Overexpression of tobacco ethylene response factor NtERF3 gene and its homologues from tobacco and rice induces hypersensitive response-like cell death in tobacco
Among plant-specific transcription factors, ethylene response factors (ERFs) comprise one of the largest families. ERF s are unique to the plant kingdom and are considered to have crucial roles in plant response to various biotic and abiotic environmental stresses. Here, we report on the functional...
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| Veröffentlicht in: | Journal of general plant pathology : JGPP 2012-01, Vol.78 (1), p.8-17 |
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| creator | Ogata, Takuya Kida, Yuma Arai, Tomoyuki Kishi, Yasuyuki Manago, Yuki Murai, Masato Matsushita, Yasuhiko |
| description | Among plant-specific transcription factors, ethylene response factors (ERFs) comprise one of the largest families.
ERF
s are unique to the plant kingdom and are considered to have crucial roles in plant response to various biotic and abiotic environmental stresses. Here, we report on the functional analysis of a transcriptional repressor, NtERF3, with regard to cell death associated with a hypersensitive response (HR), a plant-specific resistance reaction against pathogens. Expression of
NtERF3
was upregulated during HR induction by
Tobacco mosaic virus
(TMV) infection in tobacco plants harboring the resistance
N
gene to TMV. Transient overexpression of
NtERF3
by
Agrobacterium
-mediated gene delivery induced HR-like cell death in tobacco, associated with the production of reactive oxygen species and ion leakage. Deletion of the ERF-associated amphiphilic repression (EAR) motif from NtERF3 resulted in no induction of cell death, while the deletion had no effect on nuclear localization of the proteins. After virus-mediated gene delivery, similar results also were observed in tobacco without the
N
gene. In addition to NtERF3, other EAR motif-containing ERFs from tobacco,
Nicotiana benthamiana
and rice also induced cell death when overproduced in tobacco plants. The results suggested that many
ERF
genes encoding EAR motif-containing proteins might have the ability to induce cell death when overexpressed. |
| doi_str_mv | 10.1007/s10327-011-0355-5 |
| format | Article |
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ERF
s are unique to the plant kingdom and are considered to have crucial roles in plant response to various biotic and abiotic environmental stresses. Here, we report on the functional analysis of a transcriptional repressor, NtERF3, with regard to cell death associated with a hypersensitive response (HR), a plant-specific resistance reaction against pathogens. Expression of
NtERF3
was upregulated during HR induction by
Tobacco mosaic virus
(TMV) infection in tobacco plants harboring the resistance
N
gene to TMV. Transient overexpression of
NtERF3
by
Agrobacterium
-mediated gene delivery induced HR-like cell death in tobacco, associated with the production of reactive oxygen species and ion leakage. Deletion of the ERF-associated amphiphilic repression (EAR) motif from NtERF3 resulted in no induction of cell death, while the deletion had no effect on nuclear localization of the proteins. After virus-mediated gene delivery, similar results also were observed in tobacco without the
N
gene. In addition to NtERF3, other EAR motif-containing ERFs from tobacco,
Nicotiana benthamiana
and rice also induced cell death when overproduced in tobacco plants. The results suggested that many
ERF
genes encoding EAR motif-containing proteins might have the ability to induce cell death when overexpressed.