Proteomics Profiling of Human Embryonic Stem Cells in the Early Differentiation Stage
The regulatory pathways responsible for maintaining human embryonic stem cells (hESCs) in an undifferentiated state have yet to be elucidated. Since these pathways are thought to be governed by complex protein cues, deciphering the changes that occur in the proteomes of the ESCs during differentiati...
Gespeichert in:
Veröffentlicht in: | Stem cell reviews 2012-03, Vol.8 (1), p.137-149 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 149 |
---|---|
container_issue | 1 |
container_start_page | 137 |
container_title | Stem cell reviews |
container_volume | 8 |
creator | Novak, Atara Amit, Michal Ziv, Tamar Segev, Hanna Fishman, Bettina Admon, Arie Itskovitz-Eldor, Joseph |
description | The regulatory pathways responsible for maintaining human embryonic stem cells (hESCs) in an undifferentiated state have yet to be elucidated. Since these pathways are thought to be governed by complex protein cues, deciphering the changes that occur in the proteomes of the ESCs during differentiation is important for understanding the expansion and differentiation processes involved. In this study, we present the first quantitative comparison of the hESC protein profile in the undifferentiated and early differentiated states. We used iTRAQ (isobaric tags for relative and absolute quantification) labeling combined with two dimensional capillary chromatography coupled with tandem mass spectrometry (μLC-MS/MS) to achieve comparative proteomics of hESCs at the undifferentiated stage, and at 6, 48, and 72 h after initiation of differentiation. In addition, two dimensional electrophoresis (2-DE) was performed on differentiating hESCs at eleven points of time during the first 72 h of differentiation. The results indicate that during the first 48 h of hESC differentiation, many processes are initiated and are later reversed, including chromatin remodeling, heterochromatin spreading, a decrease in transcription and translation, a decrease in glycolytic proteins and cytoskeleton remodeling, and a decrease in focal and cell adhesion. Only 72 h after differentiation induction did the expression of the homeobox prox1 protein increase, indicating the beginning of developmental processes. |
doi_str_mv | 10.1007/s12015-011-9286-y |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_923579540</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>923579540</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-7f77b5a0b0d53a9a5f14fd6080c290c721320b5945f93a42b55dbe8087128ca03</originalsourceid><addsrcrecordid>eNp9kU1P3DAQhq0KVD7aH9ALsriUS-j4a20fq2ULSEggtZwtJ2svRolN7eSQf4-3S0GqVE4eyc-8M6MHoS8EzgmA_FYIBSIaIKTRVC2a-QM6JEKoZqGA7_2poVGaswN0VMojAFNckY_ogBLJKGh6iO7vchpdGkJXcC196EPc4OTx1TTYiFdDm-cUQ4d_jm7AS9f3BYeIxweHVzb3M74I3rvs4hjsGFKsnN24T2jf2764zy_vMbr_sfq1vGpubi-vl99vmo4DGxvppWyFhRbWgllthSfcrxegoKMaOklJ3bIVmguvmeW0FWLdOgVKEqo6C-wYfd3lPuX0e3JlNEMoXV3SRpemYjRlQmrBt-TZu2SdxBQRGlRFT_9BH9OUY71jm0cJXwhZIbKDupxKyc6bpxwGm2dDwGzlmJ0cU-WYrRwz156Tl-CpHdz6teOvjQrQHVDqV9y4_Db5_6nPKQyYZA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>923214657</pqid></control><display><type>article</type><title>Proteomics Profiling of Human Embryonic Stem Cells in the Early Differentiation Stage</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Novak, Atara ; Amit, Michal ; Ziv, Tamar ; Segev, Hanna ; Fishman, Bettina ; Admon, Arie ; Itskovitz-Eldor, Joseph</creator><creatorcontrib>Novak, Atara ; Amit, Michal ; Ziv, Tamar ; Segev, Hanna ; Fishman, Bettina ; Admon, Arie ; Itskovitz-Eldor, Joseph</creatorcontrib><description>The regulatory pathways responsible for maintaining human embryonic stem cells (hESCs) in an undifferentiated state have yet to be elucidated. Since these pathways are thought to be governed by complex protein cues, deciphering the changes that occur in the proteomes of the ESCs during differentiation is important for understanding the expansion and differentiation processes involved. In this study, we present the first quantitative comparison of