Global transcriptome analysis of the tetrachloroethene-dechlorinating bacterium Desulfitobacterium hafniense Y51 in the presence of various electron donors and terminal electron acceptors
Desulfitobacterium hafniense Y51 is a dechlorinating bacterium that encodes an unusually large set of O-demethylase paralogs and specialized respiratory systems including specialized electron donors and acceptors. To use this organism in bioremediation of tetrachloroethene (PCE) or trichloroethene (...
Gespeichert in:
| Veröffentlicht in: | Journal of industrial microbiology & biotechnology 2012-02, Vol.39 (2), p.255-268 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 268 |
|---|---|
| container_issue | 2 |
| container_start_page | 255 |
| container_title | Journal of industrial microbiology & biotechnology |
| container_volume | 39 |
| creator | Peng, Xue Yamamoto, Shogo Vertès, Alain A Keresztes, Gabor Inatomi, Ken-ichi Inui, Masayuki Yukawa, Hideaki |
| description | Desulfitobacterium hafniense Y51 is a dechlorinating bacterium that encodes an unusually large set of O-demethylase paralogs and specialized respiratory systems including specialized electron donors and acceptors. To use this organism in bioremediation of tetrachloroethene (PCE) or trichloroethene (TCE) pollution, expression patterns of its 5,060 genes were determined under different conditions using 60-mer probes in DNA microarrays. PCE, TCE, fumarate, nitrate, and dimethyl sulfoxide (DMSO) respiration all sustain the growth of strain Y51. Global transcriptome analyses were thus performed using various electron donor and acceptor couples (respectively, pyruvate and either fumarate, TCE, nitrate, or DMSO, and vanillate/fumarate). When TCE is used as terminal electron acceptor, resulting in its detoxification, a series of electron carriers comprising a cytochrome bd-type quinol oxidase (DSY4055-4056), a ferredoxin (DSY1451), and four Fe–S proteins (DSY1626, DSY1629, DSY0733, DSY3309) are upregulated, suggesting that the products of these genes are involved in PCE oxidoreduction. Interestingly, the PCE dehalogenase cluster (pceABCT) is constitutively expressed in the media tested, with pceT being upregulated and pceC downregulated in pyruvate/TCE-containing medium. In addition, another dehalogenation enzyme (DSY1155 coding for a putative chlorophenol reductive dehalogenase), is induced 225-fold in that medium, despite not being involved in PCE respiration. Remarkably since the reducing equivalents formed during pyruvate conversion to acetyl-CoA are channeled to electron acceptors including halogenated compounds, pyruvate induces expression of a pyruvate:ferredoxin oxidoreductase. This study paves the way to understanding the physiology of D. hafniense, optimizing this microbe as a bioremediation agent, and designing bioarray sensors to monitor the presence of dechlorinating organisms in the environment. |
| doi_str_mv | 10.1007/s10295-011-1023-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_926885468</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>926885468</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-f7b3b3c3bc40412359061d65eda0aca87420114a9b0df13d41e427f5105a14b3</originalsourceid><addsrcrecordid>eNp9ks9u1DAQxiMEon_gAbiAhVRxCtixHSfHqkBBqsSBcuAUTZzJrivHXuwEqc_GyzHbLKzEgZPHnt_MfJrPRfFC8LeCc_MuC161uuRClBTJ0jwqToUydam11I8plrUptZL6pDjL-Y5zro2pnhYnlWhqIXRzWvy69rEHz-YEIdvkdnOckEEAf59dZnFk8xbZjJS3Wx9TRLoHLAd8uLoAswsb1oOdMbllYu8xL350czw-bWEMDkNG9l0L5sJDy13CjMHifsRPSC4umaFHO6cY2BBDTJlkDDQ6TTTFH5NgLZLMlJ8VT0bwGZ8fzvPi9uOH26tP5c2X689XlzelVW07l6PpZS-t7K3iSlRSt7wWQ61xAA4WGqMq2qCCtufDKOSgBKrKjFpwDUL18rx4s7bdpfhjwTx3k8sWvYeApLprq7pptKobIl__Q97FJZF4goSRVV2pmiCxQjbFnBOO3S65CdJ9J3i3t7Vbbe1IVLe3tTNU8_LQeOknHP5W_PGRgIsDANmCH8lN6_KR07WQmrfEVSuXKRU2mI4K_zf91Vo0Quxgk6jxt6-0M0UfSrWNbuVvuZ3IBA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>917326246</pqid></control><display><type>article</type><title>Global