Differential effector responses by circulating/blood and tissue/peritoneal neutrophils following burn combined with Enterococcus faecalis infection
Abstract Recently we found that superimposition of Enterococcus faecalis infection on burn injury caused an eruption of host mortality not seen with either individual challenge. We hypothesized that the Enterococcus bacteria, and/or factors related to these organisms, aggravate burn-induced modulati...
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| creator | Fazal, Nadeem Shelip, Alla Siddiqui, Erum Ali, Ashraf Azim, Anser C. Al-Ghoul, Walid M. |
| description | Abstract
Recently we found that superimposition of Enterococcus faecalis infection on burn injury caused an eruption of host mortality not seen with either individual challenge. We hypothesized that the Enterococcus bacteria, and/or factors related to these organisms, aggravate burn-induced modulations in host defense by neutrophils. Our study focuses on alterations in neutrophils' oxidative, proteolytic, and adhesive functions and transendothelial migration of neutrophils in burn rats inoculated with E. faecalis. Rats were subjected to burn (30% total body surface area) and then intra-abdominally inoculated with E. faecalis (104CFU kg−1 b.w). Polymorphonuclear neutrophils (PMNs) were harvested from circulating/blood and tissue/peritoneal cavity at day-2 post injury. Extracellular release of O−2 anion production was determined by luminometry, and intracellular production of reactive oxygen species was measured by digital imaging technique. Fluoroscan analysis and confocal microscopy determined intracellular elastase production. The expression of adhesion molecule CD11b/CD18 was performed by flow cytometry. Calcein AM-labeled PMNs were co-cultured with TNF-α-stimulated rat lung microvascular endothelial cells, and their ability to adhere was assessed by fluorometry and digital imaging and finally, chemotaxis was measured by neutrophil transmigration assays. The results showed differential effector responses by circulatory and/or tissue PMNs. Tissue/peritoneal PMNs produced more O−2 less intracellular elastase, and increased expression of CD11b/CD18 accompanied with increased adhesivity of MIP-2-stimulated PMNs to endothelial cells as compared to circulatory/blood PMNs. This differential effect was more pronounced following burn plus E. faecalis infection, indicating that the combined injury changed neutrophil functions. |
| doi_str_mv | 10.1111/j.1574-695X.2011.00881.x |
| format | Article |
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Recently we found that superimposition of Enterococcus faecalis infection on burn injury caused an eruption of host mortality not seen with either individual challenge. We hypothesized that the Enterococcus bacteria, and/or factors related to these organisms, aggravate burn-induced modulations in host defense by neutrophils. Our study focuses on alterations in neutrophils' oxidative, proteolytic, and adhesive functions and transendothelial migration of neutrophils in burn rats inoculated with E. faecalis. Rats were subjected to burn (30% total body surface area) and then intra-abdominally inoculated with E. faecalis (104CFU kg−1 b.w). Polymorphonuclear neutrophils (PMNs) were harvested from circulating/blood and tissue/peritoneal cavity at day-2 post injury. Extracellular release of O−2 anion production was determined by luminometry, and intracellular production of reactive oxygen species was measured by digital imaging technique. Fluoroscan analysis and confocal microscopy determined intracellular elastase production. The expression of adhesion molecule CD11b/CD18 was performed by flow cytometry. Calcein AM-labeled PMNs were co-cultured with TNF-α-stimulated rat lung microvascular endothelial cells, and their ability to adhere was assessed by fluorometry and digital imaging and finally, chemotaxis was measured by neutrophil transmigration assays. The results showed differential effector responses by circulatory and/or tissue PMNs. Tissue/peritoneal PMNs produced more O−2 less intracellular elastase, and increased expression of CD11b/CD18 accompanied with increased adhesivity of MIP-2-stimulated PMNs to endothelial cells as compared to circulatory/blood PMNs. This differential effect was more pronounced following burn plus E. faecalis infection, indicating that the combined injury changed neutrophil functions.