Preparation, characterization and adsorption behavior of tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose for the selective separation of bovine serum albumin

[Display omitted] ► A novel adsorbent, tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose (TMPGZDC) was synthesized. ► Adsorbent was characterized using FTIR, XRD, SEM, TG and fluorescence analyzers. ► Adsorption characteristics of bovine serum albumin (BSA) onto...

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Veröffentlicht in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2012-05, Vol.93, p.49-58
Hauptverfasser: Anirudhan, Thayyath Sreenivasan, Rejeena, Sylaja Raveendran, Tharun, Abdul Rauf
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Rejeena, Sylaja Raveendran
Tharun, Abdul Rauf
description [Display omitted] ► A novel adsorbent, tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose (TMPGZDC) was synthesized. ► Adsorbent was characterized using FTIR, XRD, SEM, TG and fluorescence analyzers. ► Adsorption characteristics of bovine serum albumin (BSA) onto TMPGZDC were investigated under different optimized conditions. ► TMPGZDC exhibits significant adsorption potential in the recovery of BSA from aqueous solutions and protein mixtures. ► Regeneration of the spent adsorbent for several cycles was possible with 0.1M NaCl. Bovine serum albumin (BSA) is one of the major constituents of blood plasma and its recovery is a significantly useful subject for biomedical applications. In this study, a novel adsorbent, tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose (TMPGZDC) was synthesized by graft copolymerization reaction of glycidylmethacrylate onto zirconium oxide-densified cellulose (ZDC) in the presence of N,N′-methylenebisacrylamide as cross-linker followed by tannin immobilization. The adsorbent was characterized using TG, XRD, SEM/EDS, FTIR and fluorescence analyses. The efficiency of the TMPGZDC to adsorb BSA from aqueous solutions was studied at different optimized conditions. The optimum pH for maximum adsorption was found to be 4.8 with the adsorption percentage of 97.8% for an initial concentration of 100mg/L. Equilibrium was achieved within 30min. The kinetic data were found to follow pseudo-first-order model which is based on solid capacity. The well agreement of equilibrium data with Langmuir isotherm and Redlich–Peterson isotherm models confirms the monolayer coverage of BSA onto TMPGZDC surface and the maximum adsorption capacity at 30°C was found to be 93.11mg/g. Thermodynamic study revealed an exothermic adsorption process. TMPGZDC was found to be efficient in the selective adsorption of proteins. Spent adsorbent was effectively degenerated with 0.1M NaCl. The present investigation shows that TMPGZDC is a promising adsorbent for the recovery of BSA from aqueous solutions and protein mixtures.
doi_str_mv 10.1016/j.colsurfb.2011.12.010
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Bovine serum albumin (BSA) is one of the major constituents of blood plasma and its recovery is a significantly useful subject for biomedical applications. In this study, a novel adsorbent, tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose (TMPGZDC) was synthesized by graft copolymerization reaction of glycidylmethacrylate onto zirconium oxide-densified cellulose (ZDC) in the presence of N,N′-methylenebisacrylamide as cross-linker followed by tannin immobilization. The adsorbent was characterized using TG, XRD, SEM/EDS, FTIR and fluorescence analyses. The efficiency of the TMPGZDC to adsorb BSA from aqueous solutions was studied at different optimized conditions. The optimum pH for maximum adsorption was found to be 4.8 with the adsorption percentage of 97.8% for an initial concentration of 100mg/L. Equilibrium was achieved within 30min. The kinetic data were found to follow pseudo-first-order model which is based on solid capacity. The well agreement of equilibrium data with Langmuir isotherm and Redlich–Peterson isotherm models confirms the monolayer coverage of BSA onto TMPGZDC surface and the maximum adsorption capacity at 30°C was found to be 93.11mg/g. Thermodynamic study revealed an exothermic adsorption process. TMPGZDC was found to be efficient in the selective adsorption of proteins. Spent adsorbent was effectively degenerated with 0.1M NaCl. The present investigation shows that TMPGZDC is a promising adsorbent for the recovery of BSA from aqueous solutions and protein mixtures.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2011.12.010</identifier><identifier>PMID: 22230358</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adsorbents ; Adsorption ; Animals ; aqueous solutions ; blood plasma ; Bovine serum albumin ; Cattle ; Cellulose ; Cellulose - chemistry ; colloids ; Copolymerization ; Cross-Linking Reagents ; Desorption ; energy-dispersive X-ray analysis ; fluorescence ; Fourier transform infrared spectroscopy ; Graft copolymerization ; heat production ; Hydrogen-Ion Concentration ; Isotherm ; Isotherms ; Kinetics ; Polymethacrylic Acids - chemistry ; Proteins ; scanning electron microscopy ; Serum albumin ; Serum Albumin, Bovine - chemistry ; Serum Albumin, Bovine - isolation &amp; purification ; sodium chloride ; Solutions ; sorption isotherms ; Spectroscopy, Fourier Transform Infrared ; Surface chemistry ; Tannins - chemistry ; Temperature ; Thermodynamics ; Water ; X-Ray Diffraction ; zirconium ; Zirconium - chemistry</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2012-05, Vol.93, p.49-58</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. 