Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research
Large-insert BAC (bacterial artificial chromosome) and BIBAC (binary BAC) libraries are essential for modern genomics research for all organisms. We helped pioneer the BAC and BIBAC technologies, and by using them we have constructed hundreds of BAC and BIBAC libraries for different species of plant...
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| Veröffentlicht in: | Nature protocols 2012-03, Vol.7 (3), p.479-499 |
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| creator | Zhang, Hong-Bin Scheuring, Chantel F Zhang, Meiping Zhang, Yang Wu, Cheng-Cang Dong, Jennifer J Li, Yaning |
| description | Large-insert BAC (bacterial artificial chromosome) and BIBAC (binary BAC) libraries are essential for modern genomics research for all organisms. We helped pioneer the BAC and BIBAC technologies, and by using them we have constructed hundreds of BAC and BIBAC libraries for different species of plants, animals, marine animals, insects, algae and microbes. These libraries have been used globally for different aspects of genomics research. Here we describe the procedure with the latest improvements that we have made and used for construction of BIBAC libraries. The procedure includes the preparation of BIBAC vectors, the preparation of clonable fragments of the desired size from the source DNA, the construction and transformation of BIBACs and, finally, the characterization and assembly of BIBAC libraries. We also specify the modifications necessary for construction of BAC libraries using the protocol. The entire protocol takes ∼7 d. |
| doi_str_mv | 10.1038/nprot.2011.456 |
| format | Article |
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We helped pioneer the BAC and BIBAC technologies, and by using them we have constructed hundreds of BAC and BIBAC libraries for different species of plants, animals, marine animals, insects, algae and microbes. These libraries have been used globally for different aspects of genomics research. Here we describe the procedure with the latest improvements that we have made and used for construction of BIBAC libraries. The procedure includes the preparation of BIBAC vectors, the preparation of clonable fragments of the desired size from the source DNA, the construction and transformation of BIBACs and, finally, the characterization and assembly of BIBAC libraries. We also specify the modifications necessary for construction of BAC libraries using the protocol. The entire protocol takes ∼7 d.</description><identifier>ISSN: 1754-2189</identifier><identifier>EISSN: 1750-2799</identifier><identifier>DOI: 10.1038/nprot.2011.456</identifier><identifier>PMID: 22343430</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/2300/1514 ; 631/1647/666/2261 ; Algae ; Analysis ; Analytical Chemistry ; Animals ; Artificial chromosomes ; Bacterial artificial chromosomes ; Bioengineering ; Biological Techniques ; Biomedical and Life Sciences ; Chromosomes, Artificial, Bacterial - genetics ; Cloning ; Computational Biology/Bioinformatics ; Construction ; Deoxyribonucleic acid ; DNA ; DNA - isolation & purification ; Gene Library ; Genetic aspects ; Genetic Vectors - genetics ; Genomes ; Genomics ; Genomics - methods ; Insects ; Life Sciences ; Marine organisms ; Microarrays ; Organic Chemistry ; Organisms ; Physiological aspects ; Plant species ; Plasmids ; Protocol ; Recombinant DNA ; Species Specificity ; Transformation ; Transgenic plants ; Yeast fungi</subject><ispartof>Nature protocols, 2012-03, Vol.7 (3), p.479-499</ispartof><rights>Springer Nature Limited 2012</rights><rights>COPYRIGHT 2012 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-3b3b5b2b99d5783c658023f3238532d0daec0978584de7cf1c03df277ba24ab13</citedby><cites>FETCH-LOGICAL-c457t-3b3b5b2b99d5783c658023f3238532d0daec0978584de7cf1c03df277ba24ab13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nprot.2011.456$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nprot.2011.456$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22343430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hong-Bin</creatorcontrib><creatorcontrib>Scheuring, Chantel F</creatorcontrib><creatorcontrib>Zhang, Meiping</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Wu, Cheng-Cang</creatorcontrib><creatorcontrib>Dong, Jennifer J</creatorcontrib><creatorcontrib>Li, Yaning</creatorcontrib><title>Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research</title><title>Nature protocols</title><addtitle>Nat Protoc</addtitle><addtitle>Nat Protoc</addtitle><description>Large-insert BAC (bacterial artificial chromosome) and BIBAC (binary BAC) libraries are essential for modern genomics research for all organisms. 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The entire protocol takes ∼7 d.</description><subject>631/1647/2300/1514</subject><subject>631/1647/666/2261</subject><subject>Algae</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Artificial chromosomes</subject><subject>Bacterial artificial chromosomes</subject><subject>Bioengineering</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Chromosomes, Artificial, Bacterial - genetics</subject><subject>Cloning</subject><subject>Computational Biology/Bioinformatics</subject><subject>Construction</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - isolation & purification</subject><subject>Gene Library</subject><subject>Genetic aspects</subject><subject>Genetic Vectors - genetics</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Genomics - methods</subject><subject>Insects</subject><subject>Life Sciences</subject><subject>Marine organisms</subject><subject>Microarrays</subject><subject>Organic