Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells

► We reconstituted chaperonin CCT, a 16-mer complex of 8 distinct subunits. ► The recombinant CCT was morphologically indistinguishable from the native CCT. ► The recombinant CCT was functionally indistinguishable from the native CCT. The eukaryotic cytosolic chaperonin CCT (chaperonin-containing TC...

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Veröffentlicht in:	Protein expression and purification 2012-03, Vol.82 (1), p.61-69
Hauptverfasser:	Machida, Kodai, Masutani, Mamiko, Kobayashi, Tominari, Mikami, Satoshi, Nishino, Yuri, Miyazawa, Atsuo, Imataka, Hiroaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals CCT Cell Line Chaperonin Chaperonin Containing TCP-1 - chemistry Chaperonin Containing TCP-1 - genetics Chaperonin Containing TCP-1 - isolation & purification Gene Expression Humans Molecular Sequence Data Plasmids - genetics Protein complex Protein Folding Protein Subunits - chemistry Protein Subunits - genetics Protein Subunits - isolation & purification Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Reconstitution Vaccinia virus Vaccinia virus - genetics
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