Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD–MS/MS
► A rapid and comprehensive UPLC-DAD–MS method was established to assess the quality of four dosage forms of XFZY. ► 25 compounds were identified by UPLC–MS/MS in four dosage forms, which originated from ten herbs of XFZY. ► 12 active compounds were quantified in four dosage forms of XFZY within 20m...
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| creator | Zhang, Lei Zhu, Lin Wang, Yuefei Jiang, Zhenzuo Chai, Xin Zhu, Yan Gao, Xiumei Qi, Aidi |
| description | ► A rapid and comprehensive UPLC-DAD–MS method was established to assess the quality of four dosage forms of XFZY. ► 25 compounds were identified by UPLC–MS/MS in four dosage forms, which originated from ten herbs of XFZY. ► 12 active compounds were quantified in four dosage forms of XFZY within 20min.
Xue Fu Zhu Yu (XFZY), a classic recipe in traditional Chinese medicine (TCM), has been demonstrated to show protective effects on cardiovascular system. For quality control of XFZY products, qualitative analysis using ultra high performance liquid chromatography with diode-array detector–tandem mass spectrometry (UPLC-DAD–MS) was undertaken. Twenty-eight compounds from XFZY were simultaneously detected; among them, seventeen compounds were unequivocally identified, and another eight compounds were tentatively characterized. According to qualitative results, a new method for quantitative analysis of XFZY has been established by ultra high performance liquid chromatography coupled with diode array detector (UPLC-DAD). Twelve representative compounds unequivocally identified were used as chemical markers in quantitative analysis, including 5-hydroxymethyl-2-furaldehyde (5-HMF), hydroxysafflor yellow A (HSYA), amygdalin, albiflorin, paeoniflorin, liquiritin, ferulic acid (FA), naringin, hesperidin, neohesperidin (NH), isoliquiritigenin (IL) and glycyrrhizic acid (GA), which were derived from nine of eleven herbs of XFZY except Platycodon grandiflorum (Jacq.) A. DC. (Jiegeng) and Bupleurum chinense DC. (Chaihu). This UPLC method was validated in terms of linearity, LOD and LOQ, precision, repeatability, stability, and recovery tests. Quality control of XFZY products in total fourteen samples by four dosage forms was examined by this method, and results confirmed its feasibility and reliability in practice. |
| doi_str_mv | 10.1016/j.jpba.2011.12.026 |
| format | Article |
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Xue Fu Zhu Yu (XFZY), a classic recipe in traditional Chinese medicine (TCM), has been demonstrated to show protective effects on cardiovascular system. For quality control of XFZY products, qualitative analysis using ultra high performance liquid chromatography with diode-array detector–tandem mass spectrometry (UPLC-DAD–MS) was undertaken. Twenty-eight compounds from XFZY were simultaneously detected; among them, seventeen compounds were unequivocally identified, and another eight compounds were tentatively characterized. According to qualitative results, a new method for quantitative analysis of XFZY has been established by ultra high performance liquid chromatography coupled with diode array detector (UPLC-DAD). Twelve representative compounds unequivocally identified were used as chemical markers in quantitative analysis, including 5-hydroxymethyl-2-furaldehyde (5-HMF), hydroxysafflor yellow A (HSYA), amygdalin, albiflorin, paeoniflorin, liquiritin, ferulic acid (FA), naringin, hesperidin, neohesperidin (NH), isoliquiritigenin (IL) and glycyrrhizic acid (GA), which were derived from nine of eleven herbs of XFZY except Platycodon grandiflorum (Jacq.) A. DC. (Jiegeng) and Bupleurum chinense DC. (Chaihu). This UPLC method was validated in terms of linearity, LOD and LOQ, precision, repeatability, stability, and recovery tests. Quality control of XFZY products in total fourteen samples by four dosage forms was examined by this method, and results confirmed its feasibility and reliability in practice.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2011.12.026</identifier><identifier>PMID: 22264846</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>amygdalin ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Bupleurum ; cardiovascular system ; Chromatography, Liquid - methods ; drug formulations ; Drugs, Chinese Herbal - chemistry ; ferulic acid ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; glycyrrhizin ; herbs ; hesperidin ; hydroxymethylfurfural ; mass spectrometry ; Medical sciences ; naringin ; Oriental traditional medicine ; Pharmacology. Drug treatments ; Platycodon grandiflorum ; protective effect ; Qualitative analysis ; quality control ; Quantitative analysis ; Spectrometry, Mass, Electrospray Ionization - methods ; Tandem Mass Spectrometry - methods ; ultra-performance liquid chromatography ; UPLC-DAD ; UPLC–MS ; Xue Fu Zhu Yu</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2012-03, Vol.62, p.203-209</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-be41b9f29c213521d89565769faab2b6002f2446bf7d05eec68d95732605e8713</citedby><cites>FETCH-LOGICAL-c409t-be41b9f29c213521d89565769faab2b6002f2446bf7d05eec68d95732605e8713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2011.12.026$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25703460$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22264846$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Zhu, Lin</creatorcontrib><creatorcontrib>Wang, Yuefei</creatorcontrib><creatorcontrib>Jiang, Zhenzuo</creatorcontrib><creatorcontrib>Chai, Xin</creatorcontrib><creatorcontrib>Zhu, Yan</creatorcontrib><creatorcontrib>Gao, Xiumei</creatorcontrib><creatorcontrib>Qi, Aidi</creatorcontrib><title>Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD–MS/MS</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>► A rapid and comprehensive UPLC-DAD–MS method was established to assess the quality of four dosage forms of XFZY. ► 25 compounds were identified by UPLC–MS/MS in four dosage forms, which originated from ten herbs of XFZY. ► 12 active compounds were quantified in four dosage forms of XFZY within 20min.
