Capillary ion electrophoresis–capacitively coupled contactless conductivity detection of inorganic cations in human saliva on a polyvinyl alcohol-coated capillary
Capillary ion electrophoresis–capacitively coupled contactless conductivity detection (CIE-C4D) with a polyvinyl alcohol chemically coated capillary (PVA capillary) was used to analyze inorganic cations (Na + , K + , NH 4 + , Mg 2+ , and Ca 2+ ) commonly found in human saliva. The PVA capillary, whi...
Gespeichert in:
| Veröffentlicht in: | Analytical and bioanalytical chemistry 2012-03, Vol.402 (7), p.2425-2430 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2430 |
|---|---|
| container_issue | 7 |
| container_start_page | 2425 |
| container_title | Analytical and bioanalytical chemistry |
| container_volume | 402 |
| creator | Mori, Masanobu Kaseda, Maki Yamamoto, Tsukasa Yamada, Sachiko Itabashi, Hideyuki |
| description | Capillary ion electrophoresis–capacitively coupled contactless conductivity detection (CIE-C4D) with a polyvinyl alcohol chemically coated capillary (PVA capillary) was used to analyze inorganic cations (Na
+
, K
+
, NH
4
+
, Mg
2+
, and Ca
2+
) commonly found in human saliva. The PVA capillary, which was made by our laboratory, minimized electro-osmotic flow in the wide pH range of the background electrolyte (BGE), and the PVA layer adsorbed to capillary wall did not affect the conductimetric background level. In this study, we determined an optimized BGE of 30 mM lactic acid/histidine plus 3 mM 18-crown-6 for the CIE-C4D system using the PVA capillary, which could simultaneously improve the separation of Mg
2+
and Ca
2+
from Na
+
and that of K
+
from NH
4
+
. This system obtained highly reproducible separation of cations in human saliva samples within 8 min at 20 kV without deprotonation. The quantifiability of cations in human saliva samples on the CIE-C4D system was demonstrated through identification by ion chromatography with satisfactory results. |
| doi_str_mv | 10.1007/s00216-011-5688-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_922758493</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1221869380</sourcerecordid><originalsourceid>FETCH-LOGICAL-c541t-791dcee1742f2081ebf7cfe00c48b070c8128547556382e8909c7a7d6c62fd8d3</originalsourceid><addsrcrecordid>eNqNkj1u3DAQhYUgRuzYOUCagF3SKB5S4o_KYJE_wECauBa4o5GXBldUSGkBdb5DrpCT5SShsLZLx9UMht88PpCvKN5y-MgB9GUCEFyVwHkplTGlelGcccVNKZSEl499LU6L1yndAnBpuHpVnAohZGbUWfFnY0fnvY0Lc2Fg5AmnGMZdiJRc-nv3G-1o0U3uQH5hGObRU5frMFmcPKW09t2MGXDTwjqassCqFHrmhhBv7OCQoV1nKU_Ybt7bgSXr3cGyzFk2Br8c3LB4Zj2GXfAlBjuttzxYuyhOeusTvbmv58X1l88_N9_Kqx9fv28-XZUoaz6VuuEdEnFdi16A4bTtNfYEgLXZggY0XBhZaylVZQSZBhrUVncKleg701Xnxfuj7hjDr5nS1O5dQsoeBgpzahshtDR1U2Xyw5Nk9lBrwaFqnoeC1gr-jwrBjWoq8wwUTIaVbExG-RHFGFKK1LdjdPv8rBlq1xy1xxy1OUftmqNW5Z139_Lzdk_d48ZDcDIgjkDKR8MNxfY2zHHIv_OE6j-mOtbS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1082216598</pqid></control><display><type>article</type><title>Capillary ion electrophoresis–capacitively coupled contactless conductivity detection of inorganic cations in human saliva on a polyvinyl alcohol-coated capillary</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Mori, Masanobu ; Kaseda, Maki ; Yamamoto, Tsukasa ; Yamada, Sachiko ; Itabashi, Hideyuki</creator><creatorcontrib>Mori, Masanobu ; Kaseda, Maki ; Yamamoto, Tsukasa ; Yamada, Sachiko ; Itabashi, Hideyuki</creatorcontrib><description>Capillary ion electrophoresis–capacitively coupled contactless conductivity detection (CIE-C4D) with a polyvinyl alcohol chemically coated capillary (PVA capillary) was used to analyze inorganic cations (Na
+
, K
+
, NH
4
+
, Mg
2+
, and Ca
2+
) commonly found in human saliva. The PVA capillary, which was made by our laboratory, minimized electro-osmotic flow in the wide pH range of the background electrolyte (BGE), and the PVA layer adsorbed to capillary wall did not affect the conductimetric background level. In this study, we determined an optimized BGE of 30 mM lactic acid/histidine plus 3 mM 18-crown-6 for the CIE-C4D system using the PVA capillary, which could simultaneously improve the separation of Mg
2+
and Ca
2+
from Na
+
and that of K
+
from NH
4
+
