Metabolic profiling of urine in young obese men using ultra performance liquid chromatography and Q-TOF mass spectrometry (UPLC/Q-TOF MS)
Obesity is currently epidemic in many countries worldwide. In the young adult, obesity often accompanies hyperlipemia, which is strongly related to the occurrence and development of obesity-related chronic diseases such as diabetes mellitus, hypertension and cardiovascular disease. This study invest...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-10, Vol.879 (27), p.2871-2876 |
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| creator | Wang, Cheng Feng, Rennan Sun, Dianjun Li, Ying Bi, Xinxin Sun, Changhao |
| description | Obesity is currently epidemic in many countries worldwide. In the young adult, obesity often accompanies hyperlipemia, which is strongly related to the occurrence and development of obesity-related chronic diseases such as diabetes mellitus, hypertension and cardiovascular disease. This study investigated the differences in metabolomic profiling between obese (with hyperlipemia,
n
=
30) and normal-weight (
n
=
30) young men. Anthropometric parameters and conventional metabolites were measured. There were no significant differences between obese and normal-weight young men in age, height and fasting plasma glucose level. Obese young men showed increased weight, body mass index, fat mass, systolic blood pressure, and triglyeride, total cholesterol and insulin levels, and lower levels of testosterone. The endogenous metabolite profile of urine was investigated by UPLC/Q-TOF MS (ultra performance liquid chromatography and Q-TOF mass spectrometry) with electrospray ionization (ESI). Partial least squares (PLS) enabled clusters to be visualized. Eight urine principal metabolites contributing to the clusters were identified; these included increased
l-prolyl-
l-proline, leucyl-phenylalanine, and decanoylcarnitine in positive ESI mode (
m/
z 213.1267, 279.1715 and 316.2459, respectively) and N-acetylornithine, 17-hydroxypregnenolone sulfate, 11β-hydroxyprogesterone, 5a-dihydrotestosterone sulfate and glucosylgalactosyl hydroxylysine in negative ESI mode (
m/
z 173.0931, 411.1883, 331.185, 369.1751 and 485.1875, respectively). These metabolite changes in obese men suggested early changes of metabolism in young-male obesity with hyperlipemia. The study may further aid the clinical prevention and treatment of obesity and related chronic disease. |
| doi_str_mv | 10.1016/j.jchromb.2011.08.014 |
| format | Article |
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n
=
30) and normal-weight (
n
=
30) young men. Anthropometric parameters and conventional metabolites were measured. There were no significant differences between obese and normal-weight young men in age, height and fasting plasma glucose level. Obese young men showed increased weight, body mass index, fat mass, systolic blood pressure, and triglyeride, total cholesterol and insulin levels, and lower levels of testosterone. The endogenous metabolite profile of urine was investigated by UPLC/Q-TOF MS (ultra performance liquid chromatography and Q-TOF mass spectrometry) with electrospray ionization (ESI). Partial least squares (PLS) enabled clusters to be visualized. Eight urine principal metabolites contributing to the clusters were identified; these included increased
l-prolyl-
l-proline, leucyl-phenylalanine, and decanoylcarnitine in positive ESI mode (
m/
z 213.1267, 279.1715 and 316.2459, respectively) and N-acetylornithine, 17-hydroxypregnenolone sulfate, 11β-hydroxyprogesterone, 5a-dihydrotestosterone sulfate and glucosylgalactosyl hydroxylysine in negative ESI mode (
m/
z 173.0931, 411.1883, 331.185, 369.1751 and 485.1875, respectively). These metabolite changes in obese men suggested early changes of metabolism in young-male obesity with hyperlipemia. The study may further aid the clinical prevention and treatment of obesity and related chronic disease.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2011.08.014</identifier><identifier>PMID: 21890434</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; blood glucose ; body mass index ; Case-Control Studies ; cholesterol ; Chromatography, High Pressure Liquid - methods ; chronic diseases ; Cluster Analysis ; diabetes mellitus ; fasting ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Humans ; hydroxylysine ; Hyperlipemia ; hyperlipidemia ; Hyperlipidemias - metabolism ; Hyperlipidemias - urine ; hypertension ; insulin ; ionization ; least squares ; Least-Squares Analysis ; Male ; mass spectrometry ; Medical sciences ; men ; Metabolic profiles ; metabolism ; metabolites ; Metabolome ; metabolomics ; Multivariate Analysis ; obesity ; Obesity - metabolism ; Obesity - urine ; Pharmacology. Drug treatments ; Spectrometry, Mass, Electrospray Ionization - methods ; sulfates ; systolic blood pressure ; testosterone ; ultra-performance liquid chromatography ; UPLC/Q-TOF MS ; urine ; young adults ; Young obese men</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-10, Vol.879 (27), p.2871-2876</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><rights>Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-8f096497058a584fba0b948ce527b2ddebe16c0fa58eb515fb0acfc990c79b933</citedby><cites>FETCH-LOGICAL-c450t-8f096497058a584fba0b948ce527b2ddebe16c0fa58eb515fb0acfc990c79b933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S157002321100540X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24559063$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21890434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Cheng</creatorcontrib><creatorcontrib>Feng, Rennan</creatorcontrib><creatorcontrib>Sun, Dianjun</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Bi, Xinxin</creatorcontrib><creatorcontrib>Sun, Changhao</creatorcontrib><title>Metabolic profiling of urine in young obese men using ultra performance liquid chromatography and Q-TOF mass spectrometry (UPLC/Q-TOF MS)</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>Obesity is currently epidemic in many countries worldwide. In the young adult, obesity often accompanies hyperlipemia, which is strongly related to the occurrence and development of obesity-related chronic diseases such as diabetes mellitus, hypertension and cardiovascular disease. This study investigated the differences in metabolomic profiling between obese (with hyperlipemia,
n
=
30) and normal-weight (
n
=
30) young men. Anthropometric parameters and conventional metabolites were measured. There were no significant differences between obese and normal-weight young men in age, height and fasting plasma glucose level. Obese young men showed increased weight, body mass index, fat mass, systolic blood pressure, and triglyeride, total cholesterol and insulin levels, and lower levels of testosterone. The endogenous metabolite profile of urine was investigated by UPLC/Q-TOF MS (ultra performance liquid chromatography and Q-TOF mass spectrometry) with electrospray ionization (ESI). Partial least squares (PLS) enabled clusters to be visualized. Eight urine principal metabolites contributing to the clusters were identified; these included increased
l-prolyl-
l-proline, leucyl-phenylalanine, and decanoylcarnitine in positive ESI mode (
m/
z 213.1267, 279.1715 and 316.2459, respectively) and N-acetylornithine, 17-hydroxypregnenolone sulfate, 11β-hydroxyprogesterone, 5a-dihydrotestosterone sulfate and glucosylgalactosyl hydroxylysine in negative ESI mode (
m/
z 173.0931, 411.1883, 331.185, 369.1751 and 485.1875, respectively). These metabolite changes in obese men suggested early changes of metabolism in young-male obesity with hyperlipemia. The study may further aid the clinical prevention and treatment of obesity and related chronic disease.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>blood glucose</subject><subject>body mass index</subject><subject>Case-Control Studies</subject><subject>cholesterol</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>chronic diseases</subject><subject>Cluster Analysis</subject><subject>diabetes mellitus</subject><subject>fasting</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>hydroxylysine</subject><subject>Hyperlipemia</subject><subject>hyperlipidemia</subject><subject>Hyperlipidemias - metabolism</subject><subject>Hyperlipidemias - urine</subject><subject>hypertension</subject><subject>insulin</subject><subject>ionization</subject><subject>least squares</subject><subject>Least-Squares Analysis</subject><subject>Male</subject><subject>mass spectrometry</subject><subject>Medical sciences</subject><subject>men</subject><subject>Metabolic profiles</subject><subject>metabolism</subject><subject>metabolites</subject><subject>Metabolome</subject><subject>metabolomics</subject><subject>Multivariate Analysis</subject><subject>obesity</subject><subject>Obesity - metabolism</subject><subject>Obesity - urine</subject><subject>Pharmacology. Drug treatments</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>sulfates</subject><subject>systolic blood pressure</subject><subject>testosterone</subject><subject>ultra-performance liquid chromatography</subject><subject>UPLC/Q-TOF MS</subject><subject>urine</subject><subject>young adults</subject><subject>Young obese men</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEon_gEQBfUOGQ7TiOk_iE0IoC0lYFtStxs2xnvPUqibd2grSPwFvjsAscKx9seX4z8818WfaKwoICrS63i625D77XiwIoXUCzAFo-yU5pU7Oc1dWPp-nNa8ihYMVJdhbjFoDWULPn2UlBGwElK0-zX9c4Ku07Z8gueOs6N2yIt2QKbkDiBrL30_yjMSLpcSBTnImpG4MiOwzWh14NBknnHibXkj-a1Og3Qe3u90QNLfme391ckV7FSOIOzZgAHMOevFt_Wy0vD9Hr2_cvsmdWdRFfHu_zbH316W75JV_dfP66_LjKTclhzBsLoipFDbxRvCmtVqBF2RjkRa2LtkWNtDJgUxA1p9xqUMYaIcDUQgvGzrOLQ90078OEcZS9iwa7Tg3opyhFUTFgdTqPkY1gdQGczTX5gTTBxxjQyl1wvQp7SUHOdsmtPNolZ7skNDLZlfJeHztMusf2X9ZffxLw9gioaFRnQ9q1i_-5knMB1SzgzYGzyku1CYlZ36ZOFQBUnJfzMB8OBKbd_nQYZDQOk3OtC8kU2Xr3iNjfA2K_4Q</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Wang, Cheng</creator><creator>Feng, Rennan</creator><creator>Sun, Dianjun</creator><creator>Li, Ying</creator><creator>Bi, Xinxin</creator><creator>Sun, Changhao</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TS</scope></search><sort><creationdate>20111001</creationdate><title>Metabolic profiling of urine in young obese men using ultra performance liquid chromatography and Q-TOF mass spectrometry (UPLC/Q-TOF MS)</title><author>Wang, Cheng ; Feng, Rennan ; Sun, Dianjun ; Li, Ying ; Bi, Xinxin ; Sun, Changhao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-8f096497058a584fba0b948ce527b2ddebe16c0fa58eb515fb0acfc990c79b933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>blood