Determination of Bioactive Matter by Affinity Adsorption Solid Substrate–Room Temperature Phosphorimetry Based on Lectin Labeled with Self-ordered Ring of Eosin Y
A new phosphorescent labeling reagent named self-ordered ring (ESOR) of eosin Y (E) was developed. And the application of the determination of bioactive matter by affinity adsorption solid substrate–room temperature phosphorimetry (AA-SS-RTP) based on ESOR labeling lectin was studied. Results showed...
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| Veröffentlicht in: | Journal of fluorescence 2009, Vol.19 (1), p.73-83 |
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| creator | Liu, Jia-Ming Liu, Zhen-Bo Li, Wen-Qi Huang, Xiao-Mei Zeng, Li-Qing Sha, Jian Li, Fei-Ming |
| description | A new phosphorescent labeling reagent named self-ordered ring (ESOR) of eosin Y (E) was developed. And the application of the determination of bioactive matter by affinity adsorption solid substrate–room temperature phosphorimetry (AA-SS-RTP) based on ESOR labeling lectin was studied. Results showed that pink and homogeneous ESOR could be formed by E on polyamide membrane (PAM) in the presence of cetyltrimethylammonium bromide (CTAB) and ammonia water. ESOR could emit strong and stable room temperature phosphorescence (RTP) signal of E in the presence of heavy atom perturber. Specific affinity adsorption (AA) reactions could be carried out between the products of concanavalin agglutinin (Con A), triticum vulgaris lectin (WGA) labeled with ESOR and alpha-fetoprotein variant (AFP-V), alkaline phosphatase (ALP), glucose (G), respectively. Not only did the products of the affinity adsorption reactions preserve good RTP characteristic of E, but also the Δ
I
p
(Δ
I
p
=
I
p2
−
I
p1
,
I
p1
is the RTP intensity of blank reagent,
I
p2
is the RTP intensity of sample) of these products was proportional to the content of AFP-V, ALP and G, respectively. According to the facts above, a new method of AA-SS-RTP for the determination of AFP-V, ALP and G was established, based on ESOR labeling lectin. Detection limits (LD) of this method were 0.040 fg spot
−1
for AFP-V, 0.045 fg spot
−1
for ALP and 0.090 fg spot
−1
for G. And it has been successfully applied to the determination of AFP-V in human serum as well as the survey and forecast of human diseases. This method had high sensitivity, good repeatability, long RTP lifetime and little background interference with
at the long wavelength area. Meanwhile, the mechanism for the determination of trace AFP-V by AA-SS-RTP based on Con A labeled with ESOR was also discussed. |
| doi_str_mv | 10.1007/s10895-008-0383-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_926324036</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>926324036</sourcerecordid><originalsourceid>FETCH-LOGICAL-c374t-a246e017774ffc2318eef62ccdafb1da874308a3a692141e0cf45a36e30a5b813</originalsourceid><addsrcrecordid>eNp9kc9u1DAQxiMEon_gAbggn-BkGMd24hzb0gLSIlC3HDhZTjLuukri1HZAe-MdeAWejCfBy67EracZzfy-z9Z8RfGCwRsGUL-NDFQjKYCiwBWn8lFxzGTNqWga8Tj3IDkFCc1RcRLjHQA0SqinxRFTsuJSlMfF73eYMIxuMsn5iXhLzp03XXLfkXwyKe9IuyVn1rrJpdz00Yf5H7r2g-vJemljCibhn5-_rr0fyQ2OM-bBEpB82fg4b3xwI6awJecmYk-ydIX5gVxMi0Oe_HBpQ9Y4WOpDjyFPrt10u_vLpY-Z-_aseGLNEPH5oZ4WX68uby4-0NXn9x8vzla047VI1JSiQmB1XQtru5IzhWirsut6Y1vWG1ULDspwUzUlEwyhs0IaXiEHI1vF-Gnxeu87B3-_YEx6dLHDYTAT-iXqpqx4KYBXmXz1IFlVSkkhIYNsD3bBxxjQ6jmfw4StZqB3Iep9iDqHqHchapk1Lw_mSzti_19xSC0D5R6IeTXdYtB3fglTPs0Drn8BA6GrFw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66885450</pqid></control><display><type>article</type><title>Determination of Bioactive Matter by Affinity Adsorption Solid Substrate–Room Temperature Phosphorimetry Based on Lectin Labeled with Self-ordered Ring of Eosin Y</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Liu, Jia-Ming ; Liu, Zhen-Bo ; Li, Wen-Qi ; Huang, Xiao-Mei ; Zeng, Li-Qing ; Sha, Jian ; Li, Fei-Ming</creator><creatorcontrib>Liu, Jia-Ming ; Liu, Zhen-Bo ; Li, Wen-Qi ; Huang, Xiao-Mei ; Zeng, Li-Qing ; Sha, Jian ; Li, Fei-Ming</creatorcontrib><description>A new phosphorescent labeling reagent named self-ordered ring (ESOR) of eosin Y (E) was developed. And the application of the determination of bioactive matter by affinity adsorption solid substrate–room temperature phosphorimetry (AA-SS-RTP) based on ESOR labeling lectin was studied. Results showed that pink and homogeneous ESOR could be formed by E on polyamide membrane (PAM) in the presence of cetyltrimethylammonium bromide (CTAB) and ammonia water. ESOR could emit strong and stable room temperature phosphorescence (RTP) signal of E in the presence of heavy atom perturber. Specific affinity adsorption (AA) reactions could be carried out between the products of concanavalin agglutinin (Con A), triticum vulgaris lectin (WGA) labeled with ESOR and alpha-fetoprotein variant (AFP-V), alkaline phosphatase (ALP), glucose (G), respectively. Not only did the products of the affinity adsorption reactions preserve good RTP characteristic of E, but also the Δ
I
p
(Δ
I
p
=
I
p2
−
I
p1
,
I
p1
is the RTP intensity of blank reagent,
I
p2
is the RTP intensity of sample) of these products was proportional to the content of AFP-V, ALP and G, respectively. According to the facts above, a new method of AA-SS-RTP for the determination of AFP-V, ALP and G was established, based on ESOR labeling lectin. Detection limits (LD) of this method were 0.040 fg spot
−1
for AFP-V, 0.045 fg spot
−1
for ALP and 0.090 fg spot
−1
for G. And it has been successfully applied to the determination of AFP-V in human serum as well as the survey and forecast of human diseases. This method had high sensitivity, good repeatability, long RTP lifetime and little background interference with
at the long wavelength area. Meanwhile, the mechanism for the determination of trace AFP-V by AA-SS-RTP based on Con A labeled with ESOR was also discussed.</description><identifier>ISSN: 1053-0509</identifier><identifier>EISSN: 1573-4994</identifier><identifier>DOI: 10.1007/s10895-008-0383-5</identifier><identifier>PMID: 18563542</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Adsorption ; Affinity ; Alkaline Phosphatase - analysis ; alpha-Fetoproteins - analysis ; Analytical Chemistry ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedicine ; Biophysics ; Biotechnology ; Concanavalin A - chemistry ; Diseases ; Eosine Yellowish-(YS) - chemistry ; Glucose - analysis ; Human ; Humidity ; Lectins ; Luminescent Measurements - instrumentation ; Luminescent Measurements - methods ; Marking ; Molecular Structure ; Original Paper ; Oxygen - chemistry ; Polyamide resins ; Preserves ; Sensitivity and Specificity ; Spectrophotometry - instrumentation ; Spectrophotometry - methods ; Staining and Labeling ; Temperature ; Time Factors ; Wheat Germ Agglutinins - chemistry</subject><ispartof>Journal of fluorescence, 2009, Vol.19 (1), p.73-83</ispartof><rights>Springer Science+Business Media, LLC 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-a246e017774ffc2318eef62ccdafb1da874308a3a692141e0cf45a36e30a5b813</citedby><cites>FETCH-LOGICAL-c374t-a246e017774ffc2318eef62ccdafb1da874308a3a692141e0cf45a36e30a5b813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10895-008-0383-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10895-008-0383-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18563542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Jia-Ming</creatorcontrib><creatorcontrib>Liu, Zhen-Bo</creatorcontrib><creatorcontrib>Li, Wen-Qi</creatorcontrib><creatorcontrib>Huang, Xiao-Mei</creatorcontrib><creatorcontrib>Zeng, Li-Qing</creatorcontrib><creatorcontrib>Sha, Jian</creatorcontrib><creatorcontrib>Li, Fei-Ming</creatorcontrib><title>Determination of Bioactive Matter by Affinity Adsorption Solid Substrate–Room Temperature Phosphorimetry Based on Lectin Labeled with Self-ordered Ring of Eosin Y</title><title>Journal of fluorescence</title><addtitle>J Fluoresc</addtitle><addtitle>J Fluoresc</addtitle><description>A new phosphorescent labeling reagent named self-ordered ring (ESOR) of eosin Y (E) was developed. And the application of the determination of bioactive matter by affinity adsorption solid substrate–room temperature phosphorimetry (AA-SS-RTP) based on ESOR labeling lectin was studied. Results showed that pink and homogeneous ESOR could be formed by E on polyamide membrane (PAM) in the presence of cetyltrimethylammonium bromide (CTAB) and ammonia water. ESOR could emit strong and stable room temperature phosphorescence (RTP) signal of E in the presence of heavy atom perturber. Specific affinity adsorption (AA) reactions could be carried out between the products of concanavalin agglutinin (Con A), triticum vulgaris lectin (WGA) labeled with ESOR and alpha-fetoprotein variant (AFP-V), alkaline phosphatase (ALP), glucose (G), respectively. Not only did the products of the affinity adsorption reactions preserve good RTP characteristic of E, but also the Δ