Xue Fu Zhu Yu (XFZY), a classic recipe in traditional Chinese medicine (TCM), has been demonstrated to show protective effects on cardiovascular system. For quality control of XFZY products, qualitative analysis using ultra high performance liquid chromatography with diode-array detector–tandem mass spectrometry (UPLC-DAD–MS) was undertaken. Twenty-eight compounds from XFZY were simultaneously detected; among them, seventeen compounds were unequivocally identified, and another eight compounds were tentatively characterized. According to qualitative results, a new method for quantitative analysis of XFZY has been established by ultra high performance liquid chromatography coupled with diode array detector (UPLC-DAD). Twelve representative compounds unequivocally identified were used as chemical markers in quantitative analysis, including 5-hydroxymethyl-2-furaldehyde (5-HMF), hydroxysafflor yellow A (HSYA), amygdalin, albiflorin, paeoniflorin, liquiritin, ferulic acid (FA), naringin, hesperidin, neohesperidin (NH), isoliquiritigenin (IL) and glycyrrhizic acid (GA), which were derived from nine of eleven herbs of XFZY except Platycodon grandiflorum (Jacq.) A. DC. (Jiegeng) and Bupleurum chinense DC. (Chaihu). This UPLC method was validated in terms of linearity, LOD and LOQ, precision, repeatability, stability, and recovery tests. Quality control of XFZY products in total fourteen samples by four dosage forms was examined by this method, and results confirmed its feasibility and reliability in practice.</description><subject>amygdalin</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Bupleurum</subject><subject>cardiovascular system</subject><subject>Chromatography, Liquid - methods</subject><subject>drug formulations</subject><subject>Drugs, Chinese Herbal - chemistry</subject><subject>ferulic acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>glycyrrhizin</subject><subject>herbs</subject><subject>hesperidin</subject><subject>hydroxymethylfurfural</subject><subject>mass spectrometry</subject><subject>Medical sciences</subject><subject>naringin</subject><subject>Oriental traditional medicine</subject><subject>Pharmacology. Drug treatments</subject><subject>Platycodon grandiflorum</subject><subject>protective effect</subject><subject>Qualitative analysis</subject><subject>quality control</subject><subject>Quantitative analysis</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>ultra-performance liquid chromatography</subject><subject>UPLC-DAD</subject><subject>UPLC–MS</subject><subject>Xue Fu Zhu Yu</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAQgC0EokvhBThALohT0rHj2LHEpdpSqLRVkZaVCgcsx7Gpo91kaztI5cQ78IZ9kjrKAjdOoxl98_ch9BJDgQGzk67o9o0qCGBcYFIAYY_QAte8zAmj14_RAniJcw51dYSehdABQIUFfYqOCElETdkCfVveKK90NN79VNENfab6NrsdVR-ddXouDTbbqW7wmR76EF0cTR_DVL0eTXY-Zl9vxuzLmDV32ebTapmfnZ7d__p9uT65XD9HT6zaBvPiEI_R5vz95-XHfHX14WJ5uso1BRHzxlDcCEuEJrisCG5rUbGKM2GVakjDAIgllLLG8hYqYzSrW1HxkrCU1RyXx-jtPHfvh9vRhCh3Lmiz3areDGOQghDOSkx5IslMaj-E4I2Ve-92yt9JDHLSKjs5aZWTVomJTFpT06vD-LHZmfZvyx-PCXhzAFTQamu96rUL_7iKQ0kZJO71zFk1SPXdJ2azTpsqAExA0OmTdzNhkq4fzngZtDO9Nq3zRkfZDu5_lz4ArCWd7Q</recordid><startdate>20120325</startdate><enddate>20120325</enddate><creator>Zhang, Lei</creator><creator>Zhu, Lin</creator><creator>Wang, Yuefei</creator><creator>Jiang, Zhenzuo</creator><creator>Chai, Xin</creator><creator>Zhu, Yan</creator><creator>Gao, Xiumei</creator><creator>Qi, Aidi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120325</creationdate><title>Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD–MS/MS</title><author>Zhang, Lei ; Zhu, Lin ; Wang, Yuefei ; Jiang, Zhenzuo ; Chai, Xin ; Zhu, Yan ; Gao, Xiumei ; Qi, Aidi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-be41b9f29c213521d89565769faab2b6002f2446bf7d05eec68d95732605e8713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>amygdalin</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Bupleurum</topic><topic>cardiovascular system</topic><topic>Chromatography, Liquid - methods</topic><topic>drug formulations</topic><topic>Drugs, Chinese Herbal - chemistry</topic><topic>ferulic acid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>glycyrrhizin</topic><topic>herbs</topic><topic>hesperidin</topic><topic>hydroxymethylfurfural</topic><topic>mass