glucose</topic><topic>body mass index</topic><topic>Case-Control Studies</topic><topic>cholesterol</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>chronic diseases</topic><topic>Cluster Analysis</topic><topic>diabetes mellitus</topic><topic>fasting</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>hydroxylysine</topic><topic>Hyperlipemia</topic><topic>hyperlipidemia</topic><topic>Hyperlipidemias - metabolism</topic><topic>Hyperlipidemias - urine</topic><topic>hypertension</topic><topic>insulin</topic><topic>ionization</topic><topic>least squares</topic><topic>Least-Squares Analysis</topic><topic>Male</topic><topic>mass spectrometry</topic><topic>Medical sciences</topic><topic>men</topic><topic>Metabolic profiles</topic><topic>metabolism</topic><topic>metabolites</topic><topic>Metabolome</topic><topic>metabolomics</topic><topic>Multivariate Analysis</topic><topic>obesity</topic><topic>Obesity - metabolism</topic><topic>Obesity - urine</topic><topic>Pharmacology. Drug treatments</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>sulfates</topic><topic>systolic blood pressure</topic><topic>testosterone</topic><topic>ultra-performance liquid chromatography</topic><topic>UPLC/Q-TOF MS</topic><topic>urine</topic><topic>young adults</topic><topic>Young obese men</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Cheng</creatorcontrib><creatorcontrib>Feng, Rennan</creatorcontrib><creatorcontrib>Sun, Dianjun</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Bi, Xinxin</creatorcontrib><creatorcontrib>Sun, Changhao</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Physical Education Index</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Cheng</au><au>Feng, Rennan</au><au>Sun, Dianjun</au><au>Li, Ying</au><au>Bi, Xinxin</au><au>Sun, Changhao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic profiling of urine in young obese men using ultra performance liquid chromatography and Q-TOF mass spectrometry (UPLC/Q-TOF MS)</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>879</volume><issue>27</issue><spage>2871</spage><epage>2876</epage><pages>2871-2876</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>Obesity is currently epidemic in many countries worldwide. In the young adult, obesity often accompanies hyperlipemia, which is strongly related to the occurrence and development of obesity-related chronic diseases such as diabetes mellitus, hypertension and cardiovascular disease. This study investigated the differences in metabolomic profiling between obese (with hyperlipemia,
n
=
30) and normal-weight (
n
=
30) young men. Anthropometric parameters and conventional metabolites were measured. There were no significant differences between obese and normal-weight young men in age, height and fasting plasma glucose level. Obese young men showed increased weight, body mass index, fat mass, systolic blood pressure, and triglyeride, total cholesterol and insulin levels, and lower levels of testosterone. The endogenous metabolite profile of urine was investigated by UPLC/Q-TOF MS (ultra performance liquid chromatography and Q-TOF mass spectrometry) with electrospray ionization (ESI). Partial least squares (PLS) enabled clusters to be visualized. Eight urine principal metabolites contributing to the clusters were identified; these included increased
l-prolyl-
l-proline, leucyl-phenylalanine, and decanoylcarnitine in positive ESI mode (
m/
z 213.1267, 279.1715 and 316.2459, respectively) and N-acetylornithine, 17-hydroxypregnenolone sulfate, 11β-hydroxyprogesterone, 5a-dihydrotestosterone sulfate and glucosylgalactosyl hydroxylysine in negative ESI mode (
m/
z 173.0931, 411.1883, 331.185, 369.1751 and 485.1875, respectively). These metabolite changes in obese men suggested early changes of metabolism in young-male obesity with hyperlipemia. The study may further aid the clinical prevention and treatment of obesity and related chronic disease.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21890434</pmid><doi>10.1016/j.jchromb.2011.08.014</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-10, Vol.879 (27), p.2871-2876 |
| issn | 1570-0232 1873-376X |
| language | eng |
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| subjects | Adolescent Adult Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences blood glucose body mass index Case-Control Studies cholesterol Chromatography, High Pressure Liquid - methods chronic diseases Cluster Analysis diabetes mellitus fasting Fundamental and applied biological sciences. Psychology General pharmacology Humans hydroxylysine Hyperlipemia hyperlipidemia Hyperlipidemias - metabolism Hyperlipidemias - urine hypertension insulin ionization least squares Least-Squares Analysis Male mass spectrometry Medical sciences men Metabolic profiles metabolism metabolites Metabolome metabolomics Multivariate Analysis obesity Obesity - metabolism Obesity - urine Pharmacology. Drug treatments Spectrometry, Mass, Electrospray Ionization - methods sulfates systolic blood pressure testosterone ultra-performance liquid chromatography UPLC/Q-TOF MS urine young adults Young obese men |
| title | Metabolic profiling of urine in young obese men using ultra performance liquid chromatography and Q-TOF mass spectrometry (UPLC/Q-TOF MS) |
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