I
p
(Δ
I
p
=
I
p2
−
I
p1
,
I
p1
is the RTP intensity of blank reagent,
I
p2
is the RTP intensity of sample) of these products was proportional to the content of AFP-V, ALP and G, respectively. According to the facts above, a new method of AA-SS-RTP for the determination of AFP-V, ALP and G was established, based on ESOR labeling lectin. Detection limits (LD) of this method were 0.040 fg spot
−1
for AFP-V, 0.045 fg spot
−1
for ALP and 0.090 fg spot
−1
for G. And it has been successfully applied to the determination of AFP-V in human serum as well as the survey and forecast of human diseases. This method had high sensitivity, good repeatability, long RTP lifetime and little background interference with
at the long wavelength area. Meanwhile, the mechanism for the determination of trace AFP-V by AA-SS-RTP based on Con A labeled with ESOR was also discussed.</description><subject>Adsorption</subject><subject>Affinity</subject><subject>Alkaline Phosphatase - analysis</subject><subject>alpha-Fetoproteins - analysis</subject><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Concanavalin A - chemistry</subject><subject>Diseases</subject><subject>Eosine Yellowish-(YS) - chemistry</subject><subject>Glucose - analysis</subject><subject>Human</subject><subject>Humidity</subject><subject>Lectins</subject><subject>Luminescent Measurements - instrumentation</subject><subject>Luminescent Measurements - methods</subject><subject>Marking</subject><subject>Molecular Structure</subject><subject>Original Paper</subject><subject>Oxygen - chemistry</subject><subject>Polyamide resins</subject><subject>Preserves</subject><subject>Sensitivity and Specificity</subject><subject>Spectrophotometry - instrumentation</subject><subject>Spectrophotometry - methods</subject><subject>Staining and Labeling</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Wheat Germ Agglutinins - chemistry</subject><issn>1053-0509</issn><issn>1573-4994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQxiMEon_gAbggn-BkGMd24hzb0gLSIlC3HDhZTjLuukri1HZAe-MdeAWejCfBy67EracZzfy-z9Z8RfGCwRsGUL-NDFQjKYCiwBWn8lFxzGTNqWga8Tj3IDkFCc1RcRLjHQA0SqinxRFTsuJSlMfF73eYMIxuMsn5iXhLzp03XXLfkXwyKe9IuyVn1rrJpdz00Yf5H7r2g-vJemljCibhn5-_rr0fyQ2OM-bBEpB82fg4b3xwI6awJecmYk-ydIX5gVxMi0Oe_HBpQ9Y4WOpDjyFPrt10u_vLpY-Z-_aseGLNEPH5oZ4WX68uby4-0NXn9x8vzla047VI1JSiQmB1XQtru5IzhWirsut6Y1vWG1ULDspwUzUlEwyhs0IaXiEHI1vF-Gnxeu87B3-_YEx6dLHDYTAT-iXqpqx4KYBXmXz1IFlVSkkhIYNsD3bBxxjQ6jmfw4StZqB3Iep9iDqHqHchapk1Lw_mSzti_19xSC0D5R6IeTXdYtB3fglTPs0Drn8BA6GrFw</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Liu, Jia-Ming</creator><creator>Liu, Zhen-Bo</creator><creator>Li, Wen-Qi</creator><creator>Huang, Xiao-Mei</creator><creator>Zeng, Li-Qing</creator><creator>Sha, Jian</creator><creator>Li, Fei-Ming</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>2009</creationdate><title>Determination of Bioactive Matter by Affinity Adsorption Solid Substrate–Room Temperature Phosphorimetry Based on Lectin Labeled with Self-ordered Ring of Eosin Y</title><author>Liu, Jia-Ming ; Liu, Zhen-Bo ; Li, Wen-Qi ; Huang, Xiao-Mei ; Zeng, Li-Qing ; Sha, Jian ; Li, Fei-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-a246e017774ffc2318eef62ccdafb1da874308a3a692141e0cf45a36e30a5b813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adsorption</topic><topic>Affinity</topic><topic>Alkaline Phosphatase - analysis</topic><topic>alpha-Fetoproteins - analysis</topic><topic>Analytical Chemistry</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Concanavalin A - chemistry</topic><topic>Diseases</topic><topic>Eosine Yellowish-(YS) - chemistry</topic><topic>Glucose - analysis</topic><topic>Human</topic><topic>Humidity</topic><topic>Lectins</topic><topic>Luminescent Measurements - instrumentation</topic><topic>Luminescent Measurements - methods</topic><topic>Marking</topic><topic>Molecular Structure</topic><topic>Original