Determination of ganciclovir in human plasma by ultra performance liquid chromatography–UV detection
Implement a sensitive UHPLC method for the assay of ganciclovir in human plasma. We developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was ma...
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| Veröffentlicht in: | Clinical biochemistry 2012-03, Vol.45 (4-5), p.309-314 |
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| container_title | Clinical biochemistry |
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| creator | Padullés, A. Colom, H. Armendariz, Y. Cerezo, G. Caldes, A. Pou, L. Torras, J. Grinyó, J.M. Lloberas, N. |
| description | Implement a sensitive UHPLC method for the assay of ganciclovir in human plasma.
We developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was made with respect to analysis time, efficiency and sensitivity.
Correlation coefficients (r) of the calibration curves ranged from 0.999744 to 0.999784. Within-day and between-day imprecision and inaccuracy, specificity and recovery were also evaluated for validation. The method was precise and accurate and the retention time was 0.7min. The calibration curves were linear between 0.5 and 30μg/mL. There was a good correlation between HPLC and UHPLC techniques.
We developed a method that is currently applied in a clinical study assessing GCV plasma concentration variability after ganciclovir and valganciclovir administration.
► A method assessing GCV plasma concentration after ganciclovir administration. ► Pharmacokinetic study in renal transplantation patients. ► An approach allowing dose adjustment to achieve a target plasma exposure. ► GCV determination to improve outcome in SOT patients infected by CMV. ► Plasma samples were determined by HPLC and UHPLC and both results were correlated. |
| doi_str_mv | 10.1016/j.clinbiochem.2011.12.014 |
| format | Article |
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We developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was made with respect to analysis time, efficiency and sensitivity.
Correlation coefficients (r) of the calibration curves ranged from 0.999744 to 0.999784. Within-day and between-day imprecision and inaccuracy, specificity and recovery were also evaluated for validation. The method was precise and accurate and the retention time was 0.7min. The calibration curves were linear between 0.5 and 30μg/mL. There was a good correlation between HPLC and UHPLC techniques.
We developed a method that is currently applied in a clinical study assessing GCV plasma concentration variability after ganciclovir and valganciclovir administration.
► A method assessing GCV plasma concentration after ganciclovir administration. ► Pharmacokinetic study in renal transplantation patients. ► An approach allowing dose adjustment to achieve a target plasma exposure. ► GCV determination to improve outcome in SOT patients infected by CMV. ► Plasma samples were determined by HPLC and UHPLC and both results were correlated.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/j.clinbiochem.2011.12.014</identifier><identifier>PMID: 22245106</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antiviral agents ; Antiviral Agents - blood ; Biological and medical sciences ; Calibration ; Chromatography, High Pressure Liquid ; Drug Stability ; Ganciclovir ; Ganciclovir - blood ; HPLC ; Humans ; Limit of Detection ; Medical sciences ; Pharmacology. Drug treatments ; Reproducibility of Results ; Spectrophotometry, Ultraviolet ; Therapeutic drug monitoring ; UHPLC ; Valganciclovir</subject><ispartof>Clinical biochemistry, 2012-03, Vol.45 (4-5), p.309-314</ispartof><rights>2012 The Canadian Society of Clinical Chemists</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-2fa2dcd6035e5d67e174a652cf62755ce2501b262640340e9657104796f4c6323</citedby><cites>FETCH-LOGICAL-c406t-2fa2dcd6035e5d67e174a652cf62755ce2501b262640340e9657104796f4c6323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.clinbiochem.2011.12.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25721110$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22245106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Padullés, A.</creatorcontrib><creatorcontrib>Colom, H.</creatorcontrib><creatorcontrib>Armendariz, Y.</creatorcontrib><creatorcontrib>Cerezo, G.</creatorcontrib><creatorcontrib>Caldes, A.</creatorcontrib><creatorcontrib>Pou, L.</creatorcontrib><creatorcontrib>Torras, J.</creatorcontrib><creatorcontrib>Grinyó, J.M.</creatorcontrib><creatorcontrib>Lloberas, N.</creatorcontrib><title>Determination of ganciclovir in human plasma by ultra performance liquid chromatography–UV detection</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>Implement a sensitive UHPLC method for the assay of ganciclovir in human plasma.
We developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was made with respect to analysis time, efficiency and sensitivity.
Correlation coefficients (r) of the calibration curves ranged from 0.999744 to 0.999784. Within-day and between-day imprecision and inaccuracy, specificity and recovery were also evaluated for validation. The method was precise and accurate and the retention time was 0.7min. The calibration curves were linear between 0.5 and 30μg/mL. There was a good correlation between HPLC and UHPLC techniques.
We developed a method that is currently applied in a clinical study assessing GCV plasma concentration variability after ganciclovir and valganciclovir administration.
