Detection of single-nucleotide polymorphisms with novel leaky surface acoustic wave biosensors, DNA ligation and enzymatic signal amplification

► We have developed a new technique based on a LSAW biosensor, enzymatic DNA ligation and enzymatic signal amplification for the detection of SNPs. ► Routine detection conditions are optimized. ► The detection range is from 10−6mol/L to 10−12mol/L (ΔP=1.853lgC+22.235, r=0.976). ► A single-point muta...

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Veröffentlicht in:	Biosensors & bioelectronics 2012-03, Vol.33 (1), p.274-278
Hauptverfasser:	Xu, Qinghua, Chang, Kai, Lu, Weiping, Chen, Wei, Ding, Yi, Jia, Shuangrong, Zhang, Kejun, Li, Fake, Shi, Jianfeng, Cao, Liang, Deng, Shaoli, Chen, Ming
Format:	Artikel
Sprache:	eng
Schlagworte:	3,3'-Diaminobenzidine - chemistry Bacterial Proteins - chemistry Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Biotin - analogs & derivatives Biotin - chemistry DNA - chemistry DNA ligation Fundamental and applied biological sciences. Psychology Horseradish Peroxidase - chemistry Humans Hydrogen-Ion Concentration Leak surface acoustic wave biosensor Methods. Procedures. Technologies Polymorphism, Single Nucleotide Sensitivity and Specificity Single nucleotide polymorphism Sound Various methods and equipments
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