversity among populations of the benthic bivalve
Ensis siliqua
in two locations of Galicia, following the Prestige oil spillage. On November 13, 2002 the oil tanker Prestige sank at 240 km from Galician coast and 63,000 tonnes of heavy fuel were released to the marine environment.
E. siliqua
samples were sampled between 2001 and 2006. Genetic variation was assessed by means of Random Amplification of Polymorphic DNA (RAPD). A significant decrease in genetic diversity was observed for the 2006 samples. Nei’s genetic distance, fixation index (PhiPT), and PCA values also supported differences in the 2006 samples. We hypothesize that the temporal genetic variation observed in
E. siliqua
populations is due to a strong effect of genetic drift caused by a reduction in population size and that the indirect effects of the Prestige spill possibly caused this reduction. |
| doi_str_mv | 10.1007/s10646-011-0781-3 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_926888065</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A713748769</galeid><sourcerecordid>A713748769</sourcerecordid><originalsourceid>FETCH-LOGICAL-c441t-ffb5d5c3ec9861e4af402c2fd1ca5da13ac52f5e78d0bfac6f48e8e1246456143</originalsourceid><addsrcrecordid>eNp1kUFrFTEUhQdR7LP6A9xIcONqapLJTDJupJRWhYJCdR3uy9y8pmSS1yRT0f_gf24eUxUEyeJC8p2Twz1N85LRE0apfJsZHcTQUsZaKhVru0fNhvWyazvK5ONmQ8eha0c-8qPmWc43lNJRCvq0OeJMScm53DS_rq4h7cmEJiFkJNGSHQYszpA7SA6Ki4G4QMr3SK72EFy-Jj4a8K44zAc8wc-YiPEwk_OQXSbZeXe7wDsSoCwJPLF-MWVZrSr6JWEublf_cp7kvfOeoLVoSn7_vHliwWd88TCPm28X51_PPraXnz98Oju9bI0QrLTWbvupNx2aUQ0MBVhBueF2Ygb6CVgHpue2R6kmurVgBisUKmRcDKIfmOiOmzer7z7F26XG0bPLBr2HgHHJeuSDUooOfSVf_0PexCWFGk6P1YjWbasKnazQDjxqF2wsCUw9E87OxIDW1ftTyToplBzGKmCrwKSYc0Kr98nNkH5oRvWhWr1Wq2u1-lCt7qrm1UOSZTvj9Efxu8sK8BXI9SnsMP2N-n_Xe5UqsMc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>914305738</pqid></control><display><type>article</type><title>Sharp decrease of genetic variation in two Spanish localities of razor clam Ensis siliqua: natural fluctuation or Prestige oil spill effects?</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Fernandez-Tajes, J ; Arias-Perez, A ; Fernandez-Moreno, M ; Mendez, J</creator><creatorcontrib>Fernandez-Tajes, J ; Arias-Perez, A ; Fernandez-Moreno, M ; Mendez, J</creatorcontrib><description>Pollution is one of the main concerns in marine ecosystems nowadays. Environmental anthropogenic-mediated toxicants may affect genetic diversity both at the individual and ecosystem levels and may also alter the genetic structure of populations. This study examined the temporal pattern of genetic diversity among populations of the benthic bivalve
Ensis siliqua
in two locations of Galicia, following the Prestige oil spillage. On November 13, 2002 the oil tanker Prestige sank at 240 km from Galician coast and 63,000 tonnes of heavy fuel were released to the marine environment.
E. siliqua
samples were sampled between 2001 and 2006. Genetic variation was assessed by means of Random Amplification of Polymorphic DNA (RAPD). A significant decrease in genetic diversity was observed for the 2006 samples. Nei’s genetic distance, fixation index (PhiPT), and PCA values also supported differences in the 2006 samples. We hypothesize that the temporal genetic variation observed in
E. siliqua
populations is due to a strong effect of genetic drift caused by a reduction in population size and that the indirect effects of the Prestige spill possibly caused this reduction.</description><identifier>ISSN: 0963-9292</identifier><identifier>EISSN: 1573-3017</identifier><identifier>DOI: 10.1007/s10646-011-0781-3</identifier><identifier>PMID: 21877227</identifier><identifier>CODEN: ECOTEL</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Anthropogenic factors ; Biological diversity ; Bivalvia - drug effects ; Bivalvia - genetics ; DNA - isolation & purification ; Drift ; Earth and Environmental Science ; Ecology ; Ecosystem ; Ecosystems ; Ecotoxicology ; Environment ; Environmental Management ; Environmental Monitoring - methods ; Genetic aspects ; Genetic diversity ; Genetic drift ; Genetic Loci ; Genetic structure ; Genetic Variation ; Genetics ; Marine ecosystems ; Marine environment ; Marine environments ; Mollusks ; Oil pollution ; Oil spills ; Petroleum Pollution - adverse effects ; Population Density ; Population genetics ; Population number ; Populations ; Reduction ; Shellfish ; Spain ; Tankers (waterborne) ; Temporal logic ; Toxicants ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - toxicity</subject><ispartof>Ecotoxicology (London), 2012, Vol.21 (1), p.225-233</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><rights>COPYRIGHT 2012 Springer</rights><rights>Springer Science+Business Media, LLC 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-ffb5d5c3ec9861e4af402c2fd1ca5da13ac52f5e78d0bfac6f48e8e1246456143</citedby><cites>FETCH-LOGICAL-c441t-ffb5d5c3ec9861e4af402c2fd1ca5da13ac52f5e78d0bfac6f48e8e1246456143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10646-011-0781-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10646-011-0781-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21877227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernandez-Tajes, J</creatorcontrib><creatorcontrib>Arias-Perez, A</creatorcontrib><creatorcontrib>Fernandez-Moreno, M</creatorcontrib><creatorcontrib>Mendez, J</creatorcontrib><title>Sharp decrease of genetic variation in two Spanish localities of razor clam Ensis siliqua: natural fluctuation or Prestige oil spill effects?</title><title>Ecotoxicology (London)</title><addtitle>Ecotoxicology</addtitle><addtitle>Ecotoxicology</addtitle><description>Pollution is one of the main concerns in marine ecosystems nowadays. Environmental anthropogenic-mediated toxicants may affect genetic diversity both at the individual and ecosystem levels and may also alter the genetic structure of populations. This study examined the temporal pattern of genetic diversity among populations of the benthic bivalve
Ensis siliqua
in two locations of Galicia, following the Prestige oil spillage. On November 13, 2002 the oil tanker Prestige sank at 240 km from Galician coast and 63,000 tonnes of heavy fuel were released to the marine environment.
