Ability of Cricetomys rats to detect Mycobacterium tuberculosis and discriminate it from other microorganisms
Summary Trained African giant pouched rats ( Cricetomys gambianus ) have potential for diagnosis of tuberculosis (TB). These rats target volatile compounds of Mycobacterium tuberculosis ( Mtb ) that cause TB. Mtb and nontuberculous mycobacteria (NTM) species are related to Nocardia and Rhodococcus s...
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| Veröffentlicht in: | Tuberculosis (Edinburgh, Scotland) Scotland), 2012-03, Vol.92 (2), p.182-186 |
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| creator | Mgode, Georgies F Weetjens, Bart J Cox, Christophe Jubitana, Maureen Machang’u, Robert S Lazar, Doris Weiner, January Van Geertruyden, Jean-Pierre Kaufmann, Stefan H.E |
| description | Summary Trained African giant pouched rats ( Cricetomys gambianus ) have potential for diagnosis of tuberculosis (TB). These rats target volatile compounds of Mycobacterium tuberculosis ( Mtb ) that cause TB. Mtb and nontuberculous mycobacteria (NTM) species are related to Nocardia and Rhodococcus spp., which are also acid-fast bacilli and can be misdiagnosed as Mtb in smear microscopy. Diagnostic performance of C. gambianus on in vitro -cultured mycobacterial and related pulmonary microbes is unknown. This study reports on the response of TB detection rats to cultures of reference Mtb , clinical Mtb , NTM, Nocardia ; Rhodococcus ; Streptomyces ; Bacillus ; and yeasts. Trained rats significantly discriminated Mtb from other microbes ( p |
| doi_str_mv | 10.1016/j.tube.2011.11.008 |
| format | Article |
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These rats target volatile compounds of Mycobacterium tuberculosis ( Mtb ) that cause TB. Mtb and nontuberculous mycobacteria (NTM) species are related to Nocardia and Rhodococcus spp., which are also acid-fast bacilli and can be misdiagnosed as Mtb in smear microscopy. Diagnostic performance of C. gambianus on in vitro -cultured mycobacterial and related pulmonary microbes is unknown. This study reports on the response of TB detection rats to cultures of reference Mtb , clinical Mtb , NTM, Nocardia ; Rhodococcus ; Streptomyces ; Bacillus ; and yeasts. Trained rats significantly discriminated Mtb from other microbes ( p < 0.008, Fisher’s exact test). Detection of Mtb cultures was age-related, with exponential and early stationary phase detected more frequently than early log phase and late stationary phase ( p < 0.001, Fisher’s test) (sensitivity = 83.33%, specificity = 94.4%, accuracy = 94%). The detection of naturally TB-infected sputum exceeded that of negative sputum mixed with Mtb , indicating that C. gambianus are conditioned to detect odours of TB-positive sputum better than spiked sputum. Although further studies on volatiles from detectable growth phases of Mtb are vital for identification of Mtb -specific volatiles detected by rats, our study underline the potential of C. gambianus for TB diagnosis.</description><identifier>ISSN: 1472-9792</identifier><identifier>EISSN: 1873-281X</identifier><identifier>DOI: 10.1016/j.tube.2011.11.008</identifier><identifier>PMID: 22197664</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Age ; Animals ; Bacilli ; Bacillus ; Bacterial Typing Techniques - methods ; Behavior, Animal - physiology ; Diagnosis, Differential ; Growth phase ; Humans ; Infectious Disease ; Lung ; Microorganisms ; Microscopy ; Mycobacterium smegmatis - classification ; Mycobacterium smegmatis - growth & development ; Mycobacterium smegmatis - metabolism ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - classification ; Mycobacterium tuberculosis - growth & development ; Mycobacterium tuberculosis - metabolism ; Nocardia ; Nontuberculous mycobacteria ; Odor ; Odorants - analysis ; Olfactory Perception - physiology ; Pulmonary/Respiratory ; Rhodococcus ; Rodentia - physiology ; Sensitivity and Specificity ; Sputum ; Sputum - microbiology ; stationary phase ; Streptomyces ; Tuberculosis ; Tuberculosis - diagnosis ; Volatile compounds ; Volatiles ; Volatilization</subject><ispartof>Tuberculosis (Edinburgh, Scotland), 2012-03, Vol.92 (2), p.182-186</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-9a39018e1ce1f46063b160ef0c3426af838f5eecae7f7cdfe0108465f6bb62763</citedby><cites>FETCH-LOGICAL-c487t-9a39018e1ce1f46063b160ef0c3426af838f5eecae7f7cdfe0108465f6bb62763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tube.2011.11.008$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22197664$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mgode, Georgies F</creatorcontrib><creatorcontrib>Weetjens, Bart J</creatorcontrib><creatorcontrib>Cox, Christophe</creatorcontrib><creatorcontrib>Jubitana, Maureen</creatorcontrib><creatorcontrib>Machang’u, Robert S</creatorcontrib><creatorcontrib>Lazar, Doris</creatorcontrib><creatorcontrib>Weiner, January</creatorcontrib><creatorcontrib>Van