Catalytic properties of β-d-xylosidase XylBH43 from Bacillus halodurans C-125 and mutant XylBH43-W147G

Recombinant glycoside hydrolase family 43 member β-d-xylosidase XylBH43 from Bacillus halodurans C-125 was expressed in Escherichia coli with C-terminal His-tag. Compared to structurally homologous β-xylosidase SXA, another GH43 member and the most active xylosidase known to date, the kcat value for...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Process biochemistry (1991) 2012-03, Vol.47 (3), p.366-372
Hauptverfasser:	Wagschal, Kurt, Jordan, Douglas B., Braker, Jay D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus halodurans binding capacity Bioenergy biomass Escherichia coli genetic variation GH43 glucose glycosides hydrolysis Inhibition mutants Protein engineering saccharification thermal stability xylan 1,4-beta-xylosidase xylose β-d-Xylosidase
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!