Novel and sensitive ELISA for the rapid quantification of recombinant p64K protein

The antigenic P64k protein from the pathogenic bacterium Neisseria meningitidis has been used as an immunological carrier in several conjugated vaccines. The aim of this report was to develop and validate a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for the quantification of recomb...

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Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 2011-06, Vol.55 (3), p.403-408
Hauptverfasser:	Leyva, Alberto, Sánchez, Julio C., Álvarez, Denis, Pérez, Bárbara, López, Lissette, Paz, Shaily, Torres, Edel, González, Tatiana, Font, Milagros, Hernández, Neyda, Valdés, Rodolfo
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis analysis of variance Analytical, structural and metabolic biochemistry Antigens, Bacterial - analysis Antigens, Bacterial - genetics bacteria Bacterial Outer Membrane Proteins - analysis Bacterial Outer Membrane Proteins - genetics Bacterial Vaccines Biological and medical sciences Conferences Conjugated vaccine Electrophoresis, Polyacrylamide Gel ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Escherichia coli - genetics Fundamental and applied biological sciences. Psychology General pharmacology guidelines Limit of Detection manufacturing Manufacturing process Medical sciences Neisseria meningitidis Neisseria meningitidis - immunology p64k-r Pharmacology. Drug treatments Protein Stability Recombinant Proteins - analysis Recombinant Proteins - genetics Reproducibility of Results Vaccines Validation
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