. This system obtained highly reproducible separation of cations in human saliva samples within 8 min at 20 kV without deprotonation. The quantifiability of cations in human saliva samples on the CIE-C4D system was demonstrated through identification by ion chromatography with satisfactory results.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-011-5688-6</identifier><identifier>PMID: 22252656</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Biochemistry ; Capillarity ; Cations ; Cations - analysis ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Electrophoresis, Capillary - methods ; Food Science ; Histidine ; Human ; Humans ; Inorganic Chemicals - analysis ; Laboratory Medicine ; Monitoring/Environmental Analysis ; Original Paper ; Polyvinyl Alcohol - chemistry ; Polyvinyl alcohols ; Saliva ; Separation ; Walls</subject><ispartof>Analytical and bioanalytical chemistry, 2012-03, Vol.402 (7), p.2425-2430</ispartof><rights>Springer-Verlag 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-791dcee1742f2081ebf7cfe00c48b070c8128547556382e8909c7a7d6c62fd8d3</citedby><cites>FETCH-LOGICAL-c541t-791dcee1742f2081ebf7cfe00c48b070c8128547556382e8909c7a7d6c62fd8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-011-5688-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-011-5688-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22252656$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mori, Masanobu</creatorcontrib><creatorcontrib>Kaseda, Maki</creatorcontrib><creatorcontrib>Yamamoto, Tsukasa</creatorcontrib><creatorcontrib>Yamada, Sachiko</creatorcontrib><creatorcontrib>Itabashi, Hideyuki</creatorcontrib><title>Capillary ion electrophoresis–capacitively coupled contactless conductivity detection of inorganic cations in human saliva on a polyvinyl alcohol-coated capillary</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Capillary ion electrophoresis–capacitively coupled contactless conductivity detection (CIE-C4D) with a polyvinyl alcohol chemically coated capillary (PVA capillary) was used to analyze inorganic cations (Na
+
, K
+
, NH
4
+
, Mg
2+
, and Ca
2+
) commonly found in human saliva. The PVA capillary, which was made by our laboratory, minimized electro-osmotic flow in the wide pH range of the background electrolyte (BGE), and the PVA layer adsorbed to capillary wall did not affect the conductimetric background level. In this study, we determined an optimized BGE of 30 mM lactic acid/histidine plus 3 mM 18-crown-6 for the CIE-C4D system using the PVA capillary, which could simultaneously improve the separation of Mg
2+
and Ca
2+
from Na
+
and that of K
+
from NH
4
+
. This system obtained highly reproducible separation of cations in human saliva samples within 8 min at 20 kV without deprotonation. The quantifiability of cations in human saliva samples on the CIE-C4D system was demonstrated through identification by ion chromatography with satisfactory results.</description><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Capillarity</subject><subject>Cations</subject><subject>Cations - analysis</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Food Science</subject><subject>Histidine</subject><subject>Human</subject><subject>Humans</subject><subject>Inorganic Chemicals - analysis</subject><subject>Laboratory Medicine</subject><subject>Monitoring/Environmental Analysis</subject><subject>Original Paper</subject><subject>Polyvinyl Alcohol - chemistry</subject><subject>Polyvinyl alcohols</subject><subject>Saliva</subject><subject>Separation</subject><subject>Walls</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkj1u3DAQhYUgRuzYOUCagF3SKB5S4o_KYJE_wECauBa4o5GXBldUSGkBdb5DrpCT5SShsLZLx9UMht88PpCvKN5y-MgB9GUCEFyVwHkplTGlelGcccVNKZSEl499LU6L1yndAnBpuHpVnAohZGbUWfFnY0fnvY0Lc2Fg5AmnGMZdiJRc-nv3G-1o0U3uQH5hGObRU5frMFmcPKW09t2MGXDTwjqassCqFHrmhhBv7OCQoV1nKU_Ybt7bgSXr3cGyzFk2Br8c3LB4Zj2GXfAlBjuttzxYuyhOeusTvbmv58X1l88_N9_Kqx9fv28-XZUoaz6VuuEdEnFdi16A4bTtNfYEgLXZggY0XBhZaylVZQSZBhrUVncKleg701Xnxfuj7hjDr5nS1O5dQsoeBgpzahshtDR1U2Xyw5Nk9lBrwaFqnoeC1gr-jwrBjWoq8wwUTIaVbExG-RHFGFKK1LdjdPv8rBlq1xy1xxy1OUftmqNW5Z139_Lzdk_d48ZDcDIgjkDKR8MNxfY2zHHIv_OE6j-mOtbS</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Mori, Masanobu</creator><creator>Kaseda, Maki</creator><creator>Yamamoto, Tsukasa</creator><creator>Yamada, Sachiko</creator><creator>Itabashi, Hideyuki</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7UA</scope><scope>C1K</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Capillary ion electrophoresis–capacitively coupled contactless