Effects of organic solvents on activity and conformation of recombinant Candida antarctica lipase A produced by Pichia pastoris

► The catalytic activity of CALA increased after treatment with acetonitrile or acetone. ► The variance of catalytic activity mainly attributed to the secondary structure changes. ► The acetonitrile treatment could increase the thermal and pH stability of CALA. The effect of various organic solvents...

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Veröffentlicht in:	Process biochemistry (1991) 2012-03, Vol.47 (3), p.533-537
Hauptverfasser:	Yang, Chunfang, Wang, Fanghua, Lan, Dongming, Whiteley, Chris, Yang, Bo, Wang, Yonghua
Format:	Artikel
Sprache:	eng
Schlagworte:	acetone acetonitrile Candida antarctica Candida antarctica lipase A Catalytic activity esterification ethanol ethyl acetate Fourier transform infrared spectroscopy Olea olive oil Organic solvents Pichia pastoris Pseudozyma antarctica Secondary structure solvents variance
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