Paper</topic><topic>Oxygen - chemistry</topic><topic>Polyamide resins</topic><topic>Preserves</topic><topic>Sensitivity and Specificity</topic><topic>Spectrophotometry - instrumentation</topic><topic>Spectrophotometry - methods</topic><topic>Staining and Labeling</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Wheat Germ Agglutinins - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Jia-Ming</creatorcontrib><creatorcontrib>Liu, Zhen-Bo</creatorcontrib><creatorcontrib>Li, Wen-Qi</creatorcontrib><creatorcontrib>Huang, Xiao-Mei</creatorcontrib><creatorcontrib>Zeng, Li-Qing</creatorcontrib><creatorcontrib>Sha, Jian</creatorcontrib><creatorcontrib>Li, Fei-Ming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of fluorescence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Jia-Ming</au><au>Liu, Zhen-Bo</au><au>Li, Wen-Qi</au><au>Huang, Xiao-Mei</au><au>Zeng, Li-Qing</au><au>Sha, Jian</au><au>Li, Fei-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Bioactive Matter by Affinity Adsorption Solid Substrate–Room Temperature Phosphorimetry Based on Lectin Labeled with Self-ordered Ring of Eosin Y</atitle><jtitle>Journal of fluorescence</jtitle><stitle>J Fluoresc</stitle><addtitle>J Fluoresc</addtitle><date>2009</date><risdate>2009</risdate><volume>19</volume><issue>1</issue><spage>73</spage><epage>83</epage><pages>73-83</pages><issn>1053-0509</issn><eissn>1573-4994</eissn><abstract>A new phosphorescent labeling reagent named self-ordered ring (ESOR) of eosin Y (E) was developed. And the application of the determination of bioactive matter by affinity adsorption solid substrate–room temperature phosphorimetry (AA-SS-RTP) based on ESOR labeling lectin was studied. Results showed that pink and homogeneous ESOR could be formed by E on polyamide membrane (PAM) in the presence of cetyltrimethylammonium bromide (CTAB) and ammonia water. ESOR could emit strong and stable room temperature phosphorescence (RTP) signal of E in the presence of heavy atom perturber. Specific affinity adsorption (AA) reactions could be carried out between the products of concanavalin agglutinin (Con A), triticum vulgaris lectin (WGA) labeled with ESOR and alpha-fetoprotein variant (AFP-V), alkaline phosphatase (ALP), glucose (G), respectively. Not only did the products of the affinity adsorption reactions preserve good RTP characteristic of E, but also the Δ
I
p
(Δ
I
p
=
I
p2
−
I
p1
,
I
p1
is the RTP intensity of blank reagent,
I
p2
is the RTP intensity of sample) of these products was proportional to the content of AFP-V, ALP and G, respectively. According to the facts above, a new method of AA-SS-RTP for the determination of AFP-V, ALP and G was established, based on ESOR labeling lectin. Detection limits (LD) of this method were 0.040 fg spot
−1
for AFP-V, 0.045 fg spot
−1
for ALP and 0.090 fg spot
−1
for G. And it has been successfully applied to the determination of AFP-V in human serum as well as the survey and forecast of human diseases. This method had high sensitivity, good repeatability, long RTP lifetime and little background interference with
at the long wavelength area. Meanwhile, the mechanism for the determination of trace AFP-V by AA-SS-RTP based on Con A labeled with ESOR was also discussed.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>18563542</pmid><doi>10.1007/s10895-008-0383-5</doi><tpages>11</tpages></addata></record> |
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| subjects | Adsorption Affinity Alkaline Phosphatase - analysis alpha-Fetoproteins - analysis Analytical Chemistry Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Concanavalin A - chemistry Diseases Eosine Yellowish-(YS) - chemistry Glucose - analysis Human Humidity Lectins Luminescent Measurements - instrumentation Luminescent Measurements - methods Marking Molecular Structure Original Paper Oxygen - chemistry Polyamide resins Preserves Sensitivity and Specificity Spectrophotometry - instrumentation Spectrophotometry - methods Staining and Labeling Temperature Time Factors Wheat Germ Agglutinins - chemistry |
| title | Determination of Bioactive Matter by Affinity Adsorption Solid Substrate–Room Temperature Phosphorimetry Based on Lectin Labeled with Self-ordered Ring of Eosin Y |
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