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1111/j.1574-695X.2011.00881.x</identifier><identifier>PMID: 22066701</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adhesion ; Animals ; Bacteriology ; Biological and medical sciences ; Blood ; Blood circulation ; burn ; Burns - blood ; Burns - immunology ; Burns - metabolism ; Burns - microbiology ; Calcein ; CD11b antigen ; CD11b Antigen - metabolism ; CD18 antigen ; CD18 Antigens - metabolism ; Cell Adhesion - immunology ; Chemotaxis ; Confocal microscopy ; Digital imaging ; E. faecalis ; Elastase ; Endothelial cells ; Enterococcus ; Enterococcus faecalis ; Enterococcus faecalis - immunology ; Flow cytometry ; Fluorimetry ; Fluorometry ; Fundamental and applied biological sciences. Psychology ; Gram-Positive Bacterial Infections - blood ; Gram-Positive Bacterial Infections - immunology ; Gram-Positive Bacterial Infections - metabolism ; Gram-Positive Bacterial Infections - microbiology ; infection ; Infections ; Injuries ; Intracellular ; Leukocyte migration ; Leukocytes (neutrophilic) ; Leukocytes (polymorphonuclear) ; Male ; Microbiology ; Microscopy, Fluorescence ; Microvasculature ; Miscellaneous ; Neutrophils ; Neutrophils - immunology ; Neutrophils - metabolism ; Oxygen - metabolism ; oxygen burst ; Pancreatic Elastase - metabolism ; Peritoneal Cavity - cytology ; Peritoneum ; Proteolysis ; Rats ; Rats, Sprague-Dawley ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Tumor necrosis factor-α</subject><ispartof>FEMS immunology and medical microbiology, 2012-03, Vol.64 (2), p.191-204</ispartof><rights>2011 Federation of European Microbiological Societies. 2011</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>2011 Federation of European Microbiological Societies.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5521-dad1de4cb0dff8505afeaa7f8cd7e1c7a5d8d20c0fdd83f9804a0fc6e1b6c11d3</citedby><cites>FETCH-LOGICAL-c5521-dad1de4cb0dff8505afeaa7f8cd7e1c7a5d8d20c0fdd83f9804a0fc6e1b6c11d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-695X.2011.00881.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-695X.2011.00881.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25502522$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22066701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fazal, Nadeem</creatorcontrib><creatorcontrib>Shelip, Alla</creatorcontrib><creatorcontrib>Siddiqui, Erum</creatorcontrib><creatorcontrib>Ali, Ashraf</creatorcontrib><creatorcontrib>Azim, Anser C.</creatorcontrib><creatorcontrib>Al-Ghoul, Walid M.</creatorcontrib><title>Differential effector responses by circulating/blood and tissue/peritoneal neutrophils following burn combined with Enterococcus faecalis infection</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>Abstract
Recently we found that superimposition of Enterococcus faecalis infection on burn injury caused an eruption of host mortality not seen with either individual challenge. We hypothesized that the Enterococcus bacteria, and/or factors related to these organisms, aggravate burn-induced modulations in host defense by neutrophils. Our study focuses on alterations in neutrophils' oxidative, proteolytic, and adhesive functions and transendothelial migration of neutrophils in burn rats inoculated with E. faecalis. Rats were subjected to burn (30% total body surface area) and then intra-abdominally inoculated with E. faecalis (104CFU kg−1 b.w). Polymorphonuclear neutrophils (PMNs) were harvested from circulating/blood and tissue/peritoneal cavity at day-2 post injury. Extracellular release of O−2 anion production was determined by luminometry, and intracellular production of reactive oxygen species was measured by digital imaging technique. Fluoroscan analysis and confocal microscopy determined intracellular elastase production. The expression of adhesion molecule CD11b/CD18 was performed by flow cytometry. Calcein AM-labeled PMNs were co-cultured with TNF-α-stimulated rat lung microvascular endothelial cells, and their ability to adhere was assessed by fluorometry and digital imaging and finally, chemotaxis was measured by neutrophil transmigration assays. The results showed differential effector responses by circulatory and/or tissue PMNs. Tissue/peritoneal PMNs produced more O−2 less intracellular elastase, and increased expression of CD11b/CD18 accompanied with increased adhesivity of MIP-2-stimulated PMNs to endothelial cells as compared to circulatory/blood PMNs. This differential effect was more pronounced following burn plus E. faecalis infection, indicating that the combined injury changed neutrophil functions.