Decreased expression of repulsive guidance molecule member A by DNA methylation in colorectal cancer is related to tumor progression
Previous studies have shown decreased expression of repulsive guidance molecule member A (RGMa) in colorectal cancer. However, the relationship between the expression levels and promoter DNA methylation status of RGMa and the clinical characteristics of colorectal cancer has not been previously repo...
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| Veröffentlicht in: | Oncology reports 2012-05, Vol.27 (5), p.1653-1659 |
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| creator | ZHAO, Zhi-Wei LIAN, Wen-Jing CHEN, Guo-Qing ZHOU, Hong-Ying WANG, Gui-Ming XIA CAO YANG, Hui-Jun HOU, Yi-Ping |
| description | Previous studies have shown decreased expression of repulsive guidance molecule member A (RGMa) in colorectal cancer. However, the relationship between the expression levels and promoter DNA methylation status of RGMa and the clinical characteristics of colorectal cancer has not been previously reported. Here, we investigated the expression of RGMa by immunohistochemistry, real-time PCR and western blotting and analyzed the methylation status of the RGMa promoter using Sequenom's MassARRAY platform in colorectal cancer tissues and adjacent normal colorectal tissues. The results showed that RGMa expression was decreased in cancer tissues compared with adjacent normal tissues (p |
| doi_str_mv | 10.3892/or.2012.1693 |
| format | Article |
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However, the relationship between the expression levels and promoter DNA methylation status of RGMa and the clinical characteristics of colorectal cancer has not been previously reported. Here, we investigated the expression of RGMa by immunohistochemistry, real-time PCR and western blotting and analyzed the methylation status of the RGMa promoter using Sequenom's MassARRAY platform in colorectal cancer tissues and adjacent normal colorectal tissues. The results showed that RGMa expression was decreased in cancer tissues compared with adjacent normal tissues (p<0.01). Furthermore, a tendency for decreased expression in tumor tissues was observed from Dukes' stage A to stage D (p<0.01). In addition, significantly higher levels of hypermethylation in promoter regions of RGMa were observed in colorectal cancer tissues, compared with those in adjacent normal colorectal tissues (p<0.01). Moreover, the methylation levels of RGMa in tumor tissues were significantly increased in Dukes' stage C and D compared with Dukes' stage A and B (p<0.01). Our results indicate that RGMa expression and promoter methylation status are closely related to colorectal cancer genesis and progression. Determination of the expression level and methylation frequency of RGMa in colorectal cancer tissues may have benefit for early diagnosis and for evaluating patient prognosis.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2012.1693</identifier><identifier>PMID: 22367090</identifier><language>eng</language><publisher>Athens: Spandidos</publisher><subject>Adult ; Aged ; Base Sequence ; Biological and medical sciences ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Disease Progression ; DNA Methylation ; Epigenesis, Genetic ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Expression Regulation, Neoplastic ; GPI-Linked Proteins - genetics ; GPI-Linked Proteins - metabolism ; Humans ; Male ; Medical sciences ; Middle Aged ; Molecular Sequence Data ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Promoter Regions, Genetic ; Stomach. Duodenum. Small intestine. Colon. Rectum. 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However, the relationship between the expression levels and promoter DNA methylation status of RGMa and the clinical characteristics of colorectal cancer has not been previously reported. Here, we investigated the expression of RGMa by immunohistochemistry, real-time PCR and western blotting and analyzed the methylation status of the RGMa promoter using Sequenom's MassARRAY platform in colorectal cancer tissues and adjacent normal colorectal tissues. The results showed that RGMa expression was decreased in cancer tissues compared with adjacent normal tissues (p<0.01). Furthermore, a tendency for decreased expression in tumor tissues was observed from Dukes' stage A to stage D (p<0.01). In addition, significantly higher levels of hypermethylation in promoter regions of RGMa were observed in colorectal cancer tissues, compared with those in adjacent normal colorectal tissues (p<0.01). Moreover, the methylation levels of RGMa in tumor tissues were significantly increased in Dukes' stage C and D compared with Dukes' stage A and B (p<0.01). Our results indicate that RGMa expression and promoter methylation status are closely related to colorectal cancer genesis and progression. Determination of the expression level and methylation frequency of RGMa in colorectal cancer tissues may have benefit for early diagnosis and for evaluating patient prognosis.</description><subject>Adult</subject><subject>Aged</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Disease Progression</subject><subject>DNA Methylation</subject><subject>Epigenesis, Genetic</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>GPI-Linked Proteins - genetics</subject><subject>GPI-Linked Proteins - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. 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Liver. Pancreas. Abdomen</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>GPI-Linked Proteins - genetics</topic><topic>GPI-Linked Proteins - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHAO, Zhi-Wei</creatorcontrib><creatorcontrib>LIAN, Wen-Jing</creatorcontrib><creatorcontrib>CHEN, Guo-Qing</creatorcontrib><creatorcontrib>ZHOU, Hong-Ying</creatorcontrib><creatorcontrib>WANG, Gui-Ming</creatorcontrib><creatorcontrib>XIA CAO</creatorcontrib><creatorcontrib>YANG, Hui-Jun</creatorcontrib><creatorcontrib>HOU, Yi-Ping</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZHAO, Zhi-Wei</au><au>LIAN, Wen-Jing</au><au>CHEN, Guo-Qing</au><au>ZHOU, Hong-Ying</au><au>WANG, Gui-Ming</au><au>XIA CAO</au><au>YANG, Hui-Jun</au><au>HOU, Yi-Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased expression of repulsive guidance molecule member A by DNA methylation in colorectal cancer is related to tumor progression</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>27</volume><issue>5</issue><spage>1653</spage><epage>1659</epage><pages>1653-1659</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>Previous studies have shown decreased expression of repulsive guidance molecule member A (RGMa) in colorectal cancer. However, the relationship between the expression levels and promoter DNA methylation status of RGMa and the clinical characteristics of colorectal cancer has not been previously reported. Here, we investigated the expression of RGMa by immunohistochemistry, real-time PCR and western blotting and analyzed the methylation status of the RGMa promoter using Sequenom's MassARRAY platform in colorectal cancer tissues and adjacent normal colorectal tissues. The results showed that RGMa expression was decreased in cancer tissues compared with adjacent normal tissues (p<0.01). Furthermore, a tendency for decreased expression in tumor tissues was observed from Dukes' stage A to stage D (p<0.01). In addition, significantly higher levels of hypermethylation in promoter regions of RGMa were observed in colorectal cancer tissues, compared with those in adjacent normal colorectal tissues (p<0.01). Moreover, the methylation levels of RGMa in tumor tissues were significantly increased in Dukes' stage C and D compared with Dukes' stage A and B (p<0.01). Our results indicate that RGMa expression and promoter methylation status are closely related to colorectal cancer genesis and progression. Determination of the expression level and methylation frequency of RGMa in colorectal cancer tissues may have benefit for early diagnosis and for evaluating patient prognosis.</abstract><cop>Athens</cop><pub>Spandidos</pub><pmid>22367090</pmid><doi>10.3892/or.2012.1693</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Base Sequence Biological and medical sciences Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Disease Progression DNA Methylation Epigenesis, Genetic Female Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Regulation, Neoplastic GPI-Linked Proteins - genetics GPI-Linked Proteins - metabolism Humans Male Medical sciences Middle Aged Molecular Sequence Data Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Promoter Regions, Genetic Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumors |
| title | Decreased expression of repulsive guidance molecule member A by DNA methylation in colorectal cancer is related to tumor progression |
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