Prevalence of enteropathogens in normal feces from healthy children at an infant day care in Brazil

The diarrhea associated with gastroenteritis is a major cause of morbidity and mortality worldwide, affecting mainly infants. The characterization of both viral and bacterial agents associated with gastroenteritis can establish policies for surveillance, prevention and treatment of infections. Group...

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Veröffentlicht in:Journal of infection in developing countries 2012-02, Vol.6 (2), p.176-180
Hauptverfasser: de Moura, Cláudia, Fregolente, Maria Clara Duarte, Martini, Isabel Julien, Domingos, Daniela Ferreira, da Silva, Erivaldo José, Ferraz, Mirtis Maria Giaciani, Gatti, Maria Silvia Viccari, da Silva Leite, Domingos
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container_end_page 180
container_issue 2
container_start_page 176
container_title Journal of infection in developing countries
container_volume 6
creator de Moura, Cláudia
Fregolente, Maria Clara Duarte
Martini, Isabel Julien
Domingos, Daniela Ferreira
da Silva, Erivaldo José
Ferraz, Mirtis Maria Giaciani
Gatti, Maria Silvia Viccari
da Silva Leite, Domingos
description The diarrhea associated with gastroenteritis is a major cause of morbidity and mortality worldwide, affecting mainly infants. The characterization of both viral and bacterial agents associated with gastroenteritis can establish policies for surveillance, prevention and treatment of infections. Group A rotaviruses are the major infectious agent associated with dehydration in children, followed by pathotypes of Escherichia coli. There are three main types of clinical infections caused by E. coli strains that have acquired virulence genes: (i) enteric and diarrheal diseases, (ii) urinary tract infections, and (iii) sepsis and meningitis. In this study, the objective was to identify the presence of rotavirus and diarrhogenic E. coli in the feces of children 4 to 14 months of age who displayed no gastroenteritis symptoms and stayed all day in a day-care center. We analyzed 188 samples using PAGE and PCR to identify rotaviruses and E. coli virulence genes, respectively. Thirty-six samples (19.1%) were positive for at least one pathotype of E. coli. Nineteen were identified to be of the EPEC group and fifteen of the EAEC group. Rotaviruses were not identified. As EPEC and EAEC are potential pathogens for children less than one year of age or immunocompromised individuals, our results show the importance of appropriate monitoring by public health agencies. In the situation that we have studied, children can be considered asymptomatic carriers of these pathogens and can transmit them to other susceptible children.
doi_str_mv 10.3855/jidc.1982
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The characterization of both viral and bacterial agents associated with gastroenteritis can establish policies for surveillance, prevention and treatment of infections. Group A rotaviruses are the major infectious agent associated with dehydration in children, followed by pathotypes of Escherichia coli. There are three main types of clinical infections caused by E. coli strains that have acquired virulence genes: (i) enteric and diarrheal diseases, (ii) urinary tract infections, and (iii) sepsis and meningitis. In this study, the objective was to identify the presence of rotavirus and diarrhogenic E. coli in the feces of children 4 to 14 months of age who displayed no gastroenteritis symptoms and stayed all day in a day-care center. We analyzed 188 samples using PAGE and PCR to identify rotaviruses and E. coli virulence genes, respectively. Thirty-six samples (19.1%) were positive for at least one pathotype of E. coli. Nineteen were identified to be of the EPEC group and fifteen of the EAEC group. Rotaviruses were not identified. As EPEC and EAEC are potential pathogens for children less than one year of age or immunocompromised individuals, our results show the importance of appropriate monitoring by public health agencies. In the situation that we have studied, children can be considered asymptomatic carriers of these pathogens and can transmit them to other susceptible children.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.1982</identifier><identifier>PMID: 22337847</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Brazil ; Carrier State - epidemiology ; Carrier State - microbiology ; Child Day Care Centers ; E coli ; Escherichia coli - isolation &amp; purification ; Feces - microbiology ; Female ; Gastroenteritis ; Humans ; Infant ; Infections ; Male ; Pathogens ; Prevalence ; Rotavirus - isolation &amp; purification ; Virulence</subject><ispartof>Journal of infection in developing countries, 2012-02, Vol.6 (2), p.176-180</ispartof><rights>2012. 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Nineteen were identified to be of the EPEC group and fifteen of the EAEC group. Rotaviruses were not identified. 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identifier ISSN: 1972-2680
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subjects Brazil
Carrier State - epidemiology
Carrier State - microbiology
Child Day Care Centers
E coli
Escherichia coli - isolation & purification
Feces - microbiology
Female
Gastroenteritis
Humans
Infant
Infections
Male
Pathogens
Prevalence
Rotavirus - isolation & purification
Virulence
title Prevalence of enteropathogens in normal feces from healthy children at an infant day care in Brazil
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