Endocrine Disruptors, Polychlorinated Biphenyls-Induced gC1qR-dependent Apoptosis in Human Trophoblast Cell Line HTR-8/SVneo
Although an association exists between exposure to polychlorinated biphenyls (PCBs) and spontaneous miscarriage, the mechanisms underlying this phenomenon remain unclear. In this study, PCBs content in plasma was detected by gas chromatography–mass spectrometry (GC-MS) and decidua tissues were exami...
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| Veröffentlicht in: | Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2012-02, Vol.19 (2), p.181-189 |
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| container_title | Reproductive sciences (Thousand Oaks, Calif.) |
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| creator | Gu, Ping-Qing Gao, Ling-Juan Li, Li Liu, Zhu Luan, Fu-qi Peng, Yu-Zhu Guo, Xi-Rong |
| description | Although an association exists between exposure to polychlorinated biphenyls (PCBs) and spontaneous miscarriage, the mechanisms underlying this phenomenon remain unclear. In this study, PCBs content in plasma was detected by gas chromatography–mass spectrometry (GC-MS) and decidua tissues were examined for the expression of globular heads of C1q receptor (gC1qR) using Western blot in patients who underwent induced abortion and spontaneous abortion. Results showed increased PCBs content and gC1qR expression in patients who experienced spontaneous abortion. In vitro, Western blot analysis demonstrated significantly higher caspase 3 expression and apoptotic cell counts in green fluorescent protein (GFP)-gC1qR vector group. Additionally, gC1qR and caspase 3 showed decreased expression following PCBs plus gC1qR small interfering RNA (siRNA) treatment. The percentage of apoptotic cells increased in cells treated with PCBs alone or PCB plus negative siRNA. These data suggest that maternal exposure to PCBs is associated with adverse pregnancy outcomes and that upregulation of gC1qR is important for PCBs-mediated trophoblast cell apoptosis. |
| doi_str_mv | 10.1177/1933719111415866 |
| format | Article |
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In this study, PCBs content in plasma was detected by gas chromatography–mass spectrometry (GC-MS) and decidua tissues were examined for the expression of globular heads of C1q receptor (gC1qR) using Western blot in patients who underwent induced abortion and spontaneous abortion. Results showed increased PCBs content and gC1qR expression in patients who experienced spontaneous abortion. In vitro, Western blot analysis demonstrated significantly higher caspase 3 expression and apoptotic cell counts in green fluorescent protein (GFP)-gC1qR vector group. Additionally, gC1qR and caspase 3 showed decreased expression following PCBs plus gC1qR small interfering RNA (siRNA) treatment. The percentage of apoptotic cells increased in cells treated with PCBs alone or PCB plus negative siRNA. These data suggest that maternal exposure to PCBs is associated with adverse pregnancy outcomes and that upregulation of gC1qR is important for PCBs-mediated trophoblast cell apoptosis.</description><identifier>ISSN: 1933-7191</identifier><identifier>EISSN: 1933-7205</identifier><identifier>DOI: 10.1177/1933719111415866</identifier><identifier>PMID: 22101238</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Abortion, Spontaneous - blood ; Abortion, Spontaneous - chemically induced ; Adult ; Apoptosis - drug effects ; Carrier Proteins - metabolism ; Cell Line ; China ; Embryology ; Endocrine Disruptors - blood ; Endocrine Disruptors - toxicity ; Female ; Humans ; Medicine & Public Health ; Mitochondrial Proteins - metabolism ; Obstetrics/Perinatology/Midwifery ; Original Articles ; Polychlorinated Biphenyls - blood ; Polychlorinated Biphenyls - toxicity ; Pregnancy ; Pregnancy Proteins - metabolism ; Reproductive Medicine ; Trophoblasts - drug effects ; Trophoblasts - metabolism</subject><ispartof>Reproductive sciences (Thousand Oaks, Calif.), 2012-02, Vol.19 (2), p.181-189</ispartof><rights>Society for Gynecologic Investigation 2012</rights><rights>Society for Reproductive Investigation 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-ea27c15c57561ad160f88900f135bb0ed52ab678f2dbdc8d86141f3218337be3</citedby><cites>FETCH-LOGICAL-c378t-ea27c15c57561ad160f88900f135bb0ed52ab678f2dbdc8d86141f3218337be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1933719111415866$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1933719111415866$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21799,27903,27904,41467,42536,43600,43601,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22101238$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Ping-Qing</creatorcontrib><creatorcontrib>Gao, Ling-Juan</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Liu, Zhu</creatorcontrib><creatorcontrib>Luan, Fu-qi</creatorcontrib><creatorcontrib>Peng, Yu-Zhu</creatorcontrib><creatorcontrib>Guo, Xi-Rong</creatorcontrib><title>Endocrine Disruptors, Polychlorinated Biphenyls-Induced gC1qR-dependent Apoptosis in Human Trophoblast Cell Line HTR-8/SVneo</title><title>Reproductive sciences (Thousand Oaks, Calif.)