Mechanisms of Ceftazidime and Ciprofloxacin Transport through Porins in Multidrug-Resistance Developed by Extended-Spectrum Beta-Lactamase E.coli Strains
Resistance towards antibiotics stands out today as a major issue in the clinical act of treatment of bacterial-generated infections. This process was characterized in proteoliposomes reconstituted from an E.coli strain isolated from invasive infections (blood culture) occurred in patients with a car...
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| Veröffentlicht in: | Journal of fluorescence 2011-07, Vol.21 (4), p.1421-1429 |
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| creator | Radu, Beatrice Mihaela Bacalum, Mihaela Marin, Adela Chifiriuc, Carmen-Mariana Lazar, Veronica Radu, Mihai |
| description | Resistance towards antibiotics stands out today as a major issue in the clinical act of treatment of bacterial-generated infections. This process was characterized in proteoliposomes reconstituted from an
E.coli
strain isolated from invasive infections (blood culture) occurred in patients with a cardio-vascular device admitted for surgery. Fluorescence spectroscopy and patch-clamp technique have been used. Two types of antibiotics have been targeted: ceftazidime and ciprofloxacin. Antibiotics addition in proteoliposomes suspension undergoes a quenching in tryptophan residues from outer membrane porins structure, probably due to the formation of a transient non-fluorescent porin-antibiotic complex. Patch-clamp recordings revealed strong ion current blockages for both antibiotics, reflecting antibiotic–channel interactions but with varying strength of interaction. The present study puts forward the mechanism of multidrug-resistance in extended-spectrum beta-lactamase
E.coli
strains, as being caused by alterations of the antibiotics transport across the porins of the outer bacterial membrane. |
| doi_str_mv | 10.1007/s10895-010-0826-7 |
| format | Article |
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E.coli
strain isolated from invasive infections (blood culture) occurred in patients with a cardio-vascular device admitted for surgery. Fluorescence spectroscopy and patch-clamp technique have been used. Two types of antibiotics have been targeted: ceftazidime and ciprofloxacin. Antibiotics addition in proteoliposomes suspension undergoes a quenching in tryptophan residues from outer membrane porins structure, probably due to the formation of a transient non-fluorescent porin-antibiotic complex. Patch-clamp recordings revealed strong ion current blockages for both antibiotics, reflecting antibiotic–channel interactions but with varying strength of interaction. The present study puts forward the mechanism of multidrug-resistance in extended-spectrum beta-lactamase
E.coli
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E.coli
strain isolated from invasive infections (blood culture) occurred in patients with a cardio-vascular device admitted for surgery. Fluorescence spectroscopy and patch-clamp technique have been used. Two types of antibiotics have been targeted: ceftazidime and ciprofloxacin. Antibiotics addition in proteoliposomes suspension undergoes a quenching in tryptophan residues from outer membrane porins structure, probably due to the formation of a transient non-fluorescent porin-antibiotic complex. Patch-clamp recordings revealed strong ion current blockages for both antibiotics, reflecting antibiotic–channel interactions but with varying strength of interaction. The present study puts forward the mechanism of multidrug-resistance in extended-spectrum beta-lactamase
E.coli
strains, as being caused by alterations of the antibiotics transport across the porins of the outer bacterial membrane.</description><subject>Analytical Chemistry</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>beta-Lactamase Inhibitors</subject><subject>beta-Lactamases - metabolism</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biological Transport - drug effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Ceftazidime - chemistry</subject><subject>Ceftazidime - metabolism</subject><subject>Ceftazidime - pharmacology</subject><subject>Ciprofloxacin - chemistry</subject><subject>Ciprofloxacin - metabolism</subject><subject>Ciprofloxacin - pharmacology</subject><subject>Devices</subject><subject>Drug Resistance, Multiple, Bacterial - drug effects</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - enzymology</subject><subject>Fluorescence</subject><subject>Fluorescence Resonance Energy Transfer</subject><subject>Humans</subject><subject>Membranes</subject><subject>Microbial Sensitivity Tests</subject><subject>Original Paper</subject><subject>Porins - chemistry</subject><subject>Porins - metabolism</subject><subject>Strain</subject><subject>Structure-Activity Relationship</subject><subject>Transport</subject><subject>Tryptophan</subject><issn>1053-0509</issn><issn>1573-4994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhiMEoqXwA7gg3-Di4o_ETo6wLB_SViBaztYknuy6SuxgO6jln_BvcbWFY08z0jzzSjNPVb3k7Jwzpt8mztquoYwzylqhqH5UnfJGS1p3Xf249KyRlDWsO6mepXTNGOvaun1anQguZK0UP63-XOBwAO_SnEgYyQbHDL-ddTMS8JZs3BLDOIUbGJwnVxF8WkLMJB9iWPcH8i1E5xMps4t1ys7GdU-_Y3Ipgx-QfMBfOIUFLelvyfYmo7do6eWCQ47rTN5jBrqDIcMMCcn2fAiTI5c5Qgl9Xj0ZYUr44r6eVT8-bq82n-nu66cvm3c7OtSszZT3PRs5lD-oRo8SGwmi5r2yVmBt607WYC12I-q21xL4wFsLbas0aMVAgDyrXh9zy6U_V0zZzC4NOE3gMazJdEJJwRvFCvnmQZIrzUXNZCcLyo_oEENKEUezRDdDvDWcmTt35ujOFHfmzp3RZefVffzaz2j_b_yTVQBxBFIZ-T1Gcx3W6MtzHkj9CzSspyg</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Radu, Beatrice