transcriptome analysis of the tetrachloroethene-dechlorinating bacterium Desulfitobacterium hafniense Y51 in the presence of various electron donors and terminal electron acceptors</title><source>Oxford Journals Open Access Collection</source><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Peng, Xue ; Yamamoto, Shogo ; Vertès, Alain A ; Keresztes, Gabor ; Inatomi, Ken-ichi ; Inui, Masayuki ; Yukawa, Hideaki</creator><creatorcontrib>Peng, Xue ; Yamamoto, Shogo ; Vertès, Alain A ; Keresztes, Gabor ; Inatomi, Ken-ichi ; Inui, Masayuki ; Yukawa, Hideaki</creatorcontrib><description>Desulfitobacterium hafniense Y51 is a dechlorinating bacterium that encodes an unusually large set of O-demethylase paralogs and specialized respiratory systems including specialized electron donors and acceptors. To use this organism in bioremediation of tetrachloroethene (PCE) or trichloroethene (TCE) pollution, expression patterns of its 5,060 genes were determined under different conditions using 60-mer probes in DNA microarrays. PCE, TCE, fumarate, nitrate, and dimethyl sulfoxide (DMSO) respiration all sustain the growth of strain Y51. Global transcriptome analyses were thus performed using various electron donor and acceptor couples (respectively, pyruvate and either fumarate, TCE, nitrate, or DMSO, and vanillate/fumarate). When TCE is used as terminal electron acceptor, resulting in its detoxification, a series of electron carriers comprising a cytochrome bd-type quinol oxidase (DSY4055-4056), a ferredoxin (DSY1451), and four Fe–S proteins (DSY1626, DSY1629, DSY0733, DSY3309) are upregulated, suggesting that the products of these genes are involved in PCE oxidoreduction. Interestingly, the PCE dehalogenase cluster (pceABCT) is constitutively expressed in the media tested, with pceT being upregulated and pceC downregulated in pyruvate/TCE-containing medium. In addition, another dehalogenation enzyme (DSY1155 coding for a putative chlorophenol reductive dehalogenase), is induced 225-fold in that medium, despite not being involved in PCE respiration. Remarkably since the reducing equivalents formed during pyruvate conversion to acetyl-CoA are channeled to electron acceptors including halogenated compounds, pyruvate induces expression of a pyruvate:ferredoxin oxidoreductase. This study paves the way to understanding the physiology of D. hafniense, optimizing this microbe as a bioremediation agent, and designing bioarray sensors to monitor the presence of dechlorinating organisms in the environment.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1007/s10295-011-1023-7</identifier><identifier>PMID: 21861158</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>acetyl coenzyme A ; Analysis ; Animals ; Bacteria ; Biochemistry ; Biodegradation, Environmental ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Bioremediation ; Biotechnology ; Chemical engineering ; Chlorophenol ; dehalogenation ; Desulfitobacterium - genetics ; Desulfitobacterium - growth & development ; Desulfitobacterium - metabolism ; Desulfitobacterium hafniense ; Detoxification ; dimethyl sulfoxide ; DNA probes ; Electrons ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Profiling ; gene expression regulation ; Genes ; Genetic Engineering ; Genomes ; Halogenated compounds ; Halogenation ; Hydrogen ; Inorganic Chemistry ; Iron-Sulfur Proteins - genetics ; Iron-Sulfur Proteins - metabolism ; Laboratories ; Life Sciences ; microarray technology ; Microbiology ; Nitrates ; Original Paper ; Oxidants - metabolism ; Oxidation-Reduction ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Oxidoreductases, O-Demethylating - genetics ; Oxidoreductases, O-Demethylating - metabolism ; Physiology ; pollution ; Proteins ; pyruvate synthase ; pyruvic acid ; Respiration ; Respiratory system ; Soil contamination ; Studies ; Succinate Dehydrogenase - genetics ; Succinate Dehydrogenase - metabolism ; Tetrachloroethylene ; Tetrachloroethylene - metabolism ; Transcriptome ; transcriptomics ; Trichloroethylene - metabolism ; Water Pollutants, Chemical - metabolism</subject><ispartof>Journal of industrial microbiology & biotechnology, 2012-02, Vol.39 (2), p.255-268</ispartof><rights>Society for Industrial Microbiology 2011</rights><rights>2015 INIST-CNRS</rights><rights>Society for Industrial Microbiology and Biotechnology 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-f7b3b3c3bc40412359061d65eda0aca87420114a9b0df13d41e427f5105a14b3</citedby><cites>FETCH-LOGICAL-c499t-f7b3b3c3bc40412359061d65eda0aca87420114a9b0df13d41e427f5105a14b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10295-011-1023-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10295-011-1023-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25613509$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21861158$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, Xue</creatorcontrib><creatorcontrib>Yamamoto, Shogo</creatorcontrib><creatorcontrib>Vertès, Alain A</creatorcontrib><creatorcontrib>Keresztes, Gabor</creatorcontrib><creatorcontrib>Inatomi, Ken-ichi</creatorcontrib><creatorcontrib>Inui, Masayuki</creatorcontrib><creatorcontrib>Yukawa, Hideaki</creatorcontrib><title>Global transcriptome analysis of the tetrachloroethene-dechlorinating bacterium Desulfitobacterium hafniense Y51 in the presence of various electron donors and terminal electron acceptors</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><description>Desulfitobacterium hafniense Y51 is a dechlorinating bacterium that encodes an unusually large set of O-demethylase paralogs and specialized respiratory systems including specialized electron donors and acceptors. To use this organism in bioremediation of tetrachloroethene (PCE) or trichloroethene (TCE) pollution, expression patterns of its 5,060 genes were determined under different conditions using 60-mer probes in DNA microarrays. PCE, TCE, fumarate, nitrate, and dimethyl sulfoxide (DMSO) respiration all sustain the growth of strain Y51. Global transcriptome analyses were thus performed using various electron donor and acceptor couples (respectively, pyruvate and either fumarate, TCE, nitrate, or DMSO, and vanillate/fumarate). When TCE is used as terminal electron acceptor, resulting in its detoxification, a series of electron carriers comprising a cytochrome bd-type quinol oxidase (DSY4055-4056), a ferredoxin (DSY1451), and four Fe–S proteins (DSY1626, DSY1629, DSY0733, DSY3309) are upregulated, suggesting that the products of these genes are involved in PCE oxidoreduction. Interestingly, the PCE dehalogenase cluster (pceABCT) is constitutively expressed in the media tested, with pceT being upregulated and pceC downregulated in pyruvate/TCE-containing medium. In addition, another dehalogenation enzyme (DSY1155 coding for a putative chlorophenol reductive dehalogenase), is induced 225-fold in that medium, despite not being involved in PCE respiration. Remarkably since the reducing equivalents formed during pyruvate conversion to acetyl-CoA are channeled to electron acceptors including halogenated compounds, pyruvate induces expression of a pyruvate:ferredoxin oxidoreductase. This study paves the way to understanding the physiology of D. hafniense, optimizing this microbe as a bioremediation agent, and designing bioarray sensors to monitor the presence of dechlorinating organisms in the environment.