Temporal and spatial changes in the microbial bioaerosol communities in green-waste composting
Abstract In this study, the microbial community within compost, emitted into the airstream, downwind and upwind from a composting facility was characterized and compared through phospholipid fatty acid analysis and 16S rRNA gene analysis using denaturing gradient gel electrophoresis and bar-coded py...
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| Veröffentlicht in: | FEMS microbiology ecology 2012-01, Vol.79 (1), p.229-239 |
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| creator | Pankhurst, Louise J. Whitby, Corinne Pawlett, Mark Larcombe, Lee D. McKew, Boyd Deacon, Lewis J. Morgan, Sarah L. Villa, Raffaella Drew, Gillian H. Tyrrel, Sean Pollard, Simon J.T. Coulon, Frédéric |
| description | Abstract
In this study, the microbial community within compost, emitted into the airstream, downwind and upwind from a composting facility was characterized and compared through phospholipid fatty acid analysis and 16S rRNA gene analysis using denaturing gradient gel electrophoresis and bar-coded pyrosequencing techniques. All methods used suggested that green-waste composting had a significant impact upon bioaerosol community composition. Daily variations of the on-site airborne community showed how specific site parameters such as compost process activity and meteorological conditions affect bioaerosol communities, although more data are required to qualify and quantify the causes for these variations. A notable feature was the dominance of Pseudomonas in downwind samples, suggesting that this genus can disperse downwind in elevated abundances. Thirty-nine phylotypes were homologous to plant or human phylotypes containing pathogens and were found within compost, on-site and downwind microbial communities. Although the significance of this finding in terms of potential health impact was beyond the scope of this study, it clearly illustrated the potential of molecular techniques to improve our understanding of the impact that green-waste composting emissions may have on the human health. |
| doi_str_mv | 10.1111/j.1574-6941.2011.01210.x |
| format | Article |
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In this study, the microbial community within compost, emitted into the airstream, downwind and upwind from a composting facility was characterized and compared through phospholipid fatty acid analysis and 16S rRNA gene analysis using denaturing gradient gel electrophoresis and bar-coded pyrosequencing techniques. All methods used suggested that green-waste composting had a significant impact upon bioaerosol community composition. Daily variations of the on-site airborne community showed how specific site parameters such as compost process activity and meteorological conditions affect bioaerosol communities, although more data are required to qualify and quantify the causes for these variations. A notable feature was the dominance of Pseudomonas in downwind samples, suggesting that this genus can disperse downwind in elevated abundances. Thirty-nine phylotypes were homologous to plant or human phylotypes containing pathogens and were found within compost, on-site and downwind microbial communities. Although the significance of this finding in terms of potential health impact was beyond the scope of this study, it clearly illustrated the potential of molecular techniques to improve our understanding of the impact that green-waste composting emissions may have on the human health.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2011.01210.x</identifier><identifier>PMID: 22029533</identifier><identifier>CODEN: FMECEZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aerosols - analysis ; Air Microbiology ; Airborne microorganisms ; Animal, plant and microbial ecology ; Applied ecology ; Bacteria - classification ; Bacteria - genetics ; Bacteria - growth & development ; bioaerosol diversity ; Biodiversity ; Biological and medical sciences ; Community composition ; compost windrow ; Composting ; Composts ; Ecology ; Ecotoxicology, biological effects of pollution ; Environmental Monitoring ; Fundamental and applied biological sciences. Psychology ; Humans ; lipid biomarkers ; Marine ecology ; Microbial activity ; Microbial ecology ; Microbiology ; pyrosequencing ; Refuse Disposal - methods ; Soil ; Soil Microbiology ; Techniques ; Various environments (extraatmospheric space, air, water) ; Wind</subject><ispartof>FEMS microbiology ecology, 2012-01, Vol.79 (1), p.229-239</ispartof><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved 2011</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4700-19932e0784cb165d8e386511833586b64cd76c99fe03dfd28aa67fbaa88cfc743</citedby><cites>FETCH-LOGICAL-c4700-19932e0784cb165d8e386511833586b64cd76c99fe03dfd28aa67fbaa88cfc743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2011.01210.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2011.01210.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25289951$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22029533$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pankhurst, Louise J.</creatorcontrib><creatorcontrib>Whitby, Corinne</creatorcontrib><creatorcontrib>Pawlett, Mark</creatorcontrib><creatorcontrib>Larcombe, Lee D.</creatorcontrib><creatorcontrib>McKew, Boyd</creatorcontrib><creatorcontrib>Deacon, Lewis J.</creatorcontrib><creatorcontrib>Morgan, Sarah L.</creatorcontrib><creatorcontrib>Villa, Raffaella</creatorcontrib><creatorcontrib>Drew, Gillian H.</creatorcontrib><creatorcontrib>Tyrrel, Sean</creatorcontrib><creatorcontrib>Pollard, Simon J.T.</creatorcontrib><creatorcontrib>Coulon, Frédéric</creatorcontrib><title>Temporal and spatial changes in the microbial bioaerosol communities in green-waste composting</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Abstract