Chemistry</subject><subject>Organisms</subject><subject>Physiological aspects</subject><subject>Plant species</subject><subject>Plasmids</subject><subject>Protocol</subject><subject>Recombinant DNA</subject><subject>Species Specificity</subject><subject>Transformation</subject><subject>Transgenic plants</subject><subject>Yeast fungi</subject><issn>1754-2189</issn><issn>1750-2799</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUtv1DAUhS0EomVgyxJZYlFYZOpnbC-nI6AjVULisY4c2wmpErvYTkX_Pc5MobRClRfX1v10j889ALzGaI0Rlaf-Koa8JgjjNeP1E3CMBUcVEUo93d9ZRbBUR-BFSpcIMUFr8RwcEUJZOegYtNvgU46zyUPwMHTwbHe22ULtLVzqOLRRx8El2MUwQQ2vl1e-WcgQe-2HNJVeiFDba-2Ns7B3PkyDSTC65HQ0P16CZ50ek3t1W1fg-8cP37bn1cXnT7vt5qIyjItc0Za2vCWtUpYLSU3NJSK0o4RKTolFVjuDlJBcMuuE6bBB1HZEiFYTpltMV-DkMLes5OfsUm6mIRk3jtq7MKdGEcKUkrwu5LtHSYxI0ZbLilbg7QP0MszRFx-FoooqRLG8o3o9umbwXchRm2Vos6FKkLqWnBfq_T3KBJ_dr9zrOaVm9_XLfXZ9YE0MKUXXNVdxmHS8KbKLsmz2yTdL8g3be3pz-9G5nZz9i_-JugCnByCVlu9d_NfJf0f-BpH_tmw</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Zhang, Hong-Bin</creator><creator>Scheuring, Chantel F</creator><creator>Zhang, Meiping</creator><creator>Zhang, Yang</creator><creator>Wu, Cheng-Cang</creator><creator>Dong, Jennifer J</creator><creator>Li, Yaning</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research</title><author>Zhang, Hong-Bin ; Scheuring, Chantel F ; Zhang, Meiping ; Zhang, Yang ; Wu, Cheng-Cang ; Dong, Jennifer J ; Li, Yaning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-3b3b5b2b99d5783c658023f3238532d0daec0978584de7cf1c03df277ba24ab13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>631/1647/2300/1514</topic><topic>631/1647/666/2261</topic><topic>Algae</topic><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Artificial chromosomes</topic><topic>Bacterial artificial chromosomes</topic><topic>Bioengineering</topic><topic>Biological Techniques</topic><topic>Biomedical and Life Sciences</topic><topic>Chromosomes, Artificial, Bacterial - genetics</topic><topic>Cloning</topic><topic>Computational Biology/Bioinformatics</topic><topic>Construction</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - isolation & purification</topic><topic>Gene Library</topic><topic>Genetic aspects</topic><topic>Genetic Vectors - genetics</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Genomics - methods</topic><topic>Insects</topic><topic>Life Sciences</topic><topic>Marine organisms</topic><topic>Microarrays</topic><topic>Organic Chemistry</topic><topic>Organisms</topic><topic>Physiological aspects</topic><topic>Plant species</topic><topic>Plasmids</topic><topic>Protocol</topic><topic>Recombinant DNA</topic><topic>Species Specificity</topic><topic>Transformation</topic><topic>Transgenic plants</topic><topic>Yeast fungi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hong-Bin</creatorcontrib><creatorcontrib>Scheuring, Chantel F</creatorcontrib><creatorcontrib>Zhang, Meiping</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Wu, Cheng-Cang</creatorcontrib><creatorcontrib>Dong, Jennifer J</creatorcontrib><creatorcontrib>Li, Yaning</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature protocols</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hong-Bin</au><au>Scheuring, Chantel F</au><au>Zhang, Meiping</au><au>Zhang, Yang</au><au>Wu, Cheng-Cang</au><au>Dong, Jennifer J</au><au>Li, Yaning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research</atitle><jtitle>Nature protocols</jtitle><stitle>Nat Protoc</stitle><addtitle>Nat Protoc</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>7</volume><issue>3</issue><spage>479</spage><epage>499</epage><pages>479-499</pages><issn>1754-2189</issn><eissn>1750-2799</eissn><abstract>Large-insert BAC (bacterial artificial chromosome) and BIBAC (binary BAC) libraries are essential for modern genomics research for all organisms. We helped pioneer the BAC and BIBAC technologies, and by using them we have constructed hundreds of BAC and BIBAC libraries for different species of plants, animals, marine animals, insects, algae and microbes. These libraries have been used globally for different aspects of genomics research. Here we describe the procedure with the latest improvements that we have made and used for construction of BIBAC libraries. The procedure includes the preparation of BIBAC vectors, the preparation of clonable fragments of the desired size from the source DNA, the construction and transformation of BIBACs and, finally, the characterization and assembly of BIBAC libraries. We also specify the modifications necessary for construction of BAC libraries using the protocol. The entire protocol takes ∼7 d.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>22343430</pmid><doi>10.1038/nprot.2011.456</doi><tpages>21</tpages></addata></record> |
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| source | MEDLINE; SpringerLink Journals; Nature Journals Online |
| subjects | 631/1647/2300/1514 631/1647/666/2261 Algae Analysis Analytical Chemistry Animals Artificial chromosomes Bacterial artificial chromosomes Bioengineering Biological Techniques Biomedical and Life Sciences Chromosomes, Artificial, Bacterial - genetics Cloning Computational Biology/Bioinformatics Construction Deoxyribonucleic acid DNA DNA - isolation & purification Gene Library Genetic aspects Genetic Vectors - genetics Genomes Genomics Genomics - methods Insects Life Sciences Marine organisms Microarrays Organic Chemistry Organisms Physiological aspects Plant species Plasmids Protocol Recombinant DNA Species Specificity Transformation Transgenic plants Yeast fungi |
| title | Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research |
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