Neuroprotective Effect of Crocin on Acrylamide-induced Cytotoxicity in PC12 cells
Acrylamide (ACR) is a potent neurotoxic in human and animal models. In this study, the effect of crocin, main constituent of Crocus sativus L. (Saffron) on ACR-induced cytotoxicity was evaluated using PC12 cells as a suitable in vitro model. The exposure of PC12 cells to ACR reduced cell viability,...
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| Veröffentlicht in: | Cellular and molecular neurobiology 2012-03, Vol.32 (2), p.227-235 |
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| creator | Mehri, Soghra Abnous, Khalil Mousavi, Seyed Hadi Shariaty, Vahideh Motamed Hosseinzadeh, Hossein |
| description | Acrylamide (ACR) is a potent neurotoxic in human and animal models. In this study, the effect of crocin, main constituent of
Crocus sativus
L. (Saffron) on ACR-induced cytotoxicity was evaluated using PC12 cells as a suitable in vitro model. The exposure of PC12 cells to ACR reduced cell viability, increased DNA fragmented cells and phosphatidylserine exposure, and elevated Bax/Bcl-2 ratio. Results showed that ACR increased intracellular reactive oxygen species (ROS) in cells and ROS played an important role in ACR cytotoxicity. The pretreatment of cells with 10–50 μM crocin before ACR treatment significantly attenuated ACR cytotoxicity in a dose-dependent manner. Crocin inhibited the downregulation of Bcl-2 and the upregulation of Bax and decreased apoptosis in treated cells. Also, crocin inhibited ROS generation in cells exposed to ACR. In conclusion, our results indicated that pretreatment with crocin protected cells from ACR-induced apoptosis partly by inhibition of intracellular ROS production. |
| doi_str_mv | 10.1007/s10571-011-9752-8 |
| format | Article |
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Crocus sativus
L. (Saffron) on ACR-induced cytotoxicity was evaluated using PC12 cells as a suitable in vitro model. The exposure of PC12 cells to ACR reduced cell viability, increased DNA fragmented cells and phosphatidylserine exposure, and elevated Bax/Bcl-2 ratio. Results showed that ACR increased intracellular reactive oxygen species (ROS) in cells and ROS played an important role in ACR cytotoxicity. The pretreatment of cells with 10–50 μM crocin before ACR treatment significantly attenuated ACR cytotoxicity in a dose-dependent manner. Crocin inhibited the downregulation of Bcl-2 and the upregulation of Bax and decreased apoptosis in treated cells. Also, crocin inhibited ROS generation in cells exposed to ACR. In conclusion, our results indicated that pretreatment with crocin protected cells from ACR-induced apoptosis partly by inhibition of intracellular ROS production.</description><identifier>ISSN: 0272-4340</identifier><identifier>ISSN: 1573-6830</identifier><identifier>EISSN: 1573-6830</identifier><identifier>DOI: 10.1007/s10571-011-9752-8</identifier><identifier>PMID: 21901509</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Acrylamide - toxicity ; Animals ; bcl-2-Associated X Protein - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Carotenoids - pharmacology ; Cell Biology ; Cell Death - drug effects ; Cell Survival - drug effects ; DNA Fragmentation - drug effects ; Humans ; Neurobiology ; Neuroprotective Agents - pharmacology ; Neurosciences ; Original Research ; PC12 Cells ; Rats ; Reactive Oxygen Species - metabolism</subject><ispartof>Cellular and molecular neurobiology, 2012-03, Vol.32 (2), p.227-235</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-b6827ffcfd22b828496798786549a4d22acbfdb8d7168e222f339c82adcea5b93</citedby><cites>FETCH-LOGICAL-c409t-b6827ffcfd22b828496798786549a4d22acbfdb8d7168e222f339c82adcea5b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10571-011-9752-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10571-011-9752-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21901509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mehri, Soghra</creatorcontrib><creatorcontrib>Abnous, Khalil</creatorcontrib><creatorcontrib>Mousavi, Seyed Hadi</creatorcontrib><creatorcontrib>Shariaty, Vahideh Motamed</creatorcontrib><creatorcontrib>Hosseinzadeh, Hossein</creatorcontrib><title>Neuroprotective Effect of Crocin on Acrylamide-induced Cytotoxicity in PC12 cells</title><title>Cellular and molecular neurobiology</title><addtitle>Cell Mol Neurobiol</addtitle><addtitle>Cell Mol Neurobiol</addtitle><description>Acrylamide (ACR) is a potent neurotoxic in human and animal models. In this study, the effect of crocin, main constituent of
Crocus sativus
