Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.)
Late-embryogenesis abundant (LEA) proteins have been reported to be closely correlated with the acquisition of desiccation tolerance during seed development and response of plant to drought, salinity, and freezing, etc. In this study, a LEA gene, CarLEA4 (GenBank accession no. GU247511), was isolate...
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| Veröffentlicht in: | Molecular biology reports 2012-04, Vol.39 (4), p.3565-3572 |
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| creator | Gu, Hanyan Jia, Yuying Wang, Xiansheng Chen, Quanjia Shi, Shubing Ma, Lin Zhang, Jusong Zhang, Hua Ma, Hao |
| description | Late-embryogenesis abundant (LEA) proteins have been reported to be closely correlated with the acquisition of desiccation tolerance during seed development and response of plant to drought, salinity, and freezing, etc. In this study, a
LEA
gene,
CarLEA4
(GenBank accession no. GU247511), was isolated from chickpea based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol (PEG).
CarLEA4
contained two exons and one intron within genomic DNA sequence and encoded a putative polypeptide of 152 amino acids. CarLEA4 had a conserved pfam domain, and showed high similarity to the group 4 LEA proteins in secondary structure. It was localized in the nucleus. The transcripts of
CarLEA4
were detected in many chickpea organs including seedling leaves, stems, roots, flowers, young pods, and young seeds.
CarLEA4
was inhibited by leaf age and showed expression changes in expression during seed development, pod development and germination. Furthermore, the expression of
CarLEA4
was strongly induced by drought, salt, heat, cold, ABA, IAA, GA
3
and MeJA. Our results suggest that
CarLEA4
encodes a protein of LEA group 4 and may be involved in various plant developmental processes and abiotic stress responses. |
| doi_str_mv | 10.1007/s11033-011-1130-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_926643095</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2602633831</sourcerecordid><originalsourceid>FETCH-LOGICAL-c370t-3d1d41b22f0450719c186caa82dd7e46dd358d77c937057cde13877e7038bd6c3</originalsourceid><addsrcrecordid>eNp1kLFu2zAQhomiRey4eYAuAdEl6SD3TqREaTQMJw1goEs7EzR5SulYkkNKQ_r0oaE0AQJ0InD8_v8OH2NfEJYIoL5HRBAiA8QMUUBWfmBzLJTIZK2qj2wOAjCTVYEzdh7jHgAkquKMzXJUOagS5szeOeoG33hrBt933HSO2z8mGDtQ8H-nYd9ww7ebFW9M6w9P_J464msT0kjyJvRtinj7cCTDr9feUuAmeBp8N7Z8u_z2mX1qzCHSxcu7YL9vNr_WP7Ltz9u79WqbWaFgyIRDJ3GX5w3IAhTWFqvSGlPlzimSpXOiqJxStk54oawjFJVSpEBUO1dasWBXU-8x9I8jxUG3Plo6HExH_Rh1nZelFFAXifz6jtz3Y-jScSdIyKLKVYJwgmzoYwzU6GPwrQlPGkGf_OvJv07-9cm_LlPm8qV43LXkXhP_hCcgn4CYvrp7Cm-b_9_6DIwxjXk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>926345827</pqid></control><display><type>article</type><title>Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.)</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Gu, Hanyan ; Jia, Yuying ; Wang, Xiansheng ; Chen, Quanjia ; Shi, Shubing ; Ma, Lin ; Zhang, Jusong ; Zhang, Hua ; Ma, Hao</creator><creatorcontrib>Gu, Hanyan ; Jia, Yuying ; Wang, Xiansheng ; Chen, Quanjia ; Shi, Shubing ; Ma, Lin ; Zhang, Jusong ; Zhang, Hua ; Ma, Hao</creatorcontrib><description>Late-embryogenesis abundant (LEA) proteins have been reported to be closely correlated with the acquisition of desiccation tolerance during seed development and response of plant to drought, salinity, and freezing, etc. In this study, a
LEA
gene,
CarLEA4
(GenBank accession no. GU247511), was isolated from chickpea based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol (PEG).
CarLEA4
contained two exons and one intron within genomic DNA sequence and encoded a putative polypeptide of 152 amino acids. CarLEA4 had a conserved pfam domain, and showed high similarity to the group 4 LEA proteins in secondary structure. It was localized in the nucleus. The transcripts of
CarLEA4
were detected in many chickpea organs including seedling leaves, stems, roots, flowers, young pods, and young seeds.
