Semi-automated quantitative image analysis of podocyte desmin immunoreactivity as a sensitive marker for acute glomerular damage in the rat puromycin aminonucleoside nephrosis (PAN) model
Glomerular visceral epithelial cells or podocytes are crucial for glomerular function and podocyte damage has been shown to be inevitably involved in glomerulopathies. Podocytes react to injury in a stereotypic manner. Accompanying morphologic changes is altered expression of intermediate filaments....
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| Veröffentlicht in: | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2012, Vol.64 (1), p.45-49 |
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| container_title | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie |
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| creator | Herrmann, Annika Tozzo, Effie Funk, Jürgen |
| description | Glomerular visceral epithelial cells or podocytes are crucial for glomerular function and podocyte damage has been shown to be inevitably involved in glomerulopathies. Podocytes react to injury in a stereotypic manner. Accompanying morphologic changes is altered expression of intermediate filaments. Desmin is strongly upregulated in injured podocytes. Here we show, that semi-automated quantitative image analysis of desmin immunoreactivity in glomerula is a valid and sensitive marker for acute podocyte and thus glomerular damage in the puromycin aminonucleoside nephrosis (PAN) model in the rat with the potential of an efficacy marker in animal disease models as well as a toxicity marker for podocyte injury. Additionally, a panel of acknowledged urinary kidney biomarkers was evaluated for utility in the PAN model. |
| doi_str_mv | 10.1016/j.etp.2010.06.004 |
| format | Article |
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Podocytes react to injury in a stereotypic manner. Accompanying morphologic changes is altered expression of intermediate filaments. Desmin is strongly upregulated in injured podocytes. Here we show, that semi-automated quantitative image analysis of desmin immunoreactivity in glomerula is a valid and sensitive marker for acute podocyte and thus glomerular damage in the puromycin aminonucleoside nephrosis (PAN) model in the rat with the potential of an efficacy marker in animal disease models as well as a toxicity marker for podocyte injury. Additionally, a panel of acknowledged urinary kidney biomarkers was evaluated for utility in the PAN model.</description><identifier>ISSN: 0940-2993</identifier><identifier>EISSN: 1618-1433</identifier><identifier>DOI: 10.1016/j.etp.2010.06.004</identifier><identifier>PMID: 20591637</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>Acute Disease ; animal disease models ; Animals ; Biological and medical sciences ; biomarkers ; Biomarkers - metabolism ; Biomarkers - urine ; Desmin ; Desmin - metabolism ; Disease Models, Animal ; Dose-Response Relationship, Drug ; epithelial cells ; Image analysis ; Image Interpretation, Computer-Assisted ; Immunohistochemistry ; intermediate filaments ; Investigative techniques, diagnostic techniques (general aspects) ; Kidney Function Tests ; kidneys ; Male ; Medical sciences ; nephrosis ; Nephrosis - chemically induced ; Nephrosis - metabolism ; Nephrosis - pathology ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Podocyte injury ; Podocytes - drug effects ; Podocytes - metabolism ; Podocytes - pathology ; puromycin ; Puromycin Aminonucleoside - toxicity ; Puromycin aminonucleoside nephrosis ; Rats ; Rats, Sprague-Dawley ; Severity of Illness Index ; toxicity ; Urinary biomarkers</subject><ispartof>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2012, Vol.64 (1), p.45-49</ispartof><rights>2010 Elsevier GmbH</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-2642880570532f0fa28fa5ff44cee27bb39810a858648b8fc6bd5e83abe5dd3f3</citedby><cites>FETCH-LOGICAL-c504t-2642880570532f0fa28fa5ff44cee27bb39810a858648b8fc6bd5e83abe5dd3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.etp.2010.06.