E. siliqua
samples were sampled between 2001 and 2006. Genetic variation was assessed by means of Random Amplification of Polymorphic DNA (RAPD). A significant decrease in genetic diversity was observed for the 2006 samples. Nei’s genetic distance, fixation index (PhiPT), and PCA values also supported differences in the 2006 samples. We hypothesize that the temporal genetic variation observed in
E. siliqua
populations is due to a strong effect of genetic drift caused by a reduction in population size and that the indirect effects of the Prestige spill possibly caused this reduction.</description><subject>Animals</subject><subject>Anthropogenic factors</subject><subject>Biological diversity</subject><subject>Bivalvia - drug effects</subject><subject>Bivalvia - genetics</subject><subject>DNA - isolation & purification</subject><subject>Drift</subject><subject>Earth and Environmental Science</subject><subject>Ecology</subject><subject>Ecosystem</subject><subject>Ecosystems</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Management</subject><subject>Environmental Monitoring - methods</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>Genetic drift</subject><subject>Genetic Loci</subject><subject>Genetic structure</subject><subject>Genetic Variation</subject><subject>Genetics</subject><subject>Marine ecosystems</subject><subject>Marine environment</subject><subject>Marine environments</subject><subject>Mollusks</subject><subject>Oil pollution</subject><subject>Oil spills</subject><subject>Petroleum Pollution - adverse effects</subject><subject>Population Density</subject><subject>Population genetics</subject><subject>Population number</subject><subject>Populations</subject><subject>Reduction</subject><subject>Shellfish</subject><subject>Spain</subject><subject>Tankers (waterborne)</subject><subject>Temporal logic</subject><subject>Toxicants</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0963-9292</issn><issn>1573-3017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kUFrFTEUhQdR7LP6A9xIcONqapLJTDJupJRWhYJCdR3uy9y8pmSS1yRT0f_gf24eUxUEyeJC8p2Twz1N85LRE0apfJsZHcTQUsZaKhVru0fNhvWyazvK5ONmQ8eha0c-8qPmWc43lNJRCvq0OeJMScm53DS_rq4h7cmEJiFkJNGSHQYszpA7SA6Ki4G4QMr3SK72EFy-Jj4a8K44zAc8wc-YiPEwk_OQXSbZeXe7wDsSoCwJPLF-MWVZrSr6JWEublf_cp7kvfOeoLVoSn7_vHliwWd88TCPm28X51_PPraXnz98Oju9bI0QrLTWbvupNx2aUQ0MBVhBueF2Ygb6CVgHpue2R6kmurVgBisUKmRcDKIfmOiOmzer7z7F26XG0bPLBr2HgHHJeuSDUooOfSVf_0PexCWFGk6P1YjWbasKnazQDjxqF2wsCUw9E87OxIDW1ftTyToplBzGKmCrwKSYc0Kr98nNkH5oRvWhWr1Wq2u1-lCt7qrm1UOSZTvj9Efxu8sK8BXI9SnsMP2N-n_Xe5UqsMc</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Fernandez-Tajes, J</creator><creator>Arias-Perez, A</creator><creator>Fernandez-Moreno, M</creator><creator>Mendez, J</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7SN</scope><scope>7ST</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>SOI</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>2012</creationdate><title>Sharp decrease of genetic variation in two Spanish localities of razor clam Ensis siliqua: natural fluctuation or Prestige oil spill effects?</title><author>Fernandez-Tajes, J ; Arias-Perez, A ; Fernandez-Moreno, M ; Mendez, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-ffb5d5c3ec9861e4af402c2fd1ca5da13ac52f5e78d0bfac6f48e8e1246456143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Anthropogenic factors</topic><topic>Biological diversity</topic><topic>Bivalvia - drug effects</topic><topic>Bivalvia - genetics</topic><topic>DNA - isolation & purification</topic><topic>Drift</topic><topic>Earth and Environmental Science</topic><topic>Ecology</topic><topic>Ecosystem</topic><topic>Ecosystems</topic><topic>Ecotoxicology</topic><topic>Environment</topic><topic>Environmental Management</topic><topic>Environmental Monitoring - methods</topic><topic>Genetic aspects</topic><topic>Genetic diversity</topic><topic>Genetic drift</topic><topic>Genetic Loci</topic><topic>Genetic structure</topic><topic>Genetic Variation</topic><topic>Genetics</topic><topic>Marine ecosystems</topic><topic>Marine environment</topic><topic>Marine environments</topic><topic>Mollusks</topic><topic>Oil pollution</topic><topic>Oil spills</topic><topic>Petroleum Pollution - adverse effects</topic><topic>Population Density</topic><topic>Population