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Bovine serum albumin (BSA) is one of the major constituents of blood plasma and its recovery is a significantly useful subject for biomedical applications. In this study, a novel adsorbent, tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose (TMPGZDC) was synthesized by graft copolymerization reaction of glycidylmethacrylate onto zirconium oxide-densified cellulose (ZDC) in the presence of N,N′-methylenebisacrylamide as cross-linker followed by tannin immobilization. The adsorbent was characterized using TG, XRD, SEM/EDS, FTIR and fluorescence analyses. The efficiency of the TMPGZDC to adsorb BSA from aqueous solutions was studied at different optimized conditions. The optimum pH for maximum adsorption was found to be 4.8 with the adsorption percentage of 97.8% for an initial concentration of 100mg/L. Equilibrium was achieved within 30min. The kinetic data were found to follow pseudo-first-order model which is based on solid capacity. The well agreement of equilibrium data with Langmuir isotherm and Redlich–Peterson isotherm models confirms the monolayer coverage of BSA onto TMPGZDC surface and the maximum adsorption capacity at 30°C was found to be 93.11mg/g. Thermodynamic study revealed an exothermic adsorption process. TMPGZDC was found to be efficient in the selective adsorption of proteins. Spent adsorbent was effectively degenerated with 0.1M NaCl. The present investigation shows that TMPGZDC is a promising adsorbent for the recovery of BSA from aqueous solutions and protein mixtures.</description><subject>Adsorbents</subject><subject>Adsorption</subject><subject>Animals</subject><subject>aqueous solutions</subject><subject>blood plasma</subject><subject>Bovine serum albumin</subject><subject>Cattle</subject><subject>Cellulose</subject><subject>Cellulose - chemistry</subject><subject>colloids</subject><subject>Copolymerization</subject><subject>Cross-Linking Reagents</subject><subject>Desorption</subject><subject>energy-dispersive X-ray analysis</subject><subject>fluorescence</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Graft copolymerization</subject><subject>heat production</subject><subject>Hydrogen-Ion Concentration</subject><subject>Isotherm</subject><subject>Isotherms</subject><subject>Kinetics</subject><subject>Polymethacrylic Acids - chemistry</subject><subject>Proteins</subject><subject>scanning electron microscopy</subject><subject>Serum albumin</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Serum Albumin, Bovine - isolation &amp; purification</subject><subject>sodium chloride</subject><subject>Solutions</subject><subject>sorption isotherms</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Surface chemistry</subject><subject>Tannins - chemistry</subject><subject>Temperature</subject><subject>Thermodynamics</subject><subject>Water</subject><subject>X-Ray Diffraction</subject><subject>zirconium</subject><subject>Zirconium - chemistry</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkttu1DAQhiMEotuFVyi5o5XI4kPiOHdFFSepEkjQa8uxx7teOfZiJyu2z8eD4XS7XAJXHo---Wc08xfFBUYrjDB7u12p4NIUTb8iCOMVJiuE0ZNigXlLq5qy9mmxQB1pq7ZlzVlxntIWIURq3D4vzgghFNGGL4pfXyPsZJSjDf5NqTY5VCNEe_-QKaXXpdQpxN3Dt4eN3NsQy2DKUXpvfTUEbY0FXe6CO1yu3UFZfXADjBup4sHJEa6qdZRmzMi9jSp4Ow1l-Gk1VBp8OhYrcG5yIUFpsvq4gTKBAzXa_RydBpzb9mFv_ZyMWUa6fhqsf1E8M9IlePn4Lou7D--_33yqbr98_Hzz7rZSddOOVcP6VgGVQGTNuekA06aDhpq6QYz1iNd1xxlBPTOacUq0YrppeW8oN6zrgS6L10fdXQw_JkijGGyaR5cewpRER1hWqvNql8XlX0nMunwKjPl_oAhxXuexWEbZEVUxpBTBiF20g4yHDInZFmIrTrYQsy0EJiLbIhdePPaY-gH0n7KTDzLw6ggYGYRcR5vE3beswLJnKEO0y8T1kYC84L2FKJKy4BVoG_OhhA72X1P8Brg422c</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Anirudhan, Thayyath Sreenivasan</creator><creator>Rejeena, Sylaja Raveendran</creator><creator>Tharun, Abdul Rauf</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>Preparation, characterization and adsorption behavior of tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose for the selective separation of bovine serum albumin</title><author>Anirudhan, Thayyath Sreenivasan ; Rejeena, Sylaja Raveendran ; Tharun, Abdul Rauf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-56b7ce3ae2a488f9e1359e53f45066b084498620b6fd6832dc6d578bf38f69be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adsorbents</topic><topic>Adsorption</topic><topic>Animals</topic><topic>aqueous solutions</topic><topic>blood plasma</topic><topic>Bovine serum albumin</topic><topic>Cattle</topic><topic>Cellulose</topic><topic>Cellulose - chemistry</topic><topic>colloids</topic><topic>Copolymerization</topic><topic>Cross-Linking