</description><subject>acetyl coenzyme A</subject><subject>Analysis</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biodegradation, Environmental</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Bioremediation</subject><subject>Biotechnology</subject><subject>Chemical engineering</subject><subject>Chlorophenol</subject><subject>dehalogenation</subject><subject>Desulfitobacterium - genetics</subject><subject>Desulfitobacterium - growth & development</subject><subject>Desulfitobacterium - metabolism</subject><subject>Desulfitobacterium hafniense</subject><subject>Detoxification</subject><subject>dimethyl sulfoxide</subject><subject>DNA probes</subject><subject>Electrons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Profiling</subject><subject>gene expression regulation</subject><subject>Genes</subject><subject>Genetic Engineering</subject><subject>Genomes</subject><subject>Halogenated compounds</subject><subject>Halogenation</subject><subject>Hydrogen</subject><subject>Inorganic Chemistry</subject><subject>Iron-Sulfur Proteins - genetics</subject><subject>Iron-Sulfur Proteins - metabolism</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>microarray technology</subject><subject>Microbiology</subject><subject>Nitrates</subject><subject>Original Paper</subject><subject>Oxidants - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Oxidoreductases, O-Demethylating - genetics</subject><subject>Oxidoreductases, O-Demethylating - metabolism</subject><subject>Physiology</subject><subject>pollution</subject><subject>Proteins</subject><subject>pyruvate synthase</subject><subject>pyruvic acid</subject><subject>Respiration</subject><subject>Respiratory system</subject><subject>Soil contamination</subject><subject>Studies</subject><subject>Succinate Dehydrogenase - genetics</subject><subject>Succinate Dehydrogenase - metabolism</subject><subject>Tetrachloroethylene</subject><subject>Tetrachloroethylene - metabolism</subject><subject>Transcriptome</subject><subject>transcriptomics</subject><subject>Trichloroethylene - metabolism</subject><subject>Water Pollutants, Chemical - metabolism</subject><issn>1367-5435</issn><issn>1476-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9ks9u1DAQxiMEon_gAbiAhVRxCtixHSfHqkBBqsSBcuAUTZzJrivHXuwEqc_GyzHbLKzEgZPHnt_MfJrPRfFC8LeCc_MuC161uuRClBTJ0jwqToUydam11I8plrUptZL6pDjL-Y5zro2pnhYnlWhqIXRzWvy69rEHz-YEIdvkdnOckEEAf59dZnFk8xbZjJS3Wx9TRLoHLAd8uLoAswsb1oOdMbllYu8xL350czw-bWEMDkNG9l0L5sJDy13CjMHifsRPSC4umaFHO6cY2BBDTJlkDDQ6TTTFH5NgLZLMlJ8VT0bwGZ8fzvPi9uOH26tP5c2X689XlzelVW07l6PpZS-t7K3iSlRSt7wWQ61xAA4WGqMq2qCCtufDKOSgBKrKjFpwDUL18rx4s7bdpfhjwTx3k8sWvYeApLprq7pptKobIl__Q97FJZF4goSRVV2pmiCxQjbFnBOO3S65CdJ9J3i3t7Vbbe1IVLe3tTNU8_LQeOknHP5W_PGRgIsDANmCH8lN6_KR07WQmrfEVSuXKRU2mI4K_zf91Vo0Quxgk6jxt6-0M0UfSrWNbuVvuZ3IBA</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Peng, Xue</creator><creator>Yamamoto, Shogo</creator><creator>Vertès, Alain A</creator><creator>Keresztes, Gabor</creator><creator>Inatomi, Ken-ichi</creator><creator>Inui, Masayuki</creator><creator>Yukawa, Hideaki</creator><general>Springer-Verlag</general><general>Springer</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope></search><sort><creationdate>20120201</creationdate><title>Global transcriptome analysis of the tetrachloroethene-dechlorinating bacterium Desulfitobacterium hafniense Y51 in the presence of various electron donors and terminal electron acceptors</title><author>Peng, Xue ; Yamamoto, Shogo ; Vertès, Alain A ; Keresztes, Gabor ; Inatomi, Ken-ichi ; Inui, Masayuki ; Yukawa, Hideaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-f7b3b3c3bc40412359061d65eda0aca87420114a9b0df13d41e427f5105a14b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>acetyl coenzyme A</topic><topic>Analysis</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biodegradation, Environmental</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Bioremediation</topic><topic>Biotechnology</topic><topic>Chemical