Development and characterization of a monoclonal antibody against porcine CD205
The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designat...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2012-03, Vol.146 (1), p.74-80 |
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| container_title | Veterinary immunology and immunopathology |
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| creator | Flores-Mendoza, Lilian Velazquez, Carlos Bray, Jocelyn Njongmeta, Leo Mwangi, Waithaka Hernández, Jesús |
| description | The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of ∼200kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1% and 34.1% of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6% of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20% were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70% of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses. |
| doi_str_mv | 10.1016/j.vetimm.2012.01.011 |
| format | Article |
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To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of ∼200kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1% and 34.1% of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6% of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20% were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70% of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2012.01.011</identifier><identifier>PMID: 22348805</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antigens, CD - immunology ; C-type lectin receptors ; CD205 ; Cell Line ; Dendritic cells ; Dendritic Cells - immunology ; Lectins, C-Type - immunology ; Mice ; Mice, Inbred C3H ; Minor Histocompatibility Antigens ; Porcine ; Receptors, Cell Surface - immunology ; Swine</subject><ispartof>Veterinary immunology and immunopathology, 2012-03, Vol.146 (1), p.74-80</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-cdff557d76bf66796d31035fc803fa169a00351d5dc00c70cfbddee70dc1a89f3</citedby><cites>FETCH-LOGICAL-c361t-cdff557d76bf66796d31035fc803fa169a00351d5dc00c70cfbddee70dc1a89f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165242712000141$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22348805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flores-Mendoza, Lilian</creatorcontrib><creatorcontrib>Velazquez, Carlos</creatorcontrib><creatorcontrib>Bray, Jocelyn</creatorcontrib><creatorcontrib>Njongmeta, Leo</creatorcontrib><creatorcontrib>Mwangi, Waithaka</creatorcontrib><creatorcontrib>Hernández, Jesús</creatorcontrib><title>Development and characterization of a monoclonal antibody against porcine CD205</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of ∼200kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1% and 34.1% of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6% of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20% were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70% of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, CD - immunology</subject><subject>C-type lectin receptors</subject><subject>CD205</subject><subject>Cell Line</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Lectins, C-Type - immunology</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Minor Histocompatibility Antigens</subject><subject>Porcine</subject><subject>Receptors, Cell Surface - immunology</subject><subject>Swine</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLQzEQhYMoWh__QOTuXN06SZr72AhSnyC40XVIk4mm3JvUJC3orzdSdSkcGAa-M4c5hJxSmFKgzcVyusHsxnHKgLIp0CK6Qya0a3nNBJ_tkknBRM1mrD0ghyktAUD0XbdPDhjjs64DMSFP17jBIaxG9LlS3lT6TUWlM0b3qbILvgq2UtUYfNBD8GooUHaLYD4q9aqcT7lahaidx2p-zUAckz2rhoQnP_OIvNzePM_v68enu4f51WOteUNzrY21QrSmbRa2adq-MZwCF1Z3wK2iTa-grNQIowF0C9oujEFswWiqut7yI3K-vbuK4X2NKcvRJY3DoDyGdZI9a7q2570o5GxL6hhSimjlKrpRxQ9JQX43KZdy26T8blICLaLFdvYTsF6MaP5Mv9UV4HILYHlz4zDKpB16jcZF1Fma4P5P-AKKvYck</recordid><startdate>20120315</startdate><enddate>20120315</enddate><creator>Flores-Mendoza, Lilian</creator><creator>Velazquez, Carlos</creator><creator>Bray, Jocelyn</creator><creator>Njongmeta, Leo</creator><creator>Mwangi, Waithaka</creator><creator>Hernández, Jesús</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120315</creationdate><title>Development and characterization of a monoclonal antibody against porcine CD205</title><author>Flores-Mendoza, Lilian ; Velazquez, Carlos ; Bray, Jocelyn ; Njongmeta, Leo ; Mwangi, Waithaka ; Hernández, Jesús</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-cdff557d76bf66796d31035fc803fa169a00351d5dc00c70cfbddee70dc1a89f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, CD - immunology</topic><topic>C-type lectin receptors</topic><topic>CD205</topic><topic>Cell Line</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Lectins, C-Type - immunology</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Minor Histocompatibility Antigens</topic><topic>Porcine</topic><topic>Receptors, Cell Surface - immunology</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flores-Mendoza, Lilian</creatorcontrib><creatorcontrib>Velazquez, Carlos</creatorcontrib><creatorcontrib>Bray, Jocelyn</creatorcontrib><creatorcontrib>Njongmeta, Leo</creatorcontrib><creatorcontrib>Mwangi, Waithaka</creatorcontrib><creatorcontrib>Hernández, Jesús</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flores-Mendoza, Lilian</au><au>Velazquez, Carlos</au><au>Bray, Jocelyn</au><au>Njongmeta, Leo</au><au>Mwangi, Waithaka</au><au>Hernández, Jesús</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and characterization of a monoclonal antibody against porcine CD205</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2012-03-15</date><risdate>2012</risdate><volume>146</volume><issue>1</issue><spage>74</spage><epage>80</epage><pages>74-80</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. 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| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antigens, CD - immunology C-type lectin receptors CD205 Cell Line Dendritic cells Dendritic Cells - immunology Lectins, C-Type - immunology Mice Mice, Inbred C3H Minor Histocompatibility Antigens Porcine Receptors, Cell Surface - immunology Swine |
| title | Development and characterization of a monoclonal antibody against porcine CD205 |
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