genetics</topic><topic>Population number</topic><topic>Populations</topic><topic>Reduction</topic><topic>Shellfish</topic><topic>Spain</topic><topic>Tankers (waterborne)</topic><topic>Temporal logic</topic><topic>Toxicants</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandez-Tajes, J</creatorcontrib><creatorcontrib>Arias-Perez, A</creatorcontrib><creatorcontrib>Fernandez-Moreno, M</creatorcontrib><creatorcontrib>Mendez, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Ecotoxicology (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandez-Tajes, J</au><au>Arias-Perez, A</au><au>Fernandez-Moreno, M</au><au>Mendez, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sharp decrease of genetic variation in two Spanish localities of razor clam Ensis siliqua: natural fluctuation or Prestige oil spill effects?</atitle><jtitle>Ecotoxicology (London)</jtitle><stitle>Ecotoxicology</stitle><addtitle>Ecotoxicology</addtitle><date>2012</date><risdate>2012</risdate><volume>21</volume><issue>1</issue><spage>225</spage><epage>233</epage><pages>225-233</pages><issn>0963-9292</issn><eissn>1573-3017</eissn><coden>ECOTEL</coden><abstract>Pollution is one of the main concerns in marine ecosystems nowadays. Environmental anthropogenic-mediated toxicants may affect genetic diversity both at the individual and ecosystem levels and may also alter the genetic structure of populations. This study examined the temporal pattern of genetic diversity among populations of the benthic bivalve
Ensis siliqua
in two locations of Galicia, following the Prestige oil spillage. On November 13, 2002 the oil tanker Prestige sank at 240 km from Galician coast and 63,000 tonnes of heavy fuel were released to the marine environment.
E. siliqua
samples were sampled between 2001 and 2006. Genetic variation was assessed by means of Random Amplification of Polymorphic DNA (RAPD). A significant decrease in genetic diversity was observed for the 2006 samples. Nei’s genetic distance, fixation index (PhiPT), and PCA values also supported differences in the 2006 samples. We hypothesize that the temporal genetic variation observed in
E. siliqua
populations is due to a strong effect of genetic drift caused by a reduction in population size and that the indirect effects of the Prestige spill possibly caused this reduction.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>21877227</pmid><doi>10.1007/s10646-011-0781-3</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0963-9292 |
| ispartof | Ecotoxicology (London), 2012, Vol.21 (1), p.225-233 |
| issn | 0963-9292 1573-3017 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_926888065 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Anthropogenic factors Biological diversity Bivalvia - drug effects Bivalvia - genetics DNA - isolation & purification Drift Earth and Environmental Science Ecology Ecosystem Ecosystems Ecotoxicology Environment Environmental Management Environmental Monitoring - methods Genetic aspects Genetic diversity Genetic drift Genetic Loci Genetic structure Genetic Variation Genetics Marine ecosystems Marine environment Marine environments Mollusks Oil pollution Oil spills Petroleum Pollution - adverse effects Population Density Population genetics Population number Populations Reduction Shellfish Spain Tankers (waterborne) Temporal logic Toxicants Water Pollutants, Chemical - analysis Water Pollutants, Chemical - toxicity |
| title | Sharp decrease of genetic variation in two Spanish localities of razor clam Ensis siliqua: natural fluctuation or Prestige oil spill effects? |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T05%3A36%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sharp%20decrease%20of%20genetic%20variation%20in%20two%20Spanish%20localities%20of%20razor%20clam%20Ensis%20siliqua:%20natural%20fluctuation%20or%20Prestige%20oil%20spill%20effects?&rft.jtitle=Ecotoxicology%20(London)&rft.au=Fernandez-Tajes,%20J&rft.date=2012&rft.volume=21&rft.issue=1&rft.spage=225&rft.epage=233&rft.pages=225-233&rft.issn=0963-9292&rft.eissn=1573-3017&rft.coden=ECOTEL&rft_id=info:doi/10.1007/s10646-011-0781-3&rft_dat=%3Cgale_proqu%3EA713748769%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=914305738&rft_id=info:pmid/21877227&rft_galeid=A713748769&rfr_iscdi=true |