Goat and sheep ovarian tissue cryopreservation: Effects on the morphology and development of primordial follicles and density of stromal cell
The effect of exposure to cryoprotectant and cryopreservation of goat and sheep ovarian cortical fragments on the morphology of primordial follicles, stromal cell density and follicular development was performed. Goat and sheep ovarian fragments were exposed to 1.0 or 1.5 M ethylene glycol (EG) for...
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| Veröffentlicht in: | Animal reproduction science 2010-10, Vol.122 (1), p.90-97 |
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| creator | Faustino, L.R. Santos, R.R. Silva, C.M.G. Pinto, L.C. Celestino, J.J.H. Campello, C.C. Figueiredo, J.R. Rodrigues, A.P.R. |
| description | The effect of exposure to cryoprotectant and cryopreservation of goat and sheep ovarian cortical fragments on the morphology of primordial follicles, stromal cell density and follicular development was performed. Goat and sheep ovarian fragments were exposed to 1.0 or 1.5
M ethylene glycol (EG) for 5, 10 or 20
min, followed or not by conventional cryopreservation. Follicular morphology and stromal cell density were evaluated by means of classical histological analysis. In addition, ovarian fragments were cultured for 1 or 7 days after cryopreservation to evaluate follicular development. Both exposure to cryoprotectant and cryopreservation of goat and sheep ovarian tissue did affect the morphology of primordial follicles and stromal cell density, except when goat ovarian tissue was exposed to EG for 5
min. Although exposure time did not influence follicular morphology in both species, increase in the exposure time from 5 to 20
min did reduce goat stromal cell density. Increase in EG concentration from 1.0 to 1.5
M did result in the decrease of the percentage of goat morphologically normal primordial follicles evaluated after exposure only.
In vitro culture of frozen–thawed goat and sheep ovarian tissue showed that exposure to 1.0
M, for 10
min, before freezing of goat and sheep ovarian tissue does not impair follicular developmental capacity. In addition, stromal cell density may play a role in follicular survival and development after cryopreservation of ovarian tissue. |
| doi_str_mv | 10.1016/j.anireprosci.2010.08.001 |
| format | Article |
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M ethylene glycol (EG) for 5, 10 or 20
min, followed or not by conventional cryopreservation. Follicular morphology and stromal cell density were evaluated by means of classical histological analysis. In addition, ovarian fragments were cultured for 1 or 7 days after cryopreservation to evaluate follicular development. Both exposure to cryoprotectant and cryopreservation of goat and sheep ovarian tissue did affect the morphology of primordial follicles and stromal cell density, except when goat ovarian tissue was exposed to EG for 5
min. Although exposure time did not influence follicular morphology in both species, increase in the exposure time from 5 to 20
min did reduce goat stromal cell density. Increase in EG concentration from 1.0 to 1.5
M did result in the decrease of the percentage of goat morphologically normal primordial follicles evaluated after exposure only.
In vitro culture of frozen–thawed goat and sheep ovarian tissue showed that exposure to 1.0
M, for 10
min, before freezing of goat and sheep ovarian tissue does not impair follicular developmental capacity. In addition, stromal cell density may play a role in follicular survival and development after cryopreservation of ovarian tissue.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2010.08.001</identifier><identifier>PMID: 20800393</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>adverse effects ; Animals ; Cell Count ; cell density ; Cell Survival - drug effects ; Cryopreservation ; Cryopreservation - veterinary ; cryoprotectants ; Cryoprotective Agents - pharmacology ; Development ; ethylene glycol ; Ethylene Glycol - pharmacology ; ewes ; Female ; females ; follicular development ; freeze-thaw cycles ; Goat ; Goats ; Organ Preservation - veterinary ; Ovarian Follicle - cytology ; Ovarian Follicle - drug effects ; Ovarian Follicle - growth & development ; ovarian follicles ; Ovary ; Primordial follicles ; Sheep ; species differences ; Stroma density ; stromal cells ; Stromal Cells - cytology ; tissue culture</subject><ispartof>Animal reproduction science, 2010-10, Vol.122 (1), p.90-97</ispartof><rights>2010 Elsevier B.V.</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-74bba3bc1c45696015772bb7a231857dedc95ae8030dbf3387a17d74056884e43</citedby><cites>FETCH-LOGICAL-c432t-74bba3bc1c45696015772bb7a231857dedc95ae8030dbf3387a17d74056884e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anireprosci.2010.08.