engineering</topic><topic>Chlorophenol</topic><topic>dehalogenation</topic><topic>Desulfitobacterium - genetics</topic><topic>Desulfitobacterium - growth & development</topic><topic>Desulfitobacterium - metabolism</topic><topic>Desulfitobacterium hafniense</topic><topic>Detoxification</topic><topic>dimethyl sulfoxide</topic><topic>DNA probes</topic><topic>Electrons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Profiling</topic><topic>gene expression regulation</topic><topic>Genes</topic><topic>Genetic Engineering</topic><topic>Genomes</topic><topic>Halogenated compounds</topic><topic>Halogenation</topic><topic>Hydrogen</topic><topic>Inorganic Chemistry</topic><topic>Iron-Sulfur Proteins - genetics</topic><topic>Iron-Sulfur Proteins - metabolism</topic><topic>Laboratories</topic><topic>Life Sciences</topic><topic>microarray technology</topic><topic>Microbiology</topic><topic>Nitrates</topic><topic>Original Paper</topic><topic>Oxidants - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Oxidoreductases - genetics</topic><topic>Oxidoreductases - metabolism</topic><topic>Oxidoreductases, O-Demethylating - genetics</topic><topic>Oxidoreductases, O-Demethylating - metabolism</topic><topic>Physiology</topic><topic>pollution</topic><topic>Proteins</topic><topic>pyruvate synthase</topic><topic>pyruvic acid</topic><topic>Respiration</topic><topic>Respiratory system</topic><topic>Soil contamination</topic><topic>Studies</topic><topic>Succinate Dehydrogenase - genetics</topic><topic>Succinate Dehydrogenase - metabolism</topic><topic>Tetrachloroethylene</topic><topic>Tetrachloroethylene - metabolism</topic><topic>Transcriptome</topic><topic>transcriptomics</topic><topic>Trichloroethylene - metabolism</topic><topic>Water Pollutants, Chemical - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peng, Xue</creatorcontrib><creatorcontrib>Yamamoto, Shogo</creatorcontrib><creatorcontrib>Vertès, Alain A</creatorcontrib><creatorcontrib>Keresztes, Gabor</creatorcontrib><creatorcontrib>Inatomi, Ken-ichi</creatorcontrib><creatorcontrib>Inui, Masayuki</creatorcontrib><creatorcontrib>Yukawa, Hideaki</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Business Premium Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Journal of industrial microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Xue</au><au>Yamamoto, Shogo</au><au>Vertès, Alain A</au><au>Keresztes, Gabor</au><au>Inatomi, Ken-ichi</au><au>Inui, Masayuki</au><au>Yukawa, Hideaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Global transcriptome analysis of the tetrachloroethene-dechlorinating bacterium Desulfitobacterium hafniense Y51 in the presence of various electron donors and terminal electron acceptors</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><stitle>J Ind Microbiol Biotechnol</stitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>39</volume><issue>2</issue><spage>255</spage><epage>268</epage><pages>255-268</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>Desulfitobacterium hafniense Y51 is a dechlorinating bacterium that encodes an unusually large set of O-demethylase paralogs and specialized respiratory systems including specialized electron donors and acceptors. To use this organism in bioremediation of tetrachloroethene (PCE) or trichloroethene (TCE) pollution, expression patterns of its 5,060 genes were determined under different conditions using 60-mer probes in DNA microarrays. PCE, TCE, fumarate, nitrate, and dimethyl sulfoxide (DMSO) respiration all sustain the growth of strain Y51. Global transcriptome analyses were thus performed using various electron donor and acceptor couples (respectively, pyruvate and either fumarate, TCE, nitrate, or DMSO, and vanillate/fumarate). When TCE is used as terminal electron acceptor, resulting in its detoxification, a series of electron carriers