CarLEA4
was inhibited by leaf age and showed expression changes in expression during seed development, pod development and germination. Furthermore, the expression of
CarLEA4
was strongly induced by drought, salt, heat, cold, ABA, IAA, GA
3
and MeJA. Our results suggest that
CarLEA4
encodes a protein of LEA group 4 and may be involved in various plant developmental processes and abiotic stress responses.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-011-1130-6</identifier><identifier>PMID: 21720760</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amino Acid Sequence ; Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Cell Nucleus - metabolism ; Cicer - genetics ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Genes, Plant - genetics ; Green Fluorescent Proteins - metabolism ; Histology ; Legumes ; Life Sciences ; Molecular biology ; Molecular Sequence Data ; Morphology ; Multigene Family - genetics ; Organ Specificity - genetics ; Plant biology ; Plant Growth Regulators - pharmacology ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein Structure, Tertiary ; Proteins ; Real-Time Polymerase Chain Reaction ; Sequence Alignment ; Stress, Physiological - drug effects ; Stress, Physiological - genetics</subject><ispartof>Molecular biology reports, 2012-04, Vol.39 (4), p.3565-3572</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>Springer Science+Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-3d1d41b22f0450719c186caa82dd7e46dd358d77c937057cde13877e7038bd6c3</citedby><cites>FETCH-LOGICAL-c370t-3d1d41b22f0450719c186caa82dd7e46dd358d77c937057cde13877e7038bd6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-011-1130-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-011-1130-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21720760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Hanyan</creatorcontrib><creatorcontrib>Jia, Yuying</creatorcontrib><creatorcontrib>Wang, Xiansheng</creatorcontrib><creatorcontrib>Chen, Quanjia</creatorcontrib><creatorcontrib>Shi, Shubing</creatorcontrib><creatorcontrib>Ma, Lin</creatorcontrib><creatorcontrib>Zhang, Jusong</creatorcontrib><creatorcontrib>Zhang, Hua</creatorcontrib><creatorcontrib>Ma, Hao</creatorcontrib><title>Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.)</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Late-embryogenesis abundant (LEA) proteins have been reported to be closely correlated with the acquisition of desiccation tolerance during seed development and response of plant to drought, salinity, and freezing, etc. In this study, a
LEA
gene,
CarLEA4
(GenBank accession no. GU247511), was isolated from chickpea based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol (PEG).
CarLEA4
contained two exons and one intron within genomic DNA sequence and encoded a putative polypeptide of 152 amino acids. CarLEA4 had a conserved pfam domain, and showed high similarity to the group 4 LEA proteins in secondary structure. It was localized in the nucleus. The transcripts of
CarLEA4
were detected in many chickpea organs including seedling leaves, stems, roots, flowers, young pods, and young seeds.
CarLEA4
was inhibited by leaf age and showed expression changes in expression during seed development, pod development and germination. Furthermore, the expression of
CarLEA4
was strongly induced by drought, salt, heat, cold, ABA, IAA, GA
3
and MeJA. Our results suggest that
CarLEA4
encodes a protein of LEA group 4 and may be involved in various plant developmental processes and abiotic stress responses.</description><subject>Amino Acid Sequence</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Nucleus - metabolism</subject><subject>Cicer - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Histology</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>Molecular Sequence Data</subject><subject>Morphology</subject><subject>Multigene Family - genetics</subject><subject>Organ Specificity - genetics</subject><subject>Plant biology</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Structure, Tertiary</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Sequence Alignment</subject><subject>Stress, Physiological - drug effects</subject><subject>Stress, Physiological - genetics</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kLFu2zAQhomiRey4eYAuAdEl6SD3TqREaTQMJw1goEs7EzR5SulYkkNKQ_r0oaE0AQJ0InD8_v8OH2NfEJYIoL5HRBAiA8QMUUBWfmBzLJTIZK2qj2wOAjCTVYEzdh7jHgAkquKMzXJUOagS5szeOeoG33hrBt933HSO2z8mGDtQ8H-nYd9ww7ebFW9M6w9P_J464msT0kjyJvRtinj7cCTDr9feUuAmeBp8N7Z8u_z2mX1qzCHSxcu7YL9vNr_WP7Ltz9u79WqbWaFgyIRDJ3GX5w3IAhTWFqvSGlPlzimSpXOiqJxStk54oawjFJVSpEBUO1dasWBXU-8x9I8jxUG3Plo6HExH_Rh1nZelFFAXifz6jtz3Y-jScSdIyKLKVYJwgmzoYwzU6GPwrQlPGkGf_OvJv07-9cm_LlPm8qV43LXkXhP_hCcgn4CYvrp7Cm-b_9_6DIwxjXk</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Gu, Hanyan</creator><creator>Jia, Yuying</creator><creator>Wang, Xiansheng</creator><creator>Chen, Quanjia</creator><creator>Shi, Shubing</creator><creator>Ma, Lin</creator><creator>Zhang, Jusong</creator><creator>Zhang, Hua</creator><creator>Ma, Hao</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20120401</creationdate><title>Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.)