Formation and characterization of pDNA-loaded alginate microspheres for oral administration in mice

Alginate, a natural polysaccharide, was explored in this study as an oral delivery vehicle of a mammalian expression vector into the murine intestinal mucosa. Alginate microspheres were produced through water-in-oil (W/O) emulsification method. Average diameter sizes of microspheres were 46.88μm±3.0...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2012-02, Vol.113 (2), p.133-140
Hauptverfasser:	Nograles, Nadine, Abdullah, Syahril, Shamsudin, Mariana Nor, Billa, Nashiru, Rosli, Rozita
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Oral Alginate microspheres Alginates - chemistry Alginic acid Animals Biological and medical sciences Biotechnology DNA - administration & dosage DNA vaccine emulsifying Encapsulation flow cytometry Fundamental and applied biological sciences. Psychology gene transfer Gene Transfer Techniques Glucuronic Acid - chemistry green fluorescent protein Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics Hexuronic Acids - chemistry intestinal mucosa Mice Microspheres oral administration Oral delivery Particle Size plasmids Plasmids - genetics polysaccharides reporter genes shrinkage Tissue Distribution vaccination W/O emulsion
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Zusammenfassung:	Alginate, a natural polysaccharide, was explored in this study as an oral delivery vehicle of a mammalian expression vector into the murine intestinal mucosa. Alginate microspheres were produced through water-in-oil (W/O) emulsification method. Average diameter sizes of microspheres were 46.88μm±3.07μm with significant size reduction upon utilization of 1.0% Span80. Plasmid DNA (pDNA) carrying green fluorescent protein reporter gene (GFP), pVAX-GFP, was encapsulated within microspheres at efficiencies of 72.9 to 74.4%, carrying maximum load of 6μg pDNA. Alginate microspheres demonstrated shrinkage in pH 1.2 and swelling in pH 9.0 with pDNA release about twice the amount released in acidic environment. Oral delivery of pVAX-GFP loaded-microspheres, at 50μg, 100μg and 150μg dose, was performed on BALB/c mice. Tissue biodistribution, investigated through flow cytometric analysis, demonstrated GFP positive intestinal cells (
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2011.10.003