Cartilage defect regeneration by ex vivo engineered autologous microtissue--preliminary results
We analysed the improvement of cartilage defect regeneration by the use of microspheres of autologous chondrocytes. Autologous chondrocytes from minipigs were cultured using the microsphere technology. Cartilage defects were surgically introduced to tibia-femoral joints. Tissue constructs were then...
Gespeichert in:
| Veröffentlicht in: | In vivo (Athens) 2012-03, Vol.26 (2), p.251-257 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 257 |
|---|---|
| container_issue | 2 |
| container_start_page | 251 |
| container_title | In vivo (Athens) |
| container_volume | 26 |
| creator | Meyer, Ulrich Wiesmann, Hans-Peter Libera, Jeanette Depprich, Rita Naujoks, Christian Handschel, Jörg |
| description | We analysed the improvement of cartilage defect regeneration by the use of microspheres of autologous chondrocytes. Autologous chondrocytes from minipigs were cultured using the microsphere technology. Cartilage defects were surgically introduced to tibia-femoral joints. Tissue constructs were then implanted into defect sites. Histological, immunohistological and transmission electron microscopic analyses were performed. Histological and ultrastructural investigations of chondrospheres revealed a cartilage-like tissue formation, indicated by phenotypically differentiated chondrocyte-like cells surrounded by de novo synthesised chondrogenic extracellular matrix. Clinical inspection of defects demonstrated nearly complete cartilage regeneration in the microtissue treated defect sites, whereas controls exhibited irregular fibrous tissue formation. In vitro-expanded articular chondrocytes are able to form a microtissue capable of repairing cartilage defects in vivo, improving regeneration of cartilage defects. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_922762804</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>922762804</sourcerecordid><originalsourceid>FETCH-LOGICAL-p210t-12d82ed77753ef15b8f93046079b9e9f67e7411ec71104686d9cc662d0641e93</originalsourceid><addsrcrecordid>eNo1kMtqwzAUREWhNGnaXyjadSWQZFuyliX0BYFssjeydG1UZMvVIzR_X0PT1cAwHA5zg7ZMKkZkU6sNuk_pi1IhKeV3aMN51TAhxBZ1ex2z83oEbGEAk3GEEWaIOrsw4_6C4Qef3TlgmEc3A0SwWJccfBhDSXhyJobsUipAyBLBu8nNOl5WTCo-pwd0O2if4PGaO3R6ez3tP8jh-P65fzmQhTOaCeO25WCllE0FA2v6dlAVrQWVqlegBiFB1oyBkYytdSusMkYIbqmoGahqh57_sEsM3wVS7iaXDHivZ1g1O8W5FLyl9bp8ui5LP4Htluim1bf7v6T6BbraXWM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>922762804</pqid></control><display><type>article</type><title>Cartilage defect regeneration by ex vivo engineered autologous microtissue--preliminary results</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Meyer, Ulrich ; Wiesmann, Hans-Peter ; Libera, Jeanette ; Depprich, Rita ; Naujoks, Christian ; Handschel, Jörg</creator><creatorcontrib>Meyer, Ulrich ; Wiesmann, Hans-Peter ; Libera, Jeanette ; Depprich, Rita ; Naujoks, Christian ; Handschel, Jörg</creatorcontrib><description>We analysed the improvement of cartilage defect regeneration by the use of microspheres of autologous chondrocytes. Autologous chondrocytes from minipigs were cultured using the microsphere technology. Cartilage defects were surgically introduced to tibia-femoral joints. Tissue constructs were then implanted into defect sites. Histological, immunohistological and transmission electron microscopic analyses were performed. Histological and ultrastructural investigations of chondrospheres revealed a cartilage-like tissue formation, indicated by phenotypically differentiated chondrocyte-like cells surrounded by de novo synthesised chondrogenic extracellular matrix. Clinical inspection of defects demonstrated nearly complete cartilage regeneration in the microtissue treated defect sites, whereas controls exhibited irregular fibrous tissue formation. In vitro-expanded articular chondrocytes are able to form a microtissue capable of repairing cartilage defects in vivo, improving regeneration of cartilage defects.