</title><author>Gu, Hanyan ; Jia, Yuying ; Wang, Xiansheng ; Chen, Quanjia ; Shi, Shubing ; Ma, Lin ; Zhang, Jusong ; Zhang, Hua ; Ma, Hao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-3d1d41b22f0450719c186caa82dd7e46dd358d77c937057cde13877e7038bd6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Nucleus - metabolism</topic><topic>Cicer - genetics</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Plant - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Histology</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Molecular biology</topic><topic>Molecular Sequence Data</topic><topic>Morphology</topic><topic>Multigene Family - genetics</topic><topic>Organ Specificity - genetics</topic><topic>Plant biology</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Protein Structure, Tertiary</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Sequence Alignment</topic><topic>Stress, Physiological - drug effects</topic><topic>Stress, Physiological - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Hanyan</creatorcontrib><creatorcontrib>Jia, Yuying</creatorcontrib><creatorcontrib>Wang, Xiansheng</creatorcontrib><creatorcontrib>Chen, Quanjia</creatorcontrib><creatorcontrib>Shi, Shubing</creatorcontrib><creatorcontrib>Ma, Lin</creatorcontrib><creatorcontrib>Zhang, Jusong</creatorcontrib><creatorcontrib>Zhang, Hua</creatorcontrib><creatorcontrib>Ma, Hao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Hanyan</au><au>Jia, Yuying</au><au>Wang, Xiansheng</au><au>Chen, Quanjia</au><au>Shi, Shubing</au><au>Ma, Lin</au><au>Zhang, Jusong</au><au>Zhang, Hua</au><au>Ma, Hao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.)</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>39</volume><issue>4</issue><spage>3565</spage><epage>3572</epage><pages>3565-3572</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Late-embryogenesis abundant (LEA) proteins have been reported to be closely correlated with the acquisition of desiccation tolerance during seed development and response of plant to drought, salinity, and freezing, etc. In this study, a
LEA
gene,
CarLEA4
(GenBank accession no. GU247511), was isolated from chickpea based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol (PEG).
CarLEA4
contained two exons and one intron within genomic DNA sequence and encoded a putative polypeptide of 152 amino acids. CarLEA4 had a conserved pfam domain, and showed high similarity to the group 4 LEA proteins in secondary structure. It was localized in the nucleus. The transcripts of
CarLEA4
were detected in many chickpea organs including seedling leaves, stems, roots, flowers, young pods, and young seeds.
CarLEA4
was inhibited by leaf age and showed expression changes in expression during seed development, pod development and germination. Furthermore, the expression of
CarLEA4
was strongly induced by drought, salt, heat, cold, ABA, IAA, GA
3
and MeJA. Our results suggest that
CarLEA4
encodes a protein of LEA group 4 and may be involved in various plant developmental processes and abiotic stress responses.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21720760</pmid><doi>10.1007/s11033-011-1130-6</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Cell Nucleus - metabolism Cicer - genetics Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant - genetics Green Fluorescent Proteins - metabolism Histology Legumes Life Sciences Molecular biology Molecular Sequence Data Morphology Multigene Family - genetics Organ Specificity - genetics Plant biology Plant Growth Regulators - pharmacology Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Protein Structure, Tertiary Proteins Real-Time Polymerase Chain Reaction Sequence Alignment Stress, Physiological - drug effects Stress, Physiological - genetics |
| title | Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.) |
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