conductivity detection of inorganic cations in human saliva on a polyvinyl alcohol-coated capillary</title><author>Mori, Masanobu ; Kaseda, Maki ; Yamamoto, Tsukasa ; Yamada, Sachiko ; Itabashi, Hideyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-791dcee1742f2081ebf7cfe00c48b070c8128547556382e8909c7a7d6c62fd8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical Chemistry</topic><topic>Biochemistry</topic><topic>Capillarity</topic><topic>Cations</topic><topic>Cations - analysis</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Food Science</topic><topic>Histidine</topic><topic>Human</topic><topic>Humans</topic><topic>Inorganic Chemicals - analysis</topic><topic>Laboratory Medicine</topic><topic>Monitoring/Environmental Analysis</topic><topic>Original Paper</topic><topic>Polyvinyl Alcohol - chemistry</topic><topic>Polyvinyl alcohols</topic><topic>Saliva</topic><topic>Separation</topic><topic>Walls</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mori, Masanobu</creatorcontrib><creatorcontrib>Kaseda, Maki</creatorcontrib><creatorcontrib>Yamamoto, Tsukasa</creatorcontrib><creatorcontrib>Yamada, Sachiko</creatorcontrib><creatorcontrib>Itabashi, Hideyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mori, Masanobu</au><au>Kaseda, Maki</au><au>Yamamoto, Tsukasa</au><au>Yamada, Sachiko</au><au>Itabashi, Hideyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary ion electrophoresis–capacitively coupled contactless conductivity detection of inorganic cations in human saliva on a polyvinyl alcohol-coated capillary</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>402</volume><issue>7</issue><spage>2425</spage><epage>2430</epage><pages>2425-2430</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Capillary ion electrophoresis–capacitively coupled contactless conductivity detection (CIE-C4D) with a polyvinyl alcohol chemically coated capillary (PVA capillary) was used to analyze inorganic cations (Na
+
, K
+
, NH
4
+
, Mg
2+
, and Ca
2+
) commonly found in human saliva. The PVA capillary, which was made by our laboratory, minimized electro-osmotic flow in the wide pH range of the background electrolyte (BGE), and the PVA layer adsorbed to capillary wall did not affect the conductimetric background level. In this study, we determined an optimized BGE of 30 mM lactic acid/histidine plus 3 mM 18-crown-6 for the CIE-C4D system using the PVA capillary, which could simultaneously improve the separation of Mg
2+
and Ca
2+
from Na
+
and that of K
+
from NH
4
+
. This system obtained highly reproducible separation of cations in human saliva samples within 8 min at 20 kV without deprotonation. The quantifiability of cations in human saliva samples on the CIE-C4D system was demonstrated through identification by ion chromatography with satisfactory results.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22252656</pmid><doi>10.1007/s00216-011-5688-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2012-03, Vol.402 (7), p.2425-2430 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_922758493 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Analytical Chemistry Biochemistry Capillarity Cations Cations - analysis Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Electrophoresis, Capillary - methods Food Science Histidine Human Humans Inorganic Chemicals - analysis Laboratory Medicine Monitoring/Environmental Analysis Original Paper Polyvinyl Alcohol - chemistry Polyvinyl alcohols Saliva Separation Walls |
| title | Capillary ion electrophoresis–capacitively coupled contactless conductivity detection of inorganic cations in human saliva on a polyvinyl alcohol-coated capillary |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T10%3A58%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Capillary%20ion%20electrophoresis%E2%80%93capacitively%20coupled%20contactless%20conductivity%20detection%20of%20inorganic%20cations%20in%20human%20saliva%20on%20a%20polyvinyl%20alcohol-coated%20capillary&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Mori,%20Masanobu&rft.date=2012-03-01&rft.volume=402&rft.issue=7&rft.spage=2425&rft.epage=2430&rft.pages=2425-2430&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-011-5688-6&rft_dat=%3Cproquest_cross%3E1221869380%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1082216598&rft_id=info:pmid/22252656&rfr_iscdi=true |