► A method assessing GCV plasma concentration after ganciclovir administration. ► Pharmacokinetic study in renal transplantation patients. ► An approach allowing dose adjustment to achieve a target plasma exposure. ► GCV determination to improve outcome in SOT patients infected by CMV. ► Plasma samples were determined by HPLC and UHPLC and both results were correlated.</description><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antiviral agents</subject><subject>Antiviral Agents - blood</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Drug Stability</subject><subject>Ganciclovir</subject><subject>Ganciclovir - blood</subject><subject>HPLC</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Therapeutic drug monitoring</subject><subject>UHPLC</subject><subject>Valganciclovir</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAQxy1ERZeWV0DmgDgleCa20xzRli-pEpe2V8vr2F2vnDi1k0p74x14Q54Er3b5OHIajeY3_xn9CHkDrAYG8v2uNsGPGx_N1g41MoAasGbAn5EVXLVNhV3TPCcrxlhXdYDsnLzMeVda5FfyBTlHRC6AyRVx13a2afCjnn0caXT0QY_GmxCffKJ-pNtl0COdgs6Dpps9XcKcNJ1scjGVibE0-MfF99RsUxz0HB-Snrb7n99_3N3TvoSbQ_AlOXM6ZPvqVC_I3aePt-sv1c23z1_XH24qw5mcK3Qae9NL1ggretlaaLmWAo2T2AphLAoGG5QoOWs4s50ULTDedtJxIxtsLsi7Y-6U4uNi86wGn40NQY82Lll1KEFIJrpCdkfSpJhzsk5NyQ867RUwdbCsduofy-pgWQGqYrnsvj5dWTaD7f9s_tZagLcnQGejg0sHp_kvJ1oEAFa49ZGzxcmTt0ll422R2vtUxKk--v945xe0dKIx</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Padullés, A.</creator><creator>Colom, H.</creator><creator>Armendariz, Y.</creator><creator>Cerezo, G.</creator><creator>Caldes, A.</creator><creator>Pou, L.</creator><creator>Torras, J.</creator><creator>Grinyó, J.M.</creator><creator>Lloberas, N.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Determination of ganciclovir in human plasma by ultra performance liquid chromatography–UV detection</title><author>Padullés, A. ; Colom, H. ; Armendariz, Y. ; Cerezo, G. ; Caldes, A. ; Pou, L. ; Torras, J. ; Grinyó, J.M. ; Lloberas, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-2fa2dcd6035e5d67e174a652cf62755ce2501b262640340e9657104796f4c6323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antiviral agents</topic><topic>Antiviral Agents - blood</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Drug Stability</topic><topic>Ganciclovir</topic><topic>Ganciclovir - blood</topic><topic>HPLC</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Therapeutic drug monitoring</topic><topic>UHPLC</topic><topic>Valganciclovir</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Padullés, A.</creatorcontrib><creatorcontrib>Colom, H.</creatorcontrib><creatorcontrib>Armendariz, Y.</creatorcontrib><creatorcontrib>Cerezo, G.</creatorcontrib><creatorcontrib>Caldes, A.</creatorcontrib><creatorcontrib>Pou, L.</creatorcontrib><creatorcontrib>Torras, J.</creatorcontrib><creatorcontrib>Grinyó, J.M.</creatorcontrib><creatorcontrib>Lloberas, N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Padullés, A.</au><au>Colom, H.</au><au>Armendariz, Y.</au><au>Cerezo, G.</au><au>Caldes, A.</au><au>Pou, L.</au><au>Torras, J.</au><au>Grinyó, J.M.</au><au>Lloberas, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of ganciclovir in human plasma by ultra performance liquid chromatography–UV detection</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>45</volume><issue>4-5</issue><spage>309</spage><epage>314</epage><pages>309-314</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>Implement a sensitive UHPLC method for the assay of ganciclovir in human plasma.
We developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was made with respect to analysis time, efficiency and sensitivity.
Correlation coefficients (r) of the calibration curves ranged from 0.999744 to 0.999784. Within-day and between-day imprecision and inaccuracy, specificity and recovery were also evaluated for validation. The method was precise and accurate and the retention time was 0.7min. The calibration curves were linear between 0.5 and 30μg/mL. There was a good correlation between HPLC and UHPLC techniques.
We developed a method that is currently applied in a clinical study assessing GCV plasma concentration variability after ganciclovir and valganciclovir administration.
► A method assessing GCV plasma concentration after ganciclovir administration. ► Pharmacokinetic study in renal transplantation patients. ► An approach allowing dose adjustment to achieve a target plasma exposure. ► GCV determination to improve outcome in SOT patients infected by CMV. ► Plasma samples were determined by HPLC and UHPLC and both results were correlated.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>22245106</pmid><doi>10.1016/j.clinbiochem.2011.12.014</doi><tpages>6</tpages></addata></record> |
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| ispartof | Clinical biochemistry, 2012-03, Vol.45 (4-5), p.309-314 |
| issn | 0009-9120 1873-2933 |
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| subjects | Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviral agents Antiviral Agents - blood Biological and medical sciences Calibration Chromatography, High Pressure Liquid Drug Stability Ganciclovir Ganciclovir - blood HPLC Humans Limit of Detection Medical sciences Pharmacology. Drug treatments Reproducibility of Results Spectrophotometry, Ultraviolet Therapeutic drug monitoring UHPLC Valganciclovir |
| title | Determination of ganciclovir in human plasma by ultra performance liquid chromatography–UV detection |
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