</title><addtitle>Reprod. Sci</addtitle><addtitle>Reprod Sci</addtitle><description>Although an association exists between exposure to polychlorinated biphenyls (PCBs) and spontaneous miscarriage, the mechanisms underlying this phenomenon remain unclear. In this study, PCBs content in plasma was detected by gas chromatography–mass spectrometry (GC-MS) and decidua tissues were examined for the expression of globular heads of C1q receptor (gC1qR) using Western blot in patients who underwent induced abortion and spontaneous abortion. Results showed increased PCBs content and gC1qR expression in patients who experienced spontaneous abortion. In vitro, Western blot analysis demonstrated significantly higher caspase 3 expression and apoptotic cell counts in green fluorescent protein (GFP)-gC1qR vector group. Additionally, gC1qR and caspase 3 showed decreased expression following PCBs plus gC1qR small interfering RNA (siRNA) treatment. The percentage of apoptotic cells increased in cells treated with PCBs alone or PCB plus negative siRNA. These data suggest that maternal exposure to PCBs is associated with adverse pregnancy outcomes and that upregulation of gC1qR is important for PCBs-mediated trophoblast cell apoptosis.</description><subject>Abortion, Spontaneous - blood</subject><subject>Abortion, Spontaneous - chemically induced</subject><subject>Adult</subject><subject>Apoptosis - drug effects</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Line</subject><subject>China</subject><subject>Embryology</subject><subject>Endocrine Disruptors - blood</subject><subject>Endocrine Disruptors - toxicity</subject><subject>Female</subject><subject>Humans</subject><subject>Medicine & Public Health</subject><subject>Mitochondrial Proteins - metabolism</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Original Articles</subject><subject>Polychlorinated Biphenyls - blood</subject><subject>Polychlorinated Biphenyls - toxicity</subject><subject>Pregnancy</subject><subject>Pregnancy Proteins - metabolism</subject><subject>Reproductive Medicine</subject><subject>Trophoblasts - drug effects</subject><subject>Trophoblasts - metabolism</subject><issn>1933-7191</issn><issn>1933-7205</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkDFP6zAUhS0EAh6wMyFvLOTh69SJM0Lpo0iVQFCxRo590waldrCToRI_HlctDG9ATLbu-c7x9SHkHNhfgDy_hiJNcygAYARCZtkeOd6Mkpwzsf91j_oR-RPCG2NiVHB5SI44BwY8lcfkY2KN076xSO-a4Ieudz5c0SfXrvWydVFQPRp623RLtOs2JA_WDDpOFmN4f04MdmgN2p7edC56QxNoY-l0WClL5951S1e1KvR0jG1LZ5tnpvPnRF6_vFp0p-SgVm3As915Qub_JvPxNJk93j-Mb2aJTnPZJ6h4rkFokYsMlIGM1VIWjNWQiqpiaARXVZbLmpvKaGlkFuuoUw4ytlNhekIut7Gdd-8Dhr5cNUHHhVTcYQhlwTMQMGIskmxLau9C8FiXnW9Wyq9LYOWm8fL_xqPlYhc-VCs034aviiMAWyBEyS7Ql29u8Db-96fQZOdRC_wF_wkyz5eI</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Gu, Ping-Qing</creator><creator>Gao, Ling-Juan</creator><creator>Li, Li</creator><creator>Liu, Zhu</creator><creator>Luan, Fu-qi</creator><creator>Peng, Yu-Zhu</creator><creator>Guo, Xi-Rong</creator><general>SAGE Publications</general><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120201</creationdate><title>Endocrine Disruptors, Polychlorinated Biphenyls-Induced gC1qR-dependent Apoptosis in Human Trophoblast Cell Line HTR-8/SVneo</title><author>Gu, Ping-Qing ; Gao, Ling-Juan ; Li, Li ; Liu, Zhu ; Luan, Fu-qi ; Peng, Yu-Zhu ; Guo, Xi-Rong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-ea27c15c57561ad160f88900f135bb0ed52ab678f2dbdc8d86141f3218337be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Abortion, Spontaneous - blood</topic><topic>Abortion, Spontaneous - chemically induced</topic><topic>Adult</topic><topic>Apoptosis - drug effects</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Line</topic><topic>China</topic><topic>Embryology</topic><topic>Endocrine Disruptors - blood</topic><topic>Endocrine Disruptors - toxicity</topic><topic>Female</topic><topic>Humans</topic><topic>Medicine & Public Health</topic><topic>Mitochondrial Proteins - metabolism</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Original Articles</topic><topic>Polychlorinated Biphenyls - blood</topic><topic>Polychlorinated Biphenyls - toxicity</topic><topic>Pregnancy</topic><topic>Pregnancy Proteins - metabolism</topic><topic>Reproductive Medicine</topic><topic>Trophoblasts - drug effects</topic><topic>Trophoblasts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Ping-Qing</creatorcontrib><creatorcontrib>Gao, Ling-Juan</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Liu, Zhu</creatorcontrib><creatorcontrib>Luan, Fu-qi</creatorcontrib><creatorcontrib>Peng, Yu-Zhu</creatorcontrib><creatorcontrib>Guo, Xi-Rong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Ping-Qing</au><au>Gao, Ling-Juan</au><au>Li, Li</au><au>Liu, Zhu</au><au>Luan, Fu-qi</au><au>Peng, Yu-Zhu</au><au>Guo, Xi-Rong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endocrine Disruptors, Polychlorinated Biphenyls-Induced gC1qR-dependent Apoptosis in Human Trophoblast Cell Line HTR-8/SVneo</atitle><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle><stitle>Reprod. 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| subjects | Abortion, Spontaneous - blood Abortion, Spontaneous - chemically induced Adult Apoptosis - drug effects Carrier Proteins - metabolism Cell Line China Embryology Endocrine Disruptors - blood Endocrine Disruptors - toxicity Female Humans Medicine & Public Health Mitochondrial Proteins - metabolism Obstetrics/Perinatology/Midwifery Original Articles Polychlorinated Biphenyls - blood Polychlorinated Biphenyls - toxicity Pregnancy Pregnancy Proteins - metabolism Reproductive Medicine Trophoblasts - drug effects Trophoblasts - metabolism |
| title | Endocrine Disruptors, Polychlorinated Biphenyls-Induced gC1qR-dependent Apoptosis in Human Trophoblast Cell Line HTR-8/SVneo |
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