Apolipoprotein(a) isoforms immunoblotting detection: comparative study of two methods
This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel elect...
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| Veröffentlicht in: | Annales de biologie clinique (Paris) 2012-01, Vol.70 (1), p.13-17 |
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| container_title | Annales de biologie clinique (Paris) |
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| creator | Edjème-Aké, Angèle Garnotel, Roselyne Vallé-Polneau, Sandrine Ahiboh, Hugues Hauhouot-Attoungbré, Marie Laure Monnet, Dagui Gillery, Philippe |
| description | This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting using enzymatic colorimetry or chemiluminescence. Within-run precision was comprised between 4.9% and 9.2% for colorimetry and between 2.9% and 4.6% for chemiluminescence. Both methods have detected apo(a) isoforms in all patients, even when lipoprotein(a) concentrations were under detection limit (0.02 g/L). The two methods were significantly correlated (r = 0.96 to 0.98, p |
| doi_str_mv | 10.1684/abc.2011.0651 |
| format | Article |
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Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting using enzymatic colorimetry or chemiluminescence. Within-run precision was comprised between 4.9% and 9.2% for colorimetry and between 2.9% and 4.6% for chemiluminescence. Both methods have detected apo(a) isoforms in all patients, even when lipoprotein(a) concentrations were under detection limit (0.02 g/L). The two methods were significantly correlated (r = 0.96 to 0.98, p<0.0001). Even though the chemiluminescence method exhibited better performances than the colorimetric method, both techniques could be used indifferently.</description><identifier>ISSN: 0003-3898</identifier><identifier>DOI: 10.1684/abc.2011.0651</identifier><identifier>PMID: 22294137</identifier><language>fre</language><publisher>France</publisher><subject>Adolescent ; Adult ; Apoprotein(a) - analysis ; Apoprotein(a) - blood ; Apoprotein(a) - metabolism ; Blood Donors ; Colorimetry - methods ; Cote d'Ivoire ; Electrophoresis, Polyacrylamide Gel ; Humans ; Immunoblotting - methods ; Luminescent Measurements - methods ; Middle Aged ; Molecular Weight ; Protein Isoforms - analysis ; Protein Isoforms - metabolism ; Sensitivity and Specificity ; Young Adult</subject><ispartof>Annales de biologie clinique (Paris), 2012-01, Vol.70 (1), p.13-17</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22294137$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Edjème-Aké, Angèle</creatorcontrib><creatorcontrib>Garnotel, Roselyne</creatorcontrib><creatorcontrib>Vallé-Polneau, Sandrine</creatorcontrib><creatorcontrib>Ahiboh, Hugues</creatorcontrib><creatorcontrib>Hauhouot-Attoungbré, Marie Laure</creatorcontrib><creatorcontrib>Monnet, Dagui</creatorcontrib><creatorcontrib>Gillery, Philippe</creatorcontrib><title>Apolipoprotein(a) isoforms immunoblotting detection: comparative study of two methods</title><title>Annales de biologie clinique (Paris)</title><addtitle>Ann Biol Clin (Paris)</addtitle><description>This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. 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Even though the chemiluminescence method exhibited better performances than the colorimetric method, both techniques could be used indifferently.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Apoprotein(a) - analysis</subject><subject>Apoprotein(a) - blood</subject><subject>Apoprotein(a) - metabolism</subject><subject>Blood Donors</subject><subject>Colorimetry - methods</subject><subject>Cote d'Ivoire</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Humans</subject><subject>Immunoblotting - methods</subject><subject>Luminescent Measurements - methods</subject><subject>Middle Aged</subject><subject>Molecular Weight</subject><subject>Protein Isoforms - analysis</subject><subject>Protein Isoforms - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Young