spectrometry</topic><topic>Medical sciences</topic><topic>naringin</topic><topic>Oriental traditional medicine</topic><topic>Pharmacology. Drug treatments</topic><topic>Platycodon grandiflorum</topic><topic>protective effect</topic><topic>Qualitative analysis</topic><topic>quality control</topic><topic>Quantitative analysis</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>ultra-performance liquid chromatography</topic><topic>UPLC-DAD</topic><topic>UPLC–MS</topic><topic>Xue Fu Zhu Yu</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Zhu, Lin</creatorcontrib><creatorcontrib>Wang, Yuefei</creatorcontrib><creatorcontrib>Jiang, Zhenzuo</creatorcontrib><creatorcontrib>Chai, Xin</creatorcontrib><creatorcontrib>Zhu, Yan</creatorcontrib><creatorcontrib>Gao, Xiumei</creatorcontrib><creatorcontrib>Qi, Aidi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Lei</au><au>Zhu, Lin</au><au>Wang, Yuefei</au><au>Jiang, Zhenzuo</au><au>Chai, Xin</au><au>Zhu, Yan</au><au>Gao, Xiumei</au><au>Qi, Aidi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD–MS/MS</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2012-03-25</date><risdate>2012</risdate><volume>62</volume><spage>203</spage><epage>209</epage><pages>203-209</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>► A rapid and comprehensive UPLC-DAD–MS method was established to assess the quality of four dosage forms of XFZY. ► 25 compounds were identified by UPLC–MS/MS in four dosage forms, which originated from ten herbs of XFZY. ► 12 active compounds were quantified in four dosage forms of XFZY within 20min.
Xue Fu Zhu Yu (XFZY), a classic recipe in traditional Chinese medicine (TCM), has been demonstrated to show protective effects on cardiovascular system. For quality control of XFZY products, qualitative analysis using ultra high performance liquid chromatography with diode-array detector–tandem mass spectrometry (UPLC-DAD–MS) was undertaken. Twenty-eight compounds from XFZY were simultaneously detected; among them, seventeen compounds were unequivocally identified, and another eight compounds were tentatively characterized. According to qualitative results, a new method for quantitative analysis of XFZY has been established by ultra high performance liquid chromatography coupled with diode array detector (UPLC-DAD). Twelve representative compounds unequivocally identified were used as chemical markers in quantitative analysis, including 5-hydroxymethyl-2-furaldehyde (5-HMF), hydroxysafflor yellow A (HSYA), amygdalin, albiflorin, paeoniflorin, liquiritin, ferulic acid (FA), naringin, hesperidin, neohesperidin (NH), isoliquiritigenin (IL) and glycyrrhizic acid (GA), which were derived from nine of eleven herbs of XFZY except Platycodon grandiflorum (Jacq.) A. DC. (Jiegeng) and Bupleurum chinense DC. (Chaihu). This UPLC method was validated in terms of linearity, LOD and LOQ, precision, repeatability, stability, and recovery tests. Quality control of XFZY products in total fourteen samples by four dosage forms was examined by this method, and results confirmed its feasibility and reliability in practice.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22264846</pmid><doi>10.1016/j.jpba.2011.12.026</doi><tpages>7</tpages></addata></record> |
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| subjects | amygdalin Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Bupleurum cardiovascular system Chromatography, Liquid - methods drug formulations Drugs, Chinese Herbal - chemistry ferulic acid Fundamental and applied biological sciences. Psychology General pharmacology glycyrrhizin herbs hesperidin hydroxymethylfurfural mass spectrometry Medical sciences naringin Oriental traditional medicine Pharmacology. Drug treatments Platycodon grandiflorum protective effect Qualitative analysis quality control Quantitative analysis Spectrometry, Mass, Electrospray Ionization - methods Tandem Mass Spectrometry - methods ultra-performance liquid chromatography UPLC-DAD UPLC–MS Xue Fu Zhu Yu |
| title | Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD–MS/MS |
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