comprising a cytochrome bd-type quinol oxidase (DSY4055-4056), a ferredoxin (DSY1451), and four Fe–S proteins (DSY1626, DSY1629, DSY0733, DSY3309) are upregulated, suggesting that the products of these genes are involved in PCE oxidoreduction. Interestingly, the PCE dehalogenase cluster (pceABCT) is constitutively expressed in the media tested, with pceT being upregulated and pceC downregulated in pyruvate/TCE-containing medium. In addition, another dehalogenation enzyme (DSY1155 coding for a putative chlorophenol reductive dehalogenase), is induced 225-fold in that medium, despite not being involved in PCE respiration. Remarkably since the reducing equivalents formed during pyruvate conversion to acetyl-CoA are channeled to electron acceptors including halogenated compounds, pyruvate induces expression of a pyruvate:ferredoxin oxidoreductase. This study paves the way to understanding the physiology of D. hafniense, optimizing this microbe as a bioremediation agent, and designing bioarray sensors to monitor the presence of dechlorinating organisms in the environment.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21861158</pmid><doi>10.1007/s10295-011-1023-7</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1367-5435 |
| ispartof | Journal of industrial microbiology & biotechnology, 2012-02, Vol.39 (2), p.255-268 |
| issn | 1367-5435 1476-5535 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_926885468 |
| source | Oxford Journals Open Access Collection; MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | acetyl coenzyme A Analysis Animals Bacteria Biochemistry Biodegradation, Environmental Bioinformatics Biological and medical sciences Biomedical and Life Sciences Bioremediation Biotechnology Chemical engineering Chlorophenol dehalogenation Desulfitobacterium - genetics Desulfitobacterium - growth & development Desulfitobacterium - metabolism Desulfitobacterium hafniense Detoxification dimethyl sulfoxide DNA probes Electrons Fundamental and applied biological sciences. Psychology gene expression Gene Expression Profiling gene expression regulation Genes Genetic Engineering Genomes Halogenated compounds Halogenation Hydrogen Inorganic Chemistry Iron-Sulfur Proteins - genetics Iron-Sulfur Proteins - metabolism Laboratories Life Sciences microarray technology Microbiology Nitrates Original Paper Oxidants - metabolism Oxidation-Reduction Oxidoreductases - genetics Oxidoreductases - metabolism Oxidoreductases, O-Demethylating - genetics Oxidoreductases, O-Demethylating - metabolism Physiology pollution Proteins pyruvate synthase pyruvic acid Respiration Respiratory system Soil contamination Studies Succinate Dehydrogenase - genetics Succinate Dehydrogenase - metabolism Tetrachloroethylene Tetrachloroethylene - metabolism Transcriptome transcriptomics Trichloroethylene - metabolism Water Pollutants, Chemical - metabolism |
| title | Global transcriptome analysis of the tetrachloroethene-dechlorinating bacterium Desulfitobacterium hafniense Y51 in the presence of various electron donors and terminal electron acceptors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T15%3A30%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Global%20transcriptome%20analysis%20of%20the%20tetrachloroethene-dechlorinating%20bacterium%20Desulfitobacterium%20hafniense%20Y51%20in%20the%20presence%20of%20various%20electron%20donors%20and%20terminal%20electron%20acceptors&rft.jtitle=Journal%20of%20industrial%20microbiology%20&%20biotechnology&rft.au=Peng,%20Xue&rft.date=2012-02-01&rft.volume=39&rft.issue=2&rft.spage=255&rft.epage=268&rft.pages=255-268&rft.issn=1367-5435&rft.eissn=1476-5535&rft_id=info:doi/10.1007/s10295-011-1023-7&rft_dat=%3Cproquest_cross%3E926885468%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=917326246&rft_id=info:pmid/21861158&rfr_iscdi=true |