</description><subject>Adhesion</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Blood circulation</subject><subject>burn</subject><subject>Burns - blood</subject><subject>Burns - immunology</subject><subject>Burns - metabolism</subject><subject>Burns - microbiology</subject><subject>Calcein</subject><subject>CD11b antigen</subject><subject>CD11b Antigen - metabolism</subject><subject>CD18 antigen</subject><subject>CD18 Antigens - metabolism</subject><subject>Cell Adhesion - immunology</subject><subject>Chemotaxis</subject><subject>Confocal microscopy</subject><subject>Digital imaging</subject><subject>E. faecalis</subject><subject>Elastase</subject><subject>Endothelial cells</subject><subject>Enterococcus</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - immunology</subject><subject>Flow cytometry</subject><subject>Fluorimetry</subject><subject>Fluorometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gram-Positive Bacterial Infections - blood</subject><subject>Gram-Positive Bacterial Infections - immunology</subject><subject>Gram-Positive Bacterial Infections - metabolism</subject><subject>Gram-Positive Bacterial Infections - microbiology</subject><subject>infection</subject><subject>Infections</subject><subject>Injuries</subject><subject>Intracellular</subject><subject>Leukocyte migration</subject><subject>Leukocytes (neutrophilic)</subject><subject>Leukocytes (polymorphonuclear)</subject><subject>Male</subject><subject>Microbiology</subject><subject>Microscopy, Fluorescence</subject><subject>Microvasculature</subject><subject>Miscellaneous</subject><subject>Neutrophils</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - metabolism</subject><subject>Oxygen - metabolism</subject><subject>oxygen burst</subject><subject>Pancreatic Elastase - metabolism</subject><subject>Peritoneal Cavity - cytology</subject><subject>Peritoneum</subject><subject>Proteolysis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Tumor necrosis factor-α</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdGK1DAUhoso7rj6ChIQcW_aSdKmTcEbWXd1YcUbBe9Cmpy4GTJJTVpm5zl84U2dcQVFMTcJ5PtzTs5XFIjgiuS13lSEdU3Z9uxLRTEhFcack-r2QbG6v3hYrHBPeclp05wUT1LaYIybHuPHxQmluG07TFbF97fWGIjgJysdgnxWU4goQhqDT5DQsEfKRjU7OVn_dT24EDSSXqPJpjTDeoRop-Ahpz3MUwzjjXUJmeBc2OUEGubokQrbwXrQaGenG3ThJ4hBBaXmTEpQ0tmErF-K2-CfFo-MdAmeHffT4vPlxafz9-X1x3dX52-uS8UYJaWWmmho1IC1MZxhJg1I2RmudAdEdZJprilW2GjNa9Nz3EhsVAtkaBUhuj4tXh3eHWP4NkOaxNYmBc5JD2FOoqct54z1JJNn_yRJdkC6tmtYRl_8hm5CnkD-h6A1bmndNnWTKX6gVAwpRTBijHYr4z4_JRbFYiMWk2IxKRbF4odicZujz48F5mEL-j7402kGXh4BmfJkTZRe2fSLYwxTRmnmXh-4nXWw_-8GxOXVh3zI8foQD_P4l3D5Z_d3tFDWDA</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>Fazal, Nadeem</creator><creator>Shelip, Alla</creator><creator>Siddiqui, Erum</creator><creator>Ali, Ashraf</creator><creator>Azim, Anser C.</creator><creator>Al-Ghoul, Walid M.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>7QL</scope><scope>7QR</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201203</creationdate><title>Differential effector responses by circulating/blood and tissue/peritoneal neutrophils following burn combined with Enterococcus faecalis infection</title><author>Fazal, Nadeem ; Shelip, Alla ; Siddiqui, Erum ; Ali, Ashraf ; Azim, Anser C. ; Al-Ghoul, Walid M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5521-dad1de4cb0dff8505afeaa7f8cd7e1c7a5d8d20c0fdd83f9804a0fc6e1b6c11d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adhesion</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Blood circulation</topic><topic>burn</topic><topic>Burns - blood</topic><topic>Burns - immunology</topic><topic>Burns - metabolism</topic><topic>Burns - microbiology</topic><topic>Calcein</topic><topic>CD11b antigen</topic><topic>CD11b Antigen - metabolism</topic><topic>CD18 antigen</topic><topic>CD18 Antigens - metabolism</topic><topic>Cell Adhesion - immunology</topic><topic>Chemotaxis</topic><topic>Confocal microscopy</topic><topic>Digital imaging</topic><topic>E. faecalis</topic><topic>Elastase</topic><topic>Endothelial cells</topic><topic>Enterococcus</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - immunology</topic><topic>Flow cytometry</topic><topic>Fluorimetry</topic><topic>Fluorometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gram-Positive Bacterial Infections - blood</topic><topic>Gram-Positive Bacterial Infections - immunology</topic><topic>Gram-Positive Bacterial Infections - metabolism</topic><topic>Gram-Positive Bacterial Infections - microbiology</topic><topic>infection</topic><topic>Infections</topic><topic>Injuries</topic><topic>Intracellular</topic><topic>Leukocyte migration</topic><topic>Leukocytes (neutrophilic)</topic><topic>Leukocytes (polymorphonuclear)</topic><topic>Male</topic><topic>Microbiology</topic><topic>Microscopy, Fluorescence</topic><topic>Microvasculature</topic><topic>Miscellaneous</topic><topic>Neutrophils</topic><topic>Neutrophils - immunology</topic><topic>Neutrophils - metabolism</topic><topic>Oxygen - metabolism</topic><topic>oxygen burst</topic><topic>Pancreatic Elastase - metabolism</topic><topic>Peritoneal Cavity - cytology</topic><topic>Peritoneum</topic><topic>Proteolysis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fazal, Nadeem</creatorcontrib><creatorcontrib>Shelip, Alla</creatorcontrib><creatorcontrib>Siddiqui, Erum</creatorcontrib><creatorcontrib>Ali, Ashraf</creatorcontrib><creatorcontrib>Azim, Anser C.