Comprehensive characterization of two different Nicotiana tabacum cell lines leads to doubled GFP and HA protein production by media optimization

For over two decades, plant cell cultures have been a promising research platform to express recombinant and therapeutic proteins such as hormones, growth factors, full-size antibodies and antigens. Chosen as a good host for manufacturing recombinant proteins, the Nicotiana tabacum L. cv. Bright Yel...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2012-02, Vol.113 (2), p.242-248
Hauptverfasser:	Ullisch, David A., Müller, Christina A., Maibaum, Sabrina, Kirchhoff, Janina, Schiermeyer, Andreas, Schillberg, Stefan, Roberts, Jean L., Treffenfeldt, Wiltrud, Büchs, Jochen
Format:	Artikel
Sprache:	eng
Schlagworte:	antibodies antigens Biological and medical sciences biopharmaceuticals Biotechnology BY-2 cells cell culture cell growth containers Culture Media - chemistry Fundamental and applied biological sciences. Psychology glycoproteins Green Fluorescent Proteins - biosynthesis Green Fluorescent Proteins - genetics growth factors Hemagglutinin Glycoproteins, Influenza Virus - biosynthesis Hemagglutinin Glycoproteins, Influenza Virus - genetics hemagglutinins hormones influenza Influenza hemagglutinin manufacturing Media optimization monitoring Nicotiana - cytology Nicotiana - genetics Nicotiana - growth & development Nicotiana - metabolism Nicotiana tabacum Nitrogen source oxygen Oxygen transfer rate Plant cell culture Plants, Genetically Modified - cytology Plants, Genetically Modified - growth & development Plants, Genetically Modified - metabolism protein content Quaternary Ammonium Compounds - analysis recombinant proteins Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - metabolism respiration activity monitoring system (RAMOS) Shake flasks tobacco transgenic plants
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