Adult</subject><issn>0003-3898</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kLtOwzAART2AaCmMrMgbMCT4HZutqnhJlVjKHDm2A0ZxHGKHqn9PJMp0l6OjowvAFUYlFpLd68aUBGFcIsHxCVgihGhBpZILcJ7SF0KYSErPwIIQohim1RK8r4fY-SEOY8zO97f6DvoU2ziGBH0IUx-bLubs-w9oXXYm-9g_QBPDoEed_Y-DKU_2AGML8z7C4PJntOkCnLa6S-7yuCuwe3rcbV6K7dvz62a9LQbMUC5aI5TBXBHBK0EJw4xZRhjiUjAlrMCKWYk4F1qa1jZGydYZzqilnCjk6Arc_Gnn-u_JpVwHn4zrOt27OKV6FgvGqkrN5PWRnJrgbD2MPujxUP8fQX8BCPFd-A</recordid><startdate>201201</startdate><enddate>201201</enddate><creator>Edjème-Aké, Angèle</creator><creator>Garnotel, Roselyne</creator><creator>Vallé-Polneau, Sandrine</creator><creator>Ahiboh, Hugues</creator><creator>Hauhouot-Attoungbré, Marie Laure</creator><creator>Monnet, Dagui</creator><creator>Gillery, Philippe</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201201</creationdate><title>Apolipoprotein(a) isoforms immunoblotting detection: comparative study of two methods</title><author>Edjème-Aké, Angèle ; Garnotel, Roselyne ; Vallé-Polneau, Sandrine ; Ahiboh, Hugues ; Hauhouot-Attoungbré, Marie Laure ; Monnet, Dagui ; Gillery, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p140t-fc69c15926576324144d4240586496d6194d80556a8cfdbc98fec543d35290e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>fre</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Apoprotein(a) - analysis</topic><topic>Apoprotein(a) - blood</topic><topic>Apoprotein(a) - metabolism</topic><topic>Blood Donors</topic><topic>Colorimetry - methods</topic><topic>Cote d'Ivoire</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Humans</topic><topic>Immunoblotting - methods</topic><topic>Luminescent Measurements - methods</topic><topic>Middle Aged</topic><topic>Molecular Weight</topic><topic>Protein Isoforms - analysis</topic><topic>Protein Isoforms - metabolism</topic><topic>Sensitivity and Specificity</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Edjème-Aké, Angèle</creatorcontrib><creatorcontrib>Garnotel, Roselyne</creatorcontrib><creatorcontrib>Vallé-Polneau, Sandrine</creatorcontrib><creatorcontrib>Ahiboh, Hugues</creatorcontrib><creatorcontrib>Hauhouot-Attoungbré, Marie Laure</creatorcontrib><creatorcontrib>Monnet, Dagui</creatorcontrib><creatorcontrib>Gillery, Philippe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Annales de biologie clinique (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Edjème-Aké, Angèle</au><au>Garnotel, Roselyne</au><au>Vallé-Polneau, Sandrine</au><au>Ahiboh, Hugues</au><au>Hauhouot-Attoungbré, Marie Laure</au><au>Monnet, Dagui</au><au>Gillery, Philippe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apolipoprotein(a) isoforms immunoblotting detection: comparative study of two methods</atitle><jtitle>Annales de biologie clinique (Paris)</jtitle><addtitle>Ann Biol Clin (Paris)</addtitle><date>2012-01</date><risdate>2012</risdate><volume>70</volume><issue>1</issue><spage>13</spage><epage>17</epage><pages>13-17</pages><issn>0003-3898</issn><abstract>This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting using enzymatic colorimetry or chemiluminescence. Within-run precision was comprised between 4.9% and 9.2% for colorimetry and between 2.9% and 4.6% for chemiluminescence. Both methods have detected apo(a) isoforms in all patients, even when lipoprotein(a) concentrations were under detection limit (0.02 g/L). The two methods were significantly correlated (r = 0.96 to 0.98, p<0.0001). Even though the chemiluminescence method exhibited better performances than the colorimetric method, both techniques could be used indifferently.</abstract><cop>France</cop><pmid>22294137</pmid><doi>10.1684/abc.2011.0651</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Free E-Journal (出版社公開部分のみ） |
| subjects | Adolescent Adult Apoprotein(a) - analysis Apoprotein(a) - blood Apoprotein(a) - metabolism Blood Donors Colorimetry - methods Cote d'Ivoire Electrophoresis, Polyacrylamide Gel Humans Immunoblotting - methods Luminescent Measurements - methods Middle Aged Molecular Weight Protein Isoforms - analysis Protein Isoforms - metabolism Sensitivity and Specificity Young Adult |
| title | Apolipoprotein(a) isoforms immunoblotting detection: comparative study of two methods |
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