Role of rabbit prostate granules on sperm viability and acrosome reaction evaluated with different methods
In the present study, the role of rabbit seminal granules was observed. Their influence on motility, capacitation and acrosome reaction, as well as the presence of apoptosis and the morphology of rabbit sperm, were compared in different conditions. Ejaculated sperm from five mature New Zealand White...
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| Veröffentlicht in: | Theriogenology 2012-03, Vol.77 (5), p.1021-1026 |
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| creator | Collodel, Giulia Cardinali, Raffaella Moretti, Elena Mattioli, Simona Ruggeri, Silvia Castellini, Cesare |
| description | In the present study, the role of rabbit seminal granules was observed. Their influence on motility, capacitation and acrosome reaction, as well as the presence of apoptosis and the morphology of rabbit sperm, were compared in different conditions. Ejaculated sperm from five mature New Zealand White rabbit bucks during three series of collections were studied, comparing raw semen, Percoll-selected sperm and Percoll-selected sperm plus prostate granules. We observed sperm motility kinetic traits by computer-assisted sperm analyzer (CASA) analysis in each sample. Acrosome status was evaluated by FITC-labeled Pisum sativum Agglutinin staining and chlortetracycline fluorescence assay, phosphatidylserine translocation was determined by AnnexinV/Propidium iodide assay and sperm morphology was studied using transmission electron microscopy (TEM). All traits were observed after 30 min incubation at 37 °C in 5% CO2. Data showed that sperm motility and viability markedly improved in the presence of prostate granules, whereas capacitation, acrosome reaction and phosphatidylserine translocation were lowered. TEM confirmed these results. In conclusion, the role of granules was confirmed in synchronizing sperm capacitation and acrosome reaction with egg availability; indeed, rabbit ovulation occurs only 6 to 10 h after mating. |
| doi_str_mv | 10.1016/j.theriogenology.2011.09.019 |
| format | Article |
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Their influence on motility, capacitation and acrosome reaction, as well as the presence of apoptosis and the morphology of rabbit sperm, were compared in different conditions. Ejaculated sperm from five mature New Zealand White rabbit bucks during three series of collections were studied, comparing raw semen, Percoll-selected sperm and Percoll-selected sperm plus prostate granules. We observed sperm motility kinetic traits by computer-assisted sperm analyzer (CASA) analysis in each sample. Acrosome status was evaluated by FITC-labeled Pisum sativum Agglutinin staining and chlortetracycline fluorescence assay, phosphatidylserine translocation was determined by AnnexinV/Propidium iodide assay and sperm morphology was studied using transmission electron microscopy (TEM). All traits were observed after 30 min incubation at 37 °C in 5% CO2. Data showed that sperm motility and viability markedly improved in the presence of prostate granules, whereas capacitation, acrosome reaction and phosphatidylserine translocation were lowered. TEM confirmed these results. In conclusion, the role of granules was confirmed in synchronizing sperm capacitation and acrosome reaction with egg availability; indeed, rabbit ovulation occurs only 6 to 10 h after mating.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2011.09.019</identifier><identifier>PMID: 22115805</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>acrosome ; acrosome reaction ; Acrosome Reaction - physiology ; agglutinins ; Animals ; Annexin V/PI ; bucks ; carbon dioxide ; CASA ; Cell Survival - physiology ; CTC ; Cytoplasmic Granules - chemistry ; Cytoplasmic Granules - physiology ; eggs ; fluorescence ; granules ; iodides ; Male ; Microscopy, Electron, Transmission ; New Zealand White rabbit ; ovulation ; Pisum sativum ; Prostate - ultrastructure ; Rabbit sperm ; Rabbits ; Semen ; Seminal droplets ; sperm capacitation ; Sperm Capacitation - physiology ; sperm motility ; Sperm Motility - physiology ; Spermatozoa - cytology ; Spermatozoa - physiology ; TEM ; transmission electron microscopy ; viability</subject><ispartof>Theriogenology, 2012-03, Vol.77 (5), p.1021-1026</ispartof><rights>2012 Elsevier Inc.