</description><identifier>EISSN: 1791-7549</identifier><identifier>PMID: 22351666</identifier><language>eng</language><publisher>Greece</publisher><subject>Animals ; Cartilage, Articular - cytology ; Cells, Cultured - transplantation ; Chondrocytes - transplantation ; Chondrogenesis ; Extracellular Matrix - metabolism ; Hyaline Cartilage - injuries ; Hyaline Cartilage - physiology ; Male ; Microscopy, Electron, Transmission ; Microspheres ; Pilot Projects ; Recovery of Function ; Regeneration - physiology ; Spheroids, Cellular - transplantation ; Stifle - injuries ; Stifle - pathology ; Swine ; Swine, Miniature ; Tissue Engineering - methods ; Transplantation, Autologous ; Wounds and Injuries - surgery</subject><ispartof>In vivo (Athens), 2012-03, Vol.26 (2), p.251-257</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22351666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meyer, Ulrich</creatorcontrib><creatorcontrib>Wiesmann, Hans-Peter</creatorcontrib><creatorcontrib>Libera, Jeanette</creatorcontrib><creatorcontrib>Depprich, Rita</creatorcontrib><creatorcontrib>Naujoks, Christian</creatorcontrib><creatorcontrib>Handschel, Jörg</creatorcontrib><title>Cartilage defect regeneration by ex vivo engineered autologous microtissue--preliminary results</title><title>In vivo (Athens)</title><addtitle>In Vivo</addtitle><description>We analysed the improvement of cartilage defect regeneration by the use of microspheres of autologous chondrocytes. Autologous chondrocytes from minipigs were cultured using the microsphere technology. Cartilage defects were surgically introduced to tibia-femoral joints. Tissue constructs were then implanted into defect sites. Histological, immunohistological and transmission electron microscopic analyses were performed. Histological and ultrastructural investigations of chondrospheres revealed a cartilage-like tissue formation, indicated by phenotypically differentiated chondrocyte-like cells surrounded by de novo synthesised chondrogenic extracellular matrix. Clinical inspection of defects demonstrated nearly complete cartilage regeneration in the microtissue treated defect sites, whereas controls exhibited irregular fibrous tissue formation. In vitro-expanded articular chondrocytes are able to form a microtissue capable of repairing cartilage defects in vivo, improving regeneration of cartilage defects.</description><subject>Animals</subject><subject>Cartilage, Articular - cytology</subject><subject>Cells, Cultured - transplantation</subject><subject>Chondrocytes - transplantation</subject><subject>Chondrogenesis</subject><subject>Extracellular Matrix - metabolism</subject><subject>Hyaline Cartilage - injuries</subject><subject>Hyaline Cartilage - physiology</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microspheres</subject><subject>Pilot Projects</subject><subject>Recovery of Function</subject><subject>Regeneration - physiology</subject><subject>Spheroids, Cellular - transplantation</subject><subject>Stifle - injuries</subject><subject>Stifle - pathology</subject><subject>Swine</subject><subject>Swine, Miniature</subject><subject>Tissue Engineering - methods</subject><subject>Transplantation, Autologous</subject><subject>Wounds and Injuries - surgery</subject><issn>1791-7549</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtqwzAUREWhNGnaXyjadSWQZFuyliX0BYFssjeydG1UZMvVIzR_X0PT1cAwHA5zg7ZMKkZkU6sNuk_pi1IhKeV3aMN51TAhxBZ1ex2z83oEbGEAk3GEEWaIOrsw4_6C4Qef3TlgmEc3A0SwWJccfBhDSXhyJobsUipAyBLBu8nNOl5WTCo-pwd0O2if4PGaO3R6ez3tP8jh-P65fzmQhTOaCeO25WCllE0FA2v6dlAVrQWVqlegBiFB1oyBkYytdSusMkYIbqmoGahqh57_sEsM3wVS7iaXDHivZ1g1O8W5FLyl9bp8ui5LP4Htluim1bf7v6T6BbraXWM</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Meyer, Ulrich</creator><creator>Wiesmann, Hans-Peter</creator><creator>Libera, Jeanette</creator><creator>Depprich, Rita</creator><creator>Naujoks, Christian</creator><creator>Handschel, Jörg</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Cartilage defect regeneration by ex vivo engineered autologous microtissue--preliminary results</title><author>Meyer, Ulrich ; Wiesmann, Hans-Peter ; Libera, Jeanette ; Depprich, Rita ; Naujoks, Christian ; Handschel, Jörg</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p210t-12d82ed77753ef15b8f93046079b9e9f67e7411ec71104686d9cc662d0641e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Cartilage, Articular - cytology</topic><topic>Cells, Cultured - transplantation</topic><topic>Chondrocytes - transplantation</topic><topic>Chondrogenesis</topic><topic>Extracellular Matrix - metabolism</topic><topic>Hyaline Cartilage - injuries</topic><topic>Hyaline Cartilage - physiology</topic><topic>Male</topic><topic>Microscopy, Electron, Transmission</topic><topic>Microspheres</topic><topic>Pilot Projects</topic><topic>Recovery of Function</topic><topic>Regeneration - physiology</topic><topic>Spheroids, Cellular - transplantation</topic><topic>Stifle - injuries</topic><topic>Stifle - pathology</topic><topic>Swine</topic><topic>Swine, Miniature</topic><topic>Tissue Engineering - methods</topic><topic>Transplantation, Autologous</topic><topic>Wounds and Injuries - surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meyer, Ulrich</creatorcontrib><creatorcontrib>Wiesmann, Hans-Peter</creatorcontrib><creatorcontrib>Libera, Jeanette</creatorcontrib><creatorcontrib>Depprich, Rita</creatorcontrib><creatorcontrib>Naujoks, Christian</creatorcontrib><creatorcontrib>Handschel, Jörg</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>In vivo (Athens)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meyer, Ulrich</au><au>Wiesmann, Hans-Peter</au><au>Libera, Jeanette</au><au>Depprich, Rita</au><au>Naujoks, Christian</au><au>Handschel, Jörg</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cartilage defect regeneration by ex vivo engineered autologous microtissue--preliminary results</atitle><jtitle>In vivo (Athens)</jtitle><addtitle>In Vivo</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>26</volume><issue>2</issue><spage>251</spage><epage>257</epage><pages>251-257</pages><eissn>1791-7549</eissn><abstract>We analysed the improvement of cartilage defect regeneration by the use of microspheres of autologous chondrocytes. Autologous chondrocytes from minipigs were cultured using the microsphere technology. Cartilage defects were surgically introduced to tibia-femoral joints. Tissue constructs were then implanted into defect sites. Histological, immunohistological and transmission electron microscopic analyses were performed. Histological and ultrastructural investigations of chondrospheres revealed a cartilage-like tissue formation, indicated by phenotypically differentiated chondrocyte-like cells surrounded by de novo synthesised chondrogenic extracellular matrix. Clinical inspection of defects demonstrated nearly complete cartilage regeneration in the microtissue treated defect sites, whereas controls exhibited irregular fibrous tissue formation. In vitro-expanded articular chondrocytes are able to form a microtissue capable of repairing cartilage defects in vivo, improving regeneration of cartilage defects.</abstract><cop>Greece</cop><pmid>22351666</pmid><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1791-7549 |
| ispartof | In vivo (Athens), 2012-03, Vol.26 (2), p.251-257 |
| issn | 1791-7549 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_922762804 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Cartilage, Articular - cytology Cells, Cultured - transplantation Chondrocytes - transplantation Chondrogenesis Extracellular Matrix - metabolism Hyaline Cartilage - injuries Hyaline Cartilage - physiology Male Microscopy, Electron, Transmission Microspheres Pilot Projects Recovery of Function Regeneration - physiology Spheroids, Cellular - transplantation Stifle - injuries Stifle - pathology Swine Swine, Miniature Tissue Engineering - methods Transplantation, Autologous Wounds and Injuries - surgery |
| title | Cartilage defect regeneration by ex vivo engineered autologous microtissue--preliminary results |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T02%3A15%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cartilage%20defect%20regeneration%20by%20ex%20vivo%20engineered%20autologous%20microtissue--preliminary%20results&rft.jtitle=In%20vivo%20(Athens)&rft.au=Meyer,%20Ulrich&rft.date=2012-03-01&rft.volume=26&rft.issue=2&rft.spage=251&rft.epage=257&rft.pages=251-257&rft.eissn=1791-7549&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E922762804%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=922762804&rft_id=info:pmid/22351666&rfr_iscdi=true |