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20800393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Faustino, L.R.</creatorcontrib><creatorcontrib>Santos, R.R.</creatorcontrib><creatorcontrib>Silva, C.M.G.</creatorcontrib><creatorcontrib>Pinto, L.C.</creatorcontrib><creatorcontrib>Celestino, J.J.H.</creatorcontrib><creatorcontrib>Campello, C.C.</creatorcontrib><creatorcontrib>Figueiredo, J.R.</creatorcontrib><creatorcontrib>Rodrigues, A.P.R.</creatorcontrib><title>Goat and sheep ovarian tissue cryopreservation: Effects on the morphology and development of primordial follicles and density of stromal cell</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The effect of exposure to cryoprotectant and cryopreservation of goat and sheep ovarian cortical fragments on the morphology of primordial follicles, stromal cell density and follicular development was performed. Goat and sheep ovarian fragments were exposed to 1.0 or 1.5
M ethylene glycol (EG) for 5, 10 or 20
min, followed or not by conventional cryopreservation. Follicular morphology and stromal cell density were evaluated by means of classical histological analysis. In addition, ovarian fragments were cultured for 1 or 7 days after cryopreservation to evaluate follicular development. Both exposure to cryoprotectant and cryopreservation of goat and sheep ovarian tissue did affect the morphology of primordial follicles and stromal cell density, except when goat ovarian tissue was exposed to EG for 5
min. Although exposure time did not influence follicular morphology in both species, increase in the exposure time from 5 to 20
min did reduce goat stromal cell density. Increase in EG concentration from 1.0 to 1.5
M did result in the decrease of the percentage of goat morphologically normal primordial follicles evaluated after exposure only.
In vitro culture of frozen–thawed goat and sheep ovarian tissue showed that exposure to 1.0
M, for 10
min, before freezing of goat and sheep ovarian tissue does not impair follicular developmental capacity. In addition, stromal cell density may play a role in follicular survival and development after cryopreservation of ovarian tissue.</description><subject>adverse effects</subject><subject>Animals</subject><subject>Cell Count</subject><subject>cell density</subject><subject>Cell Survival - drug effects</subject><subject>Cryopreservation</subject><subject>Cryopreservation - veterinary</subject><subject>cryoprotectants</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Development</subject><subject>ethylene glycol</subject><subject>Ethylene Glycol - pharmacology</subject><subject>ewes</subject><subject>Female</subject><subject>females</subject><subject>follicular development</subject><subject>freeze-thaw cycles</subject><subject>Goat</subject><subject>Goats</subject><subject>Organ Preservation - veterinary</subject><subject>Ovarian Follicle - cytology</subject><subject>Ovarian Follicle - drug effects</subject><subject>Ovarian Follicle - growth & development</subject><subject>ovarian follicles</subject><subject>Ovary</subject><subject>Primordial follicles</subject><subject>Sheep</subject><subject>species differences</subject><subject>Stroma density</subject><subject>stromal cells</subject><subject>Stromal Cells - cytology</subject><subject>tissue culture</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2O0zAURi0EYsrAK4BZsUq5tpPYYYeqYUAaiQXM2nLsm6mrNA62W6kPwTuPQwtiBytL1rnf_TmEvGWwZsDa97u1mXzEOYZk_ZpD-Qe1BmBPyIopKSrOBX9KViCkqmrB4Yq8SGkHALJtu-fkioMCEJ1YkZ-3wWRqJkfTFnGm4WiiNxPNPqUDUhtPYY6YMB5N9mH6QG-GAW1ONBRmi3Qf4rwNY3g4_QpxeMQxzHucMg0DnaMvgPNmpEMYR29HTBduSj6fFiblGPYFsDiOL8mzwYwJX13ea3L_6eb75nN19_X2y-bjXWXLNrmSdd8b0Vtm66btWmCNlLzvpeGCqUY6dLZrDCoQ4PpBCCUNk07W0LRK1ViLa_LunFtO-OOAKeu9T8sAZsJwSLorQZ3krfwnKRtZN51ivJDdmbRFS4o46GV7E0-agV606Z3-S5tetGlQumgrta8vXQ79Ht2fyt-eCvDmDAwmaPMQfdL330qCAKY6Ba0qxOZMYLnb0WPUpQlOFl1pabN2wf_HII9UJbrv</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Faustino, L.R.</creator><creator>Santos, R.R.</creator><creator>Silva, C.M.G.</creator><creator>Pinto, L.C.</creator><creator>Celestino, J.J.H.</creator><creator>Campello, C.C.</creator><creator>Figueiredo, J.R.</creator><creator>Rodrigues, A.P.R.</creator><general>Elsevier B.V</general><general>[Amsterdam]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20101001</creationdate><title>Goat and sheep ovarian tissue cryopreservation: Effects on the morphology and development of primordial follicles and density of stromal cell</title><author>Faustino, L.R. ; Santos, R.R. ; Silva, C.M.G. ; Pinto, L.C. ; Celestino, J.J.H. ; Campello, C.C. ; Figueiredo, J.R. ; Rodrigues, A.P.