L. (Saffron) on ACR-induced cytotoxicity was evaluated using PC12 cells as a suitable in vitro model. The exposure of PC12 cells to ACR reduced cell viability, increased DNA fragmented cells and phosphatidylserine exposure, and elevated Bax/Bcl-2 ratio. Results showed that ACR increased intracellular reactive oxygen species (ROS) in cells and ROS played an important role in ACR cytotoxicity. The pretreatment of cells with 10–50 μM crocin before ACR treatment significantly attenuated ACR cytotoxicity in a dose-dependent manner. Crocin inhibited the downregulation of Bcl-2 and the upregulation of Bax and decreased apoptosis in treated cells. Also, crocin inhibited ROS generation in cells exposed to ACR. In conclusion, our results indicated that pretreatment with crocin protected cells from ACR-induced apoptosis partly by inhibition of intracellular ROS production.</description><subject>Acrylamide - toxicity</subject><subject>Animals</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carotenoids - pharmacology</subject><subject>Cell Biology</subject><subject>Cell Death - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>DNA Fragmentation - drug effects</subject><subject>Humans</subject><subject>Neurobiology</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Neurosciences</subject><subject>Original Research</subject><subject>PC12 Cells</subject><subject>Rats</subject><subject>Reactive Oxygen Species - metabolism</subject><issn>0272-4340</issn><issn>1573-6830</issn><issn>1573-6830</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EoqXwAWxQdqwM48nD9rKKykOqeEiwthLHRqnauNgJIn-PqxaWrDzynLmaOYRcMrhhAPw2MMg5o8AYlTxHKo7IlOU8pYVI4ZhMATnSLM1gQs5CWAGABMhPyQSZBJaDnJLXJzN4t_WuN7pvv0yysDZWibNJ6Z1uu8R1yVz7cV1t2sbQtmsGbZqkHHvXu-9Wt_2YROqlZJhos16Hc3Jiq3UwF4d3Rt7vFm_lA10-3z-W8yXVGcie1oVAbq22DWItUGSy4FJwUeSZrLL4WenaNrVoOCuEQUSbplILrBptqryW6Yxc73Pj8p-DCb3atGG3QdUZNwQlEXmBhYRIsj2pvQvBG6u2vt1UflQM1E6k2otUUaTaiVQizlwd0od6Y5q_iV9zEcA9EGKr-zBerdzgu3jxP6k_cA19xg</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Mehri, Soghra</creator><creator>Abnous, Khalil</creator><creator>Mousavi, Seyed Hadi</creator><creator>Shariaty, Vahideh Motamed</creator><creator>Hosseinzadeh, Hossein</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Neuroprotective Effect of Crocin on Acrylamide-induced Cytotoxicity in PC12 cells</title><author>Mehri, Soghra ; Abnous, Khalil ; Mousavi, Seyed Hadi ; Shariaty, Vahideh Motamed ; Hosseinzadeh, Hossein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-b6827ffcfd22b828496798786549a4d22acbfdb8d7168e222f339c82adcea5b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acrylamide - toxicity</topic><topic>Animals</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Carotenoids - pharmacology</topic><topic>Cell Biology</topic><topic>Cell Death - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>DNA Fragmentation - drug effects</topic><topic>Humans</topic><topic>Neurobiology</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Neurosciences</topic><topic>Original Research</topic><topic>PC12 Cells</topic><topic>Rats</topic><topic>Reactive Oxygen Species - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mehri, Soghra</creatorcontrib><creatorcontrib>Abnous, Khalil</creatorcontrib><creatorcontrib>Mousavi, Seyed Hadi</creatorcontrib><creatorcontrib>Shariaty, Vahideh Motamed</creatorcontrib><creatorcontrib>Hosseinzadeh, Hossein</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular and molecular neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mehri, Soghra</au><au>Abnous, Khalil</au><au>Mousavi, Seyed Hadi</au><au>Shariaty, Vahideh Motamed</au><au>Hosseinzadeh, Hossein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuroprotective Effect of Crocin on Acrylamide-induced Cytotoxicity in PC12 cells</atitle><jtitle>Cellular and molecular neurobiology</jtitle><stitle>Cell Mol Neurobiol</stitle><addtitle>Cell Mol Neurobiol</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>32</volume><issue>2</issue><spage>227</spage><epage>235</epage><pages>227-235</pages><issn>0272-4340</issn><issn>1573-6830</issn><eissn>1573-6830</eissn><abstract>Acrylamide (ACR) is a potent neurotoxic in human and animal models. In this study, the effect of crocin, main constituent of
Crocus sativus
L. (Saffron) on ACR-induced cytotoxicity was evaluated using PC12 cells as a suitable in vitro model. The exposure of PC12 cells to ACR reduced cell viability, increased DNA fragmented cells and phosphatidylserine exposure, and elevated Bax/Bcl-2 ratio. Results showed that ACR increased intracellular reactive oxygen species (ROS) in cells and ROS played an important role in ACR cytotoxicity. The pretreatment of cells with 10–50 μM crocin before ACR treatment significantly attenuated ACR cytotoxicity in a dose-dependent manner. Crocin inhibited the downregulation of Bcl-2 and the upregulation of Bax and decreased apoptosis in treated cells. Also, crocin inhibited ROS generation in cells exposed to ACR. In conclusion, our results indicated that pretreatment with crocin protected cells from ACR-induced apoptosis partly by inhibition of intracellular ROS production.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>21901509</pmid><doi>10.1007/s10571-011-9752-8</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Acrylamide - toxicity Animals bcl-2-Associated X Protein - metabolism Biomedical and Life Sciences Biomedicine Carotenoids - pharmacology Cell Biology Cell Death - drug effects Cell Survival - drug effects DNA Fragmentation - drug effects Humans Neurobiology Neuroprotective Agents - pharmacology Neurosciences Original Research PC12 Cells Rats Reactive Oxygen Species - metabolism |
| title | Neuroprotective Effect of Crocin on Acrylamide-induced Cytotoxicity in PC12 cells |
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