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25416653$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20591637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herrmann, Annika</creatorcontrib><creatorcontrib>Tozzo, Effie</creatorcontrib><creatorcontrib>Funk, Jürgen</creatorcontrib><title>Semi-automated quantitative image analysis of podocyte desmin immunoreactivity as a sensitive marker for acute glomerular damage in the rat puromycin aminonucleoside nephrosis (PAN) model</title><title>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</title><addtitle>Exp Toxicol Pathol</addtitle><description>Glomerular visceral epithelial cells or podocytes are crucial for glomerular function and podocyte damage has been shown to be inevitably involved in glomerulopathies. Podocytes react to injury in a stereotypic manner. Accompanying morphologic changes is altered expression of intermediate filaments. Desmin is strongly upregulated in injured podocytes. Here we show, that semi-automated quantitative image analysis of desmin immunoreactivity in glomerula is a valid and sensitive marker for acute podocyte and thus glomerular damage in the puromycin aminonucleoside nephrosis (PAN) model in the rat with the potential of an efficacy marker in animal disease models as well as a toxicity marker for podocyte injury. Additionally, a panel of acknowledged urinary kidney biomarkers was evaluated for utility in the PAN model.</description><subject>Acute Disease</subject><subject>animal disease models</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>biomarkers</subject><subject>Biomarkers - metabolism</subject><subject>Biomarkers - urine</subject><subject>Desmin</subject><subject>Desmin - metabolism</subject><subject>Disease Models, Animal</subject><subject>Dose-Response Relationship, Drug</subject><subject>epithelial cells</subject><subject>Image analysis</subject><subject>Image Interpretation, Computer-Assisted</subject><subject>Immunohistochemistry</subject><subject>intermediate filaments</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kidney Function Tests</subject><subject>kidneys</subject><subject>Male</subject><subject>Medical sciences</subject><subject>nephrosis</subject><subject>Nephrosis - chemically induced</subject><subject>Nephrosis - metabolism</subject><subject>Nephrosis - pathology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Podocyte injury</subject><subject>Podocytes - drug effects</subject><subject>Podocytes - metabolism</subject><subject>Podocytes - pathology</subject><subject>puromycin</subject><subject>Puromycin Aminonucleoside - toxicity</subject><subject>Puromycin aminonucleoside nephrosis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Severity of Illness Index</subject><subject>toxicity</subject><subject>Urinary biomarkers</subject><issn>0940-2993</issn><issn>1618-1433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhSMEokPhAdiANwhYZPBPnCZiVVX8SRUgla6tG_t66iGOU9upNM_Gy-HpDLBjZdn6zrnH91TVc0bXjLL23XaNeV5zWu60XVPaPKhWrGVdzRohHlYr2je05n0vTqonKW0p5bSX7HF1wqnsWSvOVtWvK_SuhiUHDxkNuV1gyi5DdndInIcNEphg3CWXSLBkDiboXUZiMHk3FcIvU4gIughc3hFIBEjCKbl7Bw_xJ0ZiQySgl6LbjMFjXEaIxMC9fXHJN0giZDIvMfidLi9QzMO06BFDcgbJhPNNDPsQb76ff31LfDA4Pq0eWRgTPjuep9X1xw8_Lj7Xl98-fbk4v6y1pE2uedvwrqPyjErBLbXAOwvS2qbRiPxsGETfMQqd7NqmGzqr28FI7AQMKI0RVpxWrw--cwy3C6asvEsaxxEmDEtSPResL6NoIdmB1CVsimjVHMsS404xqvaVqa0qlal9ZYq2qlRWNC-O7svg0fxV_OmoAK-OACQNo40waZf-cbJhbStF4V4eOAtBwSYW5vqqTJK0DC5f54V4fyCwbOvOYVRJO5w0GhdRZ2WC-0_Q38lSwjc</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Herrmann, Annika</creator><creator>Tozzo, Effie</creator><creator>Funk, Jürgen</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>2012</creationdate><title>Semi-automated quantitative image analysis of podocyte desmin immunoreactivity as a sensitive marker for acute glomerular damage in the rat puromycin aminonucleoside nephrosis (PAN) model</title><author>Herrmann, Annika ; Tozzo, Effie ; Funk, Jürgen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-2642880570532f0fa28fa5ff44cee27bb39810a858648b8fc6bd5e83abe5dd3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acute Disease</topic><topic>animal disease models</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>biomarkers</topic><topic>Biomarkers - metabolism</topic><topic>Biomarkers - urine</topic><topic>Desmin</topic><topic>Desmin - metabolism</topic><topic>Disease Models, Animal</topic><topic>Dose-Response Relationship, Drug</topic><topic>epithelial cells</topic><topic>Image analysis</topic><topic>Image Interpretation, Computer-Assisted</topic><topic>Immunohistochemistry</topic><topic>intermediate filaments</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kidney Function Tests</topic><topic>kidneys</topic><topic>Male</topic><topic>Medical sciences</topic><topic>nephrosis</topic><topic>Nephrosis - chemically induced</topic><topic>Nephrosis - metabolism</topic><topic>Nephrosis - pathology</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Podocyte injury</topic><topic>Podocytes - drug effects</topic><topic>Podocytes - metabolism</topic><topic>Podocytes - pathology</topic><topic>puromycin</topic><topic>Puromycin Aminonucleoside - toxicity</topic><topic>Puromycin aminonucleoside nephrosis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Severity of Illness Index</topic><topic>toxicity</topic><topic>Urinary biomarkers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herrmann, Annika</creatorcontrib><creatorcontrib>Tozzo, Effie</creatorcontrib><creatorcontrib>Funk, Jürgen</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herrmann, Annika</au><au>Tozzo, Effie</au><au>Funk, Jürgen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Semi-automated quantitative image analysis of podocyte desmin immunoreactivity as a sensitive marker for acute glomerular damage in the rat puromycin aminonucleoside nephrosis (PAN) model</atitle><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle><addtitle>Exp Toxicol Pathol</addtitle><date>2012</date><risdate>2012</risdate><volume>64</volume><issue>1</issue><spage>45</spage><epage>49</epage><pages>45-49</pages><issn>0940-2993</issn><eissn>1618-1433</eissn><abstract>Glomerular visceral epithelial cells or podocytes are crucial for glomerular function and podocyte damage has been shown to be inevitably involved in glomerulopathies. Podocytes react to injury in a stereotypic manner. Accompanying morphologic changes is altered expression of intermediate filaments. Desmin is strongly upregulated in injured podocytes. Here we show, that semi-automated quantitative image analysis of desmin immunoreactivity in glomerula is a valid and sensitive marker for acute podocyte and thus glomerular damage in the puromycin aminonucleoside nephrosis (PAN) model in the rat with the potential of an efficacy marker in animal disease models as well as a toxicity marker for podocyte injury. Additionally, a panel of acknowledged urinary kidney biomarkers was evaluated for utility in the PAN model.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>20591637</pmid><doi>10.1016/j.etp.2010.06.004</doi><tpages>5</tpages></addata></record> |
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| ispartof | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2012, Vol.64 (1), p.45-49 |
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| subjects | Acute Disease animal disease models Animals Biological and medical sciences biomarkers Biomarkers - metabolism Biomarkers - urine Desmin Desmin - metabolism Disease Models, Animal Dose-Response Relationship, Drug epithelial cells Image analysis Image Interpretation, Computer-Assisted Immunohistochemistry intermediate filaments Investigative techniques, diagnostic techniques (general aspects) Kidney Function Tests kidneys Male Medical sciences nephrosis Nephrosis - chemically induced Nephrosis - metabolism Nephrosis - pathology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Podocyte injury Podocytes - drug effects Podocytes - metabolism Podocytes - pathology puromycin Puromycin Aminonucleoside - toxicity Puromycin aminonucleoside nephrosis Rats Rats, Sprague-Dawley Severity of Illness Index toxicity Urinary biomarkers |
| title | Semi-automated quantitative image analysis of podocyte desmin immunoreactivity as a sensitive marker for acute glomerular damage in the rat puromycin aminonucleoside nephrosis (PAN) model |
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