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-74bba3bc1c45696015772bb7a231857dedc95ae8030dbf3387a17d74056884e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>adverse effects</topic><topic>Animals</topic><topic>Cell Count</topic><topic>cell density</topic><topic>Cell Survival - drug effects</topic><topic>Cryopreservation</topic><topic>Cryopreservation - veterinary</topic><topic>cryoprotectants</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Development</topic><topic>ethylene glycol</topic><topic>Ethylene Glycol - pharmacology</topic><topic>ewes</topic><topic>Female</topic><topic>females</topic><topic>follicular development</topic><topic>freeze-thaw cycles</topic><topic>Goat</topic><topic>Goats</topic><topic>Organ Preservation - veterinary</topic><topic>Ovarian Follicle - cytology</topic><topic>Ovarian Follicle - drug effects</topic><topic>Ovarian Follicle - growth & development</topic><topic>ovarian follicles</topic><topic>Ovary</topic><topic>Primordial follicles</topic><topic>Sheep</topic><topic>species differences</topic><topic>Stroma density</topic><topic>stromal cells</topic><topic>Stromal Cells - cytology</topic><topic>tissue culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Faustino, L.R.</creatorcontrib><creatorcontrib>Santos, R.R.</creatorcontrib><creatorcontrib>Silva, C.M.G.</creatorcontrib><creatorcontrib>Pinto, L.C.</creatorcontrib><creatorcontrib>Celestino, J.J.H.</creatorcontrib><creatorcontrib>Campello, C.C.</creatorcontrib><creatorcontrib>Figueiredo, J.R.</creatorcontrib><creatorcontrib>Rodrigues, A.P.R.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Faustino, L.R.</au><au>Santos, R.R.</au><au>Silva, C.M.G.</au><au>Pinto, L.C.</au><au>Celestino, J.J.H.</au><au>Campello, C.C.</au><au>Figueiredo, J.R.</au><au>Rodrigues, A.P.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Goat and sheep ovarian tissue cryopreservation: Effects on the morphology and development of primordial follicles and density of stromal cell</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>122</volume><issue>1</issue><spage>90</spage><epage>97</epage><pages>90-97</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The effect of exposure to cryoprotectant and cryopreservation of goat and sheep ovarian cortical fragments on the morphology of primordial follicles, stromal cell density and follicular development was performed. Goat and sheep ovarian fragments were exposed to 1.0 or 1.5
M ethylene glycol (EG) for 5, 10 or 20
min, followed or not by conventional cryopreservation. Follicular morphology and stromal cell density were evaluated by means of classical histological analysis. In addition, ovarian fragments were cultured for 1 or 7 days after cryopreservation to evaluate follicular development. Both exposure to cryoprotectant and cryopreservation of goat and sheep ovarian tissue did affect the morphology of primordial follicles and stromal cell density, except when goat ovarian tissue was exposed to EG for 5
min. Although exposure time did not influence follicular morphology in both species, increase in the exposure time from 5 to 20
min did reduce goat stromal cell density. Increase in EG concentration from 1.0 to 1.5
M did result in the decrease of the percentage of goat morphologically normal primordial follicles evaluated after exposure only.
In vitro culture of frozen–thawed goat and sheep ovarian tissue showed that exposure to 1.0
M, for 10
min, before freezing of goat and sheep ovarian tissue does not impair follicular developmental capacity. In addition, stromal cell density may play a role in follicular survival and development after cryopreservation of ovarian tissue.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>20800393</pmid><doi>10.1016/j.anireprosci.2010.08.001</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0378-4320 |
| ispartof | Animal reproduction science, 2010-10, Vol.122 (1), p.90-97 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | adverse effects Animals Cell Count cell density Cell Survival - drug effects Cryopreservation Cryopreservation - veterinary cryoprotectants Cryoprotective Agents - pharmacology Development ethylene glycol Ethylene Glycol - pharmacology ewes Female females follicular development freeze-thaw cycles Goat Goats Organ Preservation - veterinary Ovarian Follicle - cytology Ovarian Follicle - drug effects Ovarian Follicle - growth & development ovarian follicles Ovary Primordial follicles Sheep species differences Stroma density stromal cells Stromal Cells - cytology tissue culture |
| title | Goat and sheep ovarian tissue cryopreservation: Effects on the morphology and development of primordial follicles and density of stromal cell |
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