Quantification of heat shock protein mRNA expression in warm and cold anoxic turtles ( Trachemys scripta) using an external RNA control for normalization

The mRNA expression of heat-shock protein 90 (HSP90) and heat-shock cognate 70 (HSC70) was examined in cardiac chambers and telencephalon of warm- (21 °C) and cold-acclimated (5 °C) turtles ( Trachemys scripta) exposed to normoxia, prolonged anoxia or anoxia followed by reoxygenation. Additionally,...

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Veröffentlicht in:	Comparative biochemistry and physiology. Part D, Genomics & proteomics Genomics & proteomics, 2012-03, Vol.7 (1), p.59-72
Hauptverfasser:	Stecyk, Jonathan A.W., Couturier, Christine S., Fagernes, Cathrine E., Ellefsen, Stian, Nilsson, Göran E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acclimatization Animals Anoxia Body Temperature Brain Brain - metabolism Gene Expression Regulation Heart HSC70 HSC70 Heat-Shock Proteins - genetics HSP90 HSP90 Heat-Shock Proteins - genetics Hypoxia - genetics Hypoxia - metabolism Internal RNA controls mw2060 Myocardium - metabolism Oxygen - metabolism RNA, Messenger - analysis RNA, Messenger - genetics Temperature Trachemys scripta Turtles - genetics Turtles - metabolism
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