A genetically encoded Foerster resonance energy transfer biosensor for two-photon excitation microscopy

Pippi (phosphatidyl inositol phosphate indicator) is a biosensor based on the principle of FRET (Foerster resonance energy transfer), which consists of a pair of fluorescent proteins, CFP (cyan fluorescent protein) and YFP (yellow fluorescent protein), the PH domain sandwiched between them, and K-Ra...

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Veröffentlicht in:Analytical biochemistry 2011-06, Vol.413 (2), p.192-199
Hauptverfasser: Kumagai, Yuka, Kamioka, Yuji, Yagi, Shunsuke, Matsuda, Michiyuki, Kiyokawa, Etsuko
Format: Artikel
Sprache:eng
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