Cholesterol assimilation and biotransformation by Lactobacillus helveticus
Lactobacillus helveticus , grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg −1 of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.04 g of andros...
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Veröffentlicht in: | Biotechnology letters 2012, Vol.34 (1), p.103-107 |
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container_title | Biotechnology letters |
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creator | Ahire, Jayesh J. Bhat, Anuja A. Thakare, Jayashri M. Pawar, Pallavi B. Zope, Dipali G. Jain, Rakeshkumar M. Chaudhari, Bhushan L. |
description | Lactobacillus helveticus
, grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg
−1
of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.04 g of androst-4-ene-3, 17 dione within 48 h at 37°C with extracellular cholesterol oxidase activity at 12 U mg
−1
and intracellular oxidase at 0.5 U mg
−1
. |
doi_str_mv | 10.1007/s10529-011-0733-2 |
format | Article |
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, grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg
−1
of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.04 g of androst-4-ene-3, 17 dione within 48 h at 37°C with extracellular cholesterol oxidase activity at 12 U mg
−1
and intracellular oxidase at 0.5 U mg
−1
.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-011-0733-2</identifier><identifier>PMID: 21898131</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Androstadienes - metabolism ; Applied Microbiology ; Biochemistry ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Biotransformation ; Cholesterol ; Cholesterol - metabolism ; Cholesterol oxidase ; Cholesterol Oxidase - metabolism ; Culture Media - chemistry ; Dione ; Fundamental and applied biological sciences. Psychology ; Lactobacillus helveticus ; Lactobacillus helveticus - metabolism ; Life Sciences ; Magnesium ; Methods. Procedures. Technologies ; Microbiology ; Original Research Paper ; Oxidase ; Oxidation ; Temperature ; Time Factors</subject><ispartof>Biotechnology letters, 2012, Vol.34 (1), p.103-107</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-b92ec229a12ab50776ce3207083d5b50e15050cb3b9f5af757243acb44632f1d3</citedby><cites>FETCH-LOGICAL-c465t-b92ec229a12ab50776ce3207083d5b50e15050cb3b9f5af757243acb44632f1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-011-0733-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-011-0733-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,4021,27921,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25577282$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21898131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahire, Jayesh J.</creatorcontrib><creatorcontrib>Bhat, Anuja A.</creatorcontrib><creatorcontrib>Thakare, Jayashri M.</creatorcontrib><creatorcontrib>Pawar, Pallavi B.</creatorcontrib><creatorcontrib>Zope, Dipali G.</creatorcontrib><creatorcontrib>Jain, Rakeshkumar M.</creatorcontrib><creatorcontrib>Chaudhari, Bhushan L.</creatorcontrib><title>Cholesterol assimilation and biotransformation by Lactobacillus helveticus</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Lactobacillus helveticus
, grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg
−1
of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.04 g of androst-4-ene-3, 17 dione within 48 h at 37°C with extracellular cholesterol oxidase activity at 12 U mg
−1
and intracellular oxidase at 0.5 U mg
−1
.</description><subject>Androstadienes - metabolism</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Biotransformation</subject><subject>Cholesterol</subject><subject>Cholesterol - metabolism</subject><subject>Cholesterol oxidase</subject><subject>Cholesterol Oxidase - metabolism</subject><subject>Culture Media - chemistry</subject><subject>Dione</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lactobacillus helveticus</subject><subject>Lactobacillus helveticus - metabolism</subject><subject>Life Sciences</subject><subject>Magnesium</subject><subject>Methods. Procedures. 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Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Biotransformation</topic><topic>Cholesterol</topic><topic>Cholesterol - metabolism</topic><topic>Cholesterol oxidase</topic><topic>Cholesterol Oxidase - metabolism</topic><topic>Culture Media - chemistry</topic><topic>Dione</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lactobacillus helveticus</topic><topic>Lactobacillus helveticus - metabolism</topic><topic>Life Sciences</topic><topic>Magnesium</topic><topic>Methods. Procedures. 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, grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg
−1
of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.04 g of androst-4-ene-3, 17 dione within 48 h at 37°C with extracellular cholesterol oxidase activity at 12 U mg
−1
and intracellular oxidase at 0.5 U mg
−1
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21898131</pmid><doi>10.1007/s10529-011-0733-2</doi><tpages>5</tpages></addata></record> |
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subjects | Androstadienes - metabolism Applied Microbiology Biochemistry Bioconversions. Hemisynthesis Biological and medical sciences Biomedical and Life Sciences Biotechnology Biotransformation Cholesterol Cholesterol - metabolism Cholesterol oxidase Cholesterol Oxidase - metabolism Culture Media - chemistry Dione Fundamental and applied biological sciences. Psychology Lactobacillus helveticus Lactobacillus helveticus - metabolism Life Sciences Magnesium Methods. Procedures. Technologies Microbiology Original Research Paper Oxidase Oxidation Temperature Time Factors |
title | Cholesterol assimilation and biotransformation by Lactobacillus helveticus |
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