</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-7044c6e1295041c7975b071037ec57f78a28e50e18381b918cf69a9ca4e5b84f3</citedby><cites>FETCH-LOGICAL-c409t-7044c6e1295041c7975b071037ec57f78a28e50e18381b918cf69a9ca4e5b84f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2011.09.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22115805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Collodel, Giulia</creatorcontrib><creatorcontrib>Cardinali, Raffaella</creatorcontrib><creatorcontrib>Moretti, Elena</creatorcontrib><creatorcontrib>Mattioli, Simona</creatorcontrib><creatorcontrib>Ruggeri, Silvia</creatorcontrib><creatorcontrib>Castellini, Cesare</creatorcontrib><title>Role of rabbit prostate granules on sperm viability and acrosome reaction evaluated with different methods</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>In the present study, the role of rabbit seminal granules was observed. Their influence on motility, capacitation and acrosome reaction, as well as the presence of apoptosis and the morphology of rabbit sperm, were compared in different conditions. Ejaculated sperm from five mature New Zealand White rabbit bucks during three series of collections were studied, comparing raw semen, Percoll-selected sperm and Percoll-selected sperm plus prostate granules. We observed sperm motility kinetic traits by computer-assisted sperm analyzer (CASA) analysis in each sample. Acrosome status was evaluated by FITC-labeled Pisum sativum Agglutinin staining and chlortetracycline fluorescence assay, phosphatidylserine translocation was determined by AnnexinV/Propidium iodide assay and sperm morphology was studied using transmission electron microscopy (TEM). All traits were observed after 30 min incubation at 37 °C in 5% CO2. Data showed that sperm motility and viability markedly improved in the presence of prostate granules, whereas capacitation, acrosome reaction and phosphatidylserine translocation were lowered. TEM confirmed these results. In conclusion, the role of granules was confirmed in synchronizing sperm capacitation and acrosome reaction with egg availability; indeed, rabbit ovulation occurs only 6 to 10 h after mating.</description><subject>acrosome</subject><subject>acrosome reaction</subject><subject>Acrosome Reaction - physiology</subject><subject>agglutinins</subject><subject>Animals</subject><subject>Annexin V/PI</subject><subject>bucks</subject><subject>carbon dioxide</subject><subject>CASA</subject><subject>Cell Survival - physiology</subject><subject>CTC</subject><subject>Cytoplasmic Granules - chemistry</subject><subject>Cytoplasmic Granules - physiology</subject><subject>eggs</subject><subject>fluorescence</subject><subject>granules</subject><subject>iodides</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>New Zealand White rabbit</subject><subject>ovulation</subject><subject>Pisum sativum</subject><subject>Prostate - ultrastructure</subject><subject>Rabbit sperm</subject><subject>Rabbits</subject><subject>Semen</subject><subject>Seminal droplets</subject><subject>sperm capacitation</subject><subject>Sperm Capacitation - physiology</subject><subject>sperm motility</subject><subject>Sperm Motility - physiology</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - physiology</subject><subject>TEM</subject><subject>transmission electron microscopy</subject><subject>viability</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-L1DAUx4Mo7rj6L2gOgqfW95q2acCLLK4KC4K64C2k6etMhrYZk3Rk_nszzCp48_Qun-_78XmMvUYoEbB9uy_TjoLzW1r85LensgLEElQJqB6xDXZSFaIS-JhtAJQoWoU_rtizGPcAINoWn7KrqkJsOmg2bP_VT8T9yIPpe5f4IfiYTCK-DWZZJ4rcLzweKMz86EzvJpdO3CwDNzaTfiYeyNjkMkVHM605OvBfLu344MaRAi2Jz5R2fojP2ZPRTJFePNRrdn_74fvNp-Luy8fPN-_vCluDSoWEurYtYaUaqNFKJZseJIKQZBs5ys5UHTVA2IkOe4WdHVtllDU1NX1Xj-Kavbn0zbf8XCkmPbtoaZrMQn6NWlVCNUJ2IpPvLuT5lhho1IfgZhNOGkGfZeu9_le2PsvWoHSWneMvHwat_UzD3_Afuxl4dQFG47XZBhf1_bfcoT5_QmYkE7cXgrKQo6Ogo3W0WBpcIJv04N3_7fIbkJekFw</recordid><startdate>20120315</startdate><enddate>20120315</enddate><creator>Collodel, Giulia</creator><creator>Cardinali, Raffaella</creator><creator>Moretti, Elena</creator><creator>Mattioli, Simona</creator><creator>Ruggeri, Silvia</creator><creator>Castellini, Cesare</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120315</creationdate><title>Role of rabbit prostate granules on sperm viability and acrosome reaction evaluated with different methods</title><author>Collodel, Giulia ; Cardinali, Raffaella ; Moretti, Elena ; Mattioli, Simona ; Ruggeri, Silvia ; Castellini, Cesare</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-7044c6e1295041c7975b071037ec57f78a28e50e18381b918cf69a9ca4e5b84f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>acrosome</topic><topic>acrosome reaction</topic><topic>Acrosome Reaction - physiology</topic><topic>agglutinins</topic><topic>Animals</topic><topic>Annexin V/PI</topic><topic>bucks</topic><topic>carbon dioxide</topic><topic>CASA</topic><topic>Cell Survival - physiology</topic><topic>CTC</topic><topic>Cytoplasmic Granules - chemistry</topic><topic>Cytoplasmic Granules - physiology</topic><topic>eggs</topic><topic>fluorescence</topic><topic>granules</topic><topic>iodides</topic><topic>Male</topic><topic>Microscopy, Electron, Transmission</topic><topic>New Zealand White rabbit</topic><topic>ovulation</topic><topic>Pisum sativum</topic><topic>Prostate - ultrastructure</topic><topic>Rabbit sperm</topic><topic>Rabbits</topic><topic>Semen</topic><topic>Seminal droplets</topic><topic>sperm capacitation</topic><topic>Sperm Capacitation - physiology</topic><topic>sperm motility</topic><topic>Sperm Motility - physiology</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - physiology</topic><topic>TEM</topic><topic>transmission electron microscopy</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Collodel, Giulia</creatorcontrib><creatorcontrib>Cardinali, Raffaella</creatorcontrib><creatorcontrib>Moretti, Elena</creatorcontrib><creatorcontrib>Mattioli, Simona</creatorcontrib><creatorcontrib>Ruggeri, Silvia</creatorcontrib><creatorcontrib>Castellini, Cesare</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Collodel, Giulia</au><au>Cardinali, Raffaella</au><au>Moretti, Elena</au><au>Mattioli, Simona</au><au>Ruggeri, Silvia</au><au>Castellini, Cesare</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of rabbit prostate granules on sperm viability and acrosome reaction evaluated with different methods</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2012-03-15</date><risdate>2012</risdate><volume>77</volume><issue>5</issue><spage>1021</spage><epage>1026</epage><pages>1021-1026</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>In the present study, the role of rabbit seminal granules was observed. Their influence on motility, capacitation and acrosome reaction, as well as the presence of apoptosis and the morphology of rabbit sperm, were compared in different conditions. Ejaculated sperm from five mature New Zealand White rabbit bucks during three series of collections were studied, comparing raw semen, Percoll-selected sperm and Percoll-selected sperm plus prostate granules. We observed sperm motility kinetic traits by computer-assisted sperm analyzer (CASA) analysis in each sample. Acrosome status was evaluated by FITC-labeled Pisum sativum Agglutinin staining and chlortetracycline fluorescence assay, phosphatidylserine translocation was determined by AnnexinV/Propidium iodide assay and sperm morphology was studied using transmission electron microscopy (TEM). All traits were observed after 30 min incubation at 37 °C in 5% CO2. Data showed that sperm motility and viability markedly improved in the presence of prostate granules, whereas capacitation, acrosome reaction and phosphatidylserine translocation were lowered. TEM confirmed these results. In conclusion, the role of granules was confirmed in synchronizing sperm capacitation and acrosome reaction with egg availability; indeed, rabbit ovulation occurs only 6 to 10 h after mating.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22115805</pmid><doi>10.1016/j.theriogenology.2011.09.019</doi><tpages>6</tpages></addata></record> |
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| subjects | acrosome acrosome reaction Acrosome Reaction - physiology agglutinins Animals Annexin V/PI bucks carbon dioxide CASA Cell Survival - physiology CTC Cytoplasmic Granules - chemistry Cytoplasmic Granules - physiology eggs fluorescence granules iodides Male Microscopy, Electron, Transmission New Zealand White rabbit ovulation Pisum sativum Prostate - ultrastructure Rabbit sperm Rabbits Semen Seminal droplets sperm capacitation Sperm Capacitation - physiology sperm motility Sperm Motility - physiology Spermatozoa - cytology Spermatozoa - physiology TEM transmission electron microscopy viability |
| title | Role of rabbit prostate granules on sperm viability and acrosome reaction evaluated with different methods |
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