Reagents</topic><topic>Desorption</topic><topic>energy-dispersive X-ray analysis</topic><topic>fluorescence</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Graft copolymerization</topic><topic>heat production</topic><topic>Hydrogen-Ion Concentration</topic><topic>Isotherm</topic><topic>Isotherms</topic><topic>Kinetics</topic><topic>Polymethacrylic Acids - chemistry</topic><topic>Proteins</topic><topic>scanning electron microscopy</topic><topic>Serum albumin</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Serum Albumin, Bovine - isolation &amp; purification</topic><topic>sodium chloride</topic><topic>Solutions</topic><topic>sorption isotherms</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Surface chemistry</topic><topic>Tannins - chemistry</topic><topic>Temperature</topic><topic>Thermodynamics</topic><topic>Water</topic><topic>X-Ray Diffraction</topic><topic>zirconium</topic><topic>Zirconium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anirudhan, Thayyath Sreenivasan</creatorcontrib><creatorcontrib>Rejeena, Sylaja Raveendran</creatorcontrib><creatorcontrib>Tharun, Abdul Rauf</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anirudhan, Thayyath Sreenivasan</au><au>Rejeena, Sylaja Raveendran</au><au>Tharun, Abdul Rauf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation, characterization and adsorption behavior of tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose for the selective separation of bovine serum albumin</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>93</volume><spage>49</spage><epage>58</epage><pages>49-58</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>[Display omitted] ► A novel adsorbent, tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose (TMPGZDC) was synthesized. ► Adsorbent was characterized using FTIR, XRD, SEM, TG and fluorescence analyzers. ► Adsorption characteristics of bovine serum albumin (BSA) onto TMPGZDC were investigated under different optimized conditions. ► TMPGZDC exhibits significant adsorption potential in the recovery of BSA from aqueous solutions and protein mixtures. ► Regeneration of the spent adsorbent for several cycles was possible with 0.1M NaCl. Bovine serum albumin (BSA) is one of the major constituents of blood plasma and its recovery is a significantly useful subject for biomedical applications. In this study, a novel adsorbent, tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose (TMPGZDC) was synthesized by graft copolymerization reaction of glycidylmethacrylate onto zirconium oxide-densified cellulose (ZDC) in the presence of N,N′-methylenebisacrylamide as cross-linker followed by tannin immobilization. The adsorbent was characterized using TG, XRD, SEM/EDS, FTIR and fluorescence analyses. The efficiency of the TMPGZDC to adsorb BSA from aqueous solutions was studied at different optimized conditions. The optimum pH for maximum adsorption was found to be 4.8 with the adsorption percentage of 97.8% for an initial concentration of 100mg/L. Equilibrium was achieved within 30min. The kinetic data were found to follow pseudo-first-order model which is based on solid capacity. The well agreement of equilibrium data with Langmuir isotherm and Redlich–Peterson isotherm models confirms the monolayer coverage of BSA onto TMPGZDC surface and the maximum adsorption capacity at 30°C was found to be 93.11mg/g. Thermodynamic study revealed an exothermic adsorption process. TMPGZDC was found to be efficient in the selective adsorption of proteins. Spent adsorbent was effectively degenerated with 0.1M NaCl. The present investigation shows that TMPGZDC is a promising adsorbent for the recovery of BSA from aqueous solutions and protein mixtures.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22230358</pmid><doi>10.1016/j.colsurfb.2011.12.010</doi><tpages>10</tpages></addata></record>
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ispartof Colloids and surfaces, B, Biointerfaces, 2012-05, Vol.93, p.49-58
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Adsorbents
Adsorption
Animals
aqueous solutions
blood plasma
Bovine serum albumin
Cattle
Cellulose
Cellulose - chemistry
colloids
Copolymerization
Cross-Linking Reagents
Desorption
energy-dispersive X-ray analysis
fluorescence
Fourier transform infrared spectroscopy
Graft copolymerization
heat production
Hydrogen-Ion Concentration
Isotherm
Isotherms
Kinetics
Polymethacrylic Acids - chemistry
Proteins
scanning electron microscopy
Serum albumin
Serum Albumin, Bovine - chemistry
Serum Albumin, Bovine - isolation & purification
sodium chloride
Solutions
sorption isotherms
Spectroscopy, Fourier Transform Infrared
Surface chemistry
Tannins - chemistry
Temperature
Thermodynamics
Water
X-Ray Diffraction
zirconium
Zirconium - chemistry
title Preparation, characterization and adsorption behavior of tannin-modified poly(glycidylmethacrylate)-grafted zirconium oxide-densified cellulose for the selective separation of bovine serum albumin
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