Mihaela</creator><creator>Bacalum, Mihaela</creator><creator>Marin, Adela</creator><creator>Chifiriuc, Carmen-Mariana</creator><creator>Lazar, Veronica</creator><creator>Radu, Mihai</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20110701</creationdate><title>Mechanisms of Ceftazidime and Ciprofloxacin Transport through Porins in Multidrug-Resistance Developed by Extended-Spectrum Beta-Lactamase E.coli Strains</title><author>Radu, Beatrice Mihaela ; Bacalum, Mihaela ; Marin, Adela ; Chifiriuc, Carmen-Mariana ; Lazar, Veronica ; Radu, Mihai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-1bb0f1a895657f3e53a241b6dd2e4d4934adde9fe78b73a1c18da8867a760a2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical Chemistry</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>beta-Lactamase Inhibitors</topic><topic>beta-Lactamases - metabolism</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biological Transport - drug effects</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Ceftazidime - chemistry</topic><topic>Ceftazidime - metabolism</topic><topic>Ceftazidime - pharmacology</topic><topic>Ciprofloxacin - chemistry</topic><topic>Ciprofloxacin - metabolism</topic><topic>Ciprofloxacin - pharmacology</topic><topic>Devices</topic><topic>Drug Resistance, Multiple, Bacterial - drug effects</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - enzymology</topic><topic>Fluorescence</topic><topic>Fluorescence Resonance Energy Transfer</topic><topic>Humans</topic><topic>Membranes</topic><topic>Microbial Sensitivity Tests</topic><topic>Original Paper</topic><topic>Porins - chemistry</topic><topic>Porins - metabolism</topic><topic>Strain</topic><topic>Structure-Activity Relationship</topic><topic>Transport</topic><topic>Tryptophan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radu, Beatrice Mihaela</creatorcontrib><creatorcontrib>Bacalum, Mihaela</creatorcontrib><creatorcontrib>Marin, Adela</creatorcontrib><creatorcontrib>Chifiriuc, Carmen-Mariana</creatorcontrib><creatorcontrib>Lazar, Veronica</creatorcontrib><creatorcontrib>Radu, Mihai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of fluorescence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radu, Beatrice Mihaela</au><au>Bacalum, Mihaela</au><au>Marin, Adela</au><au>Chifiriuc, Carmen-Mariana</au><au>Lazar, Veronica</au><au>Radu, Mihai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanisms of Ceftazidime and Ciprofloxacin Transport through Porins in Multidrug-Resistance Developed by Extended-Spectrum Beta-Lactamase E.coli Strains</atitle><jtitle>Journal of fluorescence</jtitle><stitle>J Fluoresc</stitle><addtitle>J Fluoresc</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>21</volume><issue>4</issue><spage>1421</spage><epage>1429</epage><pages>1421-1429</pages><issn>1053-0509</issn><eissn>1573-4994</eissn><abstract>Resistance towards antibiotics stands out today as a major issue in the clinical act of treatment of bacterial-generated infections. This process was characterized in proteoliposomes reconstituted from an
E.coli
strain isolated from invasive infections (blood culture) occurred in patients with a cardio-vascular device admitted for surgery. Fluorescence spectroscopy and patch-clamp technique have been used. Two types of antibiotics have been targeted: ceftazidime and ciprofloxacin. Antibiotics addition in proteoliposomes suspension undergoes a quenching in tryptophan residues from outer membrane porins structure, probably due to the formation of a transient non-fluorescent porin-antibiotic complex. Patch-clamp recordings revealed strong ion current blockages for both antibiotics, reflecting antibiotic–channel interactions but with varying strength of interaction. The present study puts forward the mechanism of multidrug-resistance in extended-spectrum beta-lactamase
E.coli
strains, as being caused by alterations of the antibiotics transport across the porins of the outer bacterial membrane.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>21234661</pmid><doi>10.1007/s10895-010-0826-7</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of fluorescence, 2011-07, Vol.21 (4), p.1421-1429 |
| issn | 1053-0509 1573-4994 |
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| subjects | Analytical Chemistry Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology Antibiotics Bacteria beta-Lactamase Inhibitors beta-Lactamases - metabolism Biochemistry Biological and Medical Physics Biological Transport - drug effects Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Ceftazidime - chemistry Ceftazidime - metabolism Ceftazidime - pharmacology Ciprofloxacin - chemistry Ciprofloxacin - metabolism Ciprofloxacin - pharmacology Devices Drug Resistance, Multiple, Bacterial - drug effects Escherichia coli - drug effects Escherichia coli - enzymology Fluorescence Fluorescence Resonance Energy Transfer Humans Membranes Microbial Sensitivity Tests Original Paper Porins - chemistry Porins - metabolism Strain Structure-Activity Relationship Transport Tryptophan |
| title | Mechanisms of Ceftazidime and Ciprofloxacin Transport through Porins in Multidrug-Resistance Developed by Extended-Spectrum Beta-Lactamase E.coli Strains |
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