Graphene oxide-protected DNA probes for multiplex microRNA analysis in complex biological samples based on a cyclic enzymatic amplification method

Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples. By using the quenching nature of graphene oxide for multiple fluorophores, this method can distinguish highly similar miRNA sequences a...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2012-01, Vol.48 (2), p.194-196
Hauptverfasser:	Cui, Liang, Lin, Xiaoyan, Lin, Ninghang, Song, Yanling, Zhu, Zhi, Chen, Xi, Yang, Chaoyong James
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensing Techniques - methods Chemical communication Deoxyribonuclease I - metabolism DNA Probes - chemistry Fluorescent Dyes - chemistry Graphite - chemistry Humans MicroRNAs - analysis MicroRNAs - metabolism Oxides - chemistry Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples. By using the quenching nature of graphene oxide for multiple fluorophores, this method can distinguish highly similar miRNA sequences and detect them simultaneously.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c1cc15412e