In this study, the microbial community within compost, emitted into the airstream, downwind and upwind from a composting facility was characterized and compared through phospholipid fatty acid analysis and 16S rRNA gene analysis using denaturing gradient gel electrophoresis and bar-coded pyrosequencing techniques. All methods used suggested that green-waste composting had a significant impact upon bioaerosol community composition. Daily variations of the on-site airborne community showed how specific site parameters such as compost process activity and meteorological conditions affect bioaerosol communities, although more data are required to qualify and quantify the causes for these variations. A notable feature was the dominance of Pseudomonas in downwind samples, suggesting that this genus can disperse downwind in elevated abundances. Thirty-nine phylotypes were homologous to plant or human phylotypes containing pathogens and were found within compost, on-site and downwind microbial communities. Although the significance of this finding in terms of potential health impact was beyond the scope of this study, it clearly illustrated the potential of molecular techniques to improve our understanding of the impact that green-waste composting emissions may have on the human health.</description><subject>Aerosols - analysis</subject><subject>Air Microbiology</subject><subject>Airborne microorganisms</subject><subject>Animal, plant and microbial ecology</subject><subject>Applied ecology</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - growth & development</subject><subject>bioaerosol diversity</subject><subject>Biodiversity</subject><subject>Biological and medical sciences</subject><subject>Community composition</subject><subject>compost windrow</subject><subject>Composting</subject><subject>Composts</subject><subject>Ecology</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Environmental Monitoring</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>lipid biomarkers</subject><subject>Marine ecology</subject><subject>Microbial activity</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>pyrosequencing</subject><subject>Refuse Disposal - methods</subject><subject>Soil</subject><subject>Soil Microbiology</subject><subject>Techniques</subject><subject>Various environments (extraatmospheric space, air, water)</subject><subject>Wind</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdFr1TAUxoM43HX6L0hBxKdec5ImTR58kLHpYLKX7dWQpuldLm1Sk5Zt_72pvZugCMtLDny_7-TLOQgVgLeQz6f9FlhdlVxWsCUYYIuBZO3-Bdo8CS_RBgMXJa8kP0avU9pjDIxW-BU6JgQTySjdoB_XdhhD1H2hfVukUU8u1-ZW-51NhfPFdGuLwZkYmkVoXNA2hhQyE4Zh9m5yK7eL1vryTqfJLtIY0uT87g066nSf7NvDfYJuzs-uT7-Vl1dfL06_XJamqjEuQUpKLK5FZRrgrBWWCs4ABKVM8IZXpq25kbKzmLZdS4TWvO4arYUwnakreoI-rn3HGH7ONk1qcMnYvtfehjkpSTgwkttl8v1f5D7M0edwChjUFMtaLpRYqfzxlKLt1BjdoOODAqyWFai9WiatlkmrZQXq9wrUfba-OzwwN4Ntn4yPM8_AhwOgk9F9F7U3Lv3hck4pGWTu88rdud4-PDuAOj_7vlTZT1d_mMf_uMt_4_8CHY6wSg</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Pankhurst, Louise J.</creator><creator>Whitby, Corinne</creator><creator>Pawlett, Mark</creator><creator>Larcombe, Lee D.</creator><creator>McKew, Boyd</creator><creator>Deacon, Lewis J.</creator><creator>Morgan, Sarah L.</creator><creator>Villa, Raffaella</creator><creator>Drew, Gillian H.</creator><creator>Tyrrel, Sean</creator><creator>Pollard, Simon J.T.</creator><creator>Coulon, Frédéric</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20120101</creationdate><title>Temporal and spatial changes in the microbial bioaerosol communities in green-waste composting</title><author>Pankhurst, Louise J. ; Whitby, Corinne ; Pawlett, Mark ; Larcombe, Lee D. ; McKew, Boyd ; Deacon, Lewis J. ; Morgan, Sarah L. ; Villa, Raffaella ; Drew, Gillian H. ; Tyrrel, Sean ; Pollard, Simon J.T. ; Coulon, Frédéric</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4700-19932e0784cb165d8e386511833586b64cd76c99fe03dfd28aa67fbaa88cfc743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aerosols - analysis</topic><topic>Air Microbiology</topic><topic>Airborne microorganisms</topic><topic>Animal, plant and microbial ecology</topic><topic>Applied ecology</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - growth & development</topic><topic>bioaerosol diversity</topic><topic>Biodiversity</topic><topic>Biological and medical sciences</topic><topic>Community composition</topic><topic>compost windrow</topic><topic>Composting</topic><topic>Composts</topic><topic>Ecology</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Environmental Monitoring</topic><topic>Fundamental and applied biological sciences. 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In this study, the microbial community within compost, emitted into the airstream, downwind and upwind from a composting facility was characterized and compared through phospholipid fatty acid analysis and 16S rRNA gene analysis using denaturing gradient gel electrophoresis and bar-coded pyrosequencing techniques. All methods used suggested that green-waste composting had a significant impact upon bioaerosol community composition. Daily variations of the on-site airborne community showed how specific site parameters such as compost process activity and meteorological conditions affect bioaerosol communities, although more data are required to qualify and quantify the causes for these variations. A notable feature was the dominance of Pseudomonas in downwind samples, suggesting that this genus can disperse downwind in elevated abundances. Thirty-nine phylotypes were homologous to plant or human phylotypes containing pathogens and were found within compost, on-site and downwind microbial communities. Although the significance of this finding in terms of potential health impact was beyond the scope of this study, it clearly illustrated the potential of molecular techniques to improve our understanding of the impact that green-waste composting emissions may have on the human health.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22029533</pmid><doi>10.1111/j.1574-6941.2011.01210.x</doi><tpages>11</tpages></addata></record> |
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| subjects | Aerosols - analysis Air Microbiology Airborne microorganisms Animal, plant and microbial ecology Applied ecology Bacteria - classification Bacteria - genetics Bacteria - growth & development bioaerosol diversity Biodiversity Biological and medical sciences Community composition compost windrow Composting Composts Ecology Ecotoxicology, biological effects of pollution Environmental Monitoring Fundamental and applied biological sciences. Psychology Humans lipid biomarkers Marine ecology Microbial activity Microbial ecology Microbiology pyrosequencing Refuse Disposal - methods Soil Soil Microbiology Techniques Various environments (extraatmospheric space, air, water) Wind |
| title | Temporal and spatial changes in the microbial bioaerosol communities in green-waste composting |
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