Geertruyden, Jean-Pierre</creatorcontrib><creatorcontrib>Kaufmann, Stefan H.E</creatorcontrib><title>Ability of Cricetomys rats to detect Mycobacterium tuberculosis and discriminate it from other microorganisms</title><title>Tuberculosis (Edinburgh, Scotland)</title><addtitle>Tuberculosis (Edinb)</addtitle><description>Summary Trained African giant pouched rats ( Cricetomys gambianus ) have potential for diagnosis of tuberculosis (TB). These rats target volatile compounds of Mycobacterium tuberculosis ( Mtb ) that cause TB. Mtb and nontuberculous mycobacteria (NTM) species are related to Nocardia and Rhodococcus spp., which are also acid-fast bacilli and can be misdiagnosed as Mtb in smear microscopy. Diagnostic performance of C. gambianus on in vitro -cultured mycobacterial and related pulmonary microbes is unknown. This study reports on the response of TB detection rats to cultures of reference Mtb , clinical Mtb , NTM, Nocardia ; Rhodococcus ; Streptomyces ; Bacillus ; and yeasts. Trained rats significantly discriminated Mtb from other microbes ( p < 0.008, Fisher’s exact test). Detection of Mtb cultures was age-related, with exponential and early stationary phase detected more frequently than early log phase and late stationary phase ( p < 0.001, Fisher’s test) (sensitivity = 83.33%, specificity = 94.4%, accuracy = 94%). The detection of naturally TB-infected sputum exceeded that of negative sputum mixed with Mtb , indicating that C. gambianus are conditioned to detect odours of TB-positive sputum better than spiked sputum. Although further studies on volatiles from detectable growth phases of Mtb are vital for identification of Mtb -specific volatiles detected by rats, our study underline the potential of C. gambianus for TB diagnosis.</description><subject>Age</subject><subject>Animals</subject><subject>Bacilli</subject><subject>Bacillus</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Behavior, Animal - physiology</subject><subject>Diagnosis, Differential</subject><subject>Growth phase</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Lung</subject><subject>Microorganisms</subject><subject>Microscopy</subject><subject>Mycobacterium smegmatis - classification</subject><subject>Mycobacterium smegmatis - growth & development</subject><subject>Mycobacterium smegmatis - metabolism</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - classification</subject><subject>Mycobacterium tuberculosis - growth & development</subject><subject>Mycobacterium tuberculosis - metabolism</subject><subject>Nocardia</subject><subject>Nontuberculous mycobacteria</subject><subject>Odor</subject><subject>Odorants - analysis</subject><subject>Olfactory Perception - physiology</subject><subject>Pulmonary/Respiratory</subject><subject>Rhodococcus</subject><subject>Rodentia - physiology</subject><subject>Sensitivity and Specificity</subject><subject>Sputum</subject><subject>Sputum - microbiology</subject><subject>stationary phase</subject><subject>Streptomyces</subject><subject>Tuberculosis</subject><subject>Tuberculosis - diagnosis</subject><subject>Volatile compounds</subject><subject>Volatiles</subject><subject>Volatilization</subject><issn>1472-9792</issn><issn>1873-281X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk2LFDEQhoMo7of-AQ-Sm156zEdPkgYRlkFXYcWDCt5COl3RjN2dNUkL_e-tZlYPHhYKKofnrVS9VYQ842zHGVevjru69LATjPMdBmPmATnnRstGGP7tIb5bLZpOd-KMXJRyZChihj0mZ0LwTivVnpPpqo9jrCtNgR5y9FDTtBaaXS20JjpABV_px9Wn3vkKOS4T3X7NfhlTiYW6eaBDLD7HKc6uAo2VhpwmmuoPyHSKPqeUv7s5lqk8IY-CGws8vcuX5Ou7t18O75ubT9cfDlc3jW-Nrk3nZMe4Ae6Bh1YxJXvsHALzshXKBSNN2AN4BzpoPwRgnJlW7YPqeyW0kpfkxanubU6_FijVTtgijKObIS3FdkLutZZKI_nyXhKN5oK3XWcQFScUJyolQ7C3OLTLK0Ibp-zRbtbYbSEWAxeCoud39Zd-guGf5O8GEHh9AgD9-B0h2-IjzB6GmNF6O6R4f_03_8n9GOfo3fgTVijHtOQZnbbcFmGZ_bydxHYRnDOGLRj5B-qEsz8</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Mgode, Georgies F</creator><creator>Weetjens, Bart J</creator><creator>Cox, Christophe</creator><creator>Jubitana, Maureen</creator><creator>Machang’u, Robert S</creator><creator>Lazar, Doris</creator><creator>Weiner, January</creator><creator>Van Geertruyden, Jean-Pierre</creator><creator>Kaufmann, Stefan H.E</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Ability of Cricetomys rats to detect Mycobacterium tuberculosis and discriminate it from other microorganisms</title><author>Mgode, Georgies F ; Weetjens, Bart J ; Cox, Christophe ; Jubitana, Maureen ; Machang’u, Robert S ; Lazar, Doris ; Weiner, January ; Van Geertruyden, Jean-Pierre ; Kaufmann, Stefan H.