the hESC protein profile in the undifferentiated and early differentiated states. We used iTRAQ (isobaric tags for relative and absolute quantification) labeling combined with two dimensional capillary chromatography coupled with tandem mass spectrometry (μLC-MS/MS) to achieve comparative proteomics of hESCs at the undifferentiated stage, and at 6, 48, and 72 h after initiation of differentiation. In addition, two dimensional electrophoresis (2-DE) was performed on differentiating hESCs at eleven points of time during the first 72 h of differentiation. The results indicate that during the first 48 h of hESC differentiation, many processes are initiated and are later reversed, including chromatin remodeling, heterochromatin spreading, a decrease in transcription and translation, a decrease in glycolytic proteins and cytoskeleton remodeling, and a decrease in focal and cell adhesion. Only 72 h after differentiation induction did the expression of the homeobox prox1 protein increase, indicating the beginning of developmental processes.</description><identifier>ISSN: 1550-8943</identifier><identifier>ISSN: 2629-3269</identifier><identifier>EISSN: 1558-6804</identifier><identifier>EISSN: 2629-3277</identifier><identifier>DOI: 10.1007/s12015-011-9286-y</identifier><identifier>PMID: 21732092</identifier><language>eng</language><publisher>New York: Humana Press Inc</publisher><subject>Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Cell adhesion ; Cell Biology ; Cell Differentiation ; Cell Shape ; Cells, Cultured ; Chromatin remodeling ; Chromatography ; Cytoskeleton ; Differentiation ; Electrophoresis ; Electrophoresis, Gel, Two-Dimensional ; Embryo cells ; Embryonic Stem Cells - metabolism ; Embryonic Stem Cells - physiology ; Gene Expression ; Gene Expression Profiling ; Gene Expression Regulation ; Glycolysis ; Heterochromatin ; Homeobox ; Humans ; Karyotype ; Life Sciences ; Mass spectroscopy ; Proteome - genetics ; Proteome - isolation & purification ; Proteome - metabolism ; Proteomics ; Regenerative Medicine/Tissue Engineering ; Spreading ; Stem Cells ; Tandem Mass Spectrometry ; Transcription ; Translation</subject><ispartof>Stem cell reviews, 2012-03, Vol.8 (1), p.137-149</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><rights>Springer Science+Business Media, LLC 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-7f77b5a0b0d53a9a5f14fd6080c290c721320b5945f93a42b55dbe8087128ca03</citedby><cites>FETCH-LOGICAL-c403t-7f77b5a0b0d53a9a5f14fd6080c290c721320b5945f93a42b55dbe8087128ca03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21732092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Novak, Atara</creatorcontrib><creatorcontrib>Amit, Michal</creatorcontrib><creatorcontrib>Ziv, Tamar</creatorcontrib><creatorcontrib>Segev, Hanna</creatorcontrib><creatorcontrib>Fishman, Bettina</creatorcontrib><creatorcontrib>Admon, Arie</creatorcontrib><creatorcontrib>Itskovitz-Eldor, Joseph</creatorcontrib><title>Proteomics Profiling of Human Embryonic Stem Cells in the Early Differentiation Stage</title><title>Stem cell reviews</title><addtitle>Stem Cell Rev and Rep</addtitle><addtitle>Stem Cell Rev Rep</addtitle><description>The regulatory pathways responsible for maintaining human embryonic stem cells (hESCs) in an undifferentiated state have yet to be elucidated. Since these pathways are thought to be governed by complex protein cues, deciphering the changes that occur in the proteomes of the ESCs during differentiation is important for understanding the expansion and differentiation processes involved. In this study, we present the first quantitative comparison of the hESC protein profile in the undifferentiated and early differentiated states. We used iTRAQ (isobaric tags for relative and absolute quantification) labeling combined with two dimensional capillary chromatography coupled with tandem mass spectrometry (μLC-MS/MS) to achieve comparative proteomics of hESCs at the undifferentiated stage, and at 6, 48, and 72 h after initiation of differentiation. In addition, two dimensional