Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst
► Novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) catalyst was prepared by a modified Watanabe method. ► The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062–14.07mM) and a low detection limit 0.031mM. ► The biosens...
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| Veröffentlicht in: | Biosensors & bioelectronics 2012-03, Vol.33 (1), p.75-81 |
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| creator | Chen, Kuan-Jung Lee, Chia-Feng Rick, John Wang, Shih-Han Liu, Chung-Chiun Hwang, Bing-Joe |
| description | ► Novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) catalyst was prepared by a modified Watanabe method. ► The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062–14.07mM) and a low detection limit 0.031mM. ► The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM−1cm−2). ► The biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability.
A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Nafion film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062–14.07mM) and a low detection limit 0.031mM. The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM−1cm−2). The Michaelis–Menten constant (Km) was determined as 3.3mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated. |
| doi_str_mv | 10.1016/j.bios.2011.12.020 |
| format | Article |
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A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Nafion film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062–14.07mM) and a low detection limit 0.031mM. The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM−1cm−2). The Michaelis–Menten constant (Km) was determined as 3.3mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2011.12.020</identifier><identifier>PMID: 22277115</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Blood Glucose - analysis ; Catalysis ; Electrochemical Techniques - methods ; Electrodes ; Fundamental and applied biological sciences. Psychology ; Glucose amperometric sensor ; Humans ; Hydrogen peroxide sensing ; Metal Nanoparticles - chemistry ; Methods. Procedures. Technologies ; Nanotubes, Carbon - chemistry ; Palladium - chemistry ; Platinum - chemistry ; Pt-based bimetallic nanoparticles (NPs) ; PtPd NPs-decorated multi walled carbon nanotube catalyst ; Reproducibility of Results ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2012-03, Vol.33 (1), p.75-81</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-41ebb43386008464af8ace51a5b8127683254f59ef7eb91258b5b5e11eebd1733</citedby><cites>FETCH-LOGICAL-c385t-41ebb43386008464af8ace51a5b8127683254f59ef7eb91258b5b5e11eebd1733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2011.12.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25646274$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22277115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Kuan-Jung</creatorcontrib><creatorcontrib>Lee, Chia-Feng</creatorcontrib><creatorcontrib>Rick, John</creatorcontrib><creatorcontrib>Wang, Shih-Han</creatorcontrib><creatorcontrib>Liu, Chung-Chiun</creatorcontrib><creatorcontrib>Hwang, Bing-Joe</creatorcontrib><title>Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>► Novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) catalyst was prepared by a modified Watanabe method. ► The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062–14.07mM) and a low detection limit 0.031mM. ► The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM−1cm−2). ► The biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability.
A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Nafion film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062–14.07mM) and a low detection limit 0.031mM. The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM−1cm−2). The Michaelis–Menten constant (Km) was determined as 3.3mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Blood Glucose - analysis</subject><subject>Catalysis</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrodes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose amperometric sensor</subject><subject>Humans</subject><subject>Hydrogen peroxide sensing</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Methods. Procedures. Technologies</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Palladium - chemistry</subject><subject>Platinum - chemistry</subject><subject>Pt-based bimetallic nanoparticles (NPs)</subject><subject>PtPd NPs-decorated multi walled carbon nanotube catalyst</subject><subject>Reproducibility of Results</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2L1TAUhosoznX0D7iQbMRVaz6atAU3MjgqDDgLXYeT9FRySZuapDPMb_HPmnLv6M5VOOR53oTzVtVrRhtGmXp_bIwLqeGUsYbxhnL6pDqwvhN1y4V8Wh3oIFUtlRIX1YuUjpTSjg30eXXBOe86xuSh-n0NJjoL2YWFwDISWFf_OIeJwLxiDDPmApGffrMhIdmfxSWFSAwkHMmukiXcoSe3-XYs90UAX3LIAktYIWZnPZIRbYiQizFvPrv6vjBlsBBNidjRvBksc5EfUn5ZPZvAJ3x1Pi-rH9efvl99qW--ff569fGmtqKXuW4ZGtMK0StK-1a1MPVgUTKQpme8U73gsp3kgFOHZmBc9kYaiYwhmpF1QlxW7065awy_NkxZzy5Z9B4WDFvSAxeDZFzxQvITaWNIKeKk1-hmiA-aUb13oo96X47eO9GM69JJkd6c4zcz4_hXeSyhAG_PACQLfoqwWJf-cVK1indt4T6cOCzLuHMYdbIOF4uji2izHoP73z_-AL2mrbo</recordid><startdate>20120315</startdate><enddate>20120315</enddate><creator>Chen, Kuan-Jung</creator><creator>Lee, Chia-Feng</creator><creator>Rick, John</creator><creator>Wang, Shih-Han</creator><creator>Liu, Chung-Chiun</creator><creator>Hwang, Bing-Joe</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120315</creationdate><title>Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst</title><author>Chen, Kuan-Jung ; Lee, Chia-Feng ; Rick, John ; Wang, Shih-Han ; Liu, Chung-Chiun ; Hwang, Bing-Joe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-41ebb43386008464af8ace51a5b8127683254f59ef7eb91258b5b5e11eebd1733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Blood Glucose - analysis</topic><topic>Catalysis</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrodes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose amperometric sensor</topic><topic>Humans</topic><topic>Hydrogen peroxide sensing</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Methods. Procedures. Technologies</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Palladium - chemistry</topic><topic>Platinum - chemistry</topic><topic>Pt-based bimetallic nanoparticles (NPs)</topic><topic>PtPd NPs-decorated multi walled carbon nanotube catalyst</topic><topic>Reproducibility of Results</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Kuan-Jung</creatorcontrib><creatorcontrib>Lee, Chia-Feng</creatorcontrib><creatorcontrib>Rick, John</creatorcontrib><creatorcontrib>Wang, Shih-Han</creatorcontrib><creatorcontrib>Liu, Chung-Chiun</creatorcontrib><creatorcontrib>Hwang, Bing-Joe</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Kuan-Jung</au><au>Lee, Chia-Feng</au><au>Rick, John</au><au>Wang, Shih-Han</au><au>Liu, Chung-Chiun</au><au>Hwang, Bing-Joe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2012-03-15</date><risdate>2012</risdate><volume>33</volume><issue>1</issue><spage>75</spage><epage>81</epage><pages>75-81</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>► Novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) catalyst was prepared by a modified Watanabe method. ► The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062–14.07mM) and a low detection limit 0.031mM. ► The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM−1cm−2). ► The biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability.
A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Nafion film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062–14.07mM) and a low detection limit 0.031mM. The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM−1cm−2). The Michaelis–Menten constant (Km) was determined as 3.3mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>22277115</pmid><doi>10.1016/j.bios.2011.12.020</doi><tpages>7</tpages></addata></record> |
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| subjects | Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Blood Glucose - analysis Catalysis Electrochemical Techniques - methods Electrodes Fundamental and applied biological sciences. Psychology Glucose amperometric sensor Humans Hydrogen peroxide sensing Metal Nanoparticles - chemistry Methods. Procedures. Technologies Nanotubes, Carbon - chemistry Palladium - chemistry Platinum - chemistry Pt-based bimetallic nanoparticles (NPs) PtPd NPs-decorated multi walled carbon nanotube catalyst Reproducibility of Results Various methods and equipments |
| title | Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst |
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