</creatorcontrib><creatorcontrib>Al-Ghoul, Walid M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fazal, Nadeem</au><au>Shelip, Alla</au><au>Siddiqui, Erum</au><au>Ali, Ashraf</au><au>Azim, Anser C.</au><au>Al-Ghoul, Walid M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential effector responses by circulating/blood and tissue/peritoneal neutrophils following burn combined with Enterococcus faecalis infection</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2012-03</date><risdate>2012</risdate><volume>64</volume><issue>2</issue><spage>191</spage><epage>204</epage><pages>191-204</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>Abstract
Recently we found that superimposition of Enterococcus faecalis infection on burn injury caused an eruption of host mortality not seen with either individual challenge. We hypothesized that the Enterococcus bacteria, and/or factors related to these organisms, aggravate burn-induced modulations in host defense by neutrophils. Our study focuses on alterations in neutrophils' oxidative, proteolytic, and adhesive functions and transendothelial migration of neutrophils in burn rats inoculated with E. faecalis. Rats were subjected to burn (30% total body surface area) and then intra-abdominally inoculated with E. faecalis (104CFU kg−1 b.w). Polymorphonuclear neutrophils (PMNs) were harvested from circulating/blood and tissue/peritoneal cavity at day-2 post injury. Extracellular release of O−2 anion production was determined by luminometry, and intracellular production of reactive oxygen species was measured by digital imaging technique. Fluoroscan analysis and confocal microscopy determined intracellular elastase production. The expression of adhesion molecule CD11b/CD18 was performed by flow cytometry. Calcein AM-labeled PMNs were co-cultured with TNF-α-stimulated rat lung microvascular endothelial cells, and their ability to adhere was assessed by fluorometry and digital imaging and finally, chemotaxis was measured by neutrophil transmigration assays. The results showed differential effector responses by circulatory and/or tissue PMNs. Tissue/peritoneal PMNs produced more O−2 less intracellular elastase, and increased expression of CD11b/CD18 accompanied with increased adhesivity of MIP-2-stimulated PMNs to endothelial cells as compared to circulatory/blood PMNs. This differential effect was more pronounced following burn plus E. faecalis infection, indicating that the combined injury changed neutrophil functions.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22066701</pmid><doi>10.1111/j.1574-695X.2011.00881.x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEMS immunology and medical microbiology, 2012-03, Vol.64 (2), p.191-204 |
| issn | 0928-8244 1574-695X 2049-632X |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adhesion Animals Bacteriology Biological and medical sciences Blood Blood circulation burn Burns - blood Burns - immunology Burns - metabolism Burns - microbiology Calcein CD11b antigen CD11b Antigen - metabolism CD18 antigen CD18 Antigens - metabolism Cell Adhesion - immunology Chemotaxis Confocal microscopy Digital imaging E. faecalis Elastase Endothelial cells Enterococcus Enterococcus faecalis Enterococcus faecalis - immunology Flow cytometry Fluorimetry Fluorometry Fundamental and applied biological sciences. Psychology Gram-Positive Bacterial Infections - blood Gram-Positive Bacterial Infections - immunology Gram-Positive Bacterial Infections - metabolism Gram-Positive Bacterial Infections - microbiology infection Infections Injuries Intracellular Leukocyte migration Leukocytes (neutrophilic) Leukocytes (polymorphonuclear) Male Microbiology Microscopy, Fluorescence Microvasculature Miscellaneous Neutrophils Neutrophils - immunology Neutrophils - metabolism Oxygen - metabolism oxygen burst Pancreatic Elastase - metabolism Peritoneal Cavity - cytology Peritoneum Proteolysis Rats Rats, Sprague-Dawley Reactive oxygen species Reactive Oxygen Species - metabolism Tumor necrosis factor-α |
| title | Differential effector responses by circulating/blood and tissue/peritoneal neutrophils following burn combined with Enterococcus faecalis infection |
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