</description><identifier>ISSN: 1345-2630</identifier><identifier>EISSN: 1610-739X</identifier><identifier>DOI: 10.1007/s10327-011-0355-5</identifier><identifier>CODEN: JGPPBQ</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Agriculture ; Apoptosis ; Biomedical and Life Sciences ; Cell death ; Crop diseases ; Disease resistance ; Ear ; Environmental stress ; Ethylene ; Gene expression ; Gene transfer ; Host Responses ; Hypersensitive response ; Infection ; Leakage ; Life Sciences ; Microbiology ; Mortality ; N gene ; Nicotiana benthamiana ; Oryza sativa ; Pathogens ; Plant diseases ; Plant Pathology ; Plant viruses ; Proteins ; Reactive oxygen species ; Repressors ; Rice ; Tobacco ; Tobacco mosaic virus ; Transcription factors</subject><ispartof>Journal of general plant pathology : JGPP, 2012-01, Vol.78 (1), p.8-17</ispartof><rights>The Author(s) 2011</rights><rights>The Phytopathological Society of Japan and Springer 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-4b7f2f17e5bfbbbab3d7925c8d6932e05fded9cb87928a6272c128fafa44b1bf3</citedby><cites>FETCH-LOGICAL-c485t-4b7f2f17e5bfbbbab3d7925c8d6932e05fded9cb87928a6272c128fafa44b1bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10327-011-0355-5$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10327-011-0355-5$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Ogata, Takuya</creatorcontrib><creatorcontrib>Kida, Yuma</creatorcontrib><creatorcontrib>Arai, Tomoyuki</creatorcontrib><creatorcontrib>Kishi, Yasuyuki</creatorcontrib><creatorcontrib>Manago, Yuki</creatorcontrib><creatorcontrib>Murai, Masato</creatorcontrib><creatorcontrib>Matsushita, Yasuhiko</creatorcontrib><title>Overexpression of tobacco ethylene response factor NtERF3 gene and its homologues from tobacco and rice induces hypersensitive response-like cell death in tobacco</title><title>Journal of general plant pathology : JGPP</title><addtitle>J Gen Plant Pathol</addtitle><description>Among plant-specific transcription factors, ethylene response factors (ERFs) comprise one of the largest families.
ERF
s are unique to the plant kingdom and are considered to have crucial roles in plant response to various biotic and abiotic environmental stresses. Here, we report on the functional analysis of a transcriptional repressor, NtERF3, with regard to cell death associated with a hypersensitive response (HR), a plant-specific resistance reaction against pathogens. Expression of
NtERF3
was upregulated during HR induction by
Tobacco mosaic virus
(TMV) infection in tobacco plants harboring the resistance
N
gene to TMV. Transient overexpression of
NtERF3
by
Agrobacterium
-mediated gene delivery induced HR-like cell death in tobacco, associated with the production of reactive oxygen species and ion leakage. Deletion of the ERF-associated amphiphilic repression (EAR) motif from NtERF3 resulted in no induction of cell death, while the deletion had no effect on nuclear localization of the proteins. After virus-mediated gene delivery, similar results also were observed in tobacco without the
N
gene. In addition to NtERF3, other EAR motif-containing ERFs from tobacco,
Nicotiana benthamiana
and rice also induced cell death when overproduced in tobacco plants. The results suggested that many
ERF
genes encoding EAR motif-containing proteins might have the ability to induce cell death when overexpressed.</description><subject>Agriculture</subject><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Cell death</subject><subject>Crop diseases</subject><subject>Disease resistance</subject><subject>Ear</subject><subject>Environmental stress</subject><subject>Ethylene</subject><subject>Gene expression</subject><subject>Gene transfer</subject><subject>Host Responses</subject><subject>Hypersensitive response</subject><subject>Infection</subject><subject>Leakage</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mortality</subject><subject>N gene</subject><subject>Nicotiana benthamiana</subject><subject>Oryza sativa</subject><subject>Pathogens</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Plant viruses</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Repressors</subject><subject>Rice</subject><subject>Tobacco</subject><subject>Tobacco mosaic virus</subject><subject>Transcription 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of tobacco ethylene response factor NtERF3 gene and its homologues from tobacco and rice induces hypersensitive response-like cell death in tobacco</title><author>Ogata, Takuya ; Kida, Yuma ; Arai, Tomoyuki ; Kishi, Yasuyuki ; Manago, Yuki ; Murai, Masato ; Matsushita, Yasuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-4b7f2f17e5bfbbbab3d7925c8d6932e05fded9cb87928a6272c128fafa44b1bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agriculture</topic><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Cell death</topic><topic>Crop diseases</topic><topic>Disease resistance</topic><topic>Ear</topic><topic>Environmental stress</topic><topic>Ethylene</topic><topic>Gene expression</topic><topic>Gene transfer</topic><topic>Host Responses</topic><topic>Hypersensitive response</topic><topic>Infection</topic><topic>Leakage</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Mortality</topic><topic>N gene</topic><topic>Nicotiana benthamiana</topic><topic>Oryza sativa</topic><topic>Pathogens</topic><topic>Plant diseases</topic><topic>Plant Pathology</topic><topic>Plant viruses</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Repressors</topic><topic>Rice</topic><topic>Tobacco</topic><topic>Tobacco mosaic virus</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ogata, Takuya</creatorcontrib><creatorcontrib>Kida, Yuma</creatorcontrib><creatorcontrib>Arai, Tomoyuki</creatorcontrib><creatorcontrib>Kishi, Yasuyuki</creatorcontrib><creatorcontrib>Manago, Yuki</creatorcontrib><creatorcontrib>Murai, Masato</creatorcontrib><creatorcontrib>Matsushita, Yasuhiko</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogata, Takuya</au><au>Kida, Yuma</au><au>Arai, Tomoyuki</au><au>Kishi, Yasuyuki</au><au>Manago, Yuki</au><au>Murai, Masato</au><au>Matsushita, Yasuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of tobacco ethylene response factor NtERF3 gene and its homologues from tobacco and rice induces hypersensitive response-like cell death in tobacco</atitle><jtitle>Journal of general plant pathology : JGPP</jtitle><stitle>J Gen Plant Pathol</stitle><date>2012-01-01</date><risdate>2012</risdate><volume>78</volume><issue>1</issue><spage>8</spage><epage>17</epage><pages>8-17</pages><issn>1345-2630</issn><eissn>1610-739X</eissn><coden>JGPPBQ</coden><abstract>Among plant-specific transcription factors, ethylene response factors (ERFs) comprise one of the largest families.
ERF
s are unique to the plant kingdom and are considered to have crucial roles in plant response to various biotic and abiotic environmental stresses. Here, we report on the functional analysis of a transcriptional repressor, NtERF3, with regard to cell death associated with a hypersensitive response (HR), a plant-specific resistance reaction against pathogens. Expression of
NtERF3
was upregulated during HR induction by
Tobacco mosaic virus
(TMV) infection in tobacco plants harboring the resistance
N
gene to TMV. Transient overexpression of
NtERF3
by
Agrobacterium
-mediated gene delivery induced HR-like cell death in tobacco, associated with the production of reactive oxygen species and ion leakage. Deletion of the ERF-associated amphiphilic repression (EAR) motif from NtERF3 resulted in no induction of cell death, while the deletion had no effect on nuclear localization of the proteins. After virus-mediated gene delivery, similar results also were observed in tobacco without the
N
gene. In addition to NtERF3, other EAR motif-containing ERFs from tobacco,
Nicotiana benthamiana
and rice also induced cell death when overproduced in tobacco plants. The results suggested that many
ERF
genes encoding EAR motif-containing proteins might have the ability to induce cell death when overexpressed.</abstract><cop>Japan</cop><pub>Springer Japan</pub><doi>10.1007/s10327-011-0355-5</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agriculture Apoptosis Biomedical and Life Sciences Cell death Crop diseases Disease resistance Ear Environmental stress Ethylene Gene expression Gene transfer Host Responses Hypersensitive response Infection Leakage Life Sciences Microbiology Mortality N gene Nicotiana benthamiana Oryza sativa Pathogens Plant diseases Plant Pathology Plant viruses Proteins Reactive oxygen species Repressors Rice Tobacco Tobacco mosaic virus Transcription factors |
| title | Overexpression of tobacco ethylene response factor NtERF3 gene and its homologues from tobacco and rice induces hypersensitive response-like cell death in tobacco |
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