High Levels of Bioplastic Are Produced in Fertile Transplastomic Tobacco Plants Engineered with a Synthetic Operon for the Production of Polyhydroxybutyrate

An optimized genetic construct for plastid transformation of tobacco (Nicotiana tabacum) for the production of the renewable, biodegradable plastic polyhydroxybutyrate (PHB) was designed using an operon extension strategy. Bacterial genes encoding the PHB pathway enzymes were selected for use in thi...

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Veröffentlicht in:	Plant physiology (Bethesda) 2011-04, Vol.155 (4), p.1690-1708
Hauptverfasser:	Bohmert-Tatarev, Karen, McAvoy, Susan, Daughtry, Sean, Peoples, Oliver P, Snell, Kristi D
Format:	Artikel
Sprache:	eng
Schlagworte:	3' untranslated regions Acinetobacter Biodegradability biomass DNA DNA, Plant - genetics Enzymes FOCUS ISSUE ON PLASTID BIOLOGY gene expression Gene Expression Regulation, Plant Genetic Engineering - methods genetic markers Genetic Vectors host plants Hydroxybutyrates - metabolism Leaves Microscopy, Electron, Transmission Nicotiana - genetics Nicotiana - metabolism Nicotiana tabacum Operon Plant Leaves - metabolism Plant Leaves - ultrastructure Plants Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Plasmids Plastics - metabolism Plastids Plastids - genetics polyhydroxybutyrate Polymers recombinant proteins tobacco Transformation, Genetic Transgenes transgenic plants
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