Purification of Small Interfering RNA Using Nondenaturing Anion-Exchange Chromatography

A manufacturing and purification process for duplex oligonucleotides was established, which shortens and simplifies currently used procedures, yielding a product of higher purity. The reported procedure is based on nondenaturing anion-exchange (AEX) chromatography, which is performed on the annealed...

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Veröffentlicht in:	Nucleic acid therapeutics 2011-12, Vol.21 (6), p.383-393
Hauptverfasser:	Noll, Bernhard, Seiffert, Stephan, Hertel, Frank, Debelak, Harald, Hadwiger, Philipp, Vornlocher, Hans-Peter, Roehl, Ingo
Format:	Artikel
Sprache:	eng
Schlagworte:	Anion Exchange Resins - chemistry Chromatography Chromatography, Ion Exchange - methods Mass Spectrometry - methods Molecular biology Oligonucleotides - chemical synthesis Oligonucleotides - chemistry Original Articles Resins Ribonucleic acid RNA RNA, Small Interfering - analysis RNA, Small Interfering - chemistry RNA, Small Interfering - isolation & purification
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