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-9a39018e1ce1f46063b160ef0c3426af838f5eecae7f7cdfe0108465f6bb62763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Age</topic><topic>Animals</topic><topic>Bacilli</topic><topic>Bacillus</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Behavior, Animal - physiology</topic><topic>Diagnosis, Differential</topic><topic>Growth phase</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Lung</topic><topic>Microorganisms</topic><topic>Microscopy</topic><topic>Mycobacterium smegmatis - classification</topic><topic>Mycobacterium smegmatis - growth & development</topic><topic>Mycobacterium smegmatis - metabolism</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - classification</topic><topic>Mycobacterium tuberculosis - growth & development</topic><topic>Mycobacterium tuberculosis - metabolism</topic><topic>Nocardia</topic><topic>Nontuberculous mycobacteria</topic><topic>Odor</topic><topic>Odorants - analysis</topic><topic>Olfactory Perception - physiology</topic><topic>Pulmonary/Respiratory</topic><topic>Rhodococcus</topic><topic>Rodentia - physiology</topic><topic>Sensitivity and Specificity</topic><topic>Sputum</topic><topic>Sputum - microbiology</topic><topic>stationary phase</topic><topic>Streptomyces</topic><topic>Tuberculosis</topic><topic>Tuberculosis - diagnosis</topic><topic>Volatile compounds</topic><topic>Volatiles</topic><topic>Volatilization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mgode, Georgies F</creatorcontrib><creatorcontrib>Weetjens, Bart J</creatorcontrib><creatorcontrib>Cox, Christophe</creatorcontrib><creatorcontrib>Jubitana, Maureen</creatorcontrib><creatorcontrib>Machang’u, Robert S</creatorcontrib><creatorcontrib>Lazar, Doris</creatorcontrib><creatorcontrib>Weiner, January</creatorcontrib><creatorcontrib>Van Geertruyden, Jean-Pierre</creatorcontrib><creatorcontrib>Kaufmann, Stefan H.E</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mgode, Georgies F</au><au>Weetjens, Bart J</au><au>Cox, Christophe</au><au>Jubitana, Maureen</au><au>Machang’u, Robert S</au><au>Lazar, Doris</au><au>Weiner, January</au><au>Van Geertruyden, Jean-Pierre</au><au>Kaufmann, Stefan H.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ability of Cricetomys rats to detect Mycobacterium tuberculosis and discriminate it from other microorganisms</atitle><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle><addtitle>Tuberculosis (Edinb)</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>92</volume><issue>2</issue><spage>182</spage><epage>186</epage><pages>182-186</pages><issn>1472-9792</issn><eissn>1873-281X</eissn><abstract>Summary Trained African giant pouched rats ( Cricetomys gambianus ) have potential for diagnosis of tuberculosis (TB). These rats target volatile compounds of Mycobacterium tuberculosis ( Mtb ) that cause TB. Mtb and nontuberculous mycobacteria (NTM) species are related to Nocardia and Rhodococcus spp., which are also acid-fast bacilli and can be misdiagnosed as Mtb in smear microscopy. Diagnostic performance of C. gambianus on in vitro -cultured mycobacterial and related pulmonary microbes is unknown. This study reports on the response of TB detection rats to cultures of reference Mtb , clinical Mtb , NTM, Nocardia ; Rhodococcus ; Streptomyces ; Bacillus ; and yeasts. Trained rats significantly discriminated Mtb from other microbes ( p < 0.008, Fisher’s exact test). Detection of Mtb cultures was age-related, with exponential and early stationary phase detected more frequently than early log phase and late stationary phase ( p < 0.001, Fisher’s test) (sensitivity = 83.33%, specificity = 94.4%, accuracy = 94%). The detection of naturally TB-infected sputum exceeded that of negative sputum mixed with Mtb , indicating that C. gambianus are conditioned to detect odours of TB-positive sputum better than spiked sputum. Although further studies on volatiles from detectable growth phases of Mtb are vital for identification of Mtb -specific volatiles detected by rats, our study underline the potential of C. gambianus for TB diagnosis.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>22197664</pmid><doi>10.1016/j.tube.2011.11.008</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Age Animals Bacilli Bacillus Bacterial Typing Techniques - methods Behavior, Animal - physiology Diagnosis, Differential Growth phase Humans Infectious Disease Lung Microorganisms Microscopy Mycobacterium smegmatis - classification Mycobacterium smegmatis - growth & development Mycobacterium smegmatis - metabolism Mycobacterium tuberculosis Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - growth & development Mycobacterium tuberculosis - metabolism Nocardia Nontuberculous mycobacteria Odor Odorants - analysis Olfactory Perception - physiology Pulmonary/Respiratory Rhodococcus Rodentia - physiology Sensitivity and Specificity Sputum Sputum - microbiology stationary phase Streptomyces Tuberculosis Tuberculosis - diagnosis Volatile compounds Volatiles Volatilization |
| title | Ability of Cricetomys rats to detect Mycobacterium tuberculosis and discriminate it from other microorganisms |
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