Long-term culture of functional hepatocytes on chemically modified collagen gels

For long-term maintenance of functional hepatocytes in primary culture, a new culture system with chemically modified type-I collagen gel was developed. Isolated hepatocytes spread as flat cells and rapidly lost their viability and functions when cultured on native collagen gel. In contrast, they su...

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Veröffentlicht in:	Cytotechnology (Dordrecht) 1996, Vol.21 (1), p.31-43
Hauptverfasser:	SHIMBARA, N, ATAWA, R, TAKASHINA, M, TANAKA, K, ICHIHARA, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Biological and medical sciences Biotechnology Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies
Online-Zugang:	Volltext
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