electrophoresis (2-DE) was performed on differentiating hESCs at eleven points of time during the first 72 h of differentiation. The results indicate that during the first 48 h of hESC differentiation, many processes are initiated and are later reversed, including chromatin remodeling, heterochromatin spreading, a decrease in transcription and translation, a decrease in glycolytic proteins and cytoskeleton remodeling, and a decrease in focal and cell adhesion. Only 72 h after differentiation induction did the expression of the homeobox prox1 protein increase, indicating the beginning of developmental processes.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Cell adhesion</subject><subject>Cell Biology</subject><subject>Cell Differentiation</subject><subject>Cell Shape</subject><subject>Cells, Cultured</subject><subject>Chromatin remodeling</subject><subject>Chromatography</subject><subject>Cytoskeleton</subject><subject>Differentiation</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Embryo cells</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Glycolysis</subject><subject>Heterochromatin</subject><subject>Homeobox</subject><subject>Humans</subject><subject>Karyotype</subject><subject>Life Sciences</subject><subject>Mass spectroscopy</subject><subject>Proteome - genetics</subject><subject>Proteome - isolation & purification</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Spreading</subject><subject>Stem Cells</subject><subject>Tandem Mass Spectrometry</subject><subject>Transcription</subject><subject>Translation</subject><issn>1550-8943</issn><issn>2629-3269</issn><issn>1558-6804</issn><issn>2629-3277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1P3DAQhq0KVD7aH9ALsriUS-j4a20fq2ULSEggtZwtJ2svRolN7eSQf4-3S0GqVE4eyc-8M6MHoS8EzgmA_FYIBSIaIKTRVC2a-QM6JEKoZqGA7_2poVGaswN0VMojAFNckY_ogBLJKGh6iO7vchpdGkJXcC196EPc4OTx1TTYiFdDm-cUQ4d_jm7AS9f3BYeIxweHVzb3M74I3rvs4hjsGFKsnN24T2jf2764zy_vMbr_sfq1vGpubi-vl99vmo4DGxvppWyFhRbWgllthSfcrxegoKMaOklJ3bIVmguvmeW0FWLdOgVKEqo6C-wYfd3lPuX0e3JlNEMoXV3SRpemYjRlQmrBt-TZu2SdxBQRGlRFT_9BH9OUY71jm0cJXwhZIbKDupxKyc6bpxwGm2dDwGzlmJ0cU-WYrRwz156Tl-CpHdz6teOvjQrQHVDqV9y4_Db5_6nPKQyYZA</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Novak, Atara</creator><creator>Amit, Michal</creator><creator>Ziv, Tamar</creator><creator>Segev, Hanna</creator><creator>Fishman, Bettina</creator><creator>Admon, Arie</creator><creator>Itskovitz-Eldor, Joseph</creator><general>Humana Press Inc</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Proteomics Profiling of Human Embryonic Stem Cells in the Early Differentiation Stage</title><author>Novak, Atara ; Amit, Michal ; Ziv, Tamar ; Segev, Hanna ; Fishman, Bettina ; Admon, Arie ; Itskovitz-Eldor, Joseph</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-7f77b5a0b0d53a9a5f14fd6080c290c721320b5945f93a42b55dbe8087128ca03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Cell adhesion</topic><topic>Cell Biology</topic><topic>Cell Differentiation</topic><topic>Cell Shape</topic><topic>Cells, Cultured</topic><topic>Chromatin remodeling</topic><topic>Chromatography</topic><topic>Cytoskeleton</topic><topic>Differentiation</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Embryo cells</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Glycolysis</topic><topic>Heterochromatin</topic><topic>Homeobox</topic><topic>Humans</topic><topic>Karyotype</topic><topic>Life Sciences</topic><topic>Mass spectroscopy</topic><topic>Proteome - genetics</topic><topic>Proteome - isolation & purification</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Spreading</topic><topic>Stem Cells</topic><topic>Tandem Mass Spectrometry</topic><topic>Transcription</topic><topic>Translation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Novak, Atara</creatorcontrib><creatorcontrib>Amit, Michal</creatorcontrib><creatorcontrib>Ziv, Tamar</creatorcontrib><creatorcontrib>Segev, Hanna</creatorcontrib><creatorcontrib>Fishman, Bettina</creatorcontrib><creatorcontrib>Admon, Arie</creatorcontrib><creatorcontrib>Itskovitz-Eldor, Joseph</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Stem cell reviews</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Novak, Atara</au><au>Amit, Michal</au><au>Ziv, Tamar</au><au>Segev, Hanna</au><au>Fishman, Bettina</au><au>Admon, Arie</au><au>Itskovitz-Eldor, Joseph</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomics Profiling of Human Embryonic Stem Cells in the Early Differentiation Stage</atitle><jtitle>Stem cell reviews</jtitle><stitle>Stem Cell Rev and Rep</stitle><addtitle>Stem Cell Rev Rep</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>8</volume><issue>1</issue><spage>137</spage><epage>149</epage><pages>137-149</pages><issn>1550-8943</issn><issn>2629-3269</issn><eissn>1558-6804</eissn><eissn>2629-3277</eissn><abstract>The regulatory pathways responsible for maintaining human embryonic stem cells (hESCs) in an undifferentiated state have yet to be elucidated. Since these pathways are thought to be governed by complex protein cues, deciphering the changes that occur in the proteomes of the ESCs during differentiation is important for understanding the expansion and differentiation processes involved. In this study, we present the first quantitative comparison of the hESC protein profile in the undifferentiated and early differentiated states. We used iTRAQ (isobaric tags for relative and absolute quantification) labeling combined with two dimensional capillary chromatography coupled with tandem mass spectrometry (μLC-MS/MS) to achieve comparative proteomics of hESCs at the undifferentiated stage, and at 6, 48, and 72 h after initiation of differentiation. In addition, two dimensional electrophoresis (2-DE) was performed on differentiating hESCs at eleven points of time during the first 72 h of differentiation. The results indicate that during the first 48 h of hESC differentiation, many processes are initiated and are later reversed, including chromatin remodeling, heterochromatin spreading, a decrease in transcription and translation, a decrease in glycolytic proteins and cytoskeleton remodeling, and a decrease in focal and cell adhesion. Only 72 h after differentiation induction did the expression of the homeobox prox1 protein increase, indicating the beginning of developmental processes.</abstract><cop>New York</cop><pub>Humana Press Inc</pub><pmid>21732092</pmid><doi>10.1007/s12015-011-9286-y</doi><tpages>13</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1550-8943 |
ispartof | Stem cell reviews, 2012-03, Vol.8 (1), p.137-149 |
issn | 1550-8943 2629-3269 1558-6804 2629-3277 |
language | eng |
recordid | cdi_proquest_miscellaneous_923579540 |
source | MEDLINE; SpringerNature Journals |
subjects | Biomedical and Life Sciences Biomedical Engineering and Bioengineering Cell adhesion Cell Biology Cell Differentiation Cell Shape Cells, Cultured Chromatin remodeling Chromatography Cytoskeleton Differentiation Electrophoresis Electrophoresis, Gel, Two-Dimensional Embryo cells Embryonic Stem Cells - metabolism Embryonic Stem Cells - physiology Gene Expression Gene Expression Profiling Gene Expression Regulation Glycolysis Heterochromatin Homeobox Humans Karyotype Life Sciences Mass spectroscopy Proteome - genetics Proteome - isolation & purification Proteome - metabolism Proteomics Regenerative Medicine/Tissue Engineering Spreading Stem Cells Tandem Mass Spectrometry Transcription Translation |
title | Proteomics Profiling of Human Embryonic Stem Cells in the Early Differentiation Stage |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T06%3A05%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Proteomics%20Profiling%20of%20Human%20Embryonic%20Stem%20Cells%20in%20the%20Early%20Differentiation%20Stage&rft.jtitle=Stem%20cell%20reviews&rft.au=Novak,%20Atara&rft.date=2012-03-01&rft.volume=8&rft.issue=1&rft.spage=137&rft.epage=149&rft.pages=137-149&rft.issn=1550-8943&rft.eissn=1558-6804&rft_id=info:doi/10.1007/s12015-011-9286-y&rft_dat=%3Cproquest_cross%3E923